Cytochrome c Antibody (7H8.2C12)

Images

 
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the Biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 mgs of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is ...read more
Immunocytochemistry/ Immunofluorescence: Cytochrome c Antibody (7H8.2C12) [NB100-56503]
Immunohistochemistry-Paraffin: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Cytochrome C was detected in immersion fixed paraffin-embedded sections of human heart using anti-human mouse monoclonal antibody (Catalog ...read more
Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control ...read more
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using cytochrome C antibody. Human HeLa lysate probed with cytochrome C antibody at 0.1 ug/ml.
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Detection of Cytochrome C in Human HeLa Cell Line by Flow Cytometry. Human HeLa cell line was stained with Mouse Anti- Cytochrome C Monoclonal Antibody ...read more
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 ...read more
Simple Western: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytochrome c at approximately 23 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, RbSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
Clone
7H8.2C12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Cytochrome c Antibody (7H8.2C12) Summary

Immunogen
Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
Marker
Mitochondria Marker
Specificity
An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CYCS
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.05-0.5 ug/ml
  • Simple Western 2.5 ug/ml
  • Flow Cytometry 1:20-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
Useful in Flow Cytometry (Intracellular): See Mohr (2004) et al. Immunocytochemistry/Immunofluorescence: See Yamasaki (2006) et al. Immunohistochemistry-Paraffin: See Fujimara (1998). et al.
Positive Control
HeLa Lysate (NB800-PC1)
Cytochrome c Lysate (NBL1-09662)
Publications
Read Publications using
NB100-56503 in the following applications:

Reactivity Notes

Cross reacts with Honey Bee. Mouse reactivity reported in multiple pieces of scientific literature. Rat reactivity reported in scientific literature (PMID: 12821666)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Cytochrome c Antibody (7H8.2C12)

  • CYCHCS
  • CYCS
  • Cytochrome c
  • cytochrome c, somatic
  • THC4

Background

In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
NBP1-28566
Species: Hu, Mu, Rt, Bv, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP
NB100-56098
Species: Hu, Mu, Ca
Applications: WB, IHC, IHC-P, IP
NB100-56118
Species: Hu, Mu, Rt, Ca, Ge
Applications: IHC, IHC-Fr, IHC-P, IP, Flow-IC
NB100-56104
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NB100-56116
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-27335
Species: Hu
Applications: WB, Flow, CyTOF-ready
NB100-56106
Species: Hu, Mu, Rt, Ca, Ge
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-56311
Species: Hu
Applications: WB, ICC/IF, IHC-P
NB100-56074
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-43728
Species: Hu
Applications: WB, ICC/IF
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP1-32550
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-59438
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF
NBP2-30764
Species: Hu
Applications: IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Supplier Logo

Publications for Cytochrome c Antibody (NB100-56503)(46)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila.

We have publications tested in 4 applications: Flow-Cs, Flow-IC, ICC/IF, WB.


Filter By Application
Flow-Cs
(1)
Flow-IC
(4)
ICC/IF
(3)
WB
(35)
All Applications
Filter By Species
Human
(10)
Mouse
(5)
Rat
(7)
Drosophila
(1)
All Species
Showing Publications 1 - 10 of 46. Show All 46 Publications.
Publications using NB100-56503 Applications Species
Dhumale SS, Waghela BN, Pathak C. Quercetin protects necrotic insult and promotes apoptosis by attenuating the expression of RAGE and its ligand HMGB1 in human breast adenocarcinoma cells. IUBMB Life. 2015 May 01 [PMID: 25983116] (WB, Human)

Details:
Cytochrome c antibody was used at 1:1,000 dilution for WB analysis of lysates from human breast adenocarcinoma cell line MCF-7 cells treated or not with 25 & 50 uM of quercetin for 6-48 hours (FIG 3B).
WB Human
Yamasaki E, A Wada, A Kumatori et al. Helicobacter pylori vacuolating cytotoxin induces activation of the proapoptotic proteins Bax and Bak, leading to cytochrome c release and cell death, independent of vacuolation. J Bio Chem 281:11250-11259. 2006
Colell A, Garcia-Ruiz C, Lluis JM et al. Cholesterol impairs the adenine nucleotide translocator-mediated mitochondrial permeability transition through altered membrane fluidity J Biol Chem (WB, Rat)

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NB100-55775).
WB Rat
Patel N, Garikapati KR, Ramaiah MJ et al. miR-15a/miR-16 induces mitochondrial dependent apoptosis in breast cancer cells by suppressing oncogene BMI1 Life Sci. Sep 2 2016 [PMID: 27596816] (WB, Human) WB Human
Huang X, Huang R, Gou S et al. Combretastatin A-4 Analogue: A Dual-Targeting and Tubulin Inhibitor Containing Antitumor Pt(IV) Moiety with a Unique Mode of Action Bioconjug. Chem. Aug 15 2016 [PMID: 27494235] (WB, Human) WB Human
Waghela BN, Sharma A, Dhumale S et al. Curcumin Conjugated with PLGA Potentiates Sustainability, Anti-Proliferative Activity and Apoptosis in Human Colon Carcinoma Cells PLoS ONE. 2015 Feb 19 [PMID: 25692854] (WB, Human)

