MEK1 Antibody (OTI1F5)

Images

 
Western Blot: MEK1 Antibody (1F5) [NBP1-47833] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEK1(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ...read more
Immunocytochemistry/ Immunofluorescence: MEK1 Antibody (1F5) [NBP1-47833] Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK1.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded prostate tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded colon tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded endometrium tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded Ovary tissue using anti-MEK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MEK1 Antibody (1F5) [NBP1-47833] - Staining of paraffin-embedded pancreas tissue using anti-MEK1mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.99 mg/ml

Order Details

MEK1 Antibody (OTI1F5) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human MAP2K1 expression vector
Specificity
This antibody is specific for Homo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MAP2K1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:2000
Theoretical MW
43.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-47833 in the following applications:

Publications
Read Publication using
NBP1-47833 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.99 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MEK1 Antibody (OTI1F5)

  • dual specificity mitogen-activated protein kinase kinase 1
  • ERK activator kinase 1
  • MAP kinase kinase 1
  • MAP2K1
  • MAPK/ERK kinase 1
  • MAPKK1
  • MEK1
  • MEK1MAPKK 1
  • mitogen-activated protein kinase kinase 1
  • MKK1
  • MKK1MEK 1
  • PRKMK1EC 2.7.12.2
  • protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

Background

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEK1 Antibody (NBP1-47833)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Review for MEK1 Antibody (NBP1-47833) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-47833:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Western Blot MEK1 NBP1-47833
Enlarge
5
reviewed by:
Verified Customer
WB Other 10/04/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman cancer cell whole cell lysate
SpeciesOther
LotN/A

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MEK1 Antibody (NBP1-47833) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.
mFluor Violet Conjugated Antibodies

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Recent Reviews

5
5
1
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1
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Verified Customer
10/04/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol MAP2K1
Entrez