Immunohistochemistry-Frozen: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Bovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then ...read more
Immunohistochemistry-Paraffin: Laminin gamma 1 Antibody (A5) [NBP2-44751] - Human Renal Cell Carcinoma stained with Laminin Monoclonal Antibody (A5).
Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This MAb reacts with laminin B2/1 chain of ~210kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.
Protein A or G purified
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Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
210 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP2-44751 in the following applications:
Bovine frozen skeletal muscle section was fixed in 4% PFA for 5 min and stained with anti-laminin antibody (NBP2-44751) overnight at 4 C and then stained with a goat anti-rat-Alexa Fluor 488 secondary antibody (green) for 1 hour at room temperature. Section was then mounted in a mounting medium containing Dapi (blue).
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