Laminin is one of a large number of proteins expressed on the basal laminar of the ECM (extracellular matrix). The laminin antibody database covers several proteins, which interact with integrins and other receptor proteins to support cellular differentiation, morphology, migration, cell survival and the maintenance of tissue phenotypes. Laminin antibody studies have played a key role in research into angiogenesis and tumor development.
The ECM plays an essential role in angiogenesis, providing support for the developing blood vessel and releasing pro- and anti-angiogenic factors which regulate stability and cell survival. During angiogenesis, the ECM basal membrane is degraded, enabling migration of endothelial cells. Although angiogenesis is necessary for wound healing, it is also a feature of metastasis and tumor formation.
The laminins are a family of around 15 trimeric alpha/beta/gamma-chain proteins. Laminin I has been identified as a major promoter of angiogenesis, with endothelial cells shown to differentiate into capillary-like structures on a laminin I-enriched Matrigel matrix. Recent laminin antibody research has identified laminin I to have 20 angiogenic sites, with the α5ß1 and αvß3 integrins identified as surface receptors. These have been shown to specifically target the C16 Y1 chain peptide, which is the only Y1 chain to block laminin I adhesion to collagen and fibronectin. C16 has been shown to be angiogenic in vivo.
We at Novus Biologicals have an extensive antibody database covering laminin research. Our most popular products include a rabbit polyclonal Laminin antibody (NB300-144) and the corresponding active mouse Laminin protein (NBP1-78301) that was used at the immunogen. We also offer excellent sandwich ELISA assays kits for mouse (BEK-2056-2P) and rat (BEK-2055-2P) Laminin. These kits contain 96-well strips pre-coated with a capture laminin antibody; biotinylated polyclonal laminin antibody; an enzyme Avidin-Biotin-Peroxidase complex and a peroxidase substrate, allowing for convenient and accurate analysis of laminin concentrations in sera, plasma, lysates and supernates.
Novus Biologicals offers many Laminin reagents for your research needs including: