HeLa Nuclear Cell Lysate


Western Blot: HeLa Nuclear Cell Lysate [NB800-PC9] - Analysis of Histone H3 (NBP1-30141) uisng HeLa nuclear lysate [NB800-PC9].
SDS-Page: HeLa Nuclear Cell Lysate [NB800-PC9] - Staining of HeLa nuclear lysate using NB800-PC9.

Product Details

Reactivity HuSpecies Glossary
Applications WB, PAGE
1.0 mg/ml

Order Details

HeLa Nuclear Cell Lysate Summary

HeLa cell nuclear extract lysate


Application Notes
Lysates are prepared as positive controls for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Heat lysate to 95 degrees C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior to heating.
The recommended loading volume per lane is 0.01-0.03 ml depending on the size format of your gel.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
1X SDS-PAGE Sample Buffer [62.5 mM Tris HCl, 2% SDS, 10% Glycerol, 0.005% bromophenol blue, pH 6.8]
No Preservative
1.0 mg/ml

Lysate Details for Array

Subcellular Fraction


This nuclear cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Array NB800-PC9

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