Laminin Antibody

Images

 
Western Blot: Laminin Antibody [NB300-144] - Analysis of rat heart cells lysates (lane 1) and 0.2 ug of purified laminin protein from mouse EHS sarcoma (lane 2) This antibody recognizes 3 laminin isotypes: alpha 1 ...read more
Immunocytochemistry/ Immunofluorescence: Laminin Antibody [NB300-144] - IF Confocal analysis of HeLa cells using Laminin antibody (NB300-144, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary ...read more
Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of mouse section of cortex stained with Laminin- (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of ...read more
Western Blot: Laminin Antibody [NB300-144] - Analysis of Laminin-1 expression in mouse EHS tumor crude extracts (left) and Laminin-2 expression in rat heart crude extracts (right). The Laminin polyclonal antibody was ...read more
Immunocytochemistry/ Immunofluorescence: Laminin Antibody [NB300-144] - Detection of Laminin (Green) in Hela cells using NB300-144 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using NB 300-144. Pepsin antigen retrieval was performed on this tissue sample.

Product Details

Summary
Reactivity Hu, Mu, Rt, In, Rb, ShSpecies Glossary
Applications WB, Func, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Laminin Antibody Summary

Immunogen
Laminin isolated from mouse EHS tumor. [UniProt# P19137]
Localization
Secreted, Basement membrane, Extracellular matrix
Marker
Basement Membrane Marker
Specificity
This is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LAMA1
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:5000
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Frozen 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
Application Notes
This Laminin antibody can be used for Immunocytochemistry/Immunofluorescence, Functional assays, Immunohistochemistry paraffin and frozen sections, and Western blotting where it detects bands at 200 and 400 kDa. The antibody functionally inhibits Laminin in mouse and rat. It binds to Laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue.
Reviewed Applications
Read 7 Reviews rated 4.4
using
NB300-144 in the following applications:

Publications
Read Publications using
NB300-144 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 18214989). Invertebrate reactivity reported in scientific literature (PMID: 28114363).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
IgG purified

Alternate Names for Laminin Antibody

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • S-LAM alpha
  • S-laminin subunit alpha

Background

Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with an approximate molecular weight of 900 kD. It plays an important role in many aspects of the cell biology. Laminin is composed of one A chain (400 kD) one B1 chain (215 kD) and one B2 chain (205 kD) all held together by disulfide bonds. The molecule has a cross-like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells. Laminins from various species have common antigenic determinants. Laminin is only found in significant quantities in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. It has been found to modulate cell differentiation, cell shaping and also cell movement because it appears to be an important cell substrate-adhesion protein. Variations in the expression of this protein have been observed in embryogenesis, organogenesis, post traumatic healing and cancer. The greatest interest in laminin has been provoked by the discovery of its ability to promote neurite regeneration. Quantitation of laminin is beneficial by allowing for a method of non-invasive testing. For example, in fibrosis, a serious and frequent complication of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver, the concentration of laminin in serum is positively related to the severity of the fibrotic liver disease being significantly higher in cirrhosis than in mere fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Laminin Antibody (NB300-144)(37)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Invertebrate, Rabbit, Sheep.

We have publications tested in 6 applications: ICC, ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC
(1)
ICC/IF
(11)
IHC
(7)
IHC-Fr
(4)
IHC-P
(3)
WB
(10)
All Applications
Filter By Species
Human
(5)
Mouse
(14)
Rat
(10)
Invertebrate
(1)
Rabbit
(1)
Sheep
(3)
All Species
Showing Publications 1 - 10 of 37. Show All 37 Publications.
Publications using NB300-144 Applications Species
Belair DG, Wolf CJ, Wood C et al. Engineering human cell spheroids to model embryonic tissue fusion in vitro PLoS ONE 2017 Sep 12 [PMID: 28898253] (ICC/IF, Human) ICC/IF Human
Gelmedin V, Morel M, Hahnel S et al. Evidence for Integrin - Venus Kinase Receptor 1 Alliance in the Ovary of Schistosoma mansoni Females Controlling Cell Survival. PLoS Pathog [PMID: 28114363] (ICC/IF, Invertebrate) ICC/IF Invertebrate
Shojaie S, Lee J, Wang J et al. Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds. J Vis Exp. May 5 2016 [PMID: 27214388]
Shojaie S, Lee J, Wang J et al. Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds. J Vis Exp. May 5 2016 [PMID: 27214388]
Fu M, Song Y, Wen Z et al. Inositol Hexaphosphate and Inositol Inhibit Colorectal Cancer Metastasis to the Liver in BALB/c Mice. Nutrients [PMID: 27187454]
Mori H, Yao Y, Learman BS et al. Induction of WNT11 by hypoxia and hypoxia-inducible factor-1alpha regulates cell proliferation, migration and invasion. Sci Rep. 2016 Feb 10 [PMID: 26861754] (WB, Human) WB Human
Patel PS, Kearney JF. Neonatal Exposure to Pneumococcal Phosphorylcholine Modulates the Development of House Dust Mite Allergy during Adult Life. J Immunol 2015 May 08 [PMID: 25957171] (IHC, Mouse)

Details:
Using the Biotin conjugated version of NB300-144, catalog number NB300-144B.
IHC Mouse
Wharton RE, Stefanov EK, King RG, Kearney JF. Antibodies Generated against Streptococci Protect in a Mouse Model of Disseminated Aspergillosis J. Immunol. 2015 May 01 [PMID: 25821219] (ICC/IF, Mouse) ICC/IF Mouse
Van der Donckt C, Roth L, Vanhoutte G et al. Fibrillin-1 impairment enhances blood-brain barrier permeability and xanthoma formation in brains of apolipoprotein E-deficient mice. Neuroscience. 2015 Mar 19 [PMID: 25797463] (WB, Mouse) WB Mouse
Pandya PR, Serra V, Green LM, Gridley DS. pKC Modulates Integrin Expression that Contributes to Fibrotic Changes in Irradiated Thyroid Tissue. In Vivo. 2015 Mar 20 [PMID: 25792644]
Show All 37 Publications.

