Cytochrome c Antibody (7H8.2C12) - BSA Free


Biological Strategies: Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 more
Immunocytochemistry/ Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - p73 was detected in immersion fixed Hela human cell line using NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells more
Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog more
Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control more
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Human HeLa lysate probed with Cytochrome C antibody at 0.1 ug/ml.
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 ug of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is more
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Detection of Cytochrome C in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Cytochrome C Monoclonal Antibody more
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 more
Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) more
Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C Antibodies (NB100-56503SS), ProteinSimple Western Blot on Jess Instrument; 1 microgram of human brain tissue lysate was tested with the more

Product Details

Reactivity Hu, Mu, Rt, Dr, RbSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, WB
BSA Free
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

Cytochrome c Antibody (7H8.2C12) - BSA Free Summary

Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
Mitochondria Marker
This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
IgG2b Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow (Cell Surface) reported in scientific literature (PMID 16467206)
  • Flow (Intracellular) reported in scientific literature (Mohr et al (2004))
  • Flow Cytometry 1:20-1:2000
  • Immunoblotting reported in scientific literature (PMID 16436379)
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500. Use reported in scientific literature (Yamasaki et al (2006))
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500. Use reported in scientific literature (Fujimara et al (1998))
  • Simple Western 2.5 ug/ml
  • Western Blot 0.05-0.5 ug/ml
Application Notes
An approx. 15 kDa band is observed. Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:50. Matrix was 12-230 kDa.
HeLa Whole Cell Lysate
Cytochrome c Overexpression Lysate
Reviewed Applications
Read 1 Review rated 5
NB100-56503 in the following applications:

Read Publications using
NB100-56503 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for Cytochrome c Antibody (7H8.2C12) - BSA Free

  • CYCS
  • Cytochrome c
  • cytochrome c, somatic
  • THC4


In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytochrome c Antibody (NB100-56503)(60)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Drosophila, Rabbit.

We have publications tested in 5 applications: FLOW, Flow Cytometry Control, Flow-CS, ICC/IF, WB.

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Flow Cytometry Control
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Showing Publications 1 - 10 of 60. Show All 60 Publications.
Publications using NB100-56503 Applications Species
Li S, Chen X, Guan S et al. Precisely Amplifying Intracellular Oxidative Storm by Metal-Organic Coordination Polymers to Augment Anticancer Immunity ACS nano 2023-07-25 [PMID: 37490051]
Oikawa R, Watanabe Y, Yotsuyanagi H et al. DNA methylation at hepatitis B virus integrants and flanking host mitochondrially encoded cytochrome C oxidase III Oncology letters 2022-12-01 [PMID: 36284651] (WB, Human) WB Human
Wang Y, Liao J, Chen K Et Al. Lumbrokinase regulates endoplasmic reticulum stress through IRE1 signaling to improve neurological deficits in ischemic stroke Research Square 2021-07-08 [PMID: 36223864]
Zhao S, Chen W, Li W et al. LncRNA TUG1 attenuates ischaemia-reperfusion-induced apoptosis of renal tubular epithelial cells by sponging miR-144-3p via targeting Nrf2 Journal of cellular and molecular medicine 2021-09-21 [PMID: 34547172] (WB, Mouse) WB Mouse
Hu F, Wu Y, Liu C Et al. Penicillin disrupts mitochondrial function and induces autophagy in colorectal cancer cell lines Oncology letters 2021-10-01 [PMID: 34457046] (WB, Human) WB Human
Gascon S, Jimenez-Moreno N, Jimenez S et al. Nutraceutical composition of three pine bark extracts and their antiproliferative effect on Caco-2 cells Journal of Functional Foods 2019-01-01 (FLOW, Human)

Citation using the PE format of this antibody.
FLOW Human
Liu Z, Liu Q, Zhang B et al. Blood-Brain Barrier Permeable and NO-Releasing Multifunctional Nanoparticles for Alzheimer's Disease Treatment: Targeting NO/cGMP/CREB Signaling Pathways Journal of medicinal chemistry 2021-09-23 [PMID: 34517696]
Deng X, Yang P, Gao T et al. Allicin attenuates myocardial apoptosis, inflammation and mitochondrial injury during hypoxia-reoxygenation: an in vitro study BMC cardiovascular disorders 2021-04-21 [PMID: 33882833]
Nam YW, Kong D, Wang D, et al. Differential modulation of SK channel subtypes by phosphorylation Cell calcium 2021-01-06 [PMID: 33422768] (WB, Mouse) WB Mouse
Yoon JH, Shin JW, Pham TH Methyl lucidone induces apoptosis and G2/M phase arrest via the PI3K/Akt/NF-kappa B pathway in ovarian cancer cells Pharm Biol 2020-12-01 [PMID: 31875458] (WB, Human) WB Human
Show All 60 Publications.

Review for Cytochrome c Antibody (NB100-56503) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-56503:
Filter by Applications
Simple Western
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Images Ratings Applications Species Date Details
Simple Western Cytochrome c NB100-56503
reviewed by:
Verified Customer
Simple Western Human 04/06/2023


ApplicationSimple Western
Sample Testedhuman brain lysate

Product General Protocols

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FAQs for Cytochrome c Antibody (NB100-56503) (0)

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Blogs on Cytochrome c.

Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis
Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

Cytochrome C in Apoptosis, Immune Response and Cancer
Cytochrome C is an electron carrier protein that localizes in mitochondrion intermembrane space and has been identified as one of the key signaling molecules of apoptosis or programmed cell death. Suppression of the anti-apoptotic members or activatio...  Read full blog post.

Mitofilin and the Mitochondrial Inner Membrane Organizing System (MINOS)
Mitofilin was originally described as a heart muscle protein due to its high expression in the heart. Recently, analysis of the human heart mitochondrial proteome demonstrated that Mitofilin is one of the most abundant mitochondrial proteins (1). Rese...  Read full blog post.

Bax Research Gives New Insight into Oxidative Apoptosis
Bax is a member of the Bcl-2 family; an extensive range of proteins which play key roles in apoptosis, or programmed cell death, by regulating outer mitochondrial membrane permeability. We at Novus Biologicals are one of the leading antibody suppliers...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

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Recent Reviews


Verified Customer
Application: Simple Western
Species: Human


Gene Symbol CYCS