HeLa Nuclear Cell Lysate


Western Blot: HeLa Nuclear Cell Lysate [NB800-PC9] - Analysis of Histone H3 (NBP1-30141) using HeLa nuclear lysate [NB800-PC9].
SDS-Page: HeLa Nuclear Cell Lysate [NB800-PC9] - Staining of HeLa nuclear lysate using NB800-PC9.
Coomassie stained SDS-PAGE of 25 ug of Human Derived HeLa Nuclear Cell Lysate (Ready-to-Use) separated in a 4-20% gradient gel under non-reducing conditions. Molecular weight standards are shown on the left.

Product Details

Reactivity HuSpecies Glossary
Applications WB, PAGE

Order Details

HeLa Nuclear Cell Lysate Summary

Store at -70C or COLDER. For extended storage, aliquot Nuclear Extract to minimize freeze/thaw cycles.
The cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 uM Aprotinin, 5 uM Bestatin, 1.5 uM E-64, 2 uM Leupeptin Hemisulfate, 1 uM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 2 mg/ml and then an equal volume of 2X SDS-PAGE sample buffer was added.


  • SDS-Page
  • Western Blot 1:100-1:2000
Application Notes
Ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5 minutes and rapidly cool. If dissociating conditions are desired add reducing agent prior to heating. The recommended loading volume per lane is 10-30 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Store at -70C. Avoid freeze-thaw cycles.
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
No Preservative

Lysate Details for Array

Subcellular Fraction


This nuclear cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for HeLa Lysate (NB800-PC9) (0)

There are no publications for HeLa Lysate (NB800-PC9).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HeLa Lysate (NB800-PC9) (0)

There are no reviews for HeLa Lysate (NB800-PC9). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for HeLa Lysate (NB800-PC9) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional HeLa Products

Array NB800-PC9

Research Areas for HeLa Lysate (NB800-PC9)

Find related products by research area.

Blogs on HeLa.

The Role of LC3 within the Autophagic Pathway
We at Novus Biologicals have a broad antibody database covering the area of autophagy - over 1400 reagents in total. Autophagy is the bulk degradation of cytoplasmic components - literally, self-digestion of the cell. Double-membrane vesicles, called ...  Read full blog post.

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our HeLa Nuclear Cell Lysate and receive a gift card or discount.