Fibronectin Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were ...read more
Western Blot: Fibronectin Antibody [NBP1-91258] - VSOP observed in perivascular-restricted spinal cord lesions with intact BBB. Immunostaining for laminin (brown) shows vascular endothelium and glia limitans of a ...read more
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.
Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in HepG2 cell lysate.
Western Blot: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in NIH 3T3 cell lysate.
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody [NBP1-91258] - Fibronectin antibody was tested in HeLa cells with DyLight 488 (Green). Nuclei and alpha-tubulin were counterstained with DAPI (Blue) and ...read more
Immunocytochemistry/ Immunofluorescence: Fibronectin Antibody [NBP1-91258] - NIH-3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells ...read more
Immunohistochemistry: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in human renal cancer using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in mouse kidney tissue section using anti-Fibronectin antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Fibronectin Antibody [NBP1-91258] - Analysis of Fibronectin in human oesophageal cancer tissue using anti-Fibronectin antibody. Image from verified customer review.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Feline corneal stromal cells. IHC-Fr and IF (Alexa Fluor488) were performed using NBP1-91258 at 1:400. Image captured by epifluorescent microscopy. Image ...read more
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Trabecular meshwork (TM) region of pig eyes. NBP1-91258 Fibronectin antibody was labelled with Alexa Fluor 488 conjugated secondary antibody (Green). DAPI ...read more
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Mouse brain cryosections were stained with Fibronectin and anti-rabbit Alexa Fluor 555. Magnification 20x. Image from vrified customer review.
Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Staining in mouse carotid artery. Image from a verified customer review.
Simple Western: Fibronectin Antibody [NBP1-91258] - Lane view shows a specific band for Fibronectin in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Eq, FeSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

Fibronectin Antibody - BSA Free Summary

Immunogen
A synthetic peptide made toward the C-terminal region of the human Fibronectin protein (within residues 2250-2300). [Swiss-Prot: P02751]
Localization
Secreted, extracellular space, extracellular matrix.
Marker
Mesenchymal Cells Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:400
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:400
  • Simple Western 1:100
  • Western Blot 1:1000
Application Notes
In Western Blot, a band is seen at ~262 kDa representing Fibronectin. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. In ICC/IF, cytoplasmic staining was observed in HeLa cells. In IHC-P, staining was observed in the cytoplasm and extracellular space of mouse prostate tissue. Antigen retrieval with 10mM sodium citrate buffer (pH 6.0) is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. IHC-F reported in verified customer review. The 12-230kDa separation system and EZ Standard Pack 5 are recommended for detecting human Fibronectin using Simple Western.
Control
HepG2 Whole Cell Lysate
Reviewed Applications
Read 11 Reviews rated 4.2
using
NBP1-91258 in the following applications:

Publications
Read Publications using
NBP1-91258 in the following applications:

Reactivity Notes

Canine reactivity reported in scientific literature (PMID: 29439094). Equine reactivity reported in scientific literature (PMID: 30450188). Rat reactivity reported in scientific literature (PMID: 30699330).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Fibronectin Antibody - BSA Free

  • CIG
  • ED-B
  • fibronectin 1
  • Fibronectin
  • FINC
  • FN
  • FN1
  • FNZ
  • GFND
  • GFND2
  • LETS
  • MSF
  • SMDCF

Background

Fibronectin EDA (FN-EDA, FN, fibronectin 1) is a glycoprotein that is involved in cell adhesion as well as cell migration processes such as embryogenesis, wound healing, host defense and metastasis. Fibronectin appears to be an important factor in the ability of cancerous cells to metastasize by inducing matrix metalloproteinases (MMPs). In a study of human laryngeal carcinoma cells, researchers showed that FN-induced expression of MMP-9 increased the DNA binding activity of transcription factors NF-kappa-B, Activator protein-1 and Specificity protein-1 to the promoter region (PMID: 20638438). Additionally, a study of renal cell cancer showed that the mRNA expression of fibronectin 1 was higher in patients with renal cell cancer as compared to patients with healthy kidneys. This disparity in mRNA expression levels could potentially be used to determine the aggressiveness of renal cell cancer as well as indicate the progression of the disease in patients (PMID: 20860816). Furthermore, a knockdown study of endogenous FN in the A431-III cell line showed a significant decrease of the migratory and invasive activity of the cells as well as an inhibition of secreted MMP-9 (PMID: 21036738).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for Fibronectin Antibody (NBP1-91258)(38)

We have publications tested in 6 confirmed species: Human, Mouse, Rat, Bovine, Canine, Equine.

