Cytochrome c Antibody (7H8.2C12) - BSA Free


Biological Strategies: Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 more
Immunocytochemistry/ Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - p73 was detected in immersion fixed Hela human cell line using NB100-56503 at 25 ug/ml for 3 hours at room temperature. Cells more
Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog more
Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control more
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Human HeLa lysate probed with Cytochrome C antibody at 0.1 ug/ml.
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 ug of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is more
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Detection of Cytochrome C in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Cytochrome C Monoclonal Antibody more
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 more
Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa (as indicated) more

Product Details

Reactivity Hu, Mu, Rt, Dr, RbSpecies Glossary
Applications WB, Simple Western, Flow, Flow-CS, Flow-IC, IB, ICC/IF, IHC, IHC-P
BSA Free
1.0 mg/ml
Validated by:

Biological Strategies


Order Details

Cytochrome c Antibody (7H8.2C12) - BSA Free Summary

Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
Mitochondria Marker
An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
IgG2b Kappa
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow (Cell Surface)
  • Flow (Intracellular)
  • Flow Cytometry 1:20-1:2000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Simple Western 2.5 ug/ml
  • Western Blot 0.05-0.5 ug/ml
Application Notes
Useful in Flow Cytometry (Intracellular): See Mohr (2004) et al. ICC/IF: See Yamasaki (2006) et al. Immunohistochemistry-Paraffin: See Fujimara (1998). et al. Use in Flow-cell surface reported in scientific literature (PMID: 16467206). Use in immunoblotting reported in scientific literature (PMID: 16436379).
Simple Western reported by an internal validation. Separated by Size-Jess/Wes, Sally Sue/Peggy Sue, antibody dilution of 1:50. Apparent MW in kDa on Simple Western was 45kDa; matrix was 12-230 kDa.
HeLa Whole Cell Lysate
Cytochrome c Overexpression Lysate
Read Publications using
NB100-56503 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for Cytochrome c Antibody (7H8.2C12) - BSA Free

  • CYCS
  • Cytochrome c
  • cytochrome c, somatic
  • THC4


In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytochrome c Antibody (NB100-56503)(58)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Drosophila, Rabbit.

We have publications tested in 5 applications: Flow, Flow-Cs, Flow-IC, ICC/IF, WB.

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Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using NB100-56503 Applications Species
Liu Z, Liu Q, Zhang B et al. Blood-Brain Barrier Permeable and NO-Releasing Multifunctional Nanoparticles for Alzheimer's Disease Treatment: Targeting NO/cGMP/CREB Signaling Pathways Journal of medicinal chemistry Sep 23 2021 [PMID: 34517696]
Saladini S, Aventaggiato M, Barreca F et al. Metformin Impairs Glutamine Metabolism and Autophagy in Tumour Cells. Cells Jan 14 2019 [PMID: 30646605] (WB, Human) WB Human
Zhao S, Li W, Cheng F et al. High-pressure carbon dioxide pneumoperitoneum induces oxidative stress and mitochondria-associated apoptotic pathway in rabbit kidneys with severe hydronephrosis. Int J Mol Med 2019 Jan [PMID: 30431064] (Rabbit) Rabbit
Pennington ER, Sullivan EM, Fix A et al. Proteolipid domains form in biomimetic and cardiac mitochondrial vesicles and are regulated by cardiolipin concentration but not monolyso-cardiolipin. J. Biol. Chem. Aug 29 2018 [PMID: 30158245] (ICC/IF, Mouse) ICC/IF Mouse
Waghela BN, Sharma A, Dhumale S et al. Curcumin Conjugated with PLGA Potentiates Sustainability, Anti-Proliferative Activity and Apoptosis in Human Colon Carcinoma Cells PLoS ONE. 2015 Feb 19 [PMID: 25692854] (WB, Human)

Cytochrome c antibody used for WB analysis of lysates from HCT 116 cells treated or not with curcumin and curcumin-PLGA conjugate conjugates for 6, 12 and 24 hours (Fig 8C).
WB Human
Nogami K, Takahama K, Okushima A et al. Tailor-Made Designer Helical Peptides that Induce Mitochondrion-Mediated Cell Death without Necrosis. Chembiochem. 2014 Sep 18 [PMID: 25234153] (WB, Human) WB Human
Sagar S, Kumar P, Behera RR, Pal A. Effects of CEES and LPS synergistically stimulate oxidative stress inactivates OGG1 signaling in macrophage cells. J. Hazard. Mater. 2014 Jun 06 [PMID: 24976129] (WB, Mouse)

Fig 3: RAW264.7
WB Mouse
Kumar P, Rao GN, Pal BB, Pal A. Hyperglycemia-induced oxidative stress induces apoptosis by inhibiting PI3-kinase/Akt and ERK1/2 MAPK mediated signaling pathway causing downregulation of 8-oxoG-DNA glycosylase levels in glial cells. Int. J. Biochem. Cell Biol. 2014 Jun 04 [PMID: 24907397] (WB, Rat)

Fig 4E: primary astroglial cells.Cytochrome C was detected at ~17 kDa.
WB Rat
Wang J, Schilling JM, Niesman IR et al. Cardioprotective Trafficking of Caveolin to Mitochondria Is Gi-protein Dependent. Anesthesiology. 2014 May 12 [PMID: 24821070] (WB, Mouse)

Whole-heart homogenate and isolate heart mitochondria, Fig 5A.
WB Mouse
Csak T, Dolganiuc A, Kodys K et al. Mitochondrial antiviral signaling protein defect links impaired antiviral response and liver injury in steatohepatitis in mice. Hepatology. 2011 Jun [PMID: 21425308] (WB)

Cytochrome c (IMG-101A), Caspase-8 (IMG-5703), Mitochondrial extraction kit (10082k). WB: Mitochondrial and cytoplasmic extract from C57B16 MCD (methionine-choline deficient) diet fed and non MCD diet fed mice, Fig 5.
Show All 58 Publications.

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Blogs on Cytochrome c.

Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis
Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

Cytochrome C in Apoptosis, Immune Response and Cancer
Cytochrome C is an electron carrier protein that localizes in mitochondrion intermembrane space and has been identified as one of the key signaling molecules of apoptosis or programmed cell death. Suppression of the anti-apoptotic members or activatio...  Read full blog post.

Mitofilin and the Mitochondrial Inner Membrane Organizing System (MINOS)
Mitofilin was originally described as a heart muscle protein due to its high expression in the heart. Recently, analysis of the human heart mitochondrial proteome demonstrated that Mitofilin is one of the most abundant mitochondrial proteins (1). Rese...  Read full blog post.

Bax Research Gives New Insight into Oxidative Apoptosis
Bax is a member of the Bcl-2 family; an extensive range of proteins which play key roles in apoptosis, or programmed cell death, by regulating outer mitochondrial membrane permeability. We at Novus Biologicals are one of the leading antibody suppliers...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

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Gene Symbol CYCS