Laminin Antibody [Alexa Fluor® 647]


Immunohistochemistry: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - SOX17 regulates adult muscle regeneration after injury in Pax7CreERT2/+;Sox17fl/fl mutant mice. Representative images of cryosections more
Flow Cytometry: Laminin Antibody [Alexa Fluor® 647] [NB300-144AF647] - A surface stain was performed on HeLa cells with Laminin Antibody NB300-144AF647 (blue) and a matched isotype control (orange). Cells were more

Product Details

Reactivity Hu, Mu, Rt, ChHa, In, Ma, Rb, ShSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl
Alexa Fluor 647

Order Details

Laminin Antibody [Alexa Fluor® 647] Summary

Laminin Antibody was made to Laminin 111 isolated from mouse Engelbreth-Holm-Swarm (EHS) sarcoma cells. [UniProt# P19137]
Secreted, Basement membrane, Extracellular matrix
Basement Membrane Marker
Laminin Antibody is pan-specific and reacts well with all Laminin isoforms tested: Laminin-1 (alpha-1, beta-1, and gamma-1) and Laminin-2 (alpha-2, beta-1, and gamma-1).
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunohistochemistry Free-Floating
Application Notes
This Laminin antibody can be used for Immunocytochemistry/Immunofluorescence, Functional assays, Immunohistochemistry paraffin and frozen sections, and Western blotting where it detects bands at 200 and 400 kDa. Immunostaining is enhanced by antigen retrieval with pepsin, especially paraffin tissue.
Read Publications using
NB300-144AF647 in the following applications:

Reactivity Notes

Rabbit, Fruit Bat, Chinese Hamster, and S. mansoni reactivity reported in scientific literature (PMID: 18214989, 31877588, 29251349, and 28114363 respectively). Human, Mouse, Rat, and Sheep reported in multiple pieces of scientific literature.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
IgG purified


Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Laminin Antibody [Alexa Fluor® 647]

  • LAMA
  • Laminin A chain
  • laminin subunit alpha-1
  • laminin, alpha 1
  • Laminin-1 subunit alpha
  • Laminin-3 subunit alpha
  • S-LAM alpha
  • S-laminin subunit alpha


Laminin, the most abundant structural and biologically active component in basement membranes, is a complex extracellular glycoprotein with high molecular weight (900 kDa). Laminin is composed of a laminin alpha (400 kDa), beta (215 kDa) and gamma chain (205 kDa), whose multidomain proteins are encoded by distinct genes (LAMA, LAMB, LAMC respectively) and have several isoforms of each chain. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, reflecting diverse functions in vivo. Laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Laminins are an important and biologically active part of the basal lamina, influencing cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis, where anti-laminin antibodies can be widely used to label blood vessels and basement membranes (1). Significant quantities of laminin are found in basement membranes, the thin extracellular matrices that surround epithelial tissue, nerve, fat cells and smooth, striated and cardiac muscle. Excessive serum laminin levels have been associated with fibrosis, cirrhosis and hepatitis, serious and frequent complications of chronic active liver disease characterized by excessive deposition of various normal components of connective tissue in liver (2). Epithelial mesenchymal transition (EMT) biomarkers include fibronectin, laminin, N-cadherin, and Slug (3).


1. Yang, M. Y., Chiao, M. T., Lee, H. T., Chen, C. M., Yang, Y. C., Shen, C. C., & Ma, H. I. (2015). An innovative three-dimensional gelatin foam culture system for improved study of glioblastoma stem cell behavior. J Biomed Mater Res B Appl Biomater, 103(3), 618-628. doi:10.1002/jbm.b.33214

2. Mak, K. M., & Mei, R. (2017). Basement Membrane Type IV Collagen and Laminin: An Overview of Their Biology and Value as Fibrosis Biomarkers of Liver Disease. Anat Rec (Hoboken), 300(8), 1371-1390. doi:10.1002/ar.23567

3. Choi, S., Yu, J., Park, A., Dubon, M. J., Do, J., Kim, Y.,... Park, K. S. (2019). BMP-4 enhances epithelial mesenchymal transition and cancer stem cell properties of breast cancer cells via Notch signaling. Sci Rep, 9(1), 11724. doi:10.1038/s41598-019-48190-5


