PSP/BPIFA2 Antibody (1F12) - BSA Free

Western Blot: PSP/BPIFA2 Antibody (1F12) [NBP2-37493] - Western blot analysis using mouse Splunc2 mAb against mouse Splunc2 (AA: 16-169) recombinant protein. (Expected MW is 41.6 kDa)

Immunocytochemistry/Immunofluorescence: PSP/BPIFA2 Antibody (1F12) [NBP2-37493] - Immunofluorescence analysis of HepG2 cells using mouse Splunc2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: PSP/BPIFA2 Antibody (1F12) [NBP2-37493] - Immunohistochemical analysis of paraffin-embedded prostate tissues using mouse Splunc2 mouse mAb with DAB staining.

Flow Cytometry: PSP/BPIFA2 Antibody (1F12) [NBP2-37493] - Flow cytometric analysis of HeLa cells using mouse Splunc2 mouse mAb (green) and negative control (red).

Immunocytochemistry/Immunofluorescence: PSP/BPIFA2 Antibody (1F12) [NBP2-37493] - Immunofluorescence analysis of HepG2 cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: PSP/BPIFA2 Antibody (1F12) [NBP2-37493] - Immunohistochemical analysis of paraffin-embedded kidney tissues using mouse Splunc2 mouse mAb with DAB staining.

ELISA: PSP/BPIFA2 Antibody (1F12) [NBP2-37493] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu, Po
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
• Clone: 1F12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

PSP/BPIFA2 Antibody (1F12) - BSA Free Summary

• Immunogen: Purified recombinant fragment of mouse Splunc2 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BPIFA2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 31268775).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for PSP/BPIFA2 Antibody (1F12) - BSA Free

• bA49G10.1
• BPIFA2
• C20orf70
• chromosome 20 open reading frame 70
• Parotid secretory protein
• PSP
• short palate, lung and nasal epithelium carcinoma associated 2
• short palate, lung and nasal epithelium carcinoma-associated protein 2
• SPLUNC2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PSP/BPIFA2 Antibody (NBP2-37493)(1)

Publication using NBP2-37493

• Sallustio F, Stasi A, Curci C et al. Renal progenitor cells revert LPS-induced endothelial-to-mesenchymal transition by secreting CXCL6, SAA4, and BPIFA2 antiseptic peptides FASEB J. 2019-07-03 [PMID: 31268775] (IF/IHC, Porcine)

Bioinformatics

• Gene Symbol: BPIFA2