TLR2 Antibody (TL2.1) [Biotin]

Western Blot: TLR2 Antibody (TL2.1) [Biotin] [NB100-56056] - Detection of TLR2 mRNA in multiple cell lines. No detection was observed in L363 or Fravel cell lines. [PMID: 23593278] Redegeld et al. 2013 PLoS One

Product Discontinued

TLR2 Antibody (TL2.1) [Biotin] Summary

• Immunogen: This antibody was raised by immunizing mice with CHO cells transfected with human TLR2 cDNA (Flo et al, 2000). The hybridoma supernatants were selected by flow cytometry.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLR2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1ul/1 million cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Immunoprecipitation 2-5 ug/ ml
  • Western Blot
• Application Notes: Immunocytochemistry/Immunofluorescence: please see Mempel et al. (2003) for details Immunohistochemistry (paraffin): please see Faure et al. (2001) for details Western Blot: please see Faure et al. (2001) for details. Use in ELISA reported in scientific literature (PMID: 23629653).
• Agonist:
  • Pam3CSK4, TLR1 and TLR2 Ligand NBP2-25297
  • SUCNR1/GPR91 Antibody NBP2-26239
  • MALP-2, TLR6 and TLR2 ligand NBP2-26219
• Antagonist:
  • TIRAP (TLR2 and TLR4) Inhibitor Peptide Set NBP2-29331
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371
• Control:

  TLR2 Overexpression Lysate

Reactivity Notes

Cross reacts with Dog and Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for TLR2 Antibody (TL2.1) [Biotin]

• CD282 antigen
• CD282
• TIL4CD282
• TLR2
• toll/interleukin 1 receptor-like 4
• Toll/interleukin-1 receptor-like protein 4
• toll-like receptor 2

Background

The Toll-like receptors (TLRs) in mammals comprise a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is differentially expressed in human cells. TLR2 is expressed in tonsils, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes expressed the highest level of TLR2 followed by CD15+ granulocytes, and CD19+ B-cells, CD3+ T-cells, and CD56+ NK cells did not express TLR2. The expression of TLR2 in different cell types is regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TLR2 Antibody (NB100-56056)(29)

Publications using NB100-56056

• Tsai CC, Lin CR, Tsai HY et al. The immunologically active oligosaccharides isolated from wheatgrass modulate monocytes via Toll-like receptor-2 signaling J Biol Chem 2013-05-01 [PMID: 23629653]
• Abdi J, Mutis T, Garssen J, Redegeld F. Characterization of the Toll-like receptor expression profile in human multiple myeloma cells. PLoS One. 2013-04-08 [PMID: 23593278]
  
  Details:
  Antibodies cited [multiple myeloma (MM) cell lines, human intestine tissue lysate, PBMC, mouse splenocytes] for WB (Figs 2, 3, 4A, 4B, 5, Table 1) and Flow (Intracellular): Figs 4C, 6-8, Table 1: 1. TLR1 (IMG-5012) 2. TLR2 (IMG-416A)3. TLR3 (IMG-315A)4 TL
• Harman AN, Bye CR, Nasr N et al. Identification of lineage relationships and novel markers of blood and skin human dendritic cells. J Immunol. 2013-01-01 [PMID: 23183897]
  
  Details:
  Antibodies cited in Fig 4B for flow cytometric analysis of TLR expression in human monocyte-derived dendritic cells (MDDC), CD14+ monocytes, myeloid DC, and plasmacytoid DC:1. TLR2-FITC, clone T2.1 (IMG-416C): Flow (cell surface)2. TLR4-PE, clone HTA124 (
• Shimizu T, Kida Y, Kuwano K. Cytoadherence-dependent induction of inflammatory responses by Mycoplasma pneumoniae. Immunology. 2011-05-01 [PMID: 21320122]
  
  Details:
  TLR2/CD282 (IMG-416A). WB: THP-1 cells treated with living or heat killed M. pneumoniae, Fig 3.
• Dasgupta G, Chentoufi AA, You S et al. Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses. Invest Ophthalmol Vis Sci. 2011-05-17 [PMID: 21273544]
  
  Details:
  Products cited: Flow (intracellular), Fig 1A ( CD4+CD25+ regulatory T cells purified from rabbit conjunctiva) & Fig 2A (CD11b+ cells isolated from rabbit conjunctiva): TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315D), TLR4/CD284 (IMG-417D), TLR8/CD288 (IMG
• Oberg HH, Ly TT, Ussat S et al. Differential but direct abolishment of human regulatory T cell suppressive capacity by various TLR2 ligands. J Immunol. 2010-05-01 [PMID: 20363971]
  
