Streptavidin Native Protein

Western Blot: Streptavidin Full length Protein [NBP2-29370] - Lane 1: Human IL-7. Lane 2: none. Load: 50 ng per lane. Primary antibody: Human IL-7 Biotin Conjugated antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: 5% BLOTTO 30 min at RT. Predicted/Observed size: 17 kDa, 17 kDa for Human IL-7. Other band(s): none.

• Reactivity: Ba
• Applications: WB, ELISA, ICC/IF, IHC
• Conjugate: HRP
• Concentration: LYOPH

Streptavidin Native Protein Summary

• Description: Native Bacteria Streptavidin
  
  Source: Chromatographically purified streptavidin (Accession #:P22629)
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Preparation Method: This product was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase conjugate was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.
• Protein/Peptide Type: Native Protein
• Purity: Chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000 - 1:200000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:10000 - 1:40000
• Application Notes: This product has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. This product can be utilized in Immunohistochemistry, Immunofluorescence, immuno-EM, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Chromatography
• Reconstitution Instructions: Reconstitute with 1.0 ml deionized water.

Alternate Names for Streptavidin Native Protein

• SA V1; SA V2; Streptavidin V1; Streptavidin V2
• Streptavidin

Background

Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P22629(Bacteria)