Complement C3 Antibody (11H9)

Images

 
Biological Strategies: Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a ...read more
Immunocytochemistry/ Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in ...read more
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but ...read more
Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow ...read more
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, Flow-IC, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
Clone
11H9
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Complement C3 Antibody (11H9) Summary

Immunogen
This Complement C3 Antibody (11H9) was developed against C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1).
Localization
Secreted
Specificity
Mouse Complement C3 and its activation products, C3b, iC3b, C3d and C3dg
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
C3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/mL
  • Immunoassay 0.5 ug/well in PBS
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
  • Flow (Intracellular)
Theoretical MW
187 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB200-540 in the following applications:

Publications
Read Publications using
NB200-540 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Complement C3 Antibody (11H9)

  • Acylation Stimulating Protein
  • acylation-stimulating protein cleavage product
  • AHUS5
  • ARMD9
  • ASP
  • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
  • C3
  • C3a anaphylatoxin
  • C3a
  • C3adesArg
  • C3b
  • C3bc
  • C3-beta-c
  • Complement C3 alpha chain
  • Complement C3 beta chain
  • complement C3
  • Complement C3b alpha' chain
  • Complement C3c alpha' chain fragment 1
  • Complement C3c alpha' chain fragment 2
  • Complement C3d fragment
  • Complement C3dg fragment
  • Complement C3f fragment
  • Complement C3g fragment
  • complement component 3
  • complement component C3
  • complement component C3a
  • complement component C3b
  • CPAMD1
  • EC 3.4.21.43
  • epididymis secretory sperm binding protein Li 62p
  • HEL-S-62p
  • prepro-C3

Background

The complement system, or complement cascade, is a part of the innate immune system that assists in defense against pathogens (1-3). Complement C3, also called C3 or C3 protein, is one of nine complement proteins and is the main component of the complement system which is composed of over 30 soluble and membrane-bound proteins (1,4). The complement cascade consists of three main pathways: the classical, the lectin, and the alternative, all of which converge into a common pathway involving C3 cleavage by C3-convertases (1-6). Human Complement C3 is synthesized as a protein of 1663 amino acids (aa) in length with a theoretical molecular weight of ~185 kDa (5). Complement C3 is the most prevalent human complement protein in the serum, with a concentration of 1.2 mg/mL, and is predominantly produced by hepatocytes in the liver, but is also synthesized by blood cells and epithelial cells (3,5). Furthermore, the structure of C3 is comprised of an alpha-chain (110 kDa) and a beta-chain (75 kDa) linked by a disulfide bond (5). Cleavage of inactive C3 by C3-convertases produces active C3a, which functions as a mediator of inflammation, and C3b, which is an opsonin (1-4). In addition to amplification of complement response, C3 fragments serve multiple additional functions including chemotaxis, phagocytosis, adhesion, and immune modulation (3). Complement C3 serves dual purposes where it is involved in pathogenesis and immunity but, conversely, cellular damage results from unregulated C3 activation (5).

Both elevated levels and reduced levels of Complement C3 has been implicated in diseases pathologies (6). Deficiency in Complement proteins can result in autoimmune disorders including systemic lupus erythematosus, which is more often associated with C1 or C4 deficiency and only rarely with C3 deficiency (6). However, C3 deficiency typically results in increased risk of recurrent bacterial infections and glomerulonephritis, characterized by inflammation of the filtering glomeruli in the kidneys (6). Additionally, elevated levels of C3a and C4a is seen in patients with antiphospholipid antibody syndrome (6). Serum levels of C3 are also higher in rheumatoid arthritis cases (6). The complement system has become a target for drugs and therapeutics aimed at modulating innate immunity (7). For instance, compstatin is a peptide that binds to C3, inhibiting convertase activity and cleavage and can be used to treat diseases associated with uncontrolled C3 activation (7). C3-inhibitors and other complement inhibitors are a promising drug candidate for treatment of many diseases (7).

