Streptavidin Native Protein

Western Blot: Streptavidin Native Protein [NBP2-29371] - Lane 1: Biotin conjugated Guinea Pig IgG Load: 50 ng per lane Secondary antibody: STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa for Guinea Pig IgG.

• Reactivity: Ba
• Applications: WB, ELISA, IHC
• Conjugate: Alkaline Phosphatase

Streptavidin Native Protein Summary

• Description: Native Bacteria Streptavidin
  
  Source: Electrophoretically pure Streptavidin (Accession #:P22629)
  
  Store vial at 4C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Preparation Method: This product was prepared from electrophoretically pure Streptavidin. Alkaline Phosphatase conjugated Streptavidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin.
• Protein/Peptide Type: Native Protein
• Purity: Immunoelectrophoresis (IEP)

Applications/Dilutions

• Dilutions:
  • ELISA 1:8000 - 1:32000
  • Immunohistochemistry 1:200 - 1:1000
  • Western Blot 1:1000 - 1:4000
• Application Notes: This product has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.05% Sodium Azide
• Purity: Immunoelectrophoresis (IEP)

Alternate Names for Streptavidin Native Protein

• SA V1; SA V2; Streptavidin V1; Streptavidin V2
• Streptavidin

Background

Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Uniprot:
  • P22629(Bacteria)