ATG12 Antibody

Images

 
Western Blot: ATG12 Antibody [NBP2-15501] - Sample (50 ug of whole cell lysate) A: Rat brain 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary ...read more
Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Human lung cancer. ATG12 stained by ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: ATG12 Antibody [NBP2-15501] - Sample (30 ug of whole cell lysate) A: NIH-3T3 12% SDS PAGE gel, diluted at 1:1000.
Genetic Strategies: Western Blot: ATG12 Antibody [NBP2-15501] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 ...read more
Western Blot: ATG12 Antibody [NBP2-15501] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: ATG12 Antibody [NBP2-15501] - Rat brain. ATG12 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunoprecipitation: ATG12 Antibody [NBP2-15501] - Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 ug of ATG12 antibody. Western blot analysis was performed using ATG12 antibody. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

ATG12 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Primate (96%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ATG12 Knockout HeLa Cell Lysate
Publications
Read Publications using
NBP2-15501 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Chimpanzee (96%), Pig (81%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for ATG12 Antibody

  • Apg12 (autophagy 12, S. cerevisiae)-like
  • APG12 autophagy 12-like (S. cerevisiae)
  • APG12
  • APG12HAPG12
  • APG12-like
  • ATG12 autophagy related 12 homolog (S. cerevisiae)
  • ATG12
  • Autophagy-related protein 12
  • FBR93
  • HAPG12
  • ubiquitin-like protein ATG12
  • yeast) homolog

Background

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ATG12 Antibody (NBP2-15501)(3)

Reviews for ATG12 Antibody (NBP2-15501) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for ATG12 Antibody (NBP2-15501). (Showing 1 - 1 of 1 FAQs).

  1. I used ATG12 antibody [NBP2-15501] to detect ATG12 and ATG12-ATG5 complex in rat myocardium tissue lysates by Western blot. In results, I found two bands, one was just below the 55kD, another was right at 15kD, very different from the images on our website. I do not see any article about its molecular weight, can you plaease confirm if the bands are of ATG12 or ATG12-ATG5? May I ask if you got any data on rat myocardium or any references? 
    • Rat's ATG12 has a predicted MW of 15 kDa, so I think you have the right band for that. ATG12-ATG5 complex in rat samples will not run exactly as it does in human (band slightly above 55 kDa). At the moment, we are not aware of any publications that show the ATG12-ATG5 complex on a WB.You may consider using both an ATG5 and ATG12 antibody on their samples to see if the complex is detected with two different antibodies. Alternatively you can use KO lysates or siRNA to knockdown ATG5 or ATG12. 

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on ATG12. Showing 1-10 of 15 blog posts - Show all blog posts.


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Autophagy independent roles of the core ATG proteins
By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation.  It is critical for removal of dam...  Read full blog post.

WIPI1 - An essential regulator of early autophagosome assembly
WD repeat domain phosphoinositide-interacting protein 1 (WIPI) is involved in the lysosomal degradation of cytoplasmic components during starvation-induced autophagy. WIPI1 is a seven bladed beta-propeller protein that provides a scaffold for the ...  Read full blog post.

ATG4C - A regulator of the early steps of autophagosome assembly
Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...  Read full blog post.

ATG16L2 - An autophagy-related protein with unknown functions
Autophagy is a process by which cells degrade and recycle damaged organelles or misfolded proteins. These various cargo are engulfed in a double-membrane structure called the autophagosome. The autophagosome then fuses with the lysosome to facilit...  Read full blog post.

ATG4D - A regulator of autophagy and apoptosis
Autophagy is an essential cellular process whereby damaged proteins and organelles are degraded and recycled. Autophagy, while happening constantly at a basal level, is tightly regulated and can be further induced under cellular stress. One of the...  Read full blog post.

ATG4B - a cysteine protease involved in autophagosome elongation
Autophagy can be broken down into 4 main stages: phagophore nucleation, autophagosome elongation, autophagosome docking and fusion with a lysosome, and vesicle breakdown and degradation. ATG4B is one of four ATG4 homologs (ATG4A, ATG4B, ATG4C, and...  Read full blog post.

ATG16L1 - a key player in the development of the autophagosome
Like apoptosis, autophagy is a highly regulated physiologic process that involves cellular degradation and recycling of organelles and macromolecules. Autophagy is a survival mechanism induced by states of stress, starvation, and infection. A do...  Read full blog post.

Showing 1-10 of 15 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol ATG12