| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-53818SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24389SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11775SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC5294R-100UG | |||
| Carrier Free | IC5294T-100UG | |||
| Carrier Free | MAB5294-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54702-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44486 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76992-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08504 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08434 | |||
![Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P 0.9999), as compared to CQ (50 uM) only, a dose that did not completely inhibit autophagy. Data represent mean +- SEM. (H) N27 cells were treated with 62.5 or 125 uM of MnCl2 for 24h, then incubated with LysoSensor Yellow/Blue. The ratio of acidic (yellow) vs. neutral (blue) pH of lysosomes were quantified using a plate reader. (I) N27 cells were treated with Mn 125 uM, CQ50 uM or both for 24h, followed by immunoblotting for Atg5, a marker for autophagosomes, using Jess (ProteinSimple, Inc.) (J) Total protein per capillary was used as loading control to normalize the levels of Atg5. Mn did not further increase the accumulation of autophagosomes when combined with CQ versus CQ alone (P = 0.9998). All data represent mean +- SEM (n = 3-4 independent experiments with approximately 30 cells per experiment for E and G) and groups were compared by using Kruskal-Wallis ANOVA test with Dunn's post-hoc test; ns: non-significant. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-024-00708-w ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-53818_rabbit-polyclonal-atg5-antibody-30820241044550.jpg "Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P 0.9999), as compared to CQ (50 uM) only, a dose that did not completely inhibit autophagy. Data represent mean +- SEM. (H) N27 cells were treated with 62.5 or 125 uM of MnCl2 for 24h, then incubated with LysoSensor Yellow/Blue. The ratio of acidic (yellow) vs. neutral (blue) pH of lysosomes were quantified using a plate reader. (I) N27 cells were treated with Mn 125 uM, CQ50 uM or both for 24h, followed by immunoblotting for Atg5, a marker for autophagosomes, using Jess (ProteinSimple, Inc.) (J) Total protein per capillary was used as loading control to normalize the levels of Atg5. Mn did not further increase the accumulation of autophagosomes when combined with CQ versus CQ alone (P = 0.9998). All data represent mean +- SEM (n = 3-4 independent experiments with approximately 30 cells per experiment for E and G) and groups were compared by using Kruskal-Wallis ANOVA test with Dunn's post-hoc test; ns: non-significant. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24957904), licensed under a CC-BY license. Not internally tested by R&D Systems. Image collected and cropped by CiteAb from the following publication (//molecularneurodegeneration.biomedcentral.com/articles/10.1186/s13024-024-00708-w ), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Western Blot: ATG5 Antibody - BSA Free [NB110-53818] - Differences in neuronal autophagy & dendrite varicosity following HIV-1 Tat protein & morphine treatment. (A) Representative images of neurons transfected with a fluorescent reporter plasmid to monitor autophagic flux at 8 h following the indicated treatments. GFP (green) & GFP + mRFP (yellow) fluorescence are observed prior to the fusion of autophagosomes with lysosomes whereas only mRFP (red) fluorescence is present in post-fusion autolysosomes. DIC, differential interference contrast microscopy image. DAPI (blue) staining indicates cell nuclei. (B) Quantification of autolysosomes (red puncta) from (A). F(3,13) = 8.756, p = 0.0019; ∗p < 0.05 when compared to all other groups. (C) Western blotting analysis of the indicated autophagy associated protein levels at 24 h following the indicated treatments. GAPDH was used as a loading control. Blots are representative of three independent experiments. (D) Quantification of dendrite beading from (A). F(3,77) = 6.429, p = 0.0006; ∗p < 0.05 when compared to control cells. Error bars show the SEM. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fmicb.2015.00653/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-53818_rabbit-polyclonal-atg5-antibody-3102024162495.jpg)
![Western Blot: ATG5 Antibody [NBP2-24389] - Western blot analysis of human APGL5 in T98G cell lysate in 1) absence, 2) presence of immunizing peptide using this antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP2-24389-img0001.jpg "Western Blot: ATG5 Antibody [NBP2-24389] - Western blot analysis of human APGL5 in T98G cell lysate in 1) absence, 2) presence of immunizing peptide using this antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-24389] - Immunohistochemistry analysis of human kidney using this antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-24389-img0002.jpg)
![Western Blot: ATG5 Antibody [NBP3-11775] - Total protein from A431, U2Os, NIH 3T3, N2A, L929, RAW, PC12, Human Colon, Rat Colon and Mouse Colon were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 (NB110-53818) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP3-11775-img0003.jpg "Western Blot: ATG5 Antibody [NBP3-11775] - Total protein from A431, U2Os, NIH 3T3, N2A, L929, RAW, PC12, Human Colon, Rat Colon and Mouse Colon were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 (NB110-53818) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0008.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP3-11775] - Analysis of a FFPE tissue section of mouse testis using 1:200 dilution of ATG5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP3-11775-img0002.jpg)
