Cytochrome c Antibody (7H8.2C12)


Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using the Biotin conjugate of NB100-56503. Detection of Cytochrome C in 15 mgs of HeLa cell lysate using NB100-55775 at 1:1000. A 15 kDa band is more
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Analysis using cytochrome C antibody. Human HeLa lysate probed with cytochrome C antibody at 0.1 ug/ml.

Product Details

Reactivity Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, RbSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
0.5 mg/ml

Cytochrome c Antibody (7H8.2C12) Summary

Synthetic peptides corresponding to amino acids 1-80, 81-104 and 66-104 of pigeon CYT were used as the immunogen (Jemmerson et al. 1991). The antibody recognizes an epitope within amino acids 93-104 of pigeon cytochrome C based on competitive ELISA results (Jemmerson et al. 1991).
Mitochondria Marker
An approx. 15 kDa band is observed. This antibody recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
IgG2b Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS containing 0.5% BSA
0.05% Sodium Azide
0.5 mg/ml
Protein G purified


Application Notes
Useful in Flow Cytometry (Intracellular): See Mohr (2004) et al. Immunocytochemistry/Immunofluorescence: See Yamasaki (2006) et al. Immunohistochemistry-Paraffin: See Fujimara (1998). et al.
Read Publications using
NB100-56503 in the following applications:

Reactivity Notes

Cross reacts with Honey Bee. Mouse reactivity reported in multiple pieces of scientific literature. Rat reactivity reported in scientific literature (PMID: 12821666)

Alternate Names for Cytochrome c Antibody (7H8.2C12)

  • CYCS
  • cytochrome c
  • cytochrome c, somatic
  • THC4


In addition to its well-known role in electron transport, cytochrome c (CYT) has been identified as a mediator of apoptosis or programmed cell death. Cytochrome c translocates from mitochondria into the cytoplasm in the early phases of apoptosis and plays a role in initiation of the apoptotic proteolytic cascade by activating caspase-3. Cytochrome c has been shown to bind Apaf-1. Apaf-1 has also been shown to activate capase-3.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
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Species: Hu
Applications: WB, ICC/IF, IHC-P
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Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
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Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC, ICFlow
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Publications for Cytochrome c Antibody (NB100-56503)(44)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Drosophila.

We have publications tested in 4 applications: Flow-Cs, Flow-IC, ICC/IF, WB.

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Showing Publications 1 - 10 of 44. Show All 44 Publications.
Publications using NB100-56503 Applications Species
Dhumale SS, Waghela BN, Pathak C. Quercetin protects necrotic insult and promotes apoptosis by attenuating the expression of RAGE and its ligand HMGB1 in human breast adenocarcinoma cells. IUBMB Life. 2015 May 01 [PMID:25983116] (WB, Human) WB Human
Waghela BN, Sharma A, Dhumale S et al. Curcumin Conjugated with PLGA Potentiates Sustainability, Anti-Proliferative Activity and Apoptosis in Human Colon Carcinoma Cells PLoS ONE. 2015 Feb 19 [PMID:25692854] (WB, Human) WB Human
Nogami K, Takahama K, Okushima A et al. Tailor-Made Designer Helical Peptides that Induce Mitochondrion-Mediated Cell Death without Necrosis. Chembiochem. 2014 Sep 18 [PMID:25234153] (WB, Human) WB Human
Sagar S, Kumar P, Behera RR, Pal A. Effects of CEES and LPS synergistically stimulate oxidative stress inactivates OGG1 signaling in macrophage cells. J. Hazard. Mater. 2014 Jun 06 [PMID:24976129] (WB, Mouse) WB Mouse
Kumar P, Rao GN, Pal BB, Pal A. Hyperglycemia-induced oxidative stress induces apoptosis by inhibiting PI3-kinase/Akt and ERK1/2 MAPK mediated signaling pathway causing downregulation of 8-oxoG-DNA glycosylase levels in glial cells. Int. J. Biochem. Cell Biol. 2014 Jun 04 [PMID:24907397] (WB, Rat) WB Rat
Wang J, Schilling JM, Niesman IR et al. Cardioprotective Trafficking of Caveolin to Mitochondria Is Gi-protein Dependent. Anesthesiology. 2014 May 12 [PMID:24821070] (WB, Mouse) WB Mouse
Pradhan N, Pratheek BM, Garai A et al. Induction of apoptosis by Fe(salen)Cl through caspase-dependent pathway specifically in tumor cells. Cell Biol. Int. 2014 May 07 [PMID:24804954] (WB, Human, Mouse) WB Human, Mouse
Zhang W, Wang X, Chen T. Resveratrol induces apoptosis via a Bak-mediated intrinsic pathway in human lung adenocarcinoma cells Cellular Signalling 41041 [PMID:22245142] (WB, Human) WB Human
Csak T, Dolganiuc A, Kodys K et al. Mitochondrial antiviral signaling protein defect links impaired antiviral response and liver injury in steatohepatitis in mice. Hepatology. 2011 Jun [PMID:21425308] (WB) WB
Zall H, Weber A, Besch R et al. Chemotherapeutic drugs sensitize human renal cell carcinoma cells to ABT-737 by a mechanism involving the Noxa-dependent inactivation of Mcl-1 or A1. Mol Cancer. 2010 Jun 24 [PMID:20576107] (WB) WB
Show All 44 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytochrome c Antibody (NB100-56503) (0)

