beta 2-Microglobulin Antibody (B2M-01) - BSA Free

Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.

Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Confocal microscopy of human beta2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.

Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Staining of beta-2-microglobulin in human peripheral blood with anti-beta-2-microglobulin (B2M-01) biotin.

Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) FITC.

Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PerCP-CyTM5.5.

Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PE.

ELISA: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis of HLA-G and beta2-microglobulin in supernatant of HLA-G transfectants with anti-beta2-microglobulin (B2M-01) HRP as the detection antibody. Image using the HRP format of this antibody.

• Reactivity: Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA
• Clone: B2M-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

beta 2-Microglobulin Antibody (B2M-01) - BSA Free Summary

• Immunogen: Purified human beta 2 Microglobulin (NM_004048.2).
• Specificity: The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: B2M
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000
  • Flow Cytometry 1 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
  • Radioimmunoassay 1:100-1:2000
  • Western Blot 2-4 ug/ml
• Application Notes: WB: Recommended dilution: 2-4 ug/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. IHC-P: Recommended dilution: 10 ug/ml; positive tissue: kidney glomeruli. Flow cytometry: Recommended dilution: 1 ug/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10-8 mol/l as determined by competitive RIA. IP: The antibody B2M-01 coprecipitates beta2-microglobulin (12 kDa) and HLA antigens (45 kDa) from the lysates of human lymphoid cells.
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  beta 2-Microglobulin Overexpression Lysate

Reactivity Notes

Weak positive staining was observed in peripheral blood lymphocytes of the monkey Cercopithecus aethiops.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for beta 2-Microglobulin Antibody (B2M-01) - BSA Free

• B2M
• beta 2Microglobulin
• beta 2-Microglobulin
• beta chain of MHC class I molecules
• beta-2-microglobin
• beta-2-microglobulin

Background

beta 2 Microglobulin non covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta 2-Microglobulin Antibody (NB500-317)(3)

Publications using NB500-317

• Rivino L, Tan AT, Chia A et al. Defining CD8+ T Cell Determinants during Human Viral Infection in Populations of Asian Ethnicity J Immunol 2013-10-15 [PMID: 24058176] (ELISA, Human)
  
  Details:
  This citation used the HRP version of this antibody.
• Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
• Tabandeh MR, Jozaie S, Ghotbedin Z, Gorani S Dimethyl itaconic acid improves viability and steroidogenesis and suppresses cytokine production in LPS-treated bovine ovarian granulosa cells by regulating TLR4/nfk beta, NLRP3, JNK signaling pathways Research in veterinary science 2022-08-01 [PMID: 35933803] (WB, Bovine)

Review for beta 2-Microglobulin Antibody (NB500-317) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Christian Schuerch IHC-P Human 03/25/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Tonsil
• Species: Human
• Lot: N/A

Comments

• Comments: pH 9 antigen retrieval

Bioinformatics

• Gene Symbol: B2M