Reactivity | MuSpecies Glossary |
Applications | WB, Flow, Flow-IC, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready |
Clone | 11H9 |
Clonality | Monoclonal |
Host | Rat |
Conjugate | Unconjugated |
Concentration | 1.0 mg/ml |
Biological Strategies
Immunogen | C57BL/6 thymocytes saturated with rat anti-Thy-1 monoclonal antibody of IgG2b subclass (RmT1) |
Localization | Secreted |
Specificity | Mouse Complement C3 and its activation products, C3b, iC3b, C3d and C3dg |
Isotype | IgG2a |
Clonality | Monoclonal |
Host | Rat |
Gene | C3 |
Purity | Protein G purified |
Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
Dilutions |
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Application Notes | For IHC-F: Tissue sections were fixed in acetone. Antigen retrieval was performed using 50% formic acid for 5 min or microwaved (700 W) in antigen unmasking solution. For IHC-P: free-floating brain tissue pretreated with 50% formic acid for 5 min. or microwaved (700W) in antigen unmasking solution for 2-5min. Blocking endogenous persoxidase by treatment with 3% H2O2 in pBS 20 min at 25 degrees C. Blocking 2hrs, 2% BSA in PBS. For IF: Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval. |
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Reviewed Applications |
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Publications |
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Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | PBS |
Preservative | 0.05% Sodium Azide |
Concentration | 1.0 mg/ml |
Purity | Protein G purified |
Publication using NB200-540 | Applications | Species |
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Maehara N, Arai S, Mori M et al. Circulating AIM Prevents Hepatocellular Carcinoma through Complement Activation. Cell Rep. 2014 Oct 09 [PMID: 25284781] (IHC, Mouse) | IHC | Mouse |
Images | Ratings | Applications | Species | Date | Details | ||||||
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reviewed by:
Anonymous |
ICC | Mouse | 04/01/2017 |
Summary
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Secondary Antibodies |
Isotype Controls |
Diseases for Complement C3 Antibody (NB200-540)Discover more about diseases related to Complement C3 Antibody (NB200-540).
| Pathways for Complement C3 Antibody (NB200-540)View related products by pathway.
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Complement C3 - the most important protein in the complement system The complement system is made up of a collection of proteins found in the bloodstream and is comprised of nine major complement proteins; complement C3 is one of them. The complement system is a crucial component of the cellular immune system becau... Read full blog post. |
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