We have publications tested in 5 applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(5)
IHC
(6)
IHC-Fr
(1)
IHC-P
(9)
WB
(15)
All Applications
Filter By Species
Human
(9)
Mouse
(20)
Rat
(3)
Bovine
(1)
Canine
(2)
Equine
(1)
All Species
Showing Publications 1 - 10 of 38. Show All 38 Publications.
Publications using NBP1-91258 Applications Species
Sterken BA, Ackermann T, MUller C et al. C/EBP beta isoform-specific regulation of migration and invasion in triple-negative breast cancer cells NPJ breast cancer Jan 18 2022 [PMID: 35042889] (WB, Human) WB Human
Carrick FR, Valerio LSA, Gonzalez-Vega MN, Engel D Accelerated Wound Healing Using a Novel Far-Infrared Ceramic Blanket Life Jan 1 2021 [PMID: 34575027] (WB, Mouse) WB Mouse
Wen S, Deng F, Li L et al. VX-765 ameliorates renal injury and fibrosis in diabetes by regulating caspase-1-mediated pyroptosis and inflammation Journal of diabetes investigation Sep 8 2021 [PMID: 34494385] (IHC-P, WB, Mouse, Human) IHC-P, WB Mouse, Human
Geuens T, Ruiter FAA, Schumacher A et al. Thiol-ene cross-linked alginate hydrogel encapsulation modulates the extracellular matrix of kidney organoids by reducing abnormal type 1a1 collagen deposition Biomaterials Jun 19 2021 [PMID: 34198162] (IHC, Human) IHC Human
Chen X, Li C, Liu J et al. Inhibition of ER stress by targeting the IRE1alpha-TXNDC5 pathway alleviates crystalline silica-induced pulmonary fibrosis International immunopharmacology Mar 7 2021 [PMID: 33691254] (IHC-P, Mouse) IHC-P Mouse
Jiang L, Jung S, Zhao J et al. Simultaneous targeting of primary tumor, draining lymph node, and distant metastases through high endothelial venule-targeted delivery Nano Today Feb 1 2021 [PMID: 33391389] (ICC/IF, Mouse)

Details:
Immunofluorescence was performed on mouse cancer cells.
ICC/IF Mouse
Jung S, Jiang L, Zhao J et al. Clathrin Light Chain Conjugated Drug Delivery for Cancer Bioengineering & Translational Medicine Nov 28 2021 (IHC-Fr, Mouse) IHC-Fr Mouse
Liu Y, Zhang H, Wang S et al. Reduced pericyte and tight junction coverage in old diabetic rats are associated with hyperglycemia-induced cerebrovascular pericyte dysfunction American journal of physiology. Heart and circulatory physiology Dec 11 2020 [PMID: 33306445]
Roberts AL, Mavlyutov TA, Perlmutter TE et al. Fibronectin extra domain A (FN-EDA) elevates intraocular pressure through Toll-like receptor 4 signaling Sci Rep Jun 17 2020 [PMID: 32555351] (IHC-P, Mouse) IHC-P Mouse
Wen S, Li S, Li L, Fan Q circACTR2: A Novel Mechanism Regulating High Glucose-Induced Fibrosis in Renal Tubular Cells via Pyroptosis Biol. Pharm. Bull. Mar 3 2020 [PMID: 32115515] (WB, Human) WB Human
Show All 38 Publications.

Reviews for Fibronectin Antibody (NBP1-91258) (11) 4.211

Average Rating: 4.2
(Based on 11 reviews)
We have 11 reviews tested in 4 species: Human, Mouse, Feline, Pig.

Reviews using NBP1-91258:
Filter by Applications
WB
(4)
IHC-Fr
(3)
IF
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(7)
Feline
(1)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Fibronectin NBP1-91258
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3
reviewed by:
Verified Customer
WB Mouse 10/02/2020
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Summary

ApplicationWestern Blot
Sample TestedMouse Pancreas
SpeciesMouse
LotN/A
Western Blot Fibronectin NBP1-91258
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3
reviewed by:
Verified Customer
WB Mouse 10/02/2020
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Summary

ApplicationWestern Blot
Sample TestedPancreas
SpeciesMouse
LotLot B2
Western Blot Fibronectin NBP1-91258
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4
reviewed by:
Verified Customer
WB Human 06/17/2019
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Summary

