Recombinant Mouse IFN-gamma Protein


Recombinant Mouse IFN-gamma (Catalog # 485‑ML) demonstrates anti-viral activity in L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.3-0.9 ng/mL.
1 μg/lane of Recombinant Mouse IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 11-12 kDa.

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Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse IFN-gamma Protein Summary

Details of Functionality
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 0.3-0.9 ng/mL.
E. coli-derived mouse IFN-gamma protein
His23-Cys155, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
15.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
11-12 kDa, reducing conditions
Read Publications using
485-MI in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol,Tween® 80 and TCEP with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse IFN-gamma Protein

  • IFG
  • IFI
  • IFNG
  • IFNgamma
  • IFN-gamma
  • Immune interferon
  • interferon gamma
  • interferon, gamma


Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature mouse IFN-gamma exists as a noncovalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with rat IFN-gamma and 38%-44% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma . IFN-gamma dimers bind to IFN‑ gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up-regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Gray, P.W. and D.V. Goeddel (1983) Proc. Natl. Acad. Sci. 80:5842.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.

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Publications for IFN-gamma (485-MI)(412)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Transgenic Mouse.

We have publications tested in 8 applications: Bioassay, Cell Cultulre, Cell Culture, Differentiation, ELISA (Standard), ELISA Control, In Vivo, Stimulation.

