![Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.](http://images.novusbio.com/fullsize2/beta 2-Microglobulin Antibody (B2M-01)-Western Blot-NB500-317-img0003.jpg "Western Blot: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Analysis using the FITC conjugate of NB500-317. Detection (non-reducing conditions) of whole cell lysate of various cell lines Lane 1: RAJI human Burkitt lymphoma cell line Lane 2: EL4 mouse lymphoblastic lymphoma cell line Lane 3: U937 human histiocytic lymphoma cell line.")
| Reactivity | Hu, Bv(-), Ca(-), Ch(-), Mu(-), Rb(-)Species Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, RIA |
| Clone | B2M-01 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-44521 | |||
| FITC | NB110-60015 | |||
| HRP | NBP1-44520 | |||
| Immunogen | Purified human beta 2 Microglobulin (NM_004048.2). |
| Specificity | The antibody B2M-01 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes. |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | B2M |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Purity is > 95% (by SDS-PAGE) |
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| Theoretical MW | 12 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Publications |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS (pH 7.4) |
| Preservative | 15mM Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Ammonium sulfate precipitation |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
| Publications using NB500-317 | Applications | Species |
|---|---|---|
| Rivino L, Tan AT, Chia A et al. Defining CD8+ T Cell Determinants during Human Viral Infection in Populations of Asian Ethnicity J Immunol (ELISA, Human) Details: This publication used the HRP conjugated form of this antibody (Cat# NBP1-44520). |
ELISA | Human |
| Rivino L, Tan AT, Chia A et al. Defining CD8+ T Cell Determinants during Human Viral Infection in Populations of Asian Ethnicity J Immunol 2013 Oct 15 [PMID: 24058176] (ELISA, Human) Details: This citation used the HRP version of this antibody. |
ELISA | Human |
Secondary Antibodies |
Isotype Controls |
| FITC Labeled | NB110-60015 | |
| HRP Labeled | NBP1-44520 |
Diseases for beta 2-Microglobulin Antibody (NB500-317)Discover more about diseases related to beta 2-Microglobulin Antibody (NB500-317).
| Pathways for beta 2-Microglobulin Antibody (NB500-317)View related products by pathway.
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![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Confocal microscopy of human beta2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.](http://images.novusbio.com/fullsize2/beta-2-Microglobulin-Antibody-(B2M-01)-Immunocytochemistry-Immunofluorescence-NB500-317-img0005.jpg "Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Confocal microscopy of human beta2-microglobulin-transfected canine cells labeled A) with B2M-01 antibody Alexa Fluor (R) 647 conjugate and B) with Alexa Fluor (R) 647 conjugated Mouse IgG2a Isotype Control. Cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Tween20 containing 2% BSA. Antibody concentration 2ug/ml.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PE.](http://images.novusbio.com/fullsize2/beta-2-Microglobulin-Antibody-(B2M-01)-Flow-Cytometry-NB500-317-img0011.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PE.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) FITC.](http://images.novusbio.com/fullsize2/beta-2-Microglobulin-Antibody-(B2M-01)-Flow-Cytometry-NB500-317-img0008.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) FITC.")
![Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize2/beta-2-Microglobulin-Antibody-(B2M-01)-Flow-Cytometry-NB500-317-img0010.jpg "Flow Cytometry: beta 2-Microglobulin Antibody (B2M-01) [NB500-317] - Surface staining of beta2-microglobulin in human peripheral blood with anti-beta2-microglobulin (B2M-01) PerCP-CyTM5.5.")
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - analysis of GAPDH in human nucleus pulposus cell culture (40ug/lane) using anti-GAPDH antibody. LI-COR Odyssey imaging system was used for detection. Image from verified customer review.](http://images.novusbio.com/fullsize2/GAPDH-Antibody-(1D4)-Western-Blot-NB300-221-img0005.jpg)
![Western Blot: GAPDH Antibody (1D4) [NB300-221] - Analysis of cell line lysates probed with mouse mAb to GAPDH, NB300-221, dilution 1:2,000: [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6 cells. The GAPDH antibody reveals a single band at ~37 kDa in all cell lines. GAPDH is a house keeping protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.](http://images.novusbio.com/fullsize2/GAPDH-Antibody-(1D4)-Western-Blot-NB300-221-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (1D4) [NB300-221] - SH-SY5Y cells stained with GAPDH antibody NB300-221 (green) and Neurofilament Heavy antibody NB300-217 (red), counterstained with a fluorescent DNA probe (blue).](http://images.novusbio.com/fullsize2/GAPDH-Antibody-(1D4)-Immunocytochemistry-Immunofluorescence-NB300-221-img0007.jpg)
![Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - MCDK cells induced with C22H24N2O8 to control the expression of the gene of interest. Beta actin blocking confirms the albumin assay showing that an equal amount of lysate was loaded in each lane.](http://images.novusbio.com/fullsize2/beta-Actin-Antibody-(AC-15)-Western-Blot-NB600-501-img0023.jpg)
![Western Blot: beta-Actin Antibody (AC-15) [NB600-501] - beta-Actin expression in Fibroblast Hs68 cell lysate using anti-beta-Actin antibody. Image from verified customer review.](http://images.novusbio.com/fullsize2/beta-Actin-Antibody-(AC-15)-Western-Blot-NB600-501-img0026.jpg)
![Simple Western: beta-Actin Antibody (AC-15) [NB600-501] - Analysis using the HRP conjugate of NB600-501. Simple Western lane view shows a specific band for Beta-Actin in 0.2 mg/ml of Hela, MCF-7, SH-SY5Y, & Jurkat lysate(s). This experiment was performed under reducing conditions using the 12-230kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.](http://images.novusbio.com/fullsize2/beta-Actin-Antibody-(AC-15)-Simple-Western-NB600-501-img0028.jpg)
