POMC Antibody


Western Blot: POMC Antibody [NB100-1533] - Staining of NIH3T3 cell lysate with antibody at 0.3 ug/mL (A) and Rat Brain cell lysate with antibody at 2 ug/mL (B). 35 ug protein in RIPA buffer. Detected by ...read more
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows ...read more
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped ...read more

Product Details

Reactivity Hu, Mu, Rt, Po, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
0.5 mg/ml

Order Details

POMC Antibody Summary

Peptide with sequence C-NAIIKNAYKKGE corresponding to C-Terminus according to NP_000930.1, NP_001030333.1.
C Terminus
Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 0.3 - 2 ug/mL
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 2 - 4 ug/mL
  • Immunohistochemistry-Paraffin 2 - 4 ug/mL
  • Peptide ELISA 1:128000
Application Notes
ICC/IF reactivity reported in the literature (PMID: 30709906). Western blot: Approx 27 kDa band observed in lysates of cell line NIH3T3 and approx 30 kDa in Rat Brain lysates (calculated MW of 26.7 kDa according to Mouse NP_001265510.1 1 and 26.6 kDa according to Rat NP_647542.1).
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
NIH 3T3 Whole Cell Lysate
POMC Overexpression Lysate
Control Peptide
POMC Peptide (NB100-1533PEP)
Read Publications using
NB100-1533 in the following applications:

  • 2 publications
  • IHC
    2 publications
  • WB
    6 publications

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 28250970). Sheep reactivity reported in scientific literature (PMID:15661821). Rat reactivity reported in scientific literature (PMID: 23358747).

Packaging, Storage & Formulations

Store at -20C. Avoid freeze-thaw cycles.
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
0.02% Sodium Azide
0.5 mg/ml
Immunogen affinity purified

Alternate Names for POMC Antibody

  • ACTH
  • adrenocorticotropic hormone
  • adrenocorticotropin
  • alpha-melanocyte-stimulating hormone
  • alpha-MSH
  • beta-endorphin
  • beta-LPH
  • beta-melanocyte-stimulating hormone
  • beta-MSH
  • CLIP
  • corticotropin-like intermediary peptide
  • corticotropin-lipotropin
  • gamma-LPH
  • gamma-MSH
  • lipotropin beta
  • lipotropin gamma
  • LPH
  • melanotropin alpha
  • melanotropin beta
  • melanotropin gamma
  • met-enkephalin
  • MSH
  • NPP
  • POC
  • POMC
  • pro-ACTH-endorphin
  • proopiomelanocortin preproprotein
  • proopiomelanocortin
  • pro-opiomelanocortin


This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for POMC Antibody (NB100-1533)(10)

We have publications tested in 4 confirmed species: Mouse, Rat, Porcine, Sheep.

We have publications tested in 3 applications: ICC/IF, IHC, WB.

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Showing Publications 1 - 10 of 10.
Publications using NB100-1533 Applications Species
Wang Z, Khor S, Cai D. Age-dependent decline of hypothalamic HIF2a in response to insulin and its contribution to advanced age-associated metabolic disorders in mice J. Biol. Chem. Feb 1 2019 [PMID: 30709906] (ICC/IF, Mouse) ICC/IF Mouse
Mandal SK, Briski KP. Hindbrain Dorsal Vagal Complex AMPK Controls Hypothalamic Gluco-regulatory Transmitter and Counter-Regulatory Hormone Responses to Hypoglycemia. Brain Res. Bull. Nov 24 2018 [PMID: 30481553] (WB, Rat) WB Rat
Shakya M, Shrestha PK, Briski KP et al. Hindbrain 5'-adenosine monophosphate-activated protein kinase Mediates Short-Term Food Deprivation Inhibition of the Gonadotropin-Releasing Hormone-Luteinizing Hormone Axis: Role of Nitric Oxide Neuroscience. 2018 May 06 [PMID: 29746990] (WB, Rat) WB Rat
Kim GH, Shi G, Somlo DR, Haataja L. Hypothalamic ER-associated degradation regulates POMC maturation, feeding, and age-associated obesity. J. Clin. Invest. 2018 Feb 19 [PMID: 29457782] (IHC, Mouse) IHC Mouse
Klasvogt S, Zuschratter W, Schmidt A et al. Air-liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2. Cell Death Discov. 2017 Mar 02 [PMID: 28250970] (WB, Porcine) WB Porcine
Trebak F, Dubuc I, Arabo A et al. A Potential Role for the Secretogranin II-Derived Peptide EM66 in the Hypothalamic Regulation of Feeding Behavior. J. Neuroendocrinol. 2017 Feb 06 [PMID: 28166374]
Evans JF, Rodriguez S, Ragolia L. ACTH promotes chondrogenic nodule formation and induces transient elevations in intracellular calcium in rat bone marrow cell cultures via MC2-R signaling. Cell Tissue Res 2013 Jan 29 [PMID: 23358747] (WB, Rat) WB Rat
Evans JF, Fernando A, Ragolia. Functional melanocortin-2 receptors are expressed by mouse aorta-derived mesenchymal progenitor cells Mol Cell Endocrinol 2012 May 15 [PMID: 22306084] (WB, Mouse) WB Mouse
Kaushik S, Arias E, Kwon H et al. Loss of autophagy in hypothalamic POMC neurons impairs lipolysis EMBO Rep 2012 Mar [PMID: 22249165] (WB, IHC, ICC/IF, Mouse) WB, IHC, ICC/IF Mouse
Muhlhausler B, Adam C, Marrocco E et al. Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation. J Physiol. 2005 May 15 [PMID: 15661821] (Sheep) Sheep

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Product General Protocols

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ICC/IF Video Protocol

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Gene Symbol POMC