Catalase Antibody [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Catalase at approximately 64 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Simple Western lane view shows lysates of Exosome Standards (HEK293) (NBP3-11684) and Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Catalase at approximately 61 kDa (as indicated) using 10 µg/ml of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Catalase was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to peroxisomes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF, KO
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Catalase Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Catalase
  Met1-Leu527
  Accession # P04040
• Specificity: Detects human, mouse and rat Catalase in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CAT
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL
  • Knockout Validated
  • Simple Western 5-10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Catalase Antibody [Unconjugated]

• Cas1
• CAT
• Catalase
• Cs-1
• EC 1.11.1.6
• MGC138422
• MGC138424

Background

Cells have evolved complex mechanisms to maintain redox balance and defend against oxidative stress. Catalase is a tetrameric enzyme comprised of four 60 kDa subunits. Catalase is typically localized in the peroxisome where it functions as an antioxidant, protecting cells from damage due to oxidative stress. Catalase converts reactive oxygen species, such as H 2 O 2 into water and O 2 . Human Catalase shares 89% homology to mouse and rat Catalase. The cells redox environment can serve as an important signaling switch or trigger to initiate a number of cellular processes, including gene expression, differentiation, proliferation and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Catalase Antibody (AF3398)(15)

Publications using AF3398

• Soukaina Essadek, Catherine Gondcaille, Stéphane Savary, Mohammad Samadi, Joseph Vamecq, Gérard Lizard et al. Two Argan Oil Phytosterols, Schottenol and Spinasterol, Attenuate Oxidative Stress and Restore LPS-Dysregulated Peroxisomal Functions in Acox1−/− and Wild-Type BV-2 Microglial Cells Antioxidants (Basel) 2023-01-11 [PMID: 36671029]
• Dannah R. Miller, Matthew A. Ingersoll, Yu-Wei Chou, Elizabeth A. Kosmacek, Rebecca E. Oberley-Deegan, Ming-Fong Lin Dynamics of antioxidant heme oxygenase-1 and pro-oxidant p66Shc in promoting advanced prostate cancer progression Free Radical Biology and Medicine 2022-11-01 [PMID: 36265795]
• Soukaina Essadek, Habiba Bouchab, Riad El Kebbaj, Catherine Gondcaille, Soufiane El Kamouni, Stéphane Savary et al. Effects of a Short-Term Lipopolysaccharides Challenge on Mouse Brain and Liver Peroxisomal Antioxidant and beta -oxidative Functions: Protective Action of Argan Oil Pharmaceuticals (Basel) 2022-04-12 [PMID: 35455460]
• MA Farelo, D Korrou-Kar, KF Brooks, TA Russell, K Maringer, PU Mayerhofer Dengue and Zika Virus Capsid Proteins Contain a Common PEX19-Binding Motif Viruses, 2022-01-27;14(2):. 2022-01-27 [PMID: 35215846] (ICC, Human)
• Kohei Hattori, Kenji Tago, Shiori Memezawa, Arisa Ochiai, Sui Sawaguchi, Yukino Kato et al. The Infantile Leukoencephalopathy-Associated Mutation of C11ORF73/HIKESHI Proteins Generates De Novo Interactive Activity with Filamin A, Inhibiting Oligodendroglial Cell Morphological Differentiation Medicines (Basel) 2021-02-01 [PMID: 33535532] (Immunocytochemistry, Mouse)
• A Yammine, A Zarrouk, T Nury, A Vejux, N Latruffe, D Vervandier, M Samadi, JJ Mackrill, H Greige-Ger, L Auezova, G Lizard Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases Cells, 2020-10-23;9(11):. 2020-10-23 [PMID: 33114025] (Western Blot, Mouse)
• P Kouka, F Tekos, Z Papoutsaki, P Stathopoul, M Halabalaki, M Tsantarlio, I Zervos, C Nepka, J Liesivuori, VN Rakitskii, A Tsatsakis, AS Veskoukis, D Kouretas Olive oil with high polyphenolic content induces both beneficial and harmful alterations on rat redox status depending on the tissue Toxicol Rep, 2020-02-13;7(0):421-432. 2020-02-13 [PMID: 32140426] (Western Blot, Rat)
• Changhai Tian, Lie Gao, Andi Zhang, Bryan T. Hackfort, Irving H. Zucker Therapeutic Effects of Nrf2 Activation by Bardoxolone Methyl in Chronic Heart Failure Journal of Pharmacology and Experimental Therapeutics 2019-12-01 [PMID: 31601682] (Western Blot, Rat)
• M Dall, SAJ Trammell, M Asping, AS Hassing, M Agerholm, SG Vienberg, MP Gillum, S Larsen, JT Treebak Mitochondrial function in liver cells is resistant to perturbations in NAD+ salvage capacity J. Biol. Chem., 2019-07-18;0(0):. 2019-07-18 [PMID: 31320478] (Western Blot, Mouse)
• Dannah R. Miller, Matthew A. Ingersoll, Arpita Chatterjee, Brian Baker, Shashank Shrishrimal, Elizabeth A. Kosmacek et al. p66Shc protein through a redox mechanism enhances the progression of prostate cancer cells towards castration-resistance Free Radical Biology and Medicine 2019-08-01 [PMID: 31100478]
• NIKOLAOS GOUTZOURELAS, DIMITRIOS STAGOS, ANASTASIA HOUSMEKERIDOU, CHRISTINA KARAPOULIOU, EFTHALIA KERASIOTI, NEKTARIOS ALIGIANNIS et al. Grape pomace extract exerts antioxidant effects through an increase in GCS levels and GST activity in muscle and endothelial cells International Journal of Molecular Medicine 2015-08-01 [PMID: 26082074]
• Josef Brandauer, Marianne A. Andersen, Holti Kellezi, Steve Risis, Christian Frøsig, Sara G. Vienberg et al. AMP-activated protein kinase controls exercise training- and AICAR-induced increases in SIRT3 and MnSOD Frontiers in Physiology 2015-03-24 [PMID: 25852572]
• J Pajaud, C Ribault, I Ben Mosbah, C Rauch, C Henderson, P Bellaud et al. Glutathione transferases P1/P2 regulate the timing of signaling pathway activations and cell cycle progression during mouse liver regeneration Cell Death & Disease 2015-01-15 [PMID: 25590808]
• Ginet , Vanessa, Puyal , Julien, Rummel , Coralie, Aubry , Dominiqu, Breton , Caroline, Cloux , Anne-Jul, Majjigapu , Somi R, Sordat , Bernard, Vogel , Pierre, Bruzzone , Santina, Nencioni , Alessio, Duchosal , Michel A, Nahimana , Aimable A critical role of autophagy in antileukemia/lymphoma effects of APO866, an inhibitor of NAD biosynthesis. Autophagy, 2014-01-17;10(4):603-17. 2014-01-17 [PMID: 24487122] (Western Blot, Human)
• Kwon IK, Wang R, Thangaraju M PKG inhibits TCF signaling in colon cancer cells by blocking beta-catenin expression and activating FOXO4 Oncogene, 2010-03-29;29(0):3423. 2010-03-29 [PMID: 20348951] (Western Blot, Human)

