Sordaria macrospora is a filamentous fungus that serves as very useful system for scientific research due to a short life cycle and easy manipulation. Just like any other model organism, it is important to have an effective loading control to validate experiments in the Sordaria macrospora. In addition, the growth and morphogenesis of filamentous fungi is dependent on actin organization. Actin is a very abundant protein across biological species and can transition between monomeric and filamentous states. Actin has an alpha and beta isoform, both of which sha
Beta-actin belongs to a large family of highly conserved structural cell proteins that regulate cell motility, structure, and integrity. Beta-actin is expressed in all eukaryotic cells making it the ideal internal quantitative control for protein comparative assays. This feature has made it uniquely a historical and heavily-utilized standard, as the public record of scientific publication literature can attest to.
Beta Actin is one isoform of a multifamily of highly conserved proteins that regulate cell motility, structure, and integrity. The ubiquitous expression of beta-actin in all eukaryotic cells makes it both a historical and heavily-used internal quantitative control for protein comparative assays, as can be seen in the scientific literature and publication records.
Actins are an essential component of the cytoskeleton, with critical roles in a wide range of cellular processes, including cell migration, division, and regulation of gene expression. These functions are attributed to the ability of actin to form filaments that can rapidly assemble and disassemble according to the needs of the cell. There exist six different but highly conserved actin isoforms in vertebrates (1).
Actins are highly conserved, commonly found and abundant proteins involved in several types of cell motility as well as cytoskeleton maintenance. In vertebrate species, three main groups of actin isoforms, the alpha, beta and gamma, have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus.
Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. A loading control antibody helps determine if samples have been loaded equally across all wells and confirms effective protein transfer during the western blot protocol.
One of six actin isoforms so far identified, beta actin is a highly conserved cytoskeletal protein involved in cell structure, motility, and cohesion. We at Novus Biologicals have many different beta-actin products in our catalog, used in areas such as cytoskeleton and signal transduction research. They are also used as loading controls in protein assays. Beta-actin is known as a “housekeeping” protein, i.e.
Antibody conjugation involves tagging on a protein, compound or dye, in order to track its interaction with specific antigens. It is a routine procedure in immunoassays. We at Novus Biologicals have a vast number of products in our conjugated antibody catalog, to which we are constantly adding.