Laminin Antibody


Western Blot: Laminin Antibody [NB300-144] - Analysis of rat heart cells lysates (lane 1) and 0.2 ug of purified laminin protein from mouse EHS sarcoma (lane 2) This antibody recognizes 3 laminin isotypes: alpha 1 more
Immunocytochemistry/ Immunofluorescence: Laminin Antibody [NB300-144] - IF Confocal analysis of HeLa cells using Laminin antibody (NB300-144, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary more
Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of mouse section of cortex stained with Laminin- (red). Blue is DAPI staining of DNA. This antibody reveals strong staining in the basement membranes of more
Western Blot: Laminin Antibody [NB300-144] - Analysis of Laminin-1 expression in mouse EHS tumor crude extracts (left) and Laminin-2 expression in rat heart crude extracts (right). The Laminin polyclonal antibody was more
Immunocytochemistry/ Immunofluorescence: Laminin Antibody [NB300-144] - Detection of Laminin (Green) in Hela cells using NB300-144 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Immunohistochemistry: Laminin Antibody [NB300-144] - Staining of rat spinal cord and dorsal root paraformaldehyde/paraffin-embedded tissue using NB 300-144. Pepsin antigen retrieval was performed on this tissue sample.

Product Details

Reactivity Hu, Mu, Rt, Rb, ShSpecies Glossary
Applications WB, Func, ICC/IF, IHC, IHC-Fr, IHC-P
1.0 mg/ml

Laminin Antibody Summary

Laminin isolated from mouse EHS tumor. [UniProt# P19137]
Secreted, Basement membrane, Extracellular matrix
Basement Membrane Marker
This is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
IgG purified


  • Western Blot 1:100-1:5000
  • Functional
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Frozen 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:500-1:2000
Application Notes
This Laminin antibody can be used for Immunocytochemistry/Immunofluorescence, Functional assays, Immunohistochemistry paraffin and frozen sections, and Western blotting where it detects bands at 200 and 400 kDa. The antibody functionally inhibits Laminin in mouse and rat. It binds to Laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue.
Reviewed Applications
Read 3 Reviews rated 4.7
NB300-144 in the following applications:

Read Publications using
NB300-144 in the following applications:

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 18214989).

Alternate Names for Laminin Antibody

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • S-LAM alpha
  • S-laminin subunit alpha


Laminin antibodies are widely used to label blood vessels and basement membranes.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC-P
Species: Hu, Mu
Applications: WB, Flow
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Rt
Applications: WB
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PLA
Species: Hu, Mu, Rt, Pm
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Ca, Dr, Eq, Ma, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, Simple Western, IHC, IHC-P
Species: Hu
Applications: WB, IHC
Species: Hu
Applications: WB, IHC
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC-P
Species: Hu, Mu, Rt, Rb, Sh
Applications: WB, Func, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Laminin Antibody (NB300-144)(33)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Rabbit, Sheep.

We have publications tested in 6 applications: ICC, ICC/IF, IHC, IHC-Fr, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB300-144 Applications Species
Shojaie S, Lee J, Wang J et al. Generation of ESC-derived Mouse Airway Epithelial Cells Using Decellularized Lung Scaffolds. J Vis Exp. May 24 2016 12:00AM [PMID:27214388]
Mori H, Yao Y, Learman BS et al. Induction of WNT11 by hypoxia and hypoxia-inducible factor-1alpha regulates cell proliferation, migration and invasion. Sci Rep. 2016 Feb 10 [PMID:26861754] (WB, Human) WB Human
Patel PS, Kearney JF. Neonatal Exposure to Pneumococcal Phosphorylcholine Modulates the Development of House Dust Mite Allergy during Adult Life. J Immunol 2015 May 08 [PMID:25957171] (IHC, Mouse) IHC Mouse
Wharton RE, Stefanov EK, King RG, Kearney JF. Antibodies Generated against Streptococci Protect in a Mouse Model of Disseminated Aspergillosis J. Immunol. 2015 May 01 [PMID:25821219] (ICC/IF, Mouse) ICC/IF Mouse
Van der Donckt C, Roth L, Vanhoutte G et al. Fibrillin-1 impairment enhances blood-brain barrier permeability and xanthoma formation in brains of apolipoprotein E-deficient mice. Neuroscience. 2015 Mar 19 [PMID:25797463] (WB, Mouse) WB Mouse
Pandya PR, Serra V, Green LM, Gridley DS. pKC Modulates Integrin Expression that Contributes to Fibrotic Changes in Irradiated Thyroid Tissue. In Vivo. 2015 Mar 20 [PMID:25792644]
Garvey SM, Russ DW, Skelding MB et al. Molecular and metabolomic effects of voluntary running wheel activity on skeletal muscle in late middle-aged rats Physiol Rep. 2015 Feb 25 [PMID:25716928] (IHC, Rat) IHC Rat
Castillo-Melendez M, Yawno T, Allison BJ et al. Cerebrovascular adaptations to chronic hypoxia in the growth restricted lamb Int. J. Dev. Neurosci. 2015 Jan 29 [PMID:25639519] (IHC-P, Sheep) IHC-P Sheep
Alhusban A, Fouda Ay, Bindu Pillai et al. Compound 21 is pro-angiogenic in the brain and results in sustained recovery after ischemic stroke. J. Hypertens. 2014 Oct 08 [PMID:25304472] (IHC, Rat) IHC Rat
Mancuso L, Gualerzi A, Boschetti F et al. Decellularized ovine arteries as small-diameter vascular grafts. Biomed Mater 2014 Aug 01 [PMID:25050540] (IHC-P, Sheep) IHC-P Sheep
Show All 33 Publications.

