Streptavidin Native Protein Summary
Description |
Native Streptavidin. (Accession #: P22629) |
Preparation Method |
Streptavidin Alkaline Phosphatase was prepared from electrophoretically pure Streptavidin. Streptavidin Alkaline Phosphatase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin. |
Protein/Peptide Type |
Native Protein |
Purity |
Immunoelectrophoresis (IEP) |
Applications/Dilutions
Dilutions |
- Western Blot 1:1000 - 1:4000
- ELISA 1:8000-1:32000
- Immunohistochemistry 1:200 - 1:1000
|
Application Notes |
Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate. |
Packaging, Storage & Formulations
Storage |
Store at 4C. Do not freeze. |
Buffer |
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0. 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative |
0.01% Sodium Azide |
Concentration |
1.0 mg/ml |
Purity |
Immunoelectrophoresis (IEP) |
Alternate Names for Streptavidin Native Protein
Background
Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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