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Fibronectin Antibody - BSA Free

Images

 
Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans ...read more
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The ...read more
Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.
Biological Strategies: Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Nicotinamide (NAM) attenuates unilateral urethral obstruction (UUO)-induced renal interstitial fibrosis. C57BL/6 mice were ...read more
Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.
Western Blot: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate.
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - Fibronectin antibody was tested in HeLa cells with DyLight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI ...read more
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody - BSA Free [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. ...read more
Immunohistochemistry: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in mouse kidney tissue section using anti-Fibronectin antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Fibronectin Antibody - BSA Free [NBP1-91258] - Analysis of Fibronectin in human oesophageal cancer tissue using anti-Fibronectin antibody. Image from verified customer review.
Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent ...read more
Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody ...read more
Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Mouse brain cryosections were stained with Fibronectin and anti-rabbit Alexa Fluor 555. Magnification 20x. Image from vrified customer review.
Immunohistochemistry-Frozen: Fibronectin Antibody - BSA Free [NBP1-91258] - Staining in mouse carotid artery. Image from a verified customer review.
Simple Western: Fibronectin Antibody - BSA Free [NBP1-91258] - Lane view shows a specific band for Fibronectin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Nicotinamide (NAM) attenuates unilateral urethral obstruction (UUO)-induced renal interstitial fibrosis. C57BL/6 mice were subjected to UUO surgery or sham operation. Different doses of NAM or saline were ...read more
LC depressed both the protein and mRNA level of fibronectin via depletion of peritoneal M2. Values were expressed as the mean +/- SD. (D) Immunofluorescence staining of fibronectin in the four groups. Blue corresponds ...read more
Lamin A/C-53BP1 interaction is regulated in a DNA damage-dependent manner. (A) Left; HDF were subjected to IR (10 Gy) and allowed to recover for 1 h. Association between A-type lamins and 53BP1 was assessed by ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Eq, FeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

Fibronectin Antibody - BSA Free Summary

Immunogen
A synthetic peptide made toward the C-terminal region of the human Fibronectin protein (canonical isoform within residues 2300-2380). [Swiss-Prot: P02751]
Localization
Secreted, extracellular space, extracellular matrix.
Marker
Mesenchymal Cells Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:400
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen reported by customer review
  • Immunohistochemistry-Paraffin 1:400
  • Simple Western 1:100
  • Western Blot 1:1000
Application Notes
In Western Blot, a band is seen at ~262 kDa representing Fibronectin. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed in the cytoplasm and extracellular space of mouse prostate tissue. Antigen retrieval with 10mM sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The 12-230kDa separation system and EZ Standard Pack 5 are recommended for detecting human Fibronectin using Simple Western.
Control
HepG2 Whole Cell Lysate
Reviewed Applications
Read 11 Reviews rated 4.2
using
NBP1-91258 in the following applications:

Publications
Read Publications using
NBP1-91258 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 29439094). Equine reactivity reported in scientific literature (PMID: 30450188). Rat reactivity reported in scientific literature (PMID: 30699330).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fibronectin Antibody - BSA Free

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

Fibronectin (FN-EDA, FN, fibronectin 1) is a glycoprotein that is involved in cell adhesion as well as cell migration processes such as embryogenesis, wound healing, host defense and metastasis. Fibronectin appears to be an important factor in the ability of cancerous cells to metastasize by inducing matrix metalloproteinases (MMPs). In a study of human laryngeal carcinoma cells, researchers showed that FN-induced expression of MMP-9 increased the DNA binding activity of transcription factors NF-kappa-B, Activator protein-1 and Specificity protein-1 to the promoter region (PMID: 20638438). Additionally, a study of renal cell cancer showed that the mRNA expression of fibronectin 1 was higher in patients with renal cell cancer as compared to patients with healthy kidneys. This disparity in mRNA expression levels could potentially be used to determine the aggressiveness of renal cell cancer as well as indicate the progression of the disease in patients (PMID: 20860816). Furthermore, a knockdown study of endogenous FN in the A431-III cell line showed a significant decrease of the migratory and invasive activity of the cells as well as an inhibition of secreted MMP-9 (PMID: 21036738).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC

Publications for Fibronectin Antibody (NBP1-91258)(52)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Canine, Equine.

We have publications tested in 7 applications: EM, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
EM
(1)
ICC/IF
(8)
IF/IHC
(6)
IHC
(3)
IHC-Fr
(1)
IHC-P
(11)
WB
(19)
All Applications
Filter By Species
Human
(11)
Mouse
(23)
Rat
(3)
Bovine
(1)
Canine
(3)
Equine
(1)
All Species
Showing Publications 1 - 10 of 52. Show All 52 Publications.
Publications using NBP1-91258 Applications Species
Sung MS, Kim SY, Eom GH, Park SW High VEGF Concentrations Accelerate Human Trabecular Meshwork Fibrosis in a TAZ-Dependent Manner International journal of molecular sciences 2023-06-01 [PMID: 37298577] (WB, Human) WB Human
Barreto R, Carvalho H, Matias G et al. THE EXTRACELLULAR MATRIX PROTEIN PATTERN IN THE CANINE NEOPLASTIC MAMMARY GLAND Tissue and Cell 2023-02-01 [PMID: 36933273] (IHC, Canine) IHC Canine
Wu X, Zhang D, Qiao X et al. Regulating the cell shift of endothelial cell-like myofibroblasts in pulmonary fibrosis The European respiratory journal 2023-06-01 [PMID: 36758986] (WB, Mouse) WB Mouse
Li XF, Selli C, Zhou HL et al. Macrophages promote anti-androgen resistance in prostate cancer bone disease The Journal of experimental medicine 2023-04-03 [PMID: 36749798] (IHC-P, ICC/IF, Mouse)

