HPRT Antibody

Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Rat colon. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: HPRT Antibody [NBP1-33527] - A. 50 ug mouse liver lysate/extract 12 % SDS-PAGEHPRT antibody dilution: 1:500

Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: HPRT Antibody [NBP1-33527] - Wild-type (WT) and HPRT knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Human endometrial cancer. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Colon cancer. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Rat brain. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Mouse colon. HPRT antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca, Ch, Pm, Rb, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

HPRT Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human HPRT. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Porcine (97%), Chimpanzee (99%), Bovine (94%), Rabbit (98%), Zebrafish (93%), Chicken (94%), Canine (96%), Xenopus (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HPRT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for HPRT Antibody

• EC 2.4.2.8
• HGPRT
• HGPRT1
• HGPRTHPRTHGPRTase
• HPRT
• HPRT1
• hypoxanthine phosphoribosyltransferase 1
• hypoxanthine-guanine phosphoribosyltransferase

Background

The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPRT Antibody (NBP1-33527)(3)

Publications using NBP1-33527

• Liu Y, Andreucci A, Iwamoto N et al. Preclinical evaluation of WVE-004, aninvestigational stereopure oligonucleotide forthe treatment of C9orf72-associated ALS or FTD Molecular Therapy - Nucleic Acids [PMID: 35592494]
• Hagiwara K, Honma M, Harumoto T et al. Development of Prodrug Type Circular siRNA for In Vivo Knockdown by Systemic Administration Nucleic Acid Ther 2020-10-05 [PMID: 33016851]
• Doigneaux C, Pedley AM, Mistry IN et al. Hypoxia Drives the Assembly of the Multi-Enzyme Purinosome Complex J. Biol. Chem. 2020-05-21 [PMID: 32439803] (WB, Human)

Bioinformatics

• Gene Symbol: HPRT1