  Details:
  Flow(intracellular) cytometry: TLR2/CD282 PE (IMG-416D) and TLR6/CD286 (IMG-304A). T cells and Tregs were separated from freshly isolated human PBMCs, Fig S3.
• Ding J, Rapista A, Teleshova N et al. Neisseria gonorrhoeae enhances HIV-1 infection of primary resting CD4+ T cells through TLR2 activation. J Immunol. 2010-03-15 [PMID: 20147631]
  
  Details:
  TLR2/CD282 (IMG-416C). Flow (Cell surface), Fig 6D (human CD4+T cells).
• Rydberg C, Mansson A, Uddman R et al. Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas. Immunology. 2009-09-01 [PMID: 19740321]
  
  Details:
  TLR2/CD282 PE (IMG-416D), TLR3/CD283 (IMG-315A), TLR3/CD283 (IMG-315D), TLR5 PE (IMG-663D), TLR5 (IMG-663). 1. IHC(paraffin): Human head and neck squamous (HNSCC) cell carcinomas showing keratinized cell carcinoma from the larynx stained with TLR2 (IMG-416A) and TLR5 antibodies (IMG-663A), Fig 1A, 1B, & 1C. 2. Flow (intracellular): TLR2 PE (IMG-416D), TLR3 PE (IMG-315D), and TLR5 PE (IMG-663D) antibodies were used in human bronchial epithelial (NL-20) and human pharyngeal squamous (Detroit-562) carcinoma cell line, Fig 2C.
• Yang X, Fullerton DA, Su X et al Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2. J Am Coll Cardiol. 2009-02-10 [PMID: 19195606]
  
  Details:
  Citation using the Azide Free version of this antibody.
• Ji S, Shin JE, Kim YS et al. Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. Infect Immun 2009-03-01 [PMID: 19103770]
• Guzy C, Paclik D, Schirbel A et al. The probiotic Escherichia coli strain Nissle 1917 induces gammadelta T cell apoptosis via caspase- and FasL-dependent pathways. Int Immunol. 2008-07-01 [PMID: 18448456]
  
  Details:
  Antibodies cited [Flow (Cell Surface) (human PBMCs), Fig. 5]: 1. TLR2 FITC [IMG-416C] 2. TLR4 FITC [IMG-417C].
• Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008-07-15 [PMID: 18439687]
  
  Details:
  Canine (dog) PBMC subpopulations: 1. 10083K [IC-Flow (Intracellular Staining Flow Assay) Kit.
• Prabha C, Rajashree P, Sulochana DD. TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid. Immunol Lett. 2008-04-15 [PMID: 18295348]
  
  Details:
  TLR2- FITC (IMG-416C): Flow (cell surface): Figs. 1A, B (human CD4+T cells, CD8+T cells, B cells, CD16+56+ cells and monocytes); 2(CD4+T cells); 4A, B (human Treg cells). Flow (intracellular): Fig. 3A, B (CD4+T cells) 2. TLR4- FITC (IMG-417C).Flow (cell surface): Figs. 1B, C (human CD4+T cells, CD8+T cells, B cells, CD16+56+ cells and monocytes); 2(CD4+T cells); 4A, B (human Treg cells). Flow (intracellular): Fig. 3A, B (CD4+T cells).
• Pegu A, Qin S, Fallert Junecko BA et al. Human lymphatic endothelial cells express multiple functional TLRs. J Immunol. 2008-03-01 [PMID: 18292566]
  
  Details:
  Antibodies cited [Flow (Cell surface) and Flow (Intracellular), human lymphatic endothelial cells, Fig. 1D]: 1. IMG-663C (TLR5-FITC) 2. IMG-304C (TLR6-FITC).
• Wong CK, Cheung PF, Ip WK et al. Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils. Am J Respir Cell Mol Biol. 2007-07-01 [PMID: 17332440]
  
  Details:
  Human blood eosinophils and neutrophils from buffy coat: For WB, Fig. 1A: TLR1 (IMG-5012), TLR5 (IMG-664), TLR6 (IMG-304A), TLR7 (IMG-540), TLR8 (IMG-321A), TLR9 (IMG-305A). For Flow (Intracellular) and Flow (Surface), Fig. 1B: TLR1 (IMG-5021), TLR2 (IMG-416C), TLR3 (IMG-315C), TLR4 (IMG-417C), TLR5 (IMG-663C), TLR6 (IMG-304C), TLR7 (IMG-665A), TLR8 (IMG-321C), TLR9 (IMG-305C).
• Menzies BE, Kenoyer A. Signal transduction and nuclear responses in Staphylococcus aureus-induced expression of human beta-defensin 3 in skin keratinocytes. Infect Immun. 2006-12-01 [PMID: 16954397]
  
