Insulin Antibody (182410) [Unconjugated]

Images

 
Insulin was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications IHC, CyTOF-ready, Flow, ICC/IF
Clone
182410
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Insulin Antibody (182410) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Insulin
Specificity
Detects bovine, human, and mouse insulin.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
INS
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.5-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Publications
Read Publications using
MAB1417 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Insulin Antibody (182410) [Unconjugated]

  • IDDM2
  • ILPR
  • INS
  • Insulin
  • IRDN
  • MODY10
  • proinsulin

Background

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Insulin Antibody (MAB1417)(14)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: Flow Cytometry, ICC, IHC, IHC-P.


Filter By Application
Flow Cytometry
(4)
ICC
(4)
IHC
(4)
IHC-P
(2)
All Applications
Filter By Species
Mouse
(14)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using MAB1417 Applications Species
L Lei, Y Huan, Q Liu, C Li, H Cao, W Ji, X Gao, Y Fu, P Li, R Zhang, Z Abliz, Y Liu, S Liu, Z Shen Morus alba L. (Sangzhi) Alkaloids Promote Insulin Secretion, Restore Diabetic beta-Cell Function by Preventing Dedifferentiation and Apoptosis Frontiers in Pharmacology, 2022;13(0):841981. 2022 [PMID: 35308210] (IHC, Mouse) IHC Mouse
MR Brown, SK Sen, A Mazzone, TK Her, Y Xiong, JH Lee, N Javeed, CS Colwell, K Rakshit, NK LeBrasseur, A Gaspar-Mai, T Ordog, AV Matveyenko Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of beta cell function Science Advances, 2021;7(51):eabg6856. 2021 [PMID: 34910509] (ICC, Mouse) ICC Mouse
J Rui, S Deng, AL Perdigoto, G Ponath, R Kursawe, N Lawlor, T Sumida, M Levine-Rit, ML Stitzel, D Pitt, J Lu, KC Herold Tet2 Controls the Responses of beta cells to Inflammation in Autoimmune Diabetes Nature Communications, 2021;12(1):5074. 2021 [PMID: 34417463] (Flow Cytometry, Mouse) Flow Cytometry Mouse
M Kojima, H Takahashi, T Kuwashiro, K Tanaka, H Mori, I Ozaki, Y Kitajima, Y Matsuda, K Ashida, Y Eguchi, K Anzai Glucagon-Like Peptide-1 Receptor Agonist Prevented the Progression of Hepatocellular Carcinoma in a Mouse Model of Nonalcoholic Steatohepatitis Int J Mol Sci, 2020;21(16):. 2020 [PMID: 32785012] (IHC, Mouse) IHC Mouse
DM Elizondo, NZD Brandy, RLL da Silva, TR de Moura, J Ali, D Yang, MW Lipscomb Pancreatic islets seeded in a novel bioscaffold forms an organoid to rescue insulin production and reverse hyperglycemia in models of type 1 diabetes Sci Rep, 2020;10(1):4362. 2020 [PMID: 32152396] (IHC, Mouse) IHC Mouse
DG Alleva, M Rezaee, L Yip, G Ren, J Rosenberg, W Concepcion, A Escher, S Shabahang, AS Thakor Reversal of Hyperglycemia and Suppression of Type 1 Diabetes in the NOD Mouse with Apoptotic DNA Immunotherapy� (ADi�), ADi-100 Biomedicines, 2020;8(3):. 2020 [PMID: 32143316] (IHC-P, Mouse) IHC-P Mouse
FC Ning, N Jensen, J Mi, W Lindström, M Balan, L Muhl, U Eriksson, I Nilsson, D Nyqvist VEGF-B ablation in pancreatic beta-cells upregulates insulin expression without affecting glucose homeostasis or islet lipid uptake Sci Rep, 2020;10(1):923. 2020 [PMID: 31969592] (IHC, Mouse) IHC Mouse
Z Fang, C Weng, H Li, R Tao, W Mai, X Liu, L Lu, S Lai, Q Duan, C Alvarez, P Arvan, A Wynshaw-Bo, Y Li, Y Pei, F Jin, Y Li Single-Cell Heterogeneity Analysis and CRISPR Screen Identify Key ?-Cell-Specific Disease Genes Cell Rep, 2019;26(11):3132-3144.e7. 2019 [PMID: 30865899] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Cucak H, Mayer C, Tonnesen M, Thomsen L, Grunnet L, Rosendahl A Macrophage contact dependent and independent TLR4 mechanisms induce beta-cell dysfunction and apoptosis in a mouse model of type 2 diabetes. PLoS ONE, 2014;9(3):e90685. 2014 [PMID: 24594974] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Cucak H, Grunnet L, Rosendahl A Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization. J Leukoc Biol, 2014;95(1):149-60. 2014 [PMID: 24009176] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Show All 14 Publications.

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FAQs for Insulin Antibody (MAB1417). (Showing 1 - 1 of 1 FAQs).

  1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
    • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

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Blogs on Insulin. Showing 1-10 of 13 blog posts - Show all blog posts.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

COVID-19 and metabolic dysregulation: SARS-CoV-2 injures human exocrine and endocrine pancreas
Jamshed Arslan, Pharm D, PhD Humans rely on the pancreas for digesting food and generating energy from it. SARS-CoV-2-mediated damage to the exocrine pancreas is evident from the pancreatitis, pancreatic enlargeme...  Read full blog post.

Insulin signaling in adipocytes: Carbohydrate-signaling transcription factor ChREBP is the link between lipolytic enzyme Hormone-Sensitive Lipase and lipogenic enzyme ELOVL6
By Jamshed Arslan, Pharm. D., PhD. Insulin resistance in adipocytes is a major feature of metabolic syndrome   . Disrupted adipose tissue metabolism can lead to accumulation of lipid intermediates in insul...  Read full blog post.

Inhibiting incretin GIP hormone activity in mouse and monkey models to combat obesity
By Jamshed Arslan, Pharm. D., PhD. We live in a world where 39% of adults are overweight. Our meals trigger the secretion of various gut-derived metabolic hormones called incretins. Fats and carbohydrates in our die...  Read full blog post.

Insulin signaling in brain’s subfornical organ is crucial for regulating cardiometabolic profile
By Jamshed Arslan, Pharm. D., PhD. The hypothalamus is an insulin receptor-rich brain region. Insulin receptor signaling in the CNS can regulate blood pressure, for example, by increasing sympathetic outflow to th...  Read full blog post.

Eat responsibly: Epigenetic downregulation of Ankrd26 gene by long-term high-fat intake promotes obesity and inflammation
By Jamshed Arslan Pharm.D. White adipose tissue (WAT) represents the primary site to store energy in humans. WAT’s endocrine regulation of energy balance is controlled by nutritional status, exercise, and hormones l...  Read full blog post.

Application Focus: New Methods for iPSC Differentiation, Inducing a Mammary Fate
Discovery of the Key to PluripotencyInduced pluripotent stem cells (iPSCs) may be generated from a wide range of fully differentiated cells, and under optimal conditions may be prompted to differentiate into virtu...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

AMPK Alpha 1 and lipid metabolism of adipocytes
AMP-activated protein kinase (AMPK) is best known as a sensor of oxidative stress.  AMPK is activated by increased intracellular AMP levels, which are a result of alterations in cellular metabolism from causes such as hypoxia, changes in ATP, sene...  Read full blog post.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in ...  Read full blog post.

Showing 1-10 of 13 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol INS