Laminin gamma 1 Antibody (A5)

Images

 
Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Laminin gamma 1 Antibody (A5).
Immunohistochemistry-Frozen: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Bovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then ...read more
Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Human Renal Cell Carcinoma stained with Laminin Monoclonal Antibody (A5).

Product Details

Summary
Reactivity Hu, Mu, BvSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ICC/IF
Clone
A5
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Laminin gamma 1 Antibody (A5) Summary

Immunogen
Murine EHS laminin preparation
Localization
Basement membrane
Specificity
Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This MAb reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
LAMC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
Application Notes
Hu-chromosome location: 1q31
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. IHC-Fr reported by verified by customer review. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:31830002).
Theoretical MW
210 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-44751 in the following applications:

Publications
Read Publications using
NBP2-44751 in the following applications:

Reactivity Notes

Bovine reported by a verified customer review.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Laminin gamma 1 Antibody (A5)

  • LAMB2Laminin-7 subunit gamma
  • LAMC1
  • Laminin B2 chain
  • Laminin B2
  • Laminin gamma 1
  • laminin subunit gamma-1
  • laminin, gamma 1 (formerly LAMB2)
  • Laminin-1 subunit gamma
  • Laminin-10 subunit gamma
  • Laminin-11 subunit gamma
  • Laminin-2 subunit gamma
  • Laminin-3 subunit gamma
  • Laminin-4 subunit gamma
  • Laminin-6 subunit gamma
  • Laminin-8 subunit gamma
  • Laminin-9 subunit gamma
  • MGC87297
  • S-LAM gamma
  • S-laminin subunit gamma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Laminin gamma 1 Antibody (NBP2-44751)(3)

Review for Laminin gamma 1 Antibody (NBP2-44751) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Bovine.

Reviews using NBP2-44751:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
Bovine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Laminin gamma 1 NBP2-44751
Enlarge
5
reviewed by:
Xing Fu
IHC-Fr Bovine 06/01/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSkeletal muscle
SpeciesBovine
Lot3915-1P171005

Comments

CommentsBovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then stained with a goat anti-rat-Alexa Fluor 488 secondary antibody (green) for 1 hour at room temperature. Section was then mounted in a mounting medium containing Dapi (blue).

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Laminin gamma 1 Antibody (NBP2-44751) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47842AF350
Alexa Fluor 405 NBP2-47842AF405
Alexa Fluor 488 NBP2-47842AF488
Alexa Fluor 532 NBP2-47842AF532
Alexa Fluor 594 NBP2-47842AF594
Alexa Fluor 647 NBP2-47842AF647
Alexa Fluor 700 NBP2-47842AF700
Alexa Fluor 750 NBP2-47842AF750
Allophycocyanin NBP2-47842APC
Biotin NBP2-47842B
DyLight 350 NBP2-47842UV
DyLight 405 NBP2-47842V
DyLight 488 NBP2-47842G
DyLight 550 NBP2-47842R
DyLight 594 NBP2-47842DL594
DyLight 650 NBP2-47842C
DyLight 680 NBP2-47842FR
DyLight 755 NBP2-47842IR
FITC NBP2-47842F
HRP NBP2-47842H
Janelia Fluor 549 NBP2-47842JF549
Janelia Fluor 646 NBP2-47842JF646
PE NBP2-47842PE
PerCP NBP2-47842PCP

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Xing Fu
06/01/2018
Application: IHC-Fr
Species: Bovine

Bioinformatics

Gene Symbol LAMC1
Uniprot