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Showing Publications 1 - 10 of 412. Show All 412 Publications.
Publications using 485-MI Applications Species
Ono, K;Sato, J;Suzuki, H;Sawada, M; Distribution of Signal Peptides in Microvesicles from Activated Macrophage Cells International journal of molecular sciences 2023-07-28 [PMID: 37569508] (Bioassay, Mouse) Bioassay Mouse
Bod, L;Kye, YC;Shi, J;Torlai Triglia, E;Schnell, A;Fessler, J;Ostrowski, SM;Von-Franque, MY;Kuchroo, JR;Barilla, RM;Zaghouani, S;Christian, E;Delorey, TM;Mohib, K;Xiao, S;Slingerland, N;Giuliano, CJ;Ashenberg, O;Li, Z;Rothstein, DM;Fisher, DE;Rozenblatt-Rosen, O;Sharpe, AH;Quintana, FJ;Apetoh, L;Regev, A;Kuchroo, VK; B-cell-specific checkpoint molecules that regulate anti-tumour immunity Nature 2023-06-21 [PMID: 37344597] (Cell Culture, Mouse) Cell Culture Mouse
Zhai, R;Lenga Ma Bonda, W;Leclaire, C;Saint-Béat, C;Theilliere, C;Belville, C;Coupet, R;Blondonnet, R;Bouvier, D;Blanchon, L;Sapin, V;Jabaudon, M; Effects of sevoflurane on lung epithelial permeability in experimental models of acute respiratory distress syndrome Journal of translational medicine 2023-06-18 [PMID: 37331963] (Bioassay, Mouse) Bioassay Mouse
Baars, I;Jaedtka, M;Dewitz, LA;Fu, Y;Franz, T;Mohr, J;Gintschel, P;Berlin, H;Degen, A;Freier, S;Rygol, SM;Schraven, B;Kahlfuss, S;van Zandbergen, G;Müller, AJ; Leishmania major drives host phagocyte death and cell-to-cell transfer depending on intracellular pathogen proliferation rate JCI insight 2023-06-13 [PMID: 37310793] (Bioassay, Mouse) Bioassay Mouse
Waugh, KA;Minter, R;Baxter, J;Chi, C;Galbraith, MD;Tuttle, KD;Eduthan, NP;Kinning, KT;Andrysik, Z;Araya, P;Dougherty, H;Dunn, LN;Ludwig, M;Schade, KA;Tracy, D;Smith, KP;Granrath, RE;Busquet, N;Khanal, S;Anderson, RD;Cox, LL;Estrada, BE;Rachubinski, AL;Lyford, HR;Britton, EC;Fantauzzo, KA;Orlicky, DJ;Matsuda, JL;Song, K;Cox, TC;Sullivan, KD;Espinosa, JM; Triplication of the interferon receptor locus contributes to hallmarks of Down syndrome in a mouse model Nature genetics 2023-06-05 [PMID: 37277650] (Bioassay, Transgenic Mouse) Bioassay Transgenic Mouse
Nohara, LL;Ellis, SLS;Dreier, C;Dada, S;Saranchova, I;Munro, L;Pfeifer, CG;Coyle, KM;Morrice, JR;Shim, DJS;Ahn, P;De Voogd, N;Williams, DE;Cheng, P;Garrovillas, E;Andersen, RJ;Jefferies, WA; A novel cell-based screen identifies chemical entities that reverse the immune-escape phenotype of metastatic tumours Frontiers in pharmacology 2023-05-15 [PMID: 37256225] (Bioassay, Mouse) Bioassay Mouse
Wayne, CR;Bremner, L;Faust, TE;Dur�n-Laforet, V;Ampatey, N;Ho, SJ;Feinberg, PA;Arvanitis, P;Ciric, B;Ruan, C;Elyaman, W;Delaney, SL;Vargas, WS;Swedo, S;Menon, V;Schafer, DP;Cutforth, T;Agalliu, D; Distinct Th17 effector cytokines differentially promote microglial and blood-brain barrier inflammatory responses during post-infectious encephalitis bioRxiv : the preprint server for biology 2023-05-09 [PMID: 37215000] (Bioassay, Mouse) Bioassay Mouse
Naito, Y;Koyama, S;Masuhiro, K;Hirai, T;Uenami, T;Inoue, T;Osa, A;Machiyama, H;Watanabe, G;Sax, N;Villa, J;Kinugasa-Katayama, Y;Nojima, S;Yaga, M;Hosono, Y;Okuzaki, D;Satoh, S;Tsuda, T;Nakanishi, Y;Suga, Y;Morita, T;Fukushima, K;Nishide, M;Shiroyama, T;Miyake, K;Iwahori, K;Hirata, H;Nagatomo, I;Yano, Y;Tamiya, M;Kumagai, T;Takemoto, N;Inohara, H;Yamasaki, S;Yamashita, K;Aoshi, T;Akbay, EA;Hosen, N;Shintani, Y;Takamatsu, H;Mori, M;Takeda, Y;Kumanogoh, A; Tumor-derived semaphorin 4A improves PD-1-blocking antibody efficacy by enhancing CD8+ T cell cytotoxicity and proliferation Science advances 2023-05-19 [PMID: 37205755] (Bioassay, Transgenic Mouse, Mouse) Bioassay Transgenic Mouse, Mouse
Slieker, RC;Donnelly, LA;Akalestou, E;Lopez-Noriega, L;Melhem, R;G�ne?, A;Abou Azar, F;Efanov, A;Georgiadou, E;Muniangi-Muhitu, H;Sheikh, M;Giordano, GN;�kerlund, M;Ahlqvist, E;Ali, A;Banasik, K;Brunak, S;Barovic, M;Bouland, GA;Burdet, F;Canouil, M;Dragan, I;Elders, PJM;Fernandez, C;Festa, A;Fitipaldi, H;Froguel, P;Gudmundsdottir, V;Gudnason, V;Gerl, MJ;van der Heijden, AA;Jennings, LL;Hansen, MK;Kim, M;Leclerc, I;Klose, C;Kuznetsov, D;Mansour Aly, D;Mehl, F;Marek, D;Melander, O;Niknejad, A;Ottosson, F;Pavo, I;Duffin, K;Syed, SK;Shaw, JL;Cabrera, O;Pullen, TJ;Simons, K;Solimena, M;Suvitaival, T;Wretlind, A;Rossing, P;Lyssenko, V;Legido Quigley, C;Groop, L;Thorens, B;Franks, PW;Lim, GE;Estall, J;Ibberson, M;Beulens, JWJ;'t Hart, LM;Pearson, ER;Rutter, GA; Identification of biomarkers for glycaemic deterioration in type 2 diabetes Nature communications 2023-05-03 [PMID: 37137910] (Bioassay, Mouse) Bioassay Mouse
BD Hobson, AT Stanley, MB De Los San, B Culbertson, EV Mosharov, PA Sims, D Sulzer Conserved and cell type-specific transcriptional responses to IFN-gamma in the ventral midbrain Brain, Behavior, and Immunity, 2023-04-24;0(0):. 2023-04-24 [PMID: 37100211] (Bioassay, Mouse) Bioassay Mouse
Show All 412 Publications.

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  1. Is Recombinant Human IFN-gamma Protein - Clinical grade or preclinical ?
    • We are research use only.

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Blogs on IFN-gamma.

Tired T cells: Hypoxia Drives T cell Exhaustion in the Tumor Microenvironment
By Hunter MartinezThe paradigm shifting view of the immune system being leveraged to target cancer has led to numerous therapeutic breakthroughs. One major cell group responsible for this revelation is a T cell. ...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.

  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

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Gene Symbol Ifng