p62/SQSTM1 Antibody

Images

 
Western Blot: p62/SQSTM1 Antibody [NBP1-42821] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - Confocal immunofluorescent analysis of HeLa cells using p62/SQSTM1 antibody (NBP1-42821, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG ...read more
Immunocytochemistry/ Immunofluorescence: p62/SQSTM1 Antibody [NBP1-42821] - SQSTM1 staining in of Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
   

Biological Strategies

     

p62/SQSTM1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human p62/SQSTM1 protein (within residues 350-400). [Swiss-Prot Q13501]
Localization
Cytoplasm. Late endosome. Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SQSTM1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC9)
Control Peptide
p62/SQSTM1 Peptide (NBP1-42821PEP)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-42821 in the following applications:

Publications
Read Publication using
NBP1-42821 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p62/SQSTM1 Antibody

  • A170
  • EBI3-associated protein of 60 kDa
  • EBI3-associated protein p60
  • EBIAP
  • ORCA
  • OSIL
  • oxidative stress induced like
  • p60PDB3
  • p62
  • p62B
  • Paget disease of bone 3
  • PDB3
  • phosphotyrosine independent ligand for the Lck SH2 domain p62
  • Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
  • Sequestosome 1
  • sequestosome-1
  • SQSTM1
  • Ubiquitin-binding protein p62
  • ZIP3

Background

p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adapter protein conserved in vertebrates and it can be induced by proteasomal inhibitor PSI, PGJ2/prostaglandin J2 as well as phorbol 12-myristate 13-acetate (PMA). SQSTM1 has the ability of ubiquitin binding as well as regulating NFkB1 activation by TNF-alpha, NGF (nerve growth factor) and interleukin-1. SQSTM1 acts as an adapter that mediates the interaction between TRAF6 and CYLD, and SQSTM1-TRAF6 interaction leads to K63-linked polyubiquitination of TRAF6 followed by subsequent activation of NFkB pathway. SQSTM1 plays a role in TITIN/TTN downstream signaling in muscle cells and regulate signaling cascades through ubiquitination. SQSTM1 is essential both for formation and autophagic degradation of polyubiquitin-containing bodies which are known as aggresome-like induced structures (ALIS) and SQSTM1 connects ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members. Moreover, while autophagy modulates SQSTM1 protein levels, SQSTM1 suppresses autophagy via activation of mTORC1. SQSTM1 interacts with KEAP1, which is a cytoplasmic inhibitor of NRF2, a key transcription factor involved in cellular responses to oxidative stress. SQSTM1 functions as a signaling hub for various signal transduction pathways, apoptosis, cell differentiation, apoptosis, immune response, regulation of K+ channels and Nrf2 activation, and its dysregulation is associated with Paget disease of bone and tumorigenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p62/SQSTM1 Antibody (NBP1-42821)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for p62/SQSTM1 Antibody (NBP1-42821) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-42821:
Filter by Applications
ICC
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry p62/SQSTM1 NBP1-42821
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reviewed by:
Anony- mous
ICC Human 06/12/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsConfocal immunofluorescent analysis of HeLa cells using p62/SQSTM1 antibody (NBP1-42821, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Product General Protocols

View specific protocols for p62/SQSTM1 Antibody (NBP1-42821): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for p62/SQSTM1 Antibody (NBP1-42821) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-42821AF405
Alexa Fluor 488 Labeled NBP1-42821AF488
Alexa Fluor 647 Labeled NBP1-42821AF647
Alexa Fluor 700 Labeled NBP1-42821AF700
Biotin Labeled NBP1-42821B
DyLight 405 Labeled NBP1-42821V
DyLight 488 Labeled NBP1-42821G
DyLight 550 Labeled NBP1-42821R
DyLight 650 Labeled NBP1-42821C
FITC Labeled NBP1-42821F
HRP Labeled NBP1-42821H

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Bioinformatics Tool for p62/SQSTM1 Antibody (NBP1-42821)

Discover related pathways, diseases and genes to p62/SQSTM1 Antibody (NBP1-42821). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for p62/SQSTM1 Antibody (NBP1-42821)

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Blogs on p62/SQSTM1.

Key Targets in Apoptosis, Necroptosis, and Autophagy
Cell death/recycling pathways such as apoptosis, necroptosis, and autophagy are an integral part of the growth, development, homeostasis as well as the pathophysiology in the life of living organisms. These signaling pathways are highly regulated and...  Read full blog post.

Required proteins for p62/SQSTM1 regulation and a role for p62/SQSTM1 in neuronal autophagy
Autophagy is a crucial cellular process that clears the cell of protein aggregates, toxins, and damaged cell products. Accumulation of toxins, damaged cell products and unwanted proteins has been proven to play a role in aging and many forms of dis...  Read full blog post.

ATG4C - A regulator of the early steps of autophagosome assembly
Autophagy is an important cellular process that maintains homeostasis by degrading and recycling damaged proteins and organelles. Autophagy receptors, such as p62/SQSTM1, recognize these intracellular cargo and mediate their engulfment by the doubl...  Read full blog post.

p62/SQSTM1 - targeting ubiquitinated proteins for autophagic degradation
During autophagy ubiquitinated cargo or substrates are engulfed in a double-membrane autophagosome and transported to the lysosome for degradation. This process is important for maintaining cellular homeostasis and for degrading damaged organelles...  Read full blog post.

LC3/LC3B - measuring autophagosome formation and autophagic flux
Microtubule-associated protein-1 light chain 3 (LC3/LC3B) is a ubiquitin-like protein involved in the formation of the autophagosome. It is homologous to the yeast Atg8 protein. Autophagosomes are important for the degradation and recycling of intr...  Read full blog post.

p62/SQSTM1 (sequestosome 1)
p62/SQSTM1 (sequestosome 1) is ubiquitously-expressed cytoplasmic/adaptor protein. SQSTM1 functions as a signaling hub for various signal transduction pathways involved in apoptosis, cell differentiation, apoptosis, immune response, and K+ channel reg...  Read full blog post.

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Recent Reviews

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1
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Anony- mous
06/12/2014
Application: ICC
Species: Human

Bioinformatics

Gene Symbol SQSTM1
Entrez
Uniprot