Recombinant Human EGF Protein, CF

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Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human EGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL.
The specific activity of Recombinant Human EGF is approximately 1.4 x 103 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
Source
E. coli-derived human EGF protein
Asn971-Arg1023, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
EGF
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
6 kDa, reducing conditions
Publications
Read Publications using
236-EG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EGF Protein, CF

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature human EGF shares 70% aa sequence identity with mature mouse and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

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Publications for EGF (236-EG)(317)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 3 applications: Bioassay, In Vivo, cell culture.


Filter By Application
Bioassay
(296)
In Vivo
(8)
cell culture
(4)
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Filter By Species
Human
(266)
Mouse
(25)
Rat
(8)
Canine
(3)
Chicken
(3)
Porcine
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
All Species
Showing Publications 1 - 10 of 317. Show All 317 Publications.
Publications using 236-EG Applications Species
CH Peng, LC Woung, KH Lu, CY Tsai, SD Lee, CS Huang, TC Lin, KH Chien, DK Hwang Acoustic waves improves retroviral transduction in human retinal stem cells J Chin Med Assoc, 2018;0(0):. 2018 [PMID: 29941298] (Bioassay, Human) Bioassay Human
D Garnier, R Li, F Delbos, A Fourrier, C Collet, C Guguen-Gui, C Chesné, TH Nguyen Expansion of human primary hepatocytes in vitro through their amplification as liver progenitors in a 3D organoid system Sci Rep, 2018;8(1):8222. 2018 [PMID: 29844473] (Bioassay, Human) Bioassay Human
MH Choe, Y Yoon, J Kim, SG Hwang, YH Han, JS Kim miR-550a-3-5p acts as a tumor suppressor and reverses BRAF inhibitor resistance through the direct targeting of YAP Cell Death Dis, 2018;9(6):640. 2018 [PMID: 29844307] (Bioassay, Human) Bioassay Human
GS Wong, J Zhou, JB Liu, Z Wu, X Xu, T Li, D Xu, SE Schumacher, J Puschhof, J McFarland, C Zou, A Dulak, L Henderson, P Xu, E O'Day, R Rendak, WL Liao, F Cecchi, T Hembrough, S Schwartz, C Szeto, AK Rustgi, KK Wong, JA Diehl, K Jensen, F Graziano, A Ruzzo, S Fereshetia, P Mertins, SA Carr, R Beroukhim, K Nakamura, E Oki, M Watanabe, H Baba, Y Imamura, D Catenacci, AJ Bass Targeting wild-type KRAS-amplified gastroesophageal cancer through combined MEK and SHP2 inhibition Nat. Med., 2018;0(0):. 2018 [PMID: 29808010] (Bioassay, Human) Bioassay Human
H Yang, C Wang, H Chen, L Li, S Ma, H Wang, Y Fu, T Qu Neural Stem Cell-Conditioned Medium Ameliorated Cerebral Ischemia-Reperfusion Injury in Rats Stem Cells Int, 2018;2018(0):4659159. 2018 [PMID: 29765412] (Bioassay, Human) Bioassay Human
JM Kim, S Choi, SW Lee, K Park Voltage-dependent Ca2+ channels promote branching morphogenesis of salivary glands by patterning differential growth Sci Rep, 2018;8(1):7566. 2018 [PMID: 29765108] (Bioassay, Mouse) Bioassay Mouse
B Roy, S Venkatacha, P Ratna, Y Wang, DS Jokhun, M Nagarajan, GV Shivashank Laterally confined growth of cells induces nuclear reprogramming in the absence of exogenous biochemical factors Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29735717] (Bioassay, Human) Bioassay Human
IG Ryoo, BH Choi, SK Ku, MK Kwak High CD44 expression mediates p62-associated NFE2L2/NRF2 activation in breast cancer stem cell-like cells: Implications for cancer stem cell resistance Redox Biol, 2018;17(0):246-258. 2018 [PMID: 29729523] (Bioassay, Human) Bioassay Human
JR Fan, HT Lee, W Lee, CH Lin, CY Hsu, CH Hsieh, WC Shyu Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model Cell Death Dis, 2018;9(5):502. 2018 [PMID: 29717132] (Bioassay, Human) Bioassay Human
Y Zhang, Q Zhang, Z Cao, Y Huang, S Cheng, D Pang HOXD3 Plays a Critical Role in Breast Cancer Stemness and Drug Resistance Cell. Physiol. Biochem., 2018;46(4):1737-1747. 2018 [PMID: 29698974] (Bioassay, Human) Bioassay Human
Show All 317 Publications.

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Bioinformatics

Gene Symbol EGF
Entrez
Uniprot