SPANXB1 Antibody

Images

 
Western Blot: SPANXB1 Antibody [H00728695-B01P] - Analysis of SPANXB1 expression in transfected 293T cell line by SPANXB1 polyclonal antibody. Lane 1: SPANXB1 transfected lysate(11.33 KDa). Lane 2: Non-transfected ...read more
Immunocytochemistry/ Immunofluorescence: SPANXB1 Antibody [H00728695-B01P] - Analysis of purified antibody to SPANXB1 on Hs 181.Tes cell. (antibody concentration 10 ug/ml)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SPANXB1 Antibody Summary

Immunogen
SPANXB1 (NP_115850.1, 1 a.a. ~ 103 a.a) full-length human protein.MGQQSSVRRLKRSVPCESNEANEANEANKTMPETPTGDSDPQPAPKKMKTSESSTILVVRYRRNVKRTSPEELVNDHARENRINPDQMEEEEFIEITTERPKK
Specificity
Reacts with SPANX family, member B1.
Isotype
IgG
Clonality
Polyclonal
Host
Mouse
Gene
SPANXB1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This antibody is reactive against transfected lysate in western blot, immunofluorescence, and as a detection antibody in ELISA.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SPANXB1 Antibody

  • B1
  • C
  • Cancer/testis antigen 11.2
  • cancer/testis antigen family 11, member 2
  • CT11.1
  • CT11.2
  • CT11.2B1
  • CT11.3
  • CT11.4
  • CTp11
  • dJ171K16.1
  • NAP-X
  • Nuclear-associated protein SPAN-Xb
  • Nuclear-associated protein SPAN-Xf
  • SPANX family member B/F
  • SPANX family, member B1
  • SPANX
  • SPANXA
  • SPAN-Xa
  • SPANX-A
  • SPANXB
  • SPAN-Xb
  • SPANX-B
  • SPANXB2
  • SPANX-C
  • SPANX-D
  • SPANXE SPANX family, member E
  • SPAN-Xf
  • SPANX-F
  • SPANXF1
  • sperm protein associated with the nucleus on the X chromosome B/F
  • sperm protein associated with the nucleus, X chromosome, family member B1

Background

Temporally regulated transcription and translation of several testis-specific genes is required to initiate the series of molecular and morphological changes in the male germ cell lineage necessary for the formation of mature spermatozoa. This gene is a member of the SPANX family of cancer/testis-associated genes, which are located in a cluster on chromosome X. The SPANX genes encode differentially expressed testis-specific proteins that localize to various subcellular compartments. This particular gene maps to chromosome X in a head-to-tail orientation with SPANX family member B2, which appears to be a duplication of the B1 locus. The SPANXB genes are unique members of this gene family, since they contain an additional 18 nt in their coding region compared to the majority of family members. Although the protein encoded by this gene contains consensus nuclear localization signals, the major site for subcellular localization of expressed protein is in the cytoplasmic droplets of ejaculated spermatozoa. This protein provides a biochemical marker for studying the unique structures in spermatazoa, while attempting to further define its role in spermatogenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SPANXB1
Entrez
Uniprot