Laminin beta 1 Antibody (2D9G5)

Western Blot: Laminin beta 1 Antibody (2D9G5) [NBP1-51558] - Analysis using LAMB1 mouse mAb against truncated LAMB1-His recombinant protein (1).

Immunocytochemistry/Immunofluorescence: Laminin beta 1 Antibody (2D9G5) [NBP1-51558] - Human salivary cells express laminin beta 1 (red) and direct its secretion to the cell membrane in 3D hyaluronan gels. Image from verified customer review.

Immunohistochemistry-Paraffin: Laminin beta 1 Antibody (2D9G5) [NBP1-51558] - Analysis of paraffin-embedded human Thyroid tissues using LAMB1 mouse mAb

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 2D9G5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Laminin beta 1 Antibody (2D9G5) Summary

• Description: Novus Biologicals Mouse Laminin beta 1 Antibody (2D9G5) (NBP1-51558) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of Laminin beta 1 (aa31-270) expressed in E. Coli.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: LAMB1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:2000
• Application Notes: ICC/IF reported in verified customer review.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Laminin beta 1 Antibody (2D9G5)

• CLM
• cutis laxa with marfanoid phenotype
• Laminin B1 chain
• laminin subunit beta-1
• laminin, beta 1
• Laminin-1 subunit beta
• Laminin-10 subunit beta
• Laminin-12 subunit beta
• Laminin-2 subunit beta
• Laminin-6 subunit beta
• Laminin-8 subunit beta
• MGC142015

Background

Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of Laminins with stabilizing type IV collagen networks, nidogen and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels and surrounding muscle, peripheral nerve and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression and tissue architecture. Each Laminin is a heterotrimer of alpha, beta and gamma chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble and bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan and cognate Laminin receptors. The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Laminin beta 1 Antibody (NBP1-51558) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 12/10/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Primary human salivary cells
• Species: Human
• Lot: 150609

Comments

• Comments: Used at a dilution of 1:100
  Blocked with goat serum

Bioinformatics

• Gene Symbol: LAMB1