Laminin Antibody

Images

 
Immunohistochemistry Free-Floating: Laminin Antibody [NB300-144] - Immunohistological analysis of brain stem section stained with rabbit pAb to laminin, NB300-144, dilution 1:1,000 in red, and costained with chicken pAb ...read more
Immunohistochemistry: Laminin Antibody [NB300-144] - MxIF analysis of mouse submandibular salivary gland morphogenesis. MxIF of a developmental TMA including embryonic stages (E14, E16, E18) and postnatal stages (P5 and ...read more
Immunohistochemistry Free-Floating: Laminin Antibody [NB300-144] - Staining of mouse section of cortex stained with Laminin- (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement ...read more
Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using NB 300-144. Pepsin antigen retrieval was performed on this tissue sample.
Western Blot: Laminin Antibody [NB300-144] - Analysis of Laminin-1 expression in mouse EHS tumor crude extracts (left) and Laminin-2 expression in rat heart crude extracts (right). The Laminin polyclonal antibody was ...read more
Immunocytochemistry/ Immunofluorescence: Laminin Antibody [NB300-144] - IF Confocal analysis of HeLa cells using Laminin antibody (NB300-144, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Western Blot: Laminin Antibody [NB300-144] - Analysis of rat heart cells lysates (lane 1) and 0.2 ug of purified laminin protein from mouse EHS sarcoma (lane 2) This antibody recognizes 3 laminin isotypes: alpha 1 ...read more
Immunocytochemistry/ Immunofluorescence: Laminin Antibody [NB300-144] - Detection of Laminin (Green) in Hela cells using NB300-144 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Flow Cytometry: Laminin Antibody [NB300-144] - A surface stain was performed on HeLa cells with Laminin Antibody NB300-144AF647 (blue) and a matched isotype control (orange). Cells were incubated in an antibody ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHa, In, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Laminin Antibody Summary

Immunogen
Laminin 111 isolated from mouse Engelbreth-Holm-Swarm (EHS) sarcoma cells. [UniProt# P19137]
Localization
Secreted, Basement membrane, Extracellular matrix
Marker
Basement Membrane Marker
Specificity
This is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMA1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:5000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Frozen 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunohistochemistry Free-Floating 1:1000-1:5000
Application Notes
This Laminin antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry, and Western blotting where it detects bands at around 440, 220, and 158kDa. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue. Use in IHC-P and IHC-Fr reported in scientific literature (PMID: 31153978 and 30367035 respectively).

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Laminin Knockout HeLa Cell Lysate
Reviewed Applications
Read 7 Reviews rated 4.4
using
NB300-144 in the following applications:

Publications
Read Publications using
NB300-144 in the following applications:

Reactivity Notes

Rabbit, Sheep, Chinese Hamster, and Invertebrate reactivity reported in scientific literature (PMID: 18214989, 25639519, 29251349, and 28114363 respectively).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
50% PBS, 50% Glycerol
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Purity
IgG purified

Alternate Names for Laminin Antibody

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • S-LAM alpha
  • S-laminin subunit alpha

Background

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kD. It plays an important role in many aspects of the cell biology. Laminin is composed of one A chain (400 kD) one B1 chain (215 kD) and one B2 chain (205 kD) all held together by disulfide bonds. The molecule has a cross-like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells. Laminins from various species have common antigenic determinants. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. It has been found to modulate cell differentiation, cell shaping and also cell movement because it appears to be an important cell substrate-adhesion protein. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. The greatest interest in laminin has been provoked by the discovery of its ability to promote neurite regeneration. Quantitation of laminin is beneficial by allowing for a method of non-invasive testing. For example, in fibrosis, a serious and frequent complication of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver, the concentration of laminin in serum is positively related to the severity of the fibrotic liver disease being significantly higher in cirrhosis than in mere fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB300-144
Species: Hu, Mu, Rt, ChHa, In, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl

Publications for Laminin Antibody (NB300-144)(61)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chinese Hamster, Invertebrate, Mammal, Rabbit, Sheep.

