Insulin Antibody (182410) [Unconjugated]

Insulin was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Insulin was detected in immersion fixed paraffin-embedded sections of human pancreas using Rat Anti-Human/Mouse/Bovine Insulin Monoclonal Antibody (Catalog # MAB1417) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in islet cells. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu, Bv
• Applications: IHC, CyTOF-ready, Flow, ICC/IF
• Clone: 182410
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Insulin Antibody (182410) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Insulin
• Specificity: Detects bovine, human, and mouse insulin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: INS
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.5-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Insulin Antibody (182410) [Unconjugated]

• IDDM2
• ILPR
• INS
• Insulin
• IRDN
• MODY10
• proinsulin

Background

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Insulin Antibody (MAB1417)(16)

Publications using MAB1417

• Y Wakabayash, T Miyatsuka, M Miura, M Himuro, T Taguchi, H Iida, Y Nishida, Y Fujitani, H Watada STAT3 suppression and beta-cell ablation enhance alpha-to-beta reprogramming mediated by Pdx1 Scientific Reports, 2022;12(1):21419. 2022 [PMID: 36496541] (IHC, Mouse)
• L Lei, Y Huan, Q Liu, C Li, H Cao, W Ji, X Gao, Y Fu, P Li, R Zhang, Z Abliz, Y Liu, S Liu, Z Shen Morus alba L. (Sangzhi) Alkaloids Promote Insulin Secretion, Restore Diabetic beta-Cell Function by Preventing Dedifferentiation and Apoptosis Frontiers in Pharmacology, 2022;13(0):841981. 2022 [PMID: 35308210] (IHC, Mouse)
• MR Brown, SK Sen, A Mazzone, TK Her, Y Xiong, JH Lee, N Javeed, CS Colwell, K Rakshit, NK LeBrasseur, A Gaspar-Mai, T Ordog, AV Matveyenko Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of beta cell function Science Advances, 2021;7(51):eabg6856. 2021 [PMID: 34910509] (ICC, Mouse)
• J Rui, S Deng, AL Perdigoto, G Ponath, R Kursawe, N Lawlor, T Sumida, M Levine-Rit, ML Stitzel, D Pitt, J Lu, KC Herold Tet2 Controls the Responses of beta cells to Inflammation in Autoimmune Diabetes Nature Communications, 2021;12(1):5074. 2021 [PMID: 34417463] (Flow Cytometry, Mouse)
• M Kojima, H Takahashi, T Kuwashiro, K Tanaka, H Mori, I Ozaki, Y Kitajima, Y Matsuda, K Ashida, Y Eguchi, K Anzai Glucagon-Like Peptide-1 Receptor Agonist Prevented the Progression of Hepatocellular Carcinoma in a Mouse Model of Nonalcoholic Steatohepatitis Int J Mol Sci, 2020;21(16):. 2020 [PMID: 32785012] (IHC, Mouse)
• M Kojima, H Takahashi, T Kuwashiro, K Tanaka, H Mori, I Ozaki, Y Kitajima, Y Matsuda, K Ashida, Y Eguchi, K Anzai Glucagon-Like Peptide-1 Receptor Agonist Prevented the Progression of Hepatocellular Carcinoma in a Mouse Model of Nonalcoholic Steatohepatitis Int J Mol Sci, 2020;21(16):. 2020 [PMID: 32785012] (IHC, Mouse)
• DM Elizondo, NZD Brandy, RLL da Silva, TR de Moura, J Ali, D Yang, MW Lipscomb Pancreatic islets seeded in a novel bioscaffold forms an organoid to rescue insulin production and reverse hyperglycemia in models of type 1 diabetes Sci Rep, 2020;10(1):4362. 2020 [PMID: 32152396] (IHC, Mouse)
• DG Alleva, M Rezaee, L Yip, G Ren, J Rosenberg, W Concepcion, A Escher, S Shabahang, AS Thakor Reversal of Hyperglycemia and Suppression of Type 1 Diabetes in the NOD Mouse with Apoptotic DNA Immunotherapy� (ADi�), ADi-100 Biomedicines, 2020;8(3):. 2020 [PMID: 32143316] (IHC-P, Mouse)
• FC Ning, N Jensen, J Mi, W Lindström, M Balan, L Muhl, U Eriksson, I Nilsson, D Nyqvist VEGF-B ablation in pancreatic beta-cells upregulates insulin expression without affecting glucose homeostasis or islet lipid uptake Sci Rep, 2020;10(1):923. 2020 [PMID: 31969592] (IHC, Mouse)
• Z Fang, C Weng, H Li, R Tao, W Mai, X Liu, L Lu, S Lai, Q Duan, C Alvarez, P Arvan, A Wynshaw-Bo, Y Li, Y Pei, F Jin, Y Li Single-Cell Heterogeneity Analysis and CRISPR Screen Identify Key ?-Cell-Specific Disease Genes Cell Rep, 2019;26(11):3132-3144.e7. 2019 [PMID: 30865899] (Flow Cytometry, Mouse)
• Cucak H, Mayer C, Tonnesen M, Thomsen L, Grunnet L, Rosendahl A Macrophage contact dependent and independent TLR4 mechanisms induce beta-cell dysfunction and apoptosis in a mouse model of type 2 diabetes. PLoS ONE, 2014;9(3):e90685. 2014 [PMID: 24594974] (Flow Cytometry, Mouse)
• Cucak H, Grunnet L, Rosendahl A Accumulation of M1-like macrophages in type 2 diabetic islets is followed by a systemic shift in macrophage polarization. J Leukoc Biol, 2014;95(1):149-60. 2014 [PMID: 24009176] (Flow Cytometry, Mouse)
• Hagberg C, Mehlem A, Falkevall A, Muhl L, Fam B, Ortsater H, Scotney P, Nyqvist D, Samen E, Lu L, Stone-Elander S, Proietto J, Andrikopoulos S, Sjoholm A, Nash A, Eriksson U Targeting VEGF-B as a novel treatment for insulin resistance and type 2 diabetes. Nature, 2012;490(7420):426-30. 2012 [PMID: 23023133] (IHC-P, Mouse)
• Kalis M, Bolmeson C, Esguerra JL, Gupta S, Edlund A, Tormo-Badia N, Speidel D, Holmberg D, Mayans S, Khoo NK, Wendt A, Eliasson L, Cilio CM Beta-cell specific deletion of Dicer1 leads to defective insulin secretion and diabetes mellitus. PLoS ONE, 2011;6(12):e29166. 2011 [PMID: 22216196] (ICC, Mouse)
• Jin CX, Li WL, Xu F, Geng ZH, He ZY, Su J, Tao XR, Ding XY, Wang X, Hu YP Conversion of immortal liver progenitor cells into pancreatic endocrine progenitor cells by persistent expression of Pdx-1. J. Cell. Biochem., 2008;104(1):224-36. 2008 [PMID: 17979180] (ICC, Mouse)
• Dowling P, O'Driscoll L, O'Sullivan F, Dowd A, Henry M, Jeppesen PB, Meleady P, Clynes M Proteomic screening of glucose-responsive and glucose non-responsive MIN-6 beta cells reveals differential expression of proteins involved in protein folding, secretion and oxidative stress. Proteomics, 2006;6(24):6578-87. 2006 [PMID: 17163442] (ICC, Mouse)

FAQs for Insulin Antibody (MAB1417). (Showing 1 - 1 of 1 FAQs).

1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
   • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

Bioinformatics

• Gene Symbol: INS