The GAPDH Antibody: A ChIP Off the Old Block

Tue, 08/23/2011 - 13:32

GAPDH antibodies are commonly used as a loading control, due to GAPDH's widespread function as a house-keeping protein. As a leading scientific research antibody supplier, Novus Biologicals offer a wide range of GAPDH antibody reagents and support products.

One of the uses for the GAPDH antibody is in double-label immunofluorescence assays, where SiRNA uptake is correlated with down-regulation of target expression. Using a GAPDH antibody, scientists can detect reduced levels of GADPH actuated by SiRNA. In Western Blot, GAPDH antibodies are used to analyse GAPDH in relation to other related proteins, including actin, tubulin, and the amyloid precursor protein.

Chromatin Immunoprecipitation: GAPDH primer

The GAPDH antibody is also commonly used in ChIP (Chromatin Immunoprecipitation) experiments. The ChIP assay was developed by Alexander Varshavsky, et al. in the 1980s, and has been continuously refined since then. Originally, ChIP assays would require a large number of cells and take several days to complete. However, new methods require as few as 100 cells, and can be completed in a single day. The ChIP assay can also be used in combination with other techniques. For example, ChIP-on-chip assays are used to perform genome-wide analysis, and study DNA and protein interactions in vivo.

Recently, we added a number of ChIP primers to our catalog, including a GAPDH primer to complement our ChIP-grade GAPDH antibody reagents. GAPDH Primers amplify DNA in exon 1 of the human GAPDH locus; they contain both forward and reverse primers, and are compatible with all PCR (polymerase chain reaction) chemistries.

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