HeLa Nuclear Cell Lysate

Images

 
Western Blot: HeLa Nuclear Cell Lysate [NB800-PC9] - Analysis of Histone H3 (NBP1-30141) uisng HeLa nuclear lysate [NB800-PC9].
SDS-Page: HeLa Nuclear Cell Lysate [NB800-PC9] - Staining of HeLa nuclear lysate using NB800-PC9.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PAGE
Concentration
1.0 mg/ml

Order Details

HeLa Nuclear Cell Lysate Summary

Purity
Multi-step

Applications/Dilutions

Application Notes
Ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5 minutes and rapidly cool. If dissociating conditions are desired add reducing agent prior to heating. The recommended loading volume per lane is 10-30 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Multi-step

Lysate Details for Array

Type
Cell
Subcellular Fraction
Nuclear

Notes

Store at -70C or COLDER. For extended storage, aliquot Nuclear Extract to minimize freeze/thaw cycles.

Background

This nuclear cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Other Available Formats

NB800-PC9

Additional HeLa Products

Array NB800-PC9

Blogs on HeLa.

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