Reviews for Catalase Antibody (AF3398) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Human, Human and Mouse.

reviewed by: Maksim Baranov WB Human and Mouse 10/01/2019

Summary

• Application: Western Blot
• Sample Tested: Hek 293T cell lysate,RAW264.7 whole cell lysate
• Species: Human and Mouse
• Lot: WVM0314101

reviewed by: Maksim Baranov WB Human 10/01/2019

Summary

• Application: Western Blot
• Sample Tested: HEK 293 cell line,HEK 293 cell lysate,Hek 293T cell lysate,Monocyte-derived dendritic cells,Monocyte-derived immature dendritic cells,RAW264.7 whole cell lysate
• Species: Human
• Lot: WVM0314101

Comments

• Comments: The blot was restained with the goat-anti-Catalase AB (R&D Systems, cat AF3398, Lot WVM0314101) - 1:50 - 2 days 4C (anti-FLAG-bands are from previous staining)
  2nd AB - Donkey-anti-Goat-800 (1:5000) 30 min at RT
  The blot shows clear expression of Catalase by HEKs and human moDC (65kDa band)

reviewed by: Maksim Baranov ICC Human 10/01/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: Monocyte-derived immature dendritic cells
• Species: Human
• Lot: WVM0314101

Bioinformatics

• Gene Symbol: CAT
• Uniprot:
  • P04040()