c-Abl Antibody (8E9)

Western Blot: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of c-Abl using c-Abl Monoclonal Antibody on HeLa Cells.

Immunocytochemistry/Immunofluorescence: c-Abl Antibody (8E9) [NBP2-49695] - taining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a c-Abl monoclonal antibody in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Immunohistochemistry-Paraffin: c-Abl Antibody (8E9) [NBP2-49695] - Analysis of human placenta stained with cbl antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of trophoblasts.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP
• Clone: 8E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

c-Abl Antibody (8E9) Summary

• Description: Novus Biologicals Mouse c-Abl Antibody (8E9) (NBP2-49695) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant Abl protein
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABL1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Immunoprecipitation 2 ug/mg
  • Western Blot 1 ug/mL

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4) and 0.2% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for c-Abl Antibody (8E9)

• Abelson murine leukemia viral oncogene homolog 1
• ABL
• ABL1
• bcr/abl
• BCR-ABL1
• c-abl oncogene 1, non-receptor tyrosine kinase
• c-abl oncogene 1, receptor tyrosine kinase
• cAbl
• c-Abl
• EC 2.7.10
• EC 2.7.10.2
• JTK7
• JTK7bcr/abl
• p150bcr/c-abl oncogene protein
• Proto-oncogene c-Abl
• proto-oncogene tyrosine-protein kinase ABL1
• tyrosine-protein kinase ABL1
• v-abl Abelson murine leukemia viral oncogene homolog 1
• v-abl

Background

c-Abl is the human cellular homolog of a the v-Abl viral oncogene. v-Abl was originally discovered as a mouse cell-derived sequence in the genome of the Abelson murine leukemia virus (A-MuLV), a transforming retrovirus isolated by the laboratory of Dr. H.T. Abelson. c-Abl is a tyrosine kinase from the Src-family of tyrosine kinases that localizes to both the cytoplasm and nucleus. It bears an SH2 (src-homology 2) and SH3 (src-homology 3) domain responsible for mediating c-Abl protein-protein interactions. c-Abl is implicated to play a role in multiple cellular processes such as cell cycle checkpoint signaling, apoptosis, cell differentiation, cell adhesion, and transcriptional regulation. Chromosomal translocations involving the c-Abl gene are associated with human leukemias.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for c-Abl Antibody (NBP2-49695) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pauline Jeannot ICC Human 11/16/2023

Summary

• Application: Immunocytochemistry
• Sample Tested: Human primary astrocytes
• Species: Human
• Lot: TI2611057

Bioinformatics

• Gene Symbol: ABL1