![Immunocytochemistry/Immunofluorescence: F-Actin Antibody (NH3) [NB100-64792] - The distribution of stress fiber was observed by F-actin staining. Image from verified customer review.](http://images.novusbio.com/fullsize2/F-Actin-Antibody-(NH3)-Immunocytochemistry-Immunofluorescence-NB100-64792-img0001.jpg)
![Western Blot: HPRT Antibody [NBP1-33527] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HPRT antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/HPRT-Antibody-Western-Blot-NBP1-33527-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: HPRT Antibody [NBP1-33527] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize2/HPRT Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33527-img0002.jpg)
![Immunohistochemistry-Paraffin: HPRT Antibody [NBP1-33527] - Paraffin-embedded rat colon. HPRT antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/HPRT-Antibody-Immunohistochemistry-Paraffin-NBP1-33527-img0013.jpg)
![Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Staining of Pig Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize2/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP2-12933-img0005.jpg)
![Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - NBP2-12933 (1ug/ml) staining of HeLa nuclear (A) and cytosolic (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP2-12933-img0002.jpg)
![Western Blot: TATA binding protein TBP Antibody [NBP2-12933] - Analysis of cell line NIH3T3 (A), Mouse Testis (B) and Rat Testis (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence](http://images.novusbio.com/fullsize2/TATA-binding-protein-TBP-Antibody-Western-Blot-NBP2-12933-img0003.jpg)
![Western Blot: EIF3B Antibody [NBP2-24571] - analysis of eIF3B in A431 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using eIF3B antibody at 0.25 ug/ml, 0.25 ug/ml and 0.05 ug/ml respectively.](http://images.novusbio.com/fullsize2/EIF3B Antibody-Western Blot-NBP2-24571-img0001.jpg)
![Immunohistochemistry-Paraffin: EIF3B Antibody [NBP2-24571] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/fullsize2/EIF3B-Antibody-Immunohistochemistry-Paraffin-NBP2-24571-img0002.jpg)
![Simple Western: EIF3B Antibody [NBP2-24571] - Simple Western lane view shows a specific band for EIF3B in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/EIF3B-Antibody-Simple-Western-NBP2-24571-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:250. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31555-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - Paraffin-embedded mouse pancreas. NAGLU antibody diluted at 1:500.](http://images.novusbio.com/fullsize2/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunohistochemistry-Paraffin-NBP1-31555-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-N-acetylglucosaminidase/NAGLU Antibody [NBP1-31555] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/alpha-N-acetylglucosaminidase-NAGLU-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31555-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: NAG Antibody [NBP1-92164] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.](http://images.novusbio.com/fullsize2/NAG Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92164-img0004.jpg)
![Immunohistochemistry-Paraffin: NAG Antibody [NBP1-92164] - Staining of human heart muscle shows cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize2/NAG-Antibody-Immunohistochemistry-Paraffin-NBP1-92164-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SCN7A Antibody [NB100-81029] - Staining of sodium channel protein type 7 subunit alpha in human sperm cells.](http://images.novusbio.com/fullsize2/SCN7A-Antibody-Immunocytochemistry-Immunofluorescence-NB100-81029-img0002.jpg)
![Immunohistochemistry-Paraffin: SCN7A Antibody [NB100-81029] - Staining of normal human heart tissue formalin fixed and paraffin embedded.](http://images.novusbio.com/fullsize2/SCN7A-Antibody-Immunohistochemistry-Paraffin-NB100-81029-img0001.jpg)
![Western Blot: HMBS Antibody [NBP2-33600] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize2/HMBS-Antibody-Western-Blot-NBP2-33600-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HMBS Antibody [NBP2-33600] - Staining of human cell line A-431 shows localization to lipid droplets.](http://images.novusbio.com/fullsize2/HMBS-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33600-img0006.jpg)
![Immunohistochemistry-Paraffin: HMBS Antibody [NBP2-33600] - Staining in human bone marrow and cerebral cortex tissues using anti-HMBS antibody. Corresponding HMBS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/HMBS-Antibody-Immunohistochemistry-Paraffin-NBP2-33600-img0012.jpg)
![Western Blot: RAPGEF5 Antibody [NBP2-57362] - Analysis in human cell line RT-4, human cell line U-251 MG and human plasma.](http://images.novusbio.com/fullsize2/RAPGEF5 Antibody-Western Blot-NBP2-57362-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: RAPGEF5 Antibody [NBP2-57362] - Staining of human cell line PC-3 shows localization to nucleoplasm & nuclear bodies.](http://images.novusbio.com/fullsize2/RAPGEF5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57362-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MHC Class I Antibody (OX18) [NB120-6405] - PC-12 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-MHC Class I (OX18) NB120-6405 at a 1:100 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/MHC-Class-I-Antibody-(OX18)-Immunocytochemistry-Immunofluorescence-NB120-6405-img0003.jpg)
![Immunohistochemistry-Paraffin: MHC Class I Antibody (OX18) [NB120-6405] - IHC analysis of formalin fixed paraffin-embedded (FFPE) rat brain cerebellum using MHC Class I (OK18) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Endothelial staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize2/MHC-Class-I-Antibody-(OX18)-Immunohistochemistry-Paraffin-NB120-6405-img0008.jpg)
![Flow Cytometry: MHC Class I Antibody (OX18) [NB120-6405] - Analysis using the FITC conjugate of NB120-6405. Staining of rat spleen cells with mouse anti-rat RT1-A (OX18).](http://images.novusbio.com/fullsize2/MHC-Class-I-Antibody-(OX18)-Flow-Cytometry-NB120-6405-img0007.jpg)