Details:
Dilution used in IHC-P and ICC 1:500
IHC-P, ICC/IF Mouse
Jung S, Jiang L, Zhao J et al. Clathrin Light Chain Conjugated Drug Delivery for Cancer Bioengineering & Translational Medicine 2021-11-28 [PMID: 36684105] (IHC-Fr, Mouse) IHC-Fr Mouse
Kuczwara V, Schuler G, Pfarrer C et al. Ultrastructural and Immunohistochemical Characterization of Maternal Myofibroblasts in the Bovine Placenta around Parturition Veterinary Sciences 2023-01-07 [PMID: 36669044] (ICC/IF) ICC/IF
Rüegg AB, Kowalewski MP, Ulbrich SE Endometrial extracellular matrix components do not change over the course of embryonic diapause and reactivation in the roe deer (Capreolus capreolus) Reproduction in domestic animals = Zuchthygiene 2023-05-01 [PMID: 36645739] (IHC) IHC
Wu M, Matar DY, Yu Z et al. Continuous NPWT Regulates Fibrosis in Murine Diabetic Wound Healing Pharmaceutics 2022-10-06 [PMID: 36297560] (IHC-P, Mouse) IHC-P Mouse
Gu L, Li A, Lin J et al. Knockdown of SOX9 alleviates tracheal fibrosis through the Wnt/B-catenin signaling pathway Journal of molecular medicine (Berlin, Germany) 2022-11-01 [PMID: 36192639]
Lee GH, Cheon J, Kim D, Jun HS. Lysophosphatidic Acid Promotes Epithelial-Mesenchymal Transition in Kidney Epithelial Cells via the LPAR1/MAPK-AKT/KLF5 Signaling Pathway in Diabetic Nephropathy International Journal of Molecular Sciences 2022-09-10 [PMID: 36142408] (IHC, WB) IHC, WB
Show All 52 Publications.

Reviews for Fibronectin Antibody (NBP1-91258) (11) 4.211

Average Rating: 4.2
(Based on 11 reviews)
We have 11 reviews tested in 4 species: Human, Mouse, Feline, Pig.

Reviews using NBP1-91258:
Filter by Applications
WB
(4)
IHC-Fr
(3)
IF
(1)
Immunocytochemistry-Frozen
(1)
Immunocytochemistry-Paraffin
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(7)
Feline
(1)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Fibronectin NBP1-91258
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3
reviewed by:
Verified Customer
WB Mouse 10/02/2020
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Summary

ApplicationWestern Blot
Sample TestedMouse Pancreas
SpeciesMouse
LotN/A
Western Blot Fibronectin NBP1-91258
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3
reviewed by:
Verified Customer
WB Mouse 10/02/2020
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Summary

ApplicationWestern Blot
Sample TestedPancreas
SpeciesMouse
LotLot B2
Western Blot Fibronectin NBP1-91258
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4
reviewed by:
Verified Customer
WB Human 06/17/2019
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Summary

ApplicationWestern Blot
Sample TestedSVG-A whole cell lysate
SpeciesHuman
LotD3
Immunohistochemistry-Frozen Fibronectin NBP1-91258
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4
reviewed by:
Kirsten Hattermann-Koch
IHC-Fr Mouse 05/09/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse brain
SpeciesMouse
LotD3
Immunohistochemistry-Frozen Fibronectin NBP1-91258
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5
reviewed by:
Verified Customer
IHC-Fr Pig 12/16/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedtrabecular meshwork (TM) region of pig eyes
SpeciesPig
LotD-2
Immunohistochemistry-Frozen Fibronectin NBP1-91258
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4
reviewed by:
Verified Customer
IHC-Fr Feline 04/27/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedcorneal stromal cells
SpeciesFeline
LotD-1
Immunofluorescence Fibronectin NBP1-91258
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5
reviewed by:
Verified Customer
IF Mouse 07/22/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse Kidneys with Alport Disease
SpeciesMouse
LotA-2
Western Blot Fibronectin NBP1-91258
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5
reviewed by:
Verified Customer
WB Mouse 04/07/2016
View

Summary

ApplicationWestern Blot
Sample TestedMouse Atherosclerotic Plaque
SpeciesMouse
LotA-4
Immunocytochemistry-Frozen Fibronectin NBP1-91258
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4
reviewed by:
Lindsey Davis
Immunocytochemistry-Frozen Mouse 06/06/2015
View

Summary

ApplicationImmunocytochemistry-Frozen
SpeciesMouse
Immunocytochemistry-Paraffin Fibronectin NBP1-91258
Enlarge
5
reviewed by:
Verified Customer
Immunocytochemistry-Paraffin Human 04/30/2015
View

Summary

ApplicationImmunocytochemistry-Paraffin
SpeciesHuman
Immunohistochemistry-Paraffin Fibronectin NBP1-91258
Enlarge
4
reviewed by:
Verified Customer
IHC-P Mouse 03/02/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse kidney
SpeciesMouse
LotA-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibronectin Antibody (NBP1-91258) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Fibronectin Products

Research Areas for Fibronectin Antibody (NBP1-91258)

Find related products by research area.

Blogs on Fibronectin.

Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1
By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Recent Reviews

4.2
5
4
4
5
3
2
2
0
1
0

Verified Customer
10/02/2020
Application: WB
Species: Mouse

Verified Customer
10/02/2020
Application: WB
Species: Mouse

Verified Customer
06/17/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol FN1
Entrez
Uniprot