![Flow Cytometry: ATG5 Antibody [NBP3-11775] - An intracellular stain was performed on Neuro2a cells with ATG5 Antibody NBP3-11775 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/ATG5-Antibody-Flow-Cytometry-NBP3-11775-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0007.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP3-11775] - Analysis of a FFPE tissue section of human testis using 1:200 dilution of ATG5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP3-11775-img0001.jpg)
![Flow Cytometry: ATG5 Antibody [NBP3-11775] - An intracellular stain was performed on THP-1 cells with ATG5 Antibody NBP3-11775 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/ATG5-Antibody-Flow-Cytometry-NBP3-11775-img0004.jpg)
![Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Western-Blot-NBP3-15854-img0003.jpg "Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts from normal (control) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Knockout-Validated-NBP3-15854-img0001.jpg)
![Immunoprecipitation: ATG5 Antibody (8L7X6) [NBP3-15854] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (NBP3-15854). Western blot was performed from the immunoprecipitate using ATG5 (NBP3-15854) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Immunoprecipitation-NBP3-15854-img0004.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726014.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517251919.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251720294.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726015.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517273524.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon cancer tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517213515.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-522025171896.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517284996.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded rat colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517152727.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517172826.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemisty analysis of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0010.jpg "Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemisty analysis of human endometrium shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemistry analysis of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0007.jpg)
![Western Blot: ATG5 Antibody [NBP2-54702] - Western blot analysis in control (vector only transfected HEK293T lysate) and ATG5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP2-54702-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP2-54702] - Immunocytochemistry analysis of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54702-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemistry analysis of human tonsil shows moderate to strong cytoplasmic positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0009.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemistry analysis of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0006.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemisty analysis of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells and lymphoid cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0008.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis in human prostate and skeletal muscle tissues using NBP2-88936 antibody. Corresponding ATG5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ATG5-Antibody-CL9508-Immunohistochemistry-Paraffin-NBP2-88936-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis in human prostate and skeletal muscle tissues using NBP2-88936 antibody. Corresponding ATG5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: ATG5 Antibody (CL9508) [NBP2-88936] - Western blot analysis in human cell line RH-30.](http://images.novusbio.com/fullsize/ATG5-Antibody-CL9508-Western-Blot-NBP2-88936-img0007.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-CL9508-Immunohistochemistry-Paraffin-NBP2-88936-img0001.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human prostate cancer shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-CL9508-Immunohistochemistry-Paraffin-NBP2-88936-img0002.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-CL9508-Immunohistochemistry-Paraffin-NBP2-88936-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ATG5-Antibody-CL9508-Immunohistochemistry-Paraffin-NBP2-88936-img0004.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (CL9508) [NBP2-88936] - Immunohistochemistry analysis of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ATG5-Antibody-CL9508-Immunohistochemistry-Paraffin-NBP2-88936-img0005.jpg)
![Western Blot: ATG5 Antibody [NBP1-76992] - IAV via Protein Expression Profile in Cell Lines. Antibodies: ATG5, beta-actin, and GAPDH. Secondary antibody: Goat anti-chicken or goat anti-mouse IgG HRP conjugate.](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP1-76992-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ATG5 Antibody [NBP1-76992] - IAV via Protein Expression Profile in Cell Lines. Antibodies: ATG5, beta-actin, and GAPDH. Secondary antibody: Goat anti-chicken or goat anti-mouse IgG HRP conjugate.")
