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POMC Antibody

Images

 
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative confocal images of POMC in POMC-transfected WT and Sel1L-/- N2a cells. White arrows point to POMC-containing secretory granules, while yellow arrows ...read more
Flow Cytometry: POMC Antibody [NB100-1533] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 ...read more
Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Staining of paraffin embedded Human Pituitary Gland. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunohistochemistry: POMC Antibody [NB100-1533] - Representative immunofluorescence images of (B) POMC as a prohormone (n = 5-6 each) in the ARC of mice approximately 5 to 10 weeks of age. Image collected and cropped ...read more
p27S10 phosphorylation is required for PHD3 depletion induced increase in p27 half-life. a Quantification of p27S10 phosphorylation from three independent experiments in 786-O cells. S10 phosphorylation demonstrated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ShSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

POMC Antibody Summary

Immunogen
Peptide with sequence C-NAIIKNAYKKGE corresponding to C-Terminus according to NP_000930.1, NP_001030333.1.
Epitope
C Terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POMC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/mL
  • Peptide ELISA 1:128000
  • Western Blot
Application Notes
ICC/IF reactivity reported in the literature (PMID: 30709906). Use in WB reported in the literature (PMID: 22249165). WB: Preliminary testing showed a band at approx 30kDa in A431 cell lysate after 1ug/ml antibody staining (calculated MW of 29.4kDa according to NP_000930.1). Primary incubation 1 hour at room temperature.
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Whole Cell Lysate
POMC Overexpression Lysate
Control Peptide
POMC Peptide (NB100-1533PEP)
Publications
Read Publications using
NB100-1533 in the following applications:

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 28250970). Sheep reactivity reported in scientific literature (PMID:15661821). Rat reactivity reported in scientific literature (PMID: 23358747).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POMC Antibody

  • ACTH
  • adrenocorticotropic hormone
  • adrenocorticotropin
  • alpha-melanocyte-stimulating hormone
  • alpha-MSH
  • beta-endorphin
  • beta-LPH
  • beta-melanocyte-stimulating hormone
  • beta-MSH
  • CLIP
  • corticotropin-like intermediary peptide
  • corticotropin-lipotropin
  • gamma-LPH
  • gamma-MSH
  • lipotropin beta
  • lipotropin gamma
  • LPH
  • melanotropin alpha
  • melanotropin beta
  • melanotropin gamma
  • met-enkephalin
  • MSH
  • NPP
  • POC
  • POMC
  • pro-ACTH-endorphin
  • proopiomelanocortin preproprotein
  • proopiomelanocortin
  • pro-opiomelanocortin

Background

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1533
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, ELISA, Flow, ICC/IF, IHC

Publications for POMC Antibody (NB100-1533)(10)

We have publications tested in 4 confirmed species: Mouse, Rat, Porcine, Sheep.

We have publications tested in 3 applications: ICC/IF, IF/IHC, WB.


Filter By Application
ICC/IF
(2)
IF/IHC
(2)
WB
(6)
All Applications
Filter By Species
Mouse
(4)
Rat
(3)
Porcine
(1)
Sheep
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-1533 Applications Species
Wang Z, Khor S, Cai D. Age-dependent decline of hypothalamic HIF2a in response to insulin and its contribution to advanced age-associated metabolic disorders in mice J. Biol. Chem. 2019-02-01 [PMID: 30709906] (ICC/IF, Mouse) ICC/IF Mouse
Mandal SK, Briski KP. Hindbrain Dorsal Vagal Complex AMPK Controls Hypothalamic Gluco-regulatory Transmitter and Counter-Regulatory Hormone Responses to Hypoglycemia. Brain Res. Bull. 2018-11-24 [PMID: 30481553] (WB, Rat) WB Rat
Shakya M, Shrestha PK, Briski KP et al. Hindbrain 5'-adenosine monophosphate-activated protein kinase Mediates Short-Term Food Deprivation Inhibition of the Gonadotropin-Releasing Hormone-Luteinizing Hormone Axis: Role of Nitric Oxide Neuroscience. 2018-05-06 [PMID: 29746990] (WB, Rat) WB Rat
Kim GH, Shi G, Somlo DR, Haataja L. Hypothalamic ER-associated degradation regulates POMC maturation, feeding, and age-associated obesity. J. Clin. Invest. 2018-02-19 [PMID: 29457782] (IF/IHC, Mouse) IF/IHC Mouse
Klasvogt S, Zuschratter W, Schmidt A et al. Air-liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2. Cell Death Discov. 2017-03-02 [PMID: 28250970] (WB, Porcine) WB Porcine
Trebak F, Dubuc I, Arabo A et al. A Potential Role for the Secretogranin II-Derived Peptide EM66 in the Hypothalamic Regulation of Feeding Behavior. J. Neuroendocrinol. 2017-02-06 [PMID: 28166374]
Evans JF, Rodriguez S, Ragolia L. ACTH promotes chondrogenic nodule formation and induces transient elevations in intracellular calcium in rat bone marrow cell cultures via MC2-R signaling. Cell Tissue Res 2013-01-29 [PMID: 23358747] (WB, Rat) WB Rat
Evans JF, Fernando A, Ragolia. Functional melanocortin-2 receptors are expressed by mouse aorta-derived mesenchymal progenitor cells Mol Cell Endocrinol 2012-05-15 [PMID: 22306084] (WB, Mouse) WB Mouse
Kaushik S, Arias E, Kwon H et al. Loss of autophagy in hypothalamic POMC neurons impairs lipolysis EMBO Rep 2012-03-01 [PMID: 22249165] (ICC/IF, IF/IHC, WB, Mouse) ICC/IF, IF/IHC, WB Mouse
Muhlhausler B, Adam C, Marrocco E et al. Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation. J Physiol. 2005-05-15 [PMID: 15661821] (Sheep) Sheep

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol POMC
Entrez
Uniprot