MEK1 Antibody (OTI1F5)

Images

 
Western Blot: MEK1 Antibody (1F5) [NBP1-47833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: MEK1 Antibody (1F5) [NBP1-47833] Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK1.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded prostate tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded colon tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded endometrium tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Ovary tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded pancreas tissue using anti-MEK1mouse monoclonal antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
OTI1F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.99 mg/ml

MEK1 Antibody (OTI1F5) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human MAP2K1 expression vector
Specificity
This antibody is specific for Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP2K1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
Theoretical MW
43.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
MEK1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47833 in the following applications:

Publications
Read Publication using
NBP1-47833 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.99 mg/ml
Purity
Affinity purified

Alternate Names for MEK1 Antibody (OTI1F5)

  • dual specificity mitogen-activated protein kinase kinase 1
  • ERK activator kinase 1
  • MAP kinase kinase 1
  • MAP2K1
  • MAPK/ERK kinase 1
  • MAPKK1
  • MEK1
  • MEK1MAPKK 1
  • mitogen-activated protein kinase kinase 1
  • MKK1
  • MKK1MEK 1
  • PRKMK1EC 2.7.12.2
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MEK1 Antibody (NBP1-47833)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for MEK1 Antibody (NBP1-47833) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-47833:
Filter by Applications
WB
(1)
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Filter by Species
Other
(1)
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Images Ratings Applications Species Date Details
Western Blot MEK1 NBP1-47833
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reviewed by:
Anonymous
WB Other 10/04/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesOther
LotN/A

Blocking

Blocking DetailsBlocking with 5% non-fat milk for 30 min

Primary Anitbody

Dilution Ratio1:2000, 2 hours

Secondary Antibody

Secondary DescriptionSheep anti-mouse IgG, HRP
Secondary Manufacturer Cat#GE Healthcare, NA931V
Secondary Concentration1:3000

Details

Detection NotesRegular Western blot Protocol

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MEK1 Antibody (NBP1-47833) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-72643AF350
Alexa Fluor 405 NBP2-72643AF405
Alexa Fluor 488 NBP2-72643AF488
Alexa Fluor 532 NBP2-72643AF532
Alexa Fluor 594 NBP2-72643AF594
Alexa Fluor 647 NBP2-72643AF647
Alexa Fluor 700 NBP2-72643AF700
Alexa Fluor 750 NBP2-72643AF750
Biotin NBP2-72643B
DyLight 350 NBP2-72643UV
DyLight 405 NBP2-72643V
DyLight 488 NBP2-72643G
DyLight 550 NBP2-72643R
DyLight 594 NBP2-72643DL594
DyLight 650 NBP2-72643C
DyLight 680 NBP2-72643FR
DyLight 755 NBP2-72643IR
FITC NBP2-72643F
HRP NBP2-72643H
Janelia Fluor 549 NBP2-72643JF549
Janelia Fluor 646 NBP2-72643JF646

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Contact Information

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/04/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol MAP2K1
Entrez
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