![<b>Independent Antibodies Validation. </b>Western Blot: ATG5 Antibody [NBP1-76992] - Western blot analysis of mouse spleen tissue lysate with ATG5 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP1-76992-img0002.jpg)
![Western Blot: ATG5 Antibody [NBP1-76992] - Western blot analysis of various cell lysates, 15 ug of lysates per lane. Secondary antibody: Goat anti-chicken IgG HRP conjugate.](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP1-76992-img0003.jpg)
![Immunohistochemistry: ATG5 Antibody - BSA Free [NBP1-76992] - Immunohistochemistry Validation of ATG5 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ATG5 antibody at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-chicken IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-76992_chicken-polyclonal-atg5-antibody-2711202420405537.jpg)
![Western Blot: ATG5 Antibody (ATG5/2101) [NBP3-08504] - Western Blot Analysis of THP-1 cell and human Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2101).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2101-Western-Blot-NBP3-08504-img0003.jpg "Western Blot: ATG5 Antibody (ATG5/2101) [NBP3-08504] - Western Blot Analysis of THP-1 cell and human Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2101).")
![Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2101) [NBP3-08504] - Formalin-fixed, paraffin-embedded human Endometrium stained with ATG5 Mouse Monoclonal Antibody (ATG5/2101).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2101-Immunohistochemistry-Paraffin-NBP3-08504-img0002.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2101) [NBP3-08504] - Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2101).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2101-Immunohistochemistry-Paraffin-NBP3-08504-img0001.jpg)
![Protein Array: ATG5 Antibody (ATG5/2101) [NBP3-08504] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ATG5 Mouse Monoclonal Antibody (ATG5/2101).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2101-Protein-Array-NBP3-08504-img0004.jpg)
![Western Blot: ATG5 Antibody (ATG5/2101) [NBP3-07889] - Western Blot Analysis of THP-1 cell and human Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2101).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2101-Western-Blot-NBP3-07889-img0004.jpg "Western Blot: ATG5 Antibody (ATG5/2101) [NBP3-07889] - Western Blot Analysis of THP-1 cell and human Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2101).")
![Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2101) [NBP3-07889] - Formalin-fixed, paraffin-embedded human Endometrium stained with ATG5 Mouse Monoclonal Antibody (ATG5/2101).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2101-Immunohistochemistry-Paraffin-NBP3-07889-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2101) [NBP3-07889] - Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2101).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2101-Immunohistochemistry-Paraffin-NBP3-07889-img0002.jpg)
![Protein Array: ATG5 Antibody (ATG5/2101) [NBP3-07889] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ATG5 Mouse Monoclonal Antibody (ATG5/2101).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2101-Protein-Array-NBP3-07889-img0001.jpg)
![Western Blot: ATG5 Antibody (ATG5/2553) [NBP3-07880] - Western Blot Analysis of K562, U-87, A431 cell lysates using ATG5 Mouse Monoclonal Antibody (ATG5/2553).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2553-Western-Blot-NBP3-07880-img0005.jpg "Western Blot: ATG5 Antibody (ATG5/2553) [NBP3-07880] - Western Blot Analysis of K562, U-87, A431 cell lysates using ATG5 Mouse Monoclonal Antibody (ATG5/2553).")
![Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2553) [NBP3-07880] - Formalin-fixed, paraffin-embedded human Endometrium stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2553-Immunohistochemistry-Paraffin-NBP3-07880-img0003.jpg)
![Western Blot: ATG5 Antibody (ATG5/2553) [NBP3-07880] - Western Blot Analysis of THP-1 cell and human Brain tissue lysate using ATG5 Mouse Monoclonal Antibody (ATG5/2553).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2553-Western-Blot-NBP3-07880-img0004.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2553) [NBP3-07880] - Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2553-Immunohistochemistry-Paraffin-NBP3-07880-img0001.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody (ATG5/2553) [NBP3-07880] - Formalin-fixed, paraffin-embedded human Uterus stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).](http://images.novusbio.com/fullsize/ATG5-Antibody-ATG5-2553-Immunohistochemistry-Paraffin-NBP3-07880-img0002.jpg)