We have publications tested in 7 applications: Flow, ICC, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
Flow
(1)
ICC
(1)
ICC/IF
(18)
IHC
(14)
IHC-Fr
(6)
IHC-P
(5)
WB
(13)
All Applications
Filter By Species
Human
(13)
Mouse
(26)
Rat
(12)
Chinese Hamster
(1)
Invertebrate
(1)
Mammal
(1)
Rabbit
(1)
Sheep
(3)
All Species
Showing Publications 1 - 10 of 61. Show All 61 Publications.
Publications using NB300-144 Applications Species
Hanadhita D, Rahma A, Wahid MR et al. Extracellular matrix composition of different spleen compartments of fruit bats Anat Histol Embryol Dec 26 2019 [PMID: 31877588] (IHC, Mammal) IHC Mammal
Herrmann A, Lahtz C, Song J, et al. Integrin alpha 6 signaling induces STAT3-TET3-mediated hydroxymethylation of genes critical for maintenance of glioma stem cells Oncogene Dec 9 2019 [PMID: 31819166] (Flow, Human) Flow Human
Kunz L, Schroeder T A 3D Tissue-wide Digital Imaging Pipeline for Quantitation of Secreted Molecules Shows Absence of CXCL12 Gradients in Bone Marrow Cell Stem Cell Dec 5 2019 [PMID: 31809740] (ICC/IF, Mouse) ICC/IF Mouse
Wu D, Witt RL, Harrington DA, Farach-Carson MC Dynamic Assembly of Human Salivary Stem/Progenitor Microstructures Requires Coordinated alpha1 beta1 Integrin-Mediated Motility Front Cell Dev Biol Oct 16 2019 [PMID: 31750298] (ICC/IF, Human) ICC/IF Human
Ashok A, Kang MH, Wise AS et al. Prion protein modulates endothelial to mesenchyme-like transition in trabecular meshwork cells: Implications for primary open angle glaucoma Sci Rep Sep 11 2019 [PMID: 31511544] (IHC, WB, Human) IHC, WB Human
Choi S, Yu J, Park A et al. BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling Sci Rep Aug 13 2019 [PMID: 31409851] (WB, Human) WB Human
Miska J, Lee-Chang C, Rashidi A et al. HIF-1 alpha Is a Metabolic Switch between Glycolytic-Driven Migration and Oxidative Phosphorylation-Driven Immunosuppression of Tregs in Glioblastoma Cell Rep Apr 2 2019 [PMID: 30943404] (ICC/IF, Mouse) ICC/IF Mouse
Hamoud N, Tran V, Aimi T et al. Spatiotemporal regulation of the GPCR activity of BAI3 by C1qL4 and Stabilin-2 controls myoblast fusion. Nat Commun. Oct 26 2018 [PMID: 30367035] (IHC-Fr, Mouse) IHC-Fr Mouse
Mademtzoglou D, Asakura Y, Borok MJ et al. Cellular localization of the cell cycle inhibitor Cdkn1c controls growth arrest of adult skeletal muscle stem cells. Elife. Oct 4 2018 [PMID: 30284969] (IHC, Mouse) IHC Mouse
Kamynina A et al.. Furthering the understanding of the redox control of soluble Epoxide Hydrolase and Protein Kinase G in the cardiovascular system Thesis 2019 (IHC-Fr, Mouse)

Details:
Citation using the DyLight 488 format of this antibody.
IHC-Fr Mouse
Show All 61 Publications.

Reviews for Laminin Antibody (NB300-144) (7) 4.47

Average Rating: 4.4
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB300-144:
Filter by Applications
ICC
(3)
WB
(2)
IHC
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(4)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Laminin NB300-144
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reviewed by:
Anonymous
ICC Human 10/24/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedTM cells
SpeciesHuman
Lot196052516

Comments

Commentsuse at 1:100
Western Blot Laminin NB300-144
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reviewed by:
Anonymous
WB Human 10/24/2017
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Summary

ApplicationWestern Blot
Sample Testedhuman eye
SpeciesHuman
Lot196052516

Comments

Commentsuse at 1:500
Immunohistochemistry Laminin NB300-144
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reviewed by:
Anonymous
IHC Mouse 06/27/2017
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Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain
SpeciesMouse
Western Blot Laminin NB300-144
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reviewed by:
Anonymous
WB Mouse 06/15/2017
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Summary

ApplicationWestern Blot
Sample TestedMouse cornea
SpeciesMouse
Immunocytochemistry Laminin NB300-144
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reviewed by:
Anony- mous
ICC Human 06/04/2014
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Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of HeLa cells using Laminin antibody (NB300-144, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
FileView PDF
Immunocytochemistry Laminin NB300-144
Enlarge
reviewed by:
Gail Seigel
ICC Human 11/14/2012
View

Summary

ApplicationImmunocytochemistry
Sample TestedWERI-RB27 human retinoblastoma cells
SpeciesHuman
LotLot 1
CommentsThe antibody worked well on the first attempt

Blocking

Blocking DetailsAbcam protein block Ab64226, ready-to-use, 15 minutes at room temp.

