Recombinant Human EGF Protein, CF

Images

 
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.
Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human EGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL.
The specific activity of Recombinant Human EGF is approximately 1.4 x 103 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).   Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human EGF protein
Asn971-Arg1023, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
EGF
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
6 kDa, reducing conditions
Publications
Read Publications using
236-EG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EGF Protein, CF

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • hEGF
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone

Background

Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of The EGF family are characterized by a shared structural motif, the EGF‑like domain, which contains three intramolecular disulfide bonds that are formed by six similarly spaced, conserved cysteine residues (2). These disulfide bonds are essential for proper protein conformation and receptor binding. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The full length EGF protein is 1207 amino acids (aa) (EGF precursor) containing nine EGF domains and nine LDLR class B repeats. However, the mature protein is much smaller, only 53 aa, and is generated by proteolytic cleavage of the EGF domain proximal to the transmembrane region (3). EGF is well conserved across mammals with mature human EGF 70% identical to mature mouse and rat EGF. Physiologically, EGF is found in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

Customers Who Viewed This Item Also Viewed...

AF231
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, ICC, IHC, IP, WB
233-FB/CF
Species: Hu
Applications: BA
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP2-67360
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-22203
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
D6050
Species: Hu
Applications: ELISA
1129-ER
Species: Hu
Applications: BA
294-HG/CF
Species: Hu
Applications: BA
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
AF467
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
DVE00
Species: Hu
Applications: ELISA
239-A
Species: Hu
Applications: BA
NBP2-67471
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB

Publications for EGF (236-EG)(545)

We have publications tested in 10 confirmed species: Human, Mouse, Rat, Canine, Chicken, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Transgenic Mouse, Xenograft, Zebrafish.

We have publications tested in 7 applications: Bioassay, Cell Culture, Differentiation, In Vivo, Organoid Culture, Stimulation, Tissue Culture.


Filter By Application
Bioassay
(462)
Cell Culture
(57)
Differentiation
(2)
In Vivo
(8)
Organoid Culture
(1)
Stimulation
(1)
Tissue Culture
(2)
All Applications
Filter By Species
Human
(475)
Mouse
(39)
Rat
(8)
Canine
(3)
Chicken
(3)
Porcine
(2)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Transgenic Mouse
(1)
Xenograft
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 10 of 545. Show All 545 Publications.
Publications using 236-EG Applications Species
X Morales, R Peláez, S Garasa, C Ortiz de S, A Rouzaut CRMP2 as a Candidate Target to Interfere with Lung Cancer Cell Migration Biomolecules, 2021;11(10):. 2021 [PMID: 34680167] (Bioassay, Human) Bioassay Human
A Tarallo, C Damiano, S Strollo, N Minopoli, A Indrieri, E Polishchuk, F Zappa, E Nusco, S Fecarotta, C Porto, M Coletta, R Iacono, M Moracci, R Polishchuk, DL Medina, P Imbimbo, DM Monti, MA De Matteis, G Parenti Correction of oxidative stress enhances enzyme replacement therapy in Pompe disease Embo Molecular Medicine, 2021;0(0):e14434. 2021 [PMID: 34606154] (Bioassay, Human) Bioassay Human
T Miyake, M Kuge, Y Matsumoto, M Shimada alpha-glucosyl-rutin activates immediate early genes in human induced pluripotent stem cells Stem Cell Research, 2021;56(0):102511. 2021 [PMID: 34455240] (Bioassay, Human) Bioassay Human
AV Parent, G Faleo, J Chavez, M Saxton, DI Berrios, NR Kerper, Q Tang, M Hebrok Selective deletion of human leukocyte antigens protects stem cell-derived islets from immune rejection Cell Reports, 2021;36(7):109538. 2021 [PMID: 34407395] (Bioassay, Human) Bioassay Human
IT Stancil, JE Michalski, D Davis-Hall, HW Chu, JA Park, CM Magin, IV Yang, BJ Smith, E Dobrinskik, DA Schwartz Pulmonary fibrosis distal airway epithelia are dynamically and structurally dysfunctional Nature Communications, 2021;12(1):4566. 2021 [PMID: 34315881] (Bioassay, Human) Bioassay Human
MO Lee, SG Lee, CR Jung, YS Son, JW Ryu, KB Jung, JH Ahn, JH Oh, HA Lee, JH Lim, J Kim, I Jang, J Choi, J Jung, K Park, B Lee, DS Kim, MY Son, HS Cho Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells Nature Communications, 2021;12(1):4492. 2021 [PMID: 34301945] (Bioassay, Human) Bioassay Human
J Barra, J Cerda-Infa, L Sandoval, P Gajardo-Me, JF Henriquez, M Labarca, C Metz, J Venegas, C Retamal, C Oyanadel, J Cancino, A Soza, MA Cuello, JC Roa, VP Montecinos, A Gonzalez D-Propranolol Impairs EGFR Trafficking and Destabilizes Mutant p53 Counteracting AKT Signaling and Tumor Malignancy Cancers, 2021;13(14):. 2021 [PMID: 34298835] (Bioassay, Human) Bioassay Human
K Jamal Jame, WJ Gallert, SD Yanik, S Panek, J Kronsbein, D Jungck, A Koch, J Knobloch Biomarkers for Comorbidities Modulate the Activity of T-Cells in COPD International Journal of Molecular Sciences, 2021;22(13):. 2021 [PMID: 34281240] (Bioassay, Human) Bioassay Human
Y Andersson, KG Fleten, TW Abrahamsen, W Reed, B Davidson, K Flatmark Anti-Angiogenic Treatment in Pseudomyxoma Peritonei-Still a Strong Preclinical Rationale Cancers, 2021;13(11):. 2021 [PMID: 34198773] (Bioassay, Human) Bioassay Human
J Dong, J Li, Y Li, Z Ma, Y Yu, CY Wang Transcriptional super-enhancers control cancer stemness and metastasis genes in squamous cell carcinoma Nature Communications, 2021;12(1):3974. 2021 [PMID: 34172737] (Bioassay, Human) Bioassay Human
Show All 545 Publications.

Reviews for EGF (236-EG) (0)

There are no reviews for EGF (236-EG). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for EGF (236-EG) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional EGF Products

Bioinformatics Tool for EGF (236-EG)

Discover related pathways, diseases and genes to EGF (236-EG). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EGF (236-EG)

Discover more about diseases related to EGF (236-EG).
 

Pathways for EGF (236-EG)

View related products by pathway.

PTMs for EGF (236-EG)

Learn more about PTMs related to EGF (236-EG).

Blogs on EGF.

Using EGF Protein from Novus Biologicals
EGF (epidermal growth factor) stimulates differentiation, proliferation and cell growth by binding to its receptor, EGFR. EGF was first discovered in the mouse submandibular gland in 1986 by Stanley Cohen of Vanderbilt University, leading to a Nobel P...  Read full blog post.

Myosin is More than Just a Heavy Lifter
Myosin is a well-known, hexameric molecular motor that is a key cytoskeletal component. It consists of a pair of myosin heavy chain subunits (MHC), a pair of essential myosin light chain subunits (MLC), and a pair of regulatory light chain subunits (R...  Read full blog post.

Customers Who Bought This Also Bought

EGF [HRP]
DEG00

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human EGF Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol EGF
Uniprot