  Details:
  TLR2 [IMG-416E] 1. FA (human keratinocytes), Fig. 2.
• Xu S, Koldovsky U, Xu M et al High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production. Surgery. 2006-08-01 [PMID: 16904966]
  
  Details:
  Citation using the Azide Free version of this antibody.
• Shimizu T, Kida Y, Kuwano K. A dipalmitoylated lipoprotein from Mycoplasma pneumoniae activates NF-kappa B through TLR1, TLR2, and TLR6. J Immunol. 2005-10-01 [PMID: 16177110]
  
  Details:
  TLR2 [IMG-416E] 1. FA (THP-1 cells), Fig. 2.
• Srivastava MD, Srivastava BI. Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line. Leuk Res. 2005-07-01 [PMID: 15927677]
  
  Details:
  IF/ICC in SRIK-NKL cells: TLR2 (IMG416), TLR4 (IMG-417), TLR7 (IMG-581), and TLR9 (IMG-305).
• Cognasse F, Hamzeh H, Chavarin P et al. Evidence of Toll-like receptor molecules on human platelets. Immunol Cell Biol. 2005-04-01 [PMID: 15748217]
  
  Details:
  TLR2-PE (IMG-416D), TLR4-PE (IMG-417D), TLR6 (IMG-304A), TLR8-PE (IMG-321D), TLR9-PE (IMG-305D). Applications: Intracellular Flow Cytomety and Cell Surface Flow Cytometry: Figs 1 and 2. A comparison of staining results, intracellular versus cell surface flow cytometry is shown. Cell type: Human platelets.
• Bazzocchi C, Mortarino M, Comazzi S et al. Expression and function of Toll-like receptor 2 in canine blood phagocytes. Vet Immunol Immunopathol. 2005-03-10 [PMID: 15661327]
  
  Details:
  TLR2 [IMG-416A] 1. Flow (whole blood cells from dogs), Fig. 3.
• Zhong F, Cao W, Chan E et al. Deviation from major codons in the Toll-like receptor genes is associated with low Toll-like receptor expression Immunology 2005-01-01 [PMID: 15606798]
  
  Details:
  This citation used the FITC version of this antibody.
• Gibson FC 3rd, Hong C, Chou HH et al. Innate immune recognition of invasive bacteria accelerates atherosclerosis in apolipoprotein E-deficient mice. Circulation. 2004-06-08 [PMID: 15123526]
  
  Details:
  TLR2 [IMG-416]: 1. IHC:P (aortic tissue), Fig. 2b 2. Flow (Cell Surface) (P gingivalis, mutant P gingivalis), Figs. 3a, 3b.
• Mempel M, Voelcker V, Kollisch G et al. Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent. J Invest Dermatol. 2003-12-01 [PMID: 14675188] (ICC/IF, Human)
  
  Details:
  TLR2 (IMG-416) 2. TLR4 (IMG-417) [IF/ICC, Fig.2A and 2D (human keratinocytes)].
• Lotz Sonja, Aga Eresso, Wilde Inga et al. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 2004-03-01 [PMID: 14673018] (FLOW)
• Medvedev AE, Lentschat A, Wahl LM et al. Dysregulation of LPS-induced Toll-like receptor 4-MyD88 complex formation and IL-1 receptor-associated kinase 1 activation in endotoxin-tolerant cells. J Immunol. 2002-11-01 [PMID: 12391239]
  
  Details:
  TLR2 (IMG-416A): 1. WB (human monocytes), Fig. 3 2. Flow (Cell Surface) (human monocytes), Fig. 4.
• Flo TH, Halaas O, Torp S et al. Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol. 2001-03-01 [PMID: 11261796]
  
  Details:
  Antibodies cited: TLR2 (IMG-416): 1. Flow (Cell Surface): Figs 1 (whole blood), 5 (granulocytes), and 6 (monocytes) 2. IP: Fig 2 (monocytes) 3. IF/ICC: Fig 3 (monocyte-derived macrophages).
• Faure E, Thomas L, Xu H et al. Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappa B activation. J Immunol 2001-02-01 [PMID: 11160251]
  
  Details:
  This citation used the Biotin version of this antibody.
• Flo TH, Halaas O, Lien E et al. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol. 2000-02-15 [PMID: 10657659]
  
  Details:
  TLR2 (IMG-416): 1. Generation of the IMG-416 TL2.1 antibody clone, recognizing a TLR2-associated epitope (Materials and Methods).

Bioinformatics

• Gene Symbol: TLR2