Fibronectin Antibody

Images

 
Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in NIH 3T3 cell lysate.
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells ...read more
Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-91258] - analysis of Fibronectin in human oesophageal cancer tissue using anti-Fibronectin antibody. Image from verified customer review.
Western Blot: Fibronectin Antibody [NBP1-91258] - WB analysis of Fibronectin in HepG2 cell lysate.
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody [NBP1-91258] - Fibronectin antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and ...read more
Immunohistochemistry: Fibronectin Antibody [NBP1-91258] - IHC analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: Fibronectin/Anastellin Antibody [NBP1-91258] - analysis of Fibronectin in mouse kidney tissue section using anti-Fibronectin antibody. Image from verified customer review.
Simple Western: Fibronectin/Anastellin Antibody [NBP1-91258] - Simple Western lane view shows a specific band for Fibronectin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, EqSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Fibronectin Antibody Summary

Immunogen
A synthetic peptide made toward the C-terminal region of the human Fibronectin protein (within residues 2250-2300). [Swiss-Prot P02751]
Localization
Secreted, extracellular space, extracellular matrix.
Marker
Mesenchymal Cells Marker
Predicted Species
Rat (95%), Chicken (91%), Canine (100%), Equine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:40
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
In ICC/IF, cytoplasmic staining was observed in HeLa cells. In Western Blot, a band is seen ~262 kDa representing Fibronectin. In IHC-P, staining was observed in the cytoplasm and extracellular space of mouse prostate tissue. In IHC-P, antigen retrieval with 10mM sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HepG2 Lysate (NBP1-42569)
Reviewed Applications
Read 5 Reviews rated 4.6
using
NBP1-91258 in the following applications:

Publications
Read Publications using
NBP1-91258 in the following applications:

Reactivity Notes

Human and Mouse. Based on immunogen's sequence homology, this antibody is predicted to react with Chicken (91%), Rat (95%), Canine/Dog (100%), Bovine (100%) and Equine/Horse (100%). Immunogen similarity with other species: Xenopus (86%) and Newt (82%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fibronectin Antibody

  • Fibronectin
  • FN1

Background

Fibronectin EDA (FN-EDA, FN, fibronectin 1) is a glycoprotein that is involved in cell adhesion as well as cell migration processes such as embryogenesis, wound healing, host defense and metastasis. Fibronectin appears to be an important factor in the ability of cancerous cells to metastasize by inducing matrix metalloproteinases (MMPs). In a study of human laryngeal carcinoma cells, researchers showed that FN-induced expression of MMP-9 increased the DNA binding activity of transcription factors NF-kappa-B, Activator protein-1 and Specificity protein-1 to the promoter region (PMID: 20638438). Additionally, a study of renal cell cancer showed that the mRNA expression of fibronectin 1 was higher in patients with renal cell cancer as compared to patients with healthy kidneys. This disparity in mRNA expression levels could potentially be used to determine the aggressiveness of renal cell cancer as well as indicate the progression of the disease in patients (PMID: 20860816). Furthermore, a knockdown study of endogenous FN in the A431-III cell line showed a significant decrease of the migratory and invasive activity of the cells as well as an inhibition of secreted MMP-9 (PMID: 21036738).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fibronectin Antibody (NBP1-91258)(5)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ICC/IF, IHC-P, WB.


Filter By Application
ICC/IF
(2)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Mouse
(3)
All Species
Showing Publications 1 - 5 of 5.
Publications using NBP1-91258 Applications Species
Fu M, Song Y, Wen Z et al. Inositol Hexaphosphate and Inositol Inhibit Colorectal Cancer Metastasis to the Liver in BALB/c Mice. Nutrients [PMID: 27187454]
Green CJ, Fraser ST, Day ML. Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro. Hum. Reprod. 2015 Feb 01 [PMID: 25432925] (ICC/IF, Mouse)