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu
Applications: WB, ICC/IF, IP
Species: Hu, Pm, Pm, Bv(-), Ca(-), Po(-), Rt(-)
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IF
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu
Applications: WB, Simple Western
Species: Hu
Applications: WB
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC, KO
Species: Hu, Mu, Dr
Applications: WB
Species: Hu, Mu, Rt, Dr, Rb
Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, Flow-CS, Flow-IC
Species: Hu, Mu, Bv
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IF
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu, Po
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
Species: Hu, Mu, Rt, ChHa, In, Ma, Rb, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-FrFl

Publications for Laminin Antibody (NB300-144AF647)(4)

Reviews for Laminin Antibody (NB300-144AF647) (0)

There are no reviews for Laminin Antibody (NB300-144AF647). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Laminin Antibody (NB300-144AF647). (Showing 1 - 4 of 4 FAQs).

  1. What research areas can this product be used in?
    • All Laminin products can be used in: Angiogenesis, Apoptosis, Cancer, Cell Biology, Cellular Markers, Extracellular Matrix, Neuroscience, Signal Transduction, Tumor Suppressors.
  2. What is the theoretical molecular weight for your Laminin antibodies?
    • The theoretical molecular weight for Laminin antibodies is 337 kDa.
  3. If this product is used in an application or species as a part of a customer review, will that validate this product in the application/species?
    • If any of our primary antibodes are used in an untested application or species and it is shown to work through images from customer reviews or through publications, this validates the application/species for this product. Please check out our Innovator's Reward Program if you decide to test an antibody with a species or application that is not currently listed.
  4. I'm looking for a basement membrane marker, can you suggest a Laminin antibody that can be used for this purpose?
    • The following Laminin antibodies are Basement Membrane markers: NB120-17107, NB300-144, NB600-883, and NBP1-51759.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB300-144AF350
Alexa Fluor 405 NB300-144AF405
Alexa Fluor 488 NB300-144AF488
Alexa Fluor 532 NB300-144AF532
Alexa Fluor 594 NB300-144AF594
Alexa Fluor 647 NB300-144AF647
Alexa Fluor 700 NB300-144AF700
Alexa Fluor 750 NB300-144AF750
Allophycocyanin NB300-144APC
Allophycocyanin/Cy7 NB300-144APCCY7
Biotin NB300-144B
DyLight 350 NB300-144UV
DyLight 405 NB300-144V
DyLight 488 NB300-144G
DyLight 550 NB300-144R
DyLight 594 NB300-144DL594
DyLight 650 NB300-144C
DyLight 680 NB300-144FR
DyLight 755 NB300-144IR
FITC NB300-144F
HRP NB300-144H
Janelia Fluor 549 NB300-144JF549
Janelia Fluor 646 NB300-144JF646
PE NB300-144PE
PE/Atto594 NB300-144PEATT594
PE/Cy5.5 NB300-144PECY55
PE/Cy7 NB300-144PECY7
PerCP NB300-144PCP

Additional Laminin Products

Bioinformatics Tool for Laminin Antibody (NB300-144AF647)

Discover related pathways, diseases and genes to Laminin Antibody (NB300-144AF647). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Laminin Antibody (NB300-144AF647)

Discover more about diseases related to Laminin Antibody (NB300-144AF647).

Pathways for Laminin Antibody (NB300-144AF647)

View related products by pathway.

PTMs for Laminin Antibody (NB300-144AF647)

Learn more about PTMs related to Laminin Antibody (NB300-144AF647).

Research Areas for Laminin Antibody (NB300-144AF647)

Find related products by research area.

Blogs on Laminin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Could Laminin be Used to Treat Duchenne Muscular Dystrophy?
Duchenne muscular dystrophy (DMD) is a severe muscle wasting condition, causing disability and early death. There is currently no cure or adequate treatment for DMD, but pioneering research indicates that injection of a laminin protein may prevent (or...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Laminin Antibody [Alexa Fluor® 647] and receive a gift card or discount.


Gene Symbol LAMA1