Reviews for Laminin Antibody (NB300-144) (7) 4.47

Average Rating: 4.4
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Mouse, Rat.
We have 7 reviews tested in 4 application: ICC, WB, IHC, IHC-Fr.

Reviews using NB300-144:
Filter by Applications
ICC
(3)
WB
(2)
IHC
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Human
(4)
Mouse
(2)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Laminin NB300-144
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reviewed by:
Anonymous
ICC Human 10/24/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedTM cells
SpeciesHuman
Lot196052516

Comments

Commentsuse at 1:100
Western Blot Laminin NB300-144
Enlarge
reviewed by:
Anonymous
WB Human 10/24/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman eye
SpeciesHuman
Lot196052516

Comments

Commentsuse at 1:500
Immunohistochemistry Laminin NB300-144
Enlarge
reviewed by:
Anonymous
IHC Mouse 06/27/2017
View

Summary

ApplicationImmunohistochemistry
Sample Tested Mouse brain
SpeciesMouse
Western Blot Laminin NB300-144
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reviewed by:
Anonymous
WB Mouse 06/15/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse cornea
SpeciesMouse
Immunocytochemistry Laminin NB300-144
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reviewed by:
Anony- mous
ICC Human 06/04/2014
View

Summary

ApplicationImmunocytochemistry
SpeciesHuman
Special ApplicationsIF Confocal analysis of HeLa cells using Laminin antibody (NB300-144, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
FileView PDF
Immunocytochemistry Laminin NB300-144
Enlarge
reviewed by:
Gail Seigel
ICC Human 11/14/2012
View

Summary

ApplicationImmunocytochemistry
Sample TestedWERI-RB27 human retinoblastoma cells
SpeciesHuman
LotLot 1
CommentsThe antibody worked well on the first attempt

Blocking

Blocking DetailsAbcam protein block Ab64226, ready-to-use, 15 minutes at room temp.

Primary Anitbody

Dilution RatioLAMA1 1:200 dilution for one hour at room temp

Secondary Antibody

Secondary DescriptionTwo-step Polymer-HRP kit
Secondary Manufacturer Cat#Syd Laboratories PT03-D
Secondary Concentrationready-to-use

Details

Detection NotesDAB reagent for 5 minutes
Fixation DetailsCytospin preparations permeabilized with PBS-Tween for 5 minutes at room temp.
Wash DescriptionTPBS three times 5 minutes

Comments

CommentsThe antibody worked well on the first attempt
 
reviewed by:
Anonymous
IHC-Fr Rat 12/06/2011
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedRat
SpeciesRat

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Secondary Antibody

Secondary DescriptionSecondary Ab: Rabbit

Details

Detection NotesDetection Method: DAB

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Laminin Antibody (NB300-144). (Showing 1 - 2 of 2 FAQs).

  1. I have a question concerning the antibody NB300-144. The antibody blocks the function of the laminin. Can you tell me, which site of the laminin the antibody attacks? Because I need an antibody which block the binding from laminin to the integrin receptor by binding to the globular domain (integrin binding site) of the laminin.
    • Our bioassays show that this antibody binds to laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. In culture, the antibody hinders cell migration on a laminin substratum. It also inhibits neurite and cell process outgrowth. Applied to tissue sections, it inhibits neurite outgrowth by neurons seeded onto the tissue section. As this is a polyclonal antibody, it is nearly impossible to determine the specific antigen binding sites. No molecular level tests for laminin domain recognition have been performed. However, based on bioassays, it appears that the mix of antibodies in this purified polyclonal anti-serum bind multiply across the large laminin molecule to an extent that its biological activities are inhibited; that is, cell receptor binding is markedly hindered. It is the best, among many, laminin function-blocking preps we have tested. Most polyclonal antibodies raised in rabbit against a mouse laminin-1 will inhibit the activity of many rodent laminin isoforms. This one is especially specific, avid and purified for that purpose.
  2. Do you have any WB images tested by NB300-144 on human? I see the WB image on the datasheet, but I do not know what sample is loaded on each lane?
    • The samples used for the western blot image are Laminin-1 (LN-1) protein from EHS tumor and Laminin-2 (LN-2) protein from heart.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-144AF405
Alexa Fluor 488 Labeled NB300-144AF488
Alexa Fluor 647 Labeled NB300-144AF647
Alexa Fluor 700 Labeled NB300-144AF700
Biotin Labeled NB300-144B
DyLight 488 Labeled NB300-144G
DyLight 550 Labeled NB300-144R
DyLight 650 Labeled NB300-144C
HRP Labeled NB300-144H

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Blogs on Laminin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Recent Reviews

4.4
5
4
4
2
3
1
2
0
1
0

Anonymous
10/24/2017
Application: ICC
Species: Human

Anonymous
10/24/2017
Application: WB
Species: Human

Anonymous
06/27/2017
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol LAMA1
Entrez
Uniprot