Streptavidin Native Protein Summary
Native Bacteria Streptavidin
Source: Electrophoretically pure Streptavidin (Accession #:P22629)
This product was prepared from electrophoretically pure Streptavidin. Alkaline Phosphatase conjugated Streptavidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin.
| Protein/Peptide Type
- ELISA 1:8000 - 1:32000
- Immunohistochemistry 1:200 - 1:1000
- Western Blot 1:1000 - 1:4000
This product has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.05% Sodium Azide
Store vial at 4C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Alternate Names for Streptavidin Native Protein
- SA V1; SA V2; Streptavidin V1; Streptavidin V2
Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed
for 1 year from date of receipt.
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