Details:
Cytochrome c antibody used for WB analysis of lysates from HCT 116 cells treated or not with curcumin and curcumin-PLGA conjugate conjugates for 6, 12 and 24 hours (Fig 8C).
WB Human
Nogami K, Takahama K, Okushima A et al. Tailor-Made Designer Helical Peptides that Induce Mitochondrion-Mediated Cell Death without Necrosis. Chembiochem. 2014 Sep 18 [PMID: 25234153] (WB, Human) WB Human
Sagar S, Kumar P, Behera RR, Pal A. Effects of CEES and LPS synergistically stimulate oxidative stress inactivates OGG1 signaling in macrophage cells. J. Hazard. Mater. 2014 Jun 06 [PMID: 24976129] (WB, Mouse)

Details:
Fig 3: RAW264.7
WB Mouse
Kumar P, Rao GN, Pal BB, Pal A. Hyperglycemia-induced oxidative stress induces apoptosis by inhibiting PI3-kinase/Akt and ERK1/2 MAPK mediated signaling pathway causing downregulation of 8-oxoG-DNA glycosylase levels in glial cells. Int. J. Biochem. Cell Biol. 2014 Jun 04 [PMID: 24907397] (WB, Rat)

Details:
Fig 4E: primary astroglial cells.Cytochrome C was detected at ~17 kDa.
WB Rat
Wang J, Schilling JM, Niesman IR et al. Cardioprotective Trafficking of Caveolin to Mitochondria Is Gi-protein Dependent. Anesthesiology. 2014 May 12 [PMID: 24821070] (WB, Mouse)

Details:
Whole-heart homogenate and isolate heart mitochondria, Fig 5A.
WB Mouse
Show All 46 Publications.

Reviews for Cytochrome c Antibody (NB100-56503) (0)

There are no reviews for Cytochrome c Antibody (NB100-56503). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytochrome c Antibody (NB100-56503) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56503AF405
Alexa Fluor 488 Labeled NB100-56503AF488
Alexa Fluor 647 Labeled NB100-56503AF647
Alexa Fluor 700 Labeled NB100-56503AF700
Allophycocyanin Labeled NB100-56503APC
Biotin Labeled NB100-55775
DyLight 350 Labeled NB100-56503UV
DyLight 405 Labeled NB100-56503V
DyLight 488 Labeled NB100-56503G
DyLight 550 Labeled NB100-56503R
DyLight 650 Labeled NB100-56503C
DyLight 680 Labeled NB100-56503FR
DyLight 755 Labeled NB100-56503IR
HRP Labeled NB100-56503H
PE Labeled NB100-56503PE
PerCP Labeled NB100-56503PCP

Additional Cytochrome c Products

Bioinformatics Tool for Cytochrome c Antibody (NB100-56503)

Discover related pathways, diseases and genes to Cytochrome c Antibody (NB100-56503). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytochrome c Antibody (NB100-56503)

Discover more about diseases related to Cytochrome c Antibody (NB100-56503).
 

Pathways for Cytochrome c Antibody (NB100-56503)

View related products by pathway.

PTMs for Cytochrome c Antibody (NB100-56503)

Learn more about PTMs related to Cytochrome c Antibody (NB100-56503).
 

Research Areas for Cytochrome c Antibody (NB100-56503)

Find related products by research area.

Blogs on Cytochrome c.

Pathway Highlight: Three key factors that contribute to cellular heterogeneity in apoptosis
Have you ever wondered why cells in the same population respond differently to an apoptotic stimulus? Apoptosis, a form of programmed cell death, is vital for the removal of unwanted or damaged cells. As with most cellular processes, too much or to...  Read full blog post.

The use of apoptosis antibodies and controls in cell death research
Apoptosis is a method of programmed cell death that is notably characterized by a morphological change in cellular nuclei and membrane appearance.  Not to be confused with necrosis, apoptosis is a pathway that is induced by a variety of factors tha...  Read full blog post.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

Cytochrome C in Apoptosis, Immune Response and Cancer
Cytochrome C is an electron carrier protein that localizes in mitochondrion intermembrane space and has been identified as one of the key signaling molecules of apoptosis or programmed cell death. Suppression of the anti-apoptotic members or activatio...  Read full blog post.

Mitofilin and the Mitochondrial Inner Membrane Organizing System (MINOS)
Mitofilin was originally described as a heart muscle protein due to its high expression in the heart. Recently, analysis of the human heart mitochondrial proteome demonstrated that Mitofilin is one of the most abundant mitochondrial proteins (1). Rese...  Read full blog post.

Bax Research Gives New Insight into Oxidative Apoptosis
Bax is a member of the Bcl-2 family; an extensive range of proteins which play key roles in apoptosis, or programmed cell death, by regulating outer mitochondrial membrane permeability. We at Novus Biologicals are one of the leading antibody suppliers...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Cytochrome c Antibody (7H8.2C12) and receive a gift card or discount.

Bioinformatics

Gene Symbol CYCS
Entrez
Uniprot