References

1. Mathern, D. R., & Heeger, P. S. (2015). Molecules Great and Small: The Complement System. Clinical Journal of the American Society of Nephrology: CJASN. https://doi.org/10.2215/CJN.06230614

2. Merle, N. S., Church, S. E., Fremeaux-Bacchi, V., & Roumenina, L. T. (2015). Complement System Part I - Molecular Mechanisms of Activation and Regulation. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2015.00262

3. Ricklin, D., Reis, E. S., Mastellos, D. C., Gros, P., & Lambris, J. D. (2016). Complement component C3 - The "Swiss Army Knife" of innate immunity and host defense. Immunological Reviews. https://doi.org/10.1111/imr.12500

4. Merle, N. S., Noe, R., Halbwachs-Mecarelli, L., Fremeaux-Bacchi, V., & Roumenina, L. T. (2015). Complement System Part II: Role in Immunity. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2015.00257

5. Sahu, A., & Lambris, J. D. (2001). Structure and biology of complement protein C3, a connecting link between innate and acquired immunity. Immunological Reviews. https://doi.org/10.1034/j.1600-065x.2001.1800103.x

6. Vignesh, P., Rawat, A., Sharma, M., & Singh, S. (2017). Complement in autoimmune diseases. Clinica Chimica Acta; International Journal of Clinical Chemistry. https://doi.org/10.1016/j.cca.2016.12.017

7. Mastellos, D. C., Yancopoulou, D., Kokkinos, P., Huber-Lang, M., Hajishengallis, G., Biglarnia, A. R., Lupu, F., Nilsson, B., Risitano, A. M., Ricklin, D., & Lambris, J. D. (2015). Compstatin: a C3-targeted complement inhibitor reaching its prime for bedside intervention. European Journal of Clinical Investigation. https://doi.org/10.1111/eci.12419

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-67656
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-88527
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-93935
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89985
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
AF4779
Species: Hu
Applications: WB, IHC
NBP1-87492
Species: Hu
Applications: WB, IHC, IHC-P
NB600-41532
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
H00005688-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
NB120-11899
Species: Mu
Applications: WB, IHC, IHC-Fr
NBP1-85466
Species: Hu, Mu, Fe
Applications: WB, IHC, IHC-P
AF8216
Species: Hu, Mu, Rt
Applications: WB
NB100-66539
Species: Hu, RM
Applications: WB, Flow, IHC, IHC-Fr, IHC-P
NB200-540
Species: Mu
Applications: Flow, Flow-IC, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready

Publications for Complement C3 Antibody (NB200-540)(11)

We have publications tested in 2 confirmed species: Mouse, E. coli.

We have publications tested in 5 applications: ELISA, Flow, ICC/IF, IHC, IP.


Filter By Application
ELISA
(1)
Flow
(1)
ICC/IF
(2)
IHC
(5)
IP
(1)
All Applications
Filter By Species
Mouse
(9)
E. coli
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NB200-540 Applications Species
Griffin P, Sheehan PW, Dimitry JM et al. REV-ERB alpha mediates complement expression and diurnal regulation of microglial synaptic phagocytosis eLife Dec 1 2020 [PMID: 33258449] (IHC, Mouse) IHC Mouse
Davidson S, Efremova M, Riedel A et al. Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth Cell Rep May 19 2020 [PMID: 32433953] (ICC/IF, Mouse) ICC/IF Mouse
Holland-Tummillo KM, Shoudy LE, Steiner D et al. Autotransporter-Mediated Display of Complement Receptor Ligands by Gram-Negative Bacteria Increases Antibody Responses and Limits Disease Severity Pathogens May 14 2020 [PMID: 32422907] (IP, E. coli) IP E. coli
Sun D, Sun P, He S, Shi M Rat IgG mediated circulatory cell depletion in mice requires mononuclear phagocyte system and is facilitated by complement J. Leukoc. Biol. Jan 22 2020 [PMID: 31965640] (Flow, Mouse) Flow Mouse
Wang K Wei H Zhan J et al. GSPE alleviates renal fibrosis by inhibiting the activation of C3/ HMGB1/ TGF- beta 1 pathway Chem. Biol. Interact. Dec 23 2019 [PMID: 31874164] (ICC/IF, Mouse) ICC/IF Mouse
Campbell SC, MuNoz-Ballester C, Chaunsali L, et al. Potassium and glutamate transport is impaired in scar-forming tumor-associated astrocytes Neurochem. Int. Dec 9 2019 [PMID: 31825815] (IHC, Mouse) IHC Mouse
Medler TR, Murugan D, Horton W et al. Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy. Cancer Cell. Oct 8 2018 [PMID: 30300579] (IHC, Mouse) IHC Mouse
Davidson S Defining the pro-tumour impact of the evolving stromal microenvironment Thesis Jan 1 2020
Wu T, Dejanovic B, Gandham VD et al. Complement C3 Is Activated in Human AD Brain and Is Required for Neurodegeneration in Mouse Models of Amyloidosis and Tauopathy Cell Rep Aug 20 2019 [PMID: 31433986] (IHC, Mouse) IHC Mouse
Chakraborty S Complement factors in murine jejunum mucus and their functional alteration after experimental sepsis Thesis Jan 1 2021 (ELISA, Mouse) ELISA Mouse
Show All 11 Publications.