Details:
Fibronectin/Anastellin antibody used for ICC-IF on Day 5 blastocysts that were cultured in serum starved medium for 24, 48 or 72 h in the absence or presence of IGF1 or in the presence of a PI3K inhibitor LY294002 or in the presence of IGF1, attached to a coverslip - 15 minute 4% paraformaldehyde fixation, 30 minutes permeablization with PBS-Polyvinyl alcohol-0.3% Triton X100, blocking in PPTB (PBS + PVA + 0.1% Tween-20 + 0.7% BSA), ON 4C incubation of primary, anti-rabbit Alexa 488 secondary antibody (Figure 5).
ICC/IF Mouse
Medina-Ortiz WE, Belmares R, Neubauer S et al. Cellular Fibronectin Expression in Human Trabecular Meshwork and Induction by Transforming Growth Factor-beta2. Invest Ophthalmol Vis Sci. 2013 Oct 17 [PMID: 24030464]
Hosick PA, Alamodi AA, Storm MV et al. Chronic carbon monoxide treatment attenuates development of obesity and remodels adipocytes in mice fed a high-fat diet. Int J Obes (Lond) 2013 Apr 30 [PMID: 23689359] (WB, Mouse) WB Mouse
Wang J, Jiang ZP, Su N et al. The role of peritoneal alternatively activated macrophages in the process of peritoneal fibrosis related to peritoneal dialysi. Int J Mol Sci 2013 May 17 [PMID: 23685870] (IHC-P, ICC/IF, Mouse) IHC-P, ICC/IF Mouse

Reviews for Fibronectin Antibody (NBP1-91258) (5) 4.65

Average Rating: 4.6
(Based on 5 reviews)
We have 5 reviews tested in 2 species: Human, Mouse.
We have 5 reviews tested in 3 application: IF, WB, IHC-P.

Reviews using NBP1-91258:
Filter by Applications
IF
(1)
WB
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(4)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence Fibronectin NBP1-91258
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reviewed by:
Anonymous
IF Mouse 07/22/2016
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Summary

ApplicationImmunofluorescence
Sample TestedMouse Kidneys with Alport Disease
SpeciesMouse
LotA-2

Blocking

Blocking Details5% BSA for 1 hour at Room Temp

Primary Anitbody

Dilution Ratio1:50 over night, 4C

Secondary Antibody

Secondary DescriptionAlexa Fluor Goat anti-Rabbit 555
Secondary Manufacturer Cat#A21428
Secondary Concentration1:500

Details

Detection NotesImmunofluorescence microscopy, a slide without primary antibody was used as a negative control.
Fixation DetailsFixed with 10% Formalin. High pH Citrate Buffer.
Wash Description1X PBS, 3 times for 5 minutes each
Western Blot Fibronectin NBP1-91258
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reviewed by:
Anonymous
WB Mouse 04/07/2016
View

Summary

ApplicationWestern Blot
Sample TestedMouse Atherosclerotic Plaque
SpeciesMouse
LotA-4

Blocking

Blocking Details1% BSA in PBS, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:1000, 1 hour, room temperature, 1%BSA in PBS

Secondary Antibody

Secondary DescriptionBiotinylated Goat anti-rabbit IgG, IRDye 800CW Streptavidin
Secondary Concentration1:1000, 1:1000

Details

Detection NotesLiCor Odyssey CLx
 Fibronectin NBP1-91258
Enlarge
reviewed by:
Lindsey Davis
Mouse 06/06/2015
View

Summary

SpeciesMouse
Special ApplicationsIHC-Fr
FileView PDF
 Fibronectin NBP1-91258
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reviewed by:
Anonymous
Human 04/30/2015
View

Summary

SpeciesHuman
Special Applicationsoesophageal cancer tissue
FileView PDF
Immunohistochemistry-Paraffin Fibronectin NBP1-91258
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reviewed by:
Anonymous
IHC-P Mouse 03/02/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse kidney
SpeciesMouse
LotA-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibronectin Antibody (NBP1-91258) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-91258AF405
Alexa Fluor 488 Labeled NBP1-91258AF488
Alexa Fluor 647 Labeled NBP1-91258AF647
Alexa Fluor 700 Labeled NBP1-91258AF700
Biotin Labeled NBP1-91258B
DyLight 405 Labeled NBP1-91258V
DyLight 488 Labeled NBP1-91258G
DyLight 550 Labeled NBP1-91258R
DyLight 650 Labeled NBP1-91258C
FITC Labeled NBP1-91258F
HRP Labeled NBP1-91258H

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Bioinformatics Tool for Fibronectin Antibody (NBP1-91258)

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Blogs on Fibronectin.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Recent Reviews

4.6
5
3
4
2
3
0
2
0
1
0

Anonymous
07/22/2016
Application: IF
Species: Mouse

Anonymous
04/07/2016
Application: WB
Species: Mouse

Lindsey Davis
06/06/2015
Application:
Species: Mouse

Bioinformatics

Gene Symbol FN1
Entrez
Uniprot