Recombinant Human EGF Protein, CF

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1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human EGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL.
The specific activity of Recombinant Human EGF is approximately 1.4 x 103 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
Source
E. coli-derived human EGF protein
Asn971-Arg1023, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
EGF
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
6 kDa, reducing conditions
Publications
Read Publications using
236-EG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EGF Protein, CF

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of The EGF family are characterized by a shared structural motif, the EGF‑like domain, which contains three intramolecular disulfide bonds that are formed by six similarly spaced, conserved cysteine residues (2). These disulfide bonds are essential for proper protein conformation and receptor binding. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The full length EGF protein is 1207 amino acids (aa) (EGF precursor) containing nine EGF domains and nine LDLR class B repeats. However, the mature protein is much smaller, only 53 aa, and is generated by proteolytic cleavage of the EGF domain proximal to the transmembrane region (3). EGF is well conserved across mammals with mature human EGF 70% identical to mature mouse and rat EGF. Physiologically, EGF is found in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

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Publications for EGF (236-EG)(452)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Canine, Chicken, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Xenograft.

We have publications tested in 7 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Organoid Culture, Stimulation, Tissue Culture.


Filter By Application
Bioassay
(398)
Cell Culture
(29)
Differentiation
(2)
In Vivo
(8)
Organoid Culture
(1)
Stimulation
(1)
Tissue Culture
(2)
All Applications
Filter By Species
Human
(389)
Mouse
(34)
Rat
(8)
Canine
(3)
Chicken
(3)
Porcine
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Xenograft
(1)
All Species
Showing Publications 1 - 10 of 452. Show All 452 Publications.
Publications using 236-EG Applications Species
J Pan, LX Zhao Epithelial-mesenchymal transition suppresses ERas to activate autophagy in retinal pigment epithelial cells in proliferative vitreoretinopathy Eur Rev Med Pharmacol Sci, 2020;24(9):4627-4634. 2020 [PMID: 32432726] (Cell Culture, Human) Cell Culture Human
AR Nickolls, MM Lee, DF Espinoza, M Szczot, RM Lam, Q Wang, J Beers, J Zou, MQ Nguyen, HJ Solinski, AA AlJanahi, KR Johnson, ME Ward, AT Chesler, CG Bönnemann Transcriptional Programming of Human Mechanosensory Neuron Subtypes from Pluripotent Stem Cells Cell Rep, 2020;30(3):932-946.e7. 2020 [PMID: 31968264] (Differentiation, Human) Differentiation Human
S Yomtoubian, SB Lee, A Verma, F Izzo, G Markowitz, H Choi, L Cerchietti, L Vahdat, KA Brown, E Andreopoul, O Elemento, J Chang, G Inghirami, D Gao, S Ryu, V Mittal Inhibition of EZH2 Catalytic Activity Selectively Targets a Metastatic Subpopulation in Triple-Negative Breast Cancer Cell Rep, 2020;30(3):755-770.e6. 2020 [PMID: 31968251] (Cell Culture, Mouse) Cell Culture Mouse
L Cai, SV Kirchleitn, D Zhao, M Li, JC Tonn, R Glass, RE Kälin Glioblastoma Exhibits Inter-Individual Heterogeneity of TSPO and LAT1 Expression in Neoplastic and Parenchymal Cells Int J Mol Sci, 2020;21(2):. 2020 [PMID: 31963507] (Bioassay, Human) Bioassay Human
H Ramos, J Calheiros, J Almeida, V Barcherini, S Santos, ATP Carvalho, MMM Santos, L Saraiva SLMP53-1 Inhibits Tumor Cell Growth through Regulation of Glucose Metabolism and Angiogenesis in a P53-Dependent Manner Int J Mol Sci, 2020;21(2):. 2020 [PMID: 31963392] (Cell Culture, Human) Cell Culture Human
C Peng, Y Li, L Lu, J Zhu, H Li, J Hu Efficient One-Step Induction of Human Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSCs) Produces MSC-Derived Neurospheres (MSC-NS) with Unique Transcriptional Profile and Enhanced Neurogenic and Angiogenic Secretomes Stem Cells Int, 2019;2019(0):9208173. 2019 [PMID: 31933651] (Cell Culture, Human) Cell Culture Human
M Lundberg, S Curbo, H Bohman, I Agartz, SO Ögren, C Patrone, S Mansouri Clozapine protects adult neural stem cells from ketamine-induced cell death in correlation with decreased apoptosis and autophagy Biosci. Rep., 2020;0(0):. 2020 [PMID: 31919522] (Cell Culture, Mouse) Cell Culture Mouse
É Dóka, T Ida, M Dagnell, Y Abiko, NC Luong, N Balog, T Takata, B Espinosa, A Nishimura, Q Cheng, Y Funato, H Miki, JM Fukuto, JR Prigge, EE Schmidt, ESJ Arnér, Y Kumagai, T Akaike, P Nagy Control of protein function through oxidation and reduction of persulfidated states Sci Adv, 2020;6(1):eaax8358. 2020 [PMID: 31911946] (Cell Culture, Human) Cell Culture Human
K Kawai, R Negoro, M Ichikawa, T Yamashita, S Deguchi, K Harada, K Hirata, K Takayama, H Mizuguchi Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies Mol Ther Methods Clin Dev, 2020;17(0):49-57. 2020 [PMID: 31890740] (Cell Culture, Human) Cell Culture Human
B You, F Mercier, E Assenat, C Langlois-J, O Glehen, J Soulé, L Payen, V Kepenekian, M Dupuy, F Belouin, E Morency, V Saywell, M Flacelière, P Elies, P Liaud, T Mazard, D Maucort-Bo, W Tan, B Vire, L Villeneuve, M Ychou, M Kohli, D Joubert, A Prieur The oncogenic and druggable hPG80 (Progastrin) is overexpressed in multiple cancers and detected in the blood of patients EBioMedicine, 2019;51(0):102574. 2019 [PMID: 31877416] (Bioassay, Human) Bioassay Human
Show All 452 Publications.

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Bioinformatics

Gene Symbol EGF
Uniprot