Western Blot: ATG12 Antibody [NBP2-15501] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG ...read more
Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Human lung cancer. ATG12 stained by ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: ATG12 Antibody [NBP2-15501] - Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE gel, diluted at 1:1000.
Genetic Strategies: Western Blot: ATG12 Antibody [NBP2-15501] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 ...read more
Western Blot: ATG12 Antibody [NBP2-15501] - Sample (50 ug of whole cell lysate) A: Rat brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary ...read more
Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Rat brain. ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunoprecipitation: ATG12 Antibody [NBP2-15501] - Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 ug of ATG12 antibody. Western blot analysis was performed using ATG12 antibody. ...read more
15 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP2-15501 in the following applications:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7), 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for ATG12 Antibody
Apg12 (autophagy 12, S. cerevisiae)-like
APG12 autophagy 12-like (S. cerevisiae)
ATG12 autophagy related 12 homolog (S. cerevisiae)
Autophagy-related protein 12
ubiquitin-like protein ATG12
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for ATG12 Antibody (NBP2-15501). (Showing 1 - 1 of 1 FAQs).
I used ATG12 antibody [NBP2-15501] to detect ATG12 and ATG12-ATG5 complex in rat myocardium tissue lysates by Western blot. In results, I found two bands, one was just below the 55kD, another was right at 15kD, very different from the images on our website. I do not see any article about its molecular weight, can you plaease confirm if the bands are of ATG12 or ATG12-ATG5? May I ask if you got any data on rat myocardium or any references?
Rat's ATG12 has a predicted MW of 15 kDa, so I think you have the right band for that. ATG12-ATG5 complex in rat samples will not run exactly as it does in human (band slightly above 55 kDa). At the moment, we are not aware of any publications that show the ATG12-ATG5 complex on a WB .You may consider using both an ATG5 and ATG12 antibody on their samples to see if the complex is detected with two different antibodies. Alternatively you can use KO lysates or siRNA to knockdown ATG5 or ATG12.
The LC3 A, B, C’s and 1, 2, 3’s By Christina Towers, PhD Autophagy is a catabolic process used to breakdown and recycle damaged proteins and organelles. It is a multistep process that, in its simplest form, consists of 4 steps: initiation, phago... Read full blog post.
Animal Models to Study Autophagy By Christina Towers, PhD What is autophagy?Autophagy is the catabolic process that degrades cytoplasmic material via the lysosome. The process of macroautophagy was originally characterized in yeast, where the... Read full blog post.
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