Complement C3 Antibody (11H9)

Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&D Systems).

Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.

Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.

Flow Cytometry: Complement C3 Antibody (11H9) [NB200-540] - Left panel: FMO, Middle panel: No primary antibody control, Right panel: sample. Day 6 murine mammary tumors processed and stained for analysis with flow cytometry. The C3b+ population of CD45+ cells is what the gate in each sample is exhibiting. Image from verified customer review.

Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse lymph node using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. This representative photomicrograph shows a membrane-cytoplasmic immunopositivity in non-germinal center cells, and few cells developed an intense staining for this target protein.

• Reactivity: Mu
• Applications: Flow, Flow-IC, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready
• Clone: 11H9
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Complement C3 Antibody (11H9) Summary

• Immunogen: C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1)
• Localization: Secreted
• Specificity: Mouse Complement C3 and its activation products, C3b, iC3b, C3d and C3dg
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: C3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/mL
  • Immunoassay 0.5 ug/well in PBS
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • CyTOF-ready
  • Flow (Intracellular)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Complement C3 Antibody (11H9)

• AHUS5
• ARMD9
• ASP
• C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
• complement C3
• complement component 3
• complement component C3
• CPAMD1acylation-stimulating protein cleavage product

Background

Complement component 3, or C3, is one of nine major complement proteins in the complement system. This system is vital to the immune system because of its role in killing unwanted bacteria and its part in the initiation of inflammation. The complement system is made up of a collection of proteins found in the bloodstream. C3 happens to be the most important protein as well as the most plentiful among the other proteins in the complement system. Levels of C3 in the blood are often tested in patients with autoimmune disorders to monitor the effects of their treatment. A C3 deficiency can cause increased risk for bacterial infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement C3 Antibody (NB200-540)(8)

Publications using NB200-540

• Davidson S, Efremova M, Riedel A et al. Single-Cell RNA Sequencing Reveals a Dynamic Stromal Niche That Supports Tumor Growth Cell Rep May 19 2020 [PMID: 32433953] (ICC/IF, Mouse)
• Holland-Tummillo KM, Shoudy LE, Steiner D et al. Autotransporter-Mediated Display of Complement Receptor Ligands by Gram-Negative Bacteria Increases Antibody Responses and Limits Disease Severity Pathogens May 14 2020 [PMID: 32422907] (IP, E. coli)
• Sun D, Sun P, He S, Shi M Rat IgG mediated circulatory cell depletion in mice requires mononuclear phagocyte system and is facilitated by complement J. Leukoc. Biol. Jan 22 2020 [PMID: 31965640] (Flow, Mouse)
• Wang K Wei H Zhan J et al. GSPE alleviates renal fibrosis by inhibiting the activation of C3/ HMGB1/ TGF- beta 1 pathway Chem. Biol. Interact. Dec 23 2019 [PMID: 31874164] (ICC/IF, Mouse)
• Campbell SC, MuNoz-Ballester C, Chaunsali L, et al. Potassium and glutamate transport is impaired in scar-forming tumor-associated astrocytes Neurochem. Int. Dec 9 2019 [PMID: 31825815] (IHC, Mouse)
• Wu T, Dejanovic B, Gandham VD et al. Complement C3 Is Activated in Human AD Brain and Is Required for Neurodegeneration in Mouse Models of Amyloidosis and Tauopathy Cell Rep Aug 20 2019 [PMID: 31433986] (IHC, Mouse)
• Medler TR, Murugan D, Horton W et al. Complement C5a Fosters Squamous Carcinogenesis and Limits T Cell Response to Chemotherapy. Cancer Cell. Oct 8 2018 [PMID: 30300579] (IHC, Mouse)
• Maehara N, Arai S, Mori M et al. Circulating AIM Prevents Hepatocellular Carcinoma through Complement Activation. Cell Rep. 2014 Oct 09 [PMID: 25284781] (IHC, Mouse)

Reviews for Complement C3 Antibody (NB200-540) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Francesca Azar Flow Mouse 05/16/2019

Summary

• Application: Flow Cytometry
• Sample Tested: Breast tumor
• Species: Mouse
• Lot: AB042114A-06

Comments

• Comments: Despite some issues with our secondary antibody, this antibody worked well for flow cytometry analysis of mouse tumor tissue.

reviewed by: Anonymous ICC Mouse 04/01/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: brain and spinal cord
• Species: Mouse

FAQs for Complement C3 Antibody (NB200-540). (Showing 1 - 1 of 1 FAQs).

1. I am trying to establish a method to measure mice C3 levels by nephelometry. I would be most grateful if you could provide me with some help, regarding the choice of the Ab. I totally understand that since such a method has never been tried, I do not expect any guaranties. 
   • We have never performed nephelometry in our lab, and do not have a protocol or advice to provide about this application. However, it seems to me that you should choose an antibody that is capable of recognizing its target in its folded conformation. Therefore, I would suggest trying an antibody that has been validated for ICC or IHC.

Bioinformatics

• Gene Symbol: C3
• Entrez:
  • 12266(Mouse)
• Uniprot:
  • P01027()