Reviews for Laminin Antibody (NB300-144) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Rat.
We have 3 reviews tested in 2 application: ICC, IHC-Fr.

Reviews using NB300-144:
Filter by Applications
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Laminin NB300-144
reviewed by:
ICC Human 06/04/2014


Special ApplicationsIF Confocal analysis of HeLa cells using Laminin antibody (NB300-144, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green, A). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red, B). DAPI was used to stain the cell nuclei (blue, C).
FileView PDF
Immunocytochemistry Laminin NB300-144
reviewed by:
ICC Human 11/14/2012


Sample TestedWERI-RB27 human retinoblastoma cells
LotLot 1
CommentsThe antibody worked well on the first attempt


Blocking DetailsAbcam protein block Ab64226, ready-to-use, 15 minutes at room temp.

Primary Anitbody

Dilution RatioLAMA1 1:200 dilution for one hour at room temp

Secondary Antibody

Secondary DescriptionTwo-step Polymer-HRP kit
Secondary Manufacturer Cat#Syd Laboratories PT03-D
Secondary Concentrationready-to-use


Detection NotesDAB reagent for 5 minutes
Fixation DetailsCytospin preparations permeabilized with PBS-Tween for 5 minutes at room temp.
Wash DescriptionTPBS three times 5 minutes


CommentsThe antibody worked well on the first attempt
reviewed by:
IHC-Fr Rat 12/06/2011


Sample TestedRat

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000

Secondary Antibody

Secondary DescriptionSecondary Ab: Rabbit


Detection NotesDetection Method: DAB

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Laminin Antibody (NB300-144). (Showing 1 - 2 of 2 FAQs).

  1. I have a question concerning the antibody NB300-144. The antibody blocks the function of the laminin. Can you tell me, which site of the laminin the antibody attacks? Because I need an antibody which block the binding from laminin to the integrin receptor by binding to the globular domain (integrin binding site) of the laminin.
    • Our bioassays show that this antibody binds to laminin and inhibits most, if not all, of its cell adhesion and growth promotive properties. In culture, the antibody hinders cell migration on a laminin substratum. It also inhibits neurite and cell process outgrowth. Applied to tissue sections, it inhibits neurite outgrowth by neurons seeded onto the tissue section. As this is a polyclonal antibody, it is nearly impossible to determine the specific antigen binding sites. No molecular level tests for laminin domain recognition have been performed. However, based on bioassays, it appears that the mix of antibodies in this purified polyclonal anti-serum bind multiply across the large laminin molecule to an extent that its biological activities are inhibited; that is, cell receptor binding is markedly hindered. It is the best, among many, laminin function-blocking preps we have tested. Most polyclonal antibodies raised in rabbit against a mouse laminin-1 will inhibit the activity of many rodent laminin isoforms. This one is especially specific, avid and purified for that purpose.
  2. Do you have any WB images tested by NB300-144 on human? I see the WB image on the datasheet, but I do not know what sample is loaded on each lane?
    • The samples used for the western blot image are Laminin-1 (LN-1) protein from EHS tumor and Laminin-2 (LN-2) protein from heart.

Secondary Antibodies


Isotype Controls

Other Available Formats

Additional Laminin Antibody Products

Related Products by Gene

Bioinformatics Tool for Laminin Antibody (NB300-144)

Discover related pathways, diseases and genes to Laminin Antibody (NB300-144). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Laminin Antibody (NB300-144)

Discover more about diseases related to Laminin Antibody (NB300-144).

Pathways for Laminin Antibody (NB300-144)

View related products by pathway.

PTMs for Laminin Antibody (NB300-144)

Learn more about PTMs related to Laminin Antibody (NB300-144).

Research Areas for Laminin Antibody (NB300-144)

Find related products by research area.

Blogs on Laminin.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol LAMA1

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB300-144 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought