Laminin Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, In, Rb, ShSpecies Glossary
Applications WB, ICC/IF, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Laminin Antibody [Biotin] Summary

Immunogen
Laminin isolated from mouse EHS tumor. [UniProt# P19137]
Localization
Secreted, Basement membrane, Extracellular matrix
Marker
Basement Membrane Marker
Specificity
This is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMA1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
This Laminin antibody can be used for Immunocytochemistry/Immunofluorescence, Functional assays, Immunohistochemistry paraffin and frozen sections, and Western blotting where it detects bands at 200 and 400 kDa. The antibody functionally inhibits Laminin in mouse and rat. It binds to Laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue.
Readout System
Publications
Read Publication using
NB300-144B in the following applications:

  • IHC
    1 publication

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 18214989). Invertebrate reactivity reported in scientific literature (PMID: 28114363).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
IgG purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Laminin Antibody [Biotin]

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • S-LAM alpha
  • S-laminin subunit alpha

Background

Laminin antibodies are widely used to label blood vessels and basement membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Laminin Antibody (NB300-144B)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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IHC
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Laminin Antibody (NB300-144B) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

DyLight 488 Labeled NB300-144G
DyLight 550 Labeled NB300-144R
DyLight 650 Labeled NB300-144C
HRP Labeled NB300-144H

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Blogs on Laminin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Bioinformatics

Gene Symbol LAMA1