Beta-Actin's Role in Neuronal Plasticity

Thu, 02/04/2016 - 14:29

Beta-Actin is a highly conserved protein involved in cell growth, cytoskeletal and extracellular support structures and cell migration. Because beta-Actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blots.

Interestingly, Beta-Actin has been shown to be associated with growth cones in developing neurite cells. While beta-Actin is one of several actins involved in the guidance of growth cones towards synaptic targets, beta-Actin mRNA has also been shown to regulate protein synthesis by binding to Vg1RBP, ultimately resulting in asymmetrical translation of beta-Actin near the site of growth cone turning.1 Beta-actin has also been shown to localize in areas associated with neuronal growth and remodeling in both developing and mature neuronal cell types. Beta-actin is primarily found in the growth cones, filopodia, cell bodies and axonal tracks of developing cerebellar cultures with the cytoplasmic concentration rapidly decreasing with age. In mature cultures, beta-actin is found to be primarily localized in dendritic spines, structures which are involved in plasticity in mature cells. 2

Novus Biologicals offers beta-Actin antibodies for most applications and in multiple pre-conjugated formats. Our beta-Actin antibodies have been cited hundreds of times and have received multiple 5 star customer reviews.

We offer the Novus 100% Guarantee that each antibody will work in the species and applications given on the product datasheet. We also offer beta-Actin over expression lysates, recombinant proteins and RNAis.

beta-Actin Antibody (AC-15) [NB600-501]

beta-Actin Antibody[NB600-503]

beta-Actin Antibody

IHC image of beta-Actin

Immunofluorescence analysis of 
beta-Actin in HS-68 cells.

Simple Western lane view 
shows a specific band for 
beta-Actin in 0.1 mg/ml of 
HeLa lysate.

Western blot analysis using lysates from A: human brain, B: mouse brain, C: rat brain, D: human lung and E: human spleen tissue probed using beta-actin antibody.

  1. [PMID: 16980963]
  2. [PMID: 9875357]

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