Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in NIH 3T3 cell lysate.
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-91258] - analysis of Fibronectin in human oesophageal cancer tissue using anti-Fibronectin antibody. Image from verified customer review.
Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in HepG2 cell lysate.
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody [NBP1-91258] - Fibronectin antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: Fibronectin Antibody [NBP1-91258] - IHC analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-91258] - analysis of Fibronectin in mouse kidney tissue section using anti-Fibronectin antibody. Image from verified customer review.
Simple Western: Fibronectin/Anastellin Antibody [NBP1-91258] - Simple Western lane view shows a specific band for Fibronectin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using ...read more
In ICC/IF, cytoplasmic staining was observed in HeLa cells. In Western Blot, a band is seen ~262 kDa representing Fibronectin. In IHC-P, staining was observed in the cytoplasm and extracellular space of mouse prostate tissue. In IHC-P, antigen retrieval with 10mM sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Human and Mouse. Based on immunogen's sequence homology, this antibody is predicted to react with Chicken (91%), Rat (95%), Canine/Dog (100%), Bovine (100%) and Equine/Horse (100%). Immunogen similarity with other species: Xenopus (86%) and Newt (82%).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.05% Sodium Azide
Immunogen affinity purified
Alternate Names for Fibronectin Antibody
Fibronectin EDA (FN-EDA, FN, fibronectin 1) is a glycoprotein that is involved in cell adhesion as well as cell migration processes such as embryogenesis, wound healing, host defense and metastasis. Fibronectin appears to be an important factor in the ability of cancerous cells to metastasize by inducing matrix metalloproteinases (MMPs). In a study of human laryngeal carcinoma cells, researchers showed that FN-induced expression of MMP-9 increased the DNA binding activity of transcription factors NF-kappa-B, Activator protein-1 and Specificity protein-1 to the promoter region (PMID: 20638438). Additionally, a study of renal cell cancer showed that the mRNA expression of fibronectin 1 was higher in patients with renal cell cancer as compared to patients with healthy kidneys. This disparity in mRNA expression levels could potentially be used to determine the aggressiveness of renal cell cancer as well as indicate the progression of the disease in patients (PMID: 20860816). Furthermore, a knockdown study of endogenous FN in the A431-III cell line showed a significant decrease of the migratory and invasive activity of the cells as well as an inhibition of secreted MMP-9 (PMID: 21036738).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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