Western Blot: POMC Antibody [NB100-1533] - Western blot analysis of POMC using NB100-1533 at 0.01ug/ml in NIH 3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Analysis in human Anterior Pituitary Gland shows vesilulate staining in glandular cells.
WB: Approx. 29 kDa band observed in NIH3T3 lysates (calculated MW of 29.4 kDa band according to NP_000930.1 and NP_001030333.1). IHC-P: Human Anterior Pituitary gland shows vesilulate staining in glandular cells. ICC/IF reactivity reported in scientific literature (PMID: 22249165).
29.4 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Predicted cross-reactivity based on sequence identity: Rat. Porcine reactivity reported in scientific literature (PMID: 28250970). Sheep reactivity reported in scientific literature (PMID:15661821).
Packaging, Storage & Formulations
Store at -20C. Avoid freeze-thaw cycles.
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for POMC Antibody
corticotropin-like intermediary peptide
This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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