Reviews for Complement C3 Antibody (NB200-540) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NB200-540:
Filter by Applications
Flow
(1)
ICC
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry Complement C3 NB200-540
Enlarge
5
reviewed by:
Francesca Azar
Flow Mouse 05/16/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedBreast tumor
SpeciesMouse
LotAB042114A-06

Comments

CommentsDespite some issues with our secondary antibody, this antibody worked well for flow cytometry analysis of mouse tumor tissue.
  4
reviewed by:
Anonymous
ICC Mouse 04/01/2017
View

Summary

ApplicationImmunocytochemistry
Sample Tested brain and spinal cord
SpeciesMouse

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for Complement C3 Antibody (NB200-540). (Showing 1 - 1 of 1 FAQs).

  1. I am trying to establish a method to measure mice C3 levels by nephelometry. I would be most grateful if you could provide me with some help, regarding the choice of the Ab. I totally understand that since such a method has never been tried, I do not expect any guaranties. 
    • We have never performed nephelometry in our lab, and do not have a protocol or advice to provide about this application. However, it seems to me that you should choose an antibody that is capable of recognizing its target in its folded conformation. Therefore, I would suggest trying an antibody that has been validated for ICC or IHC.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB200-540AF350
Alexa Fluor 405 NB200-540AF405
Alexa Fluor 488 NB200-540AF488
Alexa Fluor 532 NB200-540AF532
Alexa Fluor 594 NB200-540AF594
Alexa Fluor 647 NB200-540AF647
Alexa Fluor 700 NB200-540AF700
Alexa Fluor 750 NB200-540AF750
Allophycocyanin NB200-540APC
Biotin NB200-540B
DyLight 350 NB200-540UV
DyLight 405 NB200-540V
DyLight 488 NB200-540G
DyLight 550 NB200-540R
DyLight 594 NB200-540DL594
DyLight 650 NB200-540C
DyLight 680 NB200-540FR
DyLight 755 NB200-540IR
FITC NB200-540F
HRP NB200-540H
Janelia Fluor 549 NB200-540JF549
Janelia Fluor 646 NB200-540JF646
PE NB200-540PE
PerCP NB200-540PCP

Additional Complement C3 Products

Bioinformatics Tool for Complement C3 Antibody (NB200-540)

Discover related pathways, diseases and genes to Complement C3 Antibody (NB200-540). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Complement C3 Antibody (NB200-540)

Discover more about diseases related to Complement C3 Antibody (NB200-540).
 

Pathways for Complement C3 Antibody (NB200-540)

View related products by pathway.

PTMs for Complement C3 Antibody (NB200-540)

Learn more about PTMs related to Complement C3 Antibody (NB200-540).
 

Research Areas for Complement C3 Antibody (NB200-540)

Find related products by research area.

Blogs on Complement C3.

Complement C3 - the most important protein in the complement system
The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Francesca Azar
05/16/2019
Application: Flow
Species: Mouse

 
Anonymous
04/01/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol C3
Entrez
Uniprot