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Positive Control Lysate(s)

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB100-56503AF405
Alexa Fluor (R) 488 Labeled NB100-56503AF488
Alexa Fluor (R) 647 Labeled NB100-56503AF647
Alexa Fluor (R) 700 Labeled NB100-56503AF700
Allophycocyanin Labeled NB100-56503APC
Biotin Labeled NB100-55775
DyLight 350 Labeled NB100-56503UV
DyLight 405 Labeled NB100-56503V
DyLight 405LS Labeled NB100-56503V3
DyLight 488 Labeled NB100-56503G
DyLight 550 Labeled NB100-56503R
DyLight 650 Labeled NB100-56503C
DyLight 680 Labeled NB100-56503FR
DyLight 755 Labeled NB100-56503IR
HRP Labeled NB100-56503H
PE Labeled NB100-56503PE
PerCP Labeled NB100-56503PCP

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Blogs on Cytochrome c.

Bcl-2 - an antiapoptotic protein with an important role in cancer cell survival
B-cell lymphoma 2 (Bcl-2) protein is an oncogene that normally acts as an apoptotic inhibitor and localizes to the mitochondrial membrane where it prevents the release of cytochrome c. The Bcl-2 protein family consists of over 20 proteins each co...  Read full blog post.

Cytochrome C - a mediator of apoptosis
Cytochrome C is a small heme protein within the inner mitochondrial membrane responsible for carrying electrons within the respiratory transport chain.  Additionally, cytochrome c has also been identified as a player in programmed cell death (apop...  Read full blog post.

Cytochrome C in Apoptosis, Immune Response and Cancer
Cytochrome C is an electron carrier protein that localizes in mitochondrion intermembrane space and has been identified as one of the key signaling molecules of apoptosis or programmed cell death. Suppression of the anti-apoptotic members or activatio...  Read full blog post.

Mitofilin and the Mitochondrial Inner Membrane Organizing System (MINOS)
Mitofilin was originally described as a heart muscle protein due to its high expression in the heart. Recently, analysis of the human heart mitochondrial proteome demonstrated that Mitofilin is one of the most abundant mitochondrial proteins (1). Rese...  Read full blog post.

Bax Research Gives New Insight into Oxidative Apoptosis
Bax is a member of the Bcl-2 family; an extensive range of proteins which play key roles in apoptosis, or programmed cell death, by regulating outer mitochondrial membrane permeability. We at Novus Biologicals are one of the leading antibody suppliers...  Read full blog post.

The Role of the Caspase 3 Antibody in Apoptosis Research
The caspases are a group of cysteine protease enzymes essential to apoptosis, inflammation and necrosis. Caspase 3 has been identified as one of the key mediators of apoptosis.Human caspases form three distinct groups: cytokine activators, which are...  Read full blog post.

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Product PDFs


Gene Symbol CYCS

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