ApplicationWestern Blot
Sample TestedSVG-A whole cell lysate
SpeciesHuman
LotD3
Immunohistochemistry-Frozen Fibronectin NBP1-91258
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4
reviewed by:
Kirsten Hattermann-Koch
IHC-Fr Mouse 05/09/2019
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested Mouse brain
SpeciesMouse
LotD3
Immunohistochemistry-Frozen Fibronectin NBP1-91258
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5
reviewed by:
Verified Customer
IHC-Fr Pig 12/16/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedtrabecular meshwork (TM) region of pig eyes
SpeciesPig
LotD-2
Immunohistochemistry-Frozen Fibronectin NBP1-91258
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4
reviewed by:
Verified Customer
IHC-Fr Feline 04/27/2018
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Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedcorneal stromal cells
SpeciesFeline
LotD-1
Immunofluorescence Fibronectin NBP1-91258
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5
reviewed by:
Verified Customer
IF Mouse 07/22/2016
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Summary

ApplicationImmunofluorescence
Sample TestedMouse Kidneys with Alport Disease
SpeciesMouse
LotA-2

Blocking

Blocking Details5% BSA for 1 hour at Room Temp

Primary Anitbody

Dilution Ratio1:50 over night, 4C

Secondary Antibody

Secondary DescriptionAlexa Fluor Goat anti-Rabbit 555
Secondary Manufacturer Cat#A21428
Secondary Concentration1:500

Details

Detection NotesImmunofluorescence microscopy, a slide without primary antibody was used as a negative control.
Fixation DetailsFixed with 10% Formalin. High pH Citrate Buffer.
Wash Description1X PBS, 3 times for 5 minutes each
Western Blot Fibronectin NBP1-91258
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5
reviewed by:
Verified Customer
WB Mouse 04/07/2016
View

Summary

ApplicationWestern Blot
Sample TestedMouse Atherosclerotic Plaque
SpeciesMouse
LotA-4

Blocking

Blocking Details1% BSA in PBS, 1 hour, room temperature

Primary Anitbody

Dilution Ratio1:1000, 1 hour, room temperature, 1%BSA in PBS

Secondary Antibody

Secondary DescriptionBiotinylated Goat anti-rabbit IgG, IRDye 800CW Streptavidin
Secondary Concentration1:1000, 1:1000

Details

Detection NotesLiCor Odyssey CLx
 Fibronectin NBP1-91258
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4
reviewed by:
Lindsey Davis
Mouse 06/06/2015
View

Summary

SpeciesMouse
Special ApplicationsIHC-Fr
FileView PDF
 Fibronectin NBP1-91258
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5
reviewed by:
Verified Customer
Human 04/30/2015
View

Summary

SpeciesHuman
Special Applicationsoesophageal cancer tissue
FileView PDF
Immunohistochemistry-Paraffin Fibronectin NBP1-91258
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4
reviewed by:
Verified Customer
IHC-P Mouse 03/02/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse kidney
SpeciesMouse
LotA-2

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Fibronectin Antibody (NBP1-91258) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Fibronectin.

Nickel induces migratory and invasive phenotype in human epithelial cells by epigenetically activating ZEB1
By Jamshed Arslan Pharm.D. Nickel (Ni) is a naturally abundant metallic element. It is a major component of stainless steel, coins, and many other items of daily use. Disturbingly, Ni exposure is associated with can...  Read full blog post.

Bad news for stomach cancer: BAMBI protein inhibits gastric carcinoma via TGF-beta/epithelial-mesenchymal transition signaling
By Jamshed Arslan Pharm.D. Gastric carcinoma is the second leading cause of cancer-related deaths worldwide. One of the key features of gastric carcinoma is acidosis, which promotes growth and metastasis of gastric ...  Read full blog post.

Alpha-smooth muscle actin and the modulation of endothelial and epithelial cell biology
Actin is essential for a wide range of cell functions, ranging from cell division and chromatin remodeling to vesicle trafficking and maintenance of cellular structure. In fact, mislocalization of actin to cell junctions during development leads t...  Read full blog post.

Epithelial-Mesenchymal Transition (EMT) Markers
Epithelial-Mesenchymal Transition (EMT) is the trans-differentiation of stationary epithelial cells into motile mesenchymal cells. During EMT, epithelial cells lose their junctions and apical-basal polarity, reorganize their cytoskeleton, undergo a...  Read full blog post.

Fibronectin: Organizing Cell Activity across the ECM
Fibronectin is a glycoprotein found in the extracellular matrix (ECM) that binds to integrins and other components of the ECM such as collagen and fibrin. Under normal physiological conditions, fibronectin is an important factor in cell adhesion, grow...  Read full blog post.

Using the Laminin Antibody in Angiogenesis Research
Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differe...  Read full blog post.

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Recent Reviews

4.2
5
4
4
5
3
2
2
0
1
0

Verified Customer
10/02/2020
Application: WB
Species: Mouse

Verified Customer
10/02/2020
Application: WB
Species: Mouse

Verified Customer
06/17/2019
Application: WB
Species: Human

Bioinformatics

Gene Symbol FN1
Entrez
Uniprot