HeLa Nuclear Cell Lysate


Western Blot: HeLa Nuclear Cell Lysate [NB800-PC9] - Analysis of Histone H3 (NBP1-30141) uisng HeLa nuclear lysate [NB800-PC9].
SDS-Page: HeLa Nuclear Cell Lysate [NB800-PC9] - Staining of HeLa nuclear lysate using NB800-PC9.

Product Details

Reactivity HuSpecies Glossary
Applications WB, PAGE
1.0 mg/ml

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HeLa Nuclear Cell Lysate Summary



Application Notes
Ready-to-use nuclear extracts are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Nuclear extracts are supplied in denaturing buffer without dissociating agents. Heat nuclear extract to 95C for 5 minutes and rapidly cool. If dissociating conditions are desired add reducing agent prior to heating. The recommended loading volume per lane is 10-30 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8), 10% (v/v) Glycerol
No Preservative
1.0 mg/ml

Lysate Details for Array

Subcellular Fraction


Store at -70C or COLDER. For extended storage, aliquot Nuclear Extract to minimize freeze/thaw cycles.


This nuclear cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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WB Video Protocol

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