Primary Anitbody

Dilution RatioLAMA1 1:200 dilution for one hour at room temp

Secondary Antibody

Secondary DescriptionTwo-step Polymer-HRP kit
Secondary Manufacturer Cat#Syd Laboratories PT03-D
Secondary Concentrationready-to-use

Details

Detection NotesDAB reagent for 5 minutes
Fixation DetailsCytospin preparations permeabilized with PBS-Tween for 5 minutes at room temp.
Wash DescriptionTPBS three times 5 minutes

Comments

CommentsThe antibody worked well on the first attempt
 
reviewed by:
Anonymous
IHC-Fr Rat 12/06/2011
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat
SpeciesRat

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Secondary Antibody

Secondary DescriptionSecondary Ab: Rabbit

Details

Detection NotesDetection Method: DAB

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Laminin Antibody (NB300-144). (Showing 1 - 2 of 2 FAQs).

  1. I have a question concerning the antibody NB300-144. The antibody blocks the function of the laminin. Can you tell me, which site of the laminin the antibody attacks? Because I need an antibody which block the binding from laminin to the integrin receptor by binding to the globular domain (integrin binding site) of the laminin.
    • Our bioassays show that this antibody binds to laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. In culture, the antibody hinders cell migration on a laminin substratum. It also inhibits neurite and cell process outgrowth. Applied to tissue sections, it inhibits neurite outgrowth by neurons seeded onto the tissue section. As this is a polyclonal antibody, it is nearly impossible to determine the specific antigen binding sites. No molecular level tests for laminin domain recognition have been performed. However, based on bioassays, it appears that the mix of antibodies in this purified polyclonal anti-serum bind multiply across the large laminin molecule to an extent that its biological activities are inhibited; that is, cell receptor binding is markedly hindered. It is the best, among many, laminin function-blocking preps we have tested. Most polyclonal antibodies raised in rabbit against a mouse laminin-1 will inhibit the activity of many rodent laminin isoforms. This one is especially specific, avid and purified for that purpose.
  2. Do you have any WB images tested by NB300-144 on human? I see the WB image on the datasheet, but I do not know what sample is loaded on each lane?
    • The samples used for the western blot image are Laminin-1 (LN-1) protein from EHS tumor and Laminin-2 (LN-2) protein from heart.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-144AF350
Alexa Fluor 405 NB300-144AF405
Alexa Fluor 488 NB300-144AF488
Alexa Fluor 532 NB300-144AF532
Alexa Fluor 594 NB300-144AF594
Alexa Fluor 647 NB300-144AF647
Alexa Fluor 700 NB300-144AF700
Alexa Fluor 750 NB300-144AF750
Allophycocyanin NB300-144APC
Allophycocyanin/Cy7 NB300-144APCCY7
Biotin NB300-144B
DyLight 350 NB300-144UV
DyLight 405 NB300-144V
DyLight 488 NB300-144G
DyLight 550 NB300-144R
DyLight 594 NB300-144DL594
DyLight 650 NB300-144C
DyLight 680 NB300-144FR
DyLight 755 NB300-144IR
FITC NB300-144F
HRP NB300-144H
Janelia Fluor 549 NB300-144JF549
Janelia Fluor 646 NB300-144JF646
PE NB300-144PE
PE/Atto594 NB300-144PEATT594
PE/Cy5.5 NB300-144PECY55
PE/Cy7 NB300-144PECY7
PerCP NB300-144PCP

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Blogs on Laminin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Recent Reviews

4.4
5
4
4
2
3
1
2
0
1
0

Anonymous
10/24/2017
Application: ICC
Species: Human

Anonymous
10/24/2017
Application: WB
Species: Human

Anonymous
06/27/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol LAMA1
Entrez
Uniprot
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