Recombinant Human EGF Protein, CF


Recombinant Human EGF (Catalog # 236‑EG) stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.
1 µg/lane of Recombinant Human EGF was resolved with SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human EGF Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL.
The specific activity of Recombinant Human EGF is approximately 1.4 x 103 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
E. coli-derived human EGF protein
Asn971-Arg1023, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
6 kDa, reducing conditions
Read Publications using
236-EG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human EGF Protein, CF

  • beta-urogastrone
  • EGF
  • epidermal growth factor (beta-urogastrone)
  • epidermal growth factor
  • HOMG4
  • pro-epidermal growth factor
  • URG
  • Urogastrone


Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature human EGF shares 70% aa sequence identity with mature mouse and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
  1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
  2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
  3. Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.
  4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
  5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
  6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
  7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
  8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.

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Publications for EGF (236-EG)(378)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Chicken, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque).

We have publications tested in 5 applications: Bioassay, Cell Culture Treatment, In Vivo, Organoid Culture, cell culture.

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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
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Showing Publications 1 - 10 of 378. Show All 378 Publications.
Publications using 236-EG Applications Species
A Hall, K Choi, W Liu, J Rose, C Zhao, Y Yu, Y Na, Y Cai, RA Coover, Y Lin, E Dombi, M Kim, D Levanon, Y Groner, E Boscolo, D Pan, PP Liu, QR Lu, N Ratner, G Huang, J Wu RUNX represses Pmp22 to drive neurofibromagenesis Sci Adv, 2019;5(4):eaau8389. 2019 [PMID: 31032403] (Bioassay, Mouse) Bioassay Mouse
M Matsushita, Y Mori, K Uchiumi, T Ogata, M Nakamura, H Yoda, H Soda, N Takiguchi, Y Nabeya, O Shimozato, T Ozaki PTPRK suppresses progression and chemo-resistance of colon cancer cells via direct inhibition of pro-oncogenic CD133 FEBS Open Bio, 2019;0(0):. 2019 [PMID: 30947381] (Bioassay, Human) Bioassay Human
HL Zhang, P Wang, MZ Lu, SD Zhang, L Zheng c-Myc maintains the self-renewal and chemoresistance properties of colon cancer stem cells Oncol Lett, 2019;17(5):4487-4493. 2019 [PMID: 30944638] (Bioassay, Human) Bioassay Human
Z Shen, C Zhang, L Qu, C Lu, M Xiao, R Ni, J Liu MKP-4 suppresses hepatocarcinogenesis by targeting ERK1/2 pathway Cancer Cell Int., 2019;19(0):61. 2019 [PMID: 30923463] (Bioassay, Human) Bioassay Human
Z Wang, L Kang, H Zhang, Y Huang, L Fang, M Li, PJ Brown, CH Arrowsmith, J Li, J Wong AKT drives SOX2 overexpression and cancer cell stemness in esophageal cancer by protecting SOX2 from UBR5-mediated degradation Oncogene, 2019;0(0):. 2019 [PMID: 30894683] (Bioassay, Human) Bioassay Human
A Linkous, D Balamatsia, M Snuderl, L Edwards, K Miyaguchi, T Milner, B Reich, L Cohen-Goul, A Storaska, Y Nakayama, E Schenkein, R Singhania, S Cirigliano, T Magdeldin, Y Lin, G Nanjangud, K Chadalavad, D Pisapia, C Liston, HA Fine Modeling Patient-Derived Glioblastoma with Cerebral Organoids Cell Rep, 2019;26(12):3203-3211.e5. 2019 [PMID: 30893594] (Bioassay, Human) Bioassay Human
MA Ajmone-Cat, A Onori, C Toselli, E Stronati, M Morlando, I Bozzoni, E Monni, Z Kokaia, G Lupo, L Minghetti, S Biagioni, E Cacci Increased FUS levels in astrocytes leads to astrocyte and microglia activation and neuronal death Sci Rep, 2019;9(1):4572. 2019 [PMID: 30872738] (Bioassay, Human) Bioassay Human
N Naldaiz-Ga, M Goicoechea, IM Aragón, V Pérez-Lópe, S Fuertes-Al, B Herrera-Im, A López de M, R de Luna-Di, PM Baptista, MA Fernández, MF Lara, A Izeta Isolation and characterization of myogenic precursor cells from human cremaster muscle Sci Rep, 2019;9(1):3454. 2019 [PMID: 30837559] (Bioassay, Human) Bioassay Human
YP Xu, Y Qiu, B Zhang, G Chen, Q Chen, M Wang, F Mo, J Xu, J Wu, RR Zhang, ML Cheng, NN Zhang, B Lyu, WL Zhu, MH Wu, Q Ye, D Zhang, JH Man, XF Li, J Cui, Z Xu, B Hu, X Zhou, CF Qin Zika virus infection induces RNAi-mediated antiviral immunity in human neural progenitors and brain organoids Cell Res., 2019;0(0):. 2019 [PMID: 30814679] (Bioassay, Human) Bioassay Human
J Klose, S Trefz, T Wagner, L Steffen, A Prei beta endör, P Radhakrish, C Volz, T Schmidt, A Ulrich, SM Dieter, C Ball, H Glimm, M Schneider Salinomycin: Anti-tumor activity in a pre-clinical colorectal cancer model PLoS ONE, 2019;14(2):e0211916. 2019 [PMID: 30763370] (Bioassay, Human) Bioassay Human
Show All 378 Publications.

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Gene Symbol EGF