POMC Antibody

Images

 
Western Blot: POMC Antibody [NB100-1533] - Western blot analysis of POMC using NB100-1533 at 0.01ug/ml in NIH 3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: POMC Antibody [NB100-1533] - Analysis in human Anterior Pituitary Gland shows vesilulate staining in glandular cells.

Product Details

Summary
Reactivity Hu, Mu, Po, ShSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

POMC Antibody Summary

Immunogen
Peptide with sequence C-NAIIKNAYKKGE corresponding to C-Terminus according to NP_000930.1, NP_001030333.1.
Epitope
C Terminus
Specificity
Both transcript variants encode the same protein. In Western blot, this product appears to react with the full length precursor protein only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POMC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 03 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 2.4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA 1:128000
Application Notes
IHC-P: Human Anterior Pituitary gland shows vesilulate staining in glandular cells. ICC/IF reactivity reported in scientific literature (PMID: 22249165).
Theoretical MW
29.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
NIH 3T3 Lysate (NB800-PC8)
POMC Lysate (NBL1-14599)
Control Peptide
POMC Peptide (NB100-1533PEP)
Publications
Read Publications using
NB100-1533 in the following applications:

  • 1 publication
  • IHC
    2 publications
  • WB
    4 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat. Porcine reactivity reported in scientific literature (PMID: 28250970). Sheep reactivity reported in scientific literature (PMID:15661821).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for POMC Antibody

  • ACTH
  • adrenocorticotropic hormone
  • adrenocorticotropin
  • alpha-melanocyte-stimulating hormone
  • alpha-MSH
  • beta-endorphin
  • beta-LPH
  • beta-melanocyte-stimulating hormone
  • beta-MSH
  • CLIP
  • corticotropin-like intermediary peptide
  • Corticotropin-lipotropin
  • gamma-LPH
  • gamma-MSH
  • lipotropin beta
  • lipotropin gamma
  • LPH
  • melanotropin alpha
  • melanotropin beta
  • melanotropin gamma
  • met-enkephalin
  • MSH
  • NPP
  • POC
  • POMC
  • pro-ACTH-endorphin
  • proopiomelanocortin preproprotein
  • proopiomelanocortin
  • pro-opiomelanocortin

Background

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for POMC Antibody (NB100-1533)(7)

We have publications tested in 4 confirmed species: Mouse, Rat, Porcine, Sheep.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(2)
WB
(4)
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Filter By Species
Mouse
(3)
Rat
(1)
Porcine
(1)
Sheep
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-1533 Applications Species
Kim GH, Shi G, Somlo DR, Haataja L. Hypothalamic ER-associated degradation regulates POMC maturation, feeding, and age-associated obesity. J. Clin. Invest. 2018 Feb 19 [PMID: 29457782] (IHC, Mouse) IHC Mouse
Klasvogt S, Zuschratter W, Schmidt A et al. Air-liquid interface enhances oxidative phosphorylation in intestinal epithelial cell line IPEC-J2. Cell Death Discov. 2017 Mar 02 [PMID: 28250970] (WB, Porcine) WB Porcine
Trebak F, Dubuc I, Arabo A et al. A Potential Role for the Secretogranin II-Derived Peptide EM66 in the Hypothalamic Regulation of Feeding Behavior. J. Neuroendocrinol. 2017 Feb 06 [PMID: 28166374]
Evans JF, Rodriguez S, Ragolia L. ACTH promotes chondrogenic nodule formation and induces transient elevations in intracellular calcium in rat bone marrow cell cultures via MC2-R signaling. Cell Tissue Res 2013 Jan 29 [PMID: 23358747] (WB, Rat) WB Rat
Evans JF, Fernando A, Ragolia. Functional melanocortin-2 receptors are expressed by mouse aorta-derived mesenchymal progenitor cells Mol Cell Endocrinol 2012 May 15 [PMID: 22306084] (WB, Mouse) WB Mouse
Kaushik S, Arias E, Kwon H et al. Loss of autophagy in hypothalamic POMC neurons impairs lipolysis EMBO Rep 2012 Mar [PMID: 22249165] (WB, IHC, ICC/IF, Mouse) WB, IHC, ICC/IF Mouse
Muhlhausler B, Adam C, Marrocco E et al. Impact of glucose infusion on the structural and functional characteristics of adipose tissue and on hypothalamic gene expression for appetite regulatory neuropeptides in the sheep fetus during late gestation. J Physiol. 2005 May 15 [PMID: 15661821] (Sheep) Sheep

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol POMC
Entrez
Uniprot