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Catalase Antibody [Unconjugated]

Images

 
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat ...read more
Catalase was detected in immersion fixed HL‑60 human acute promyelocytic leukemia cell line using Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) at 1.7 µg/mL for 3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Catalase Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Catalase
Met1-Leu527
Accession # P04040
Specificity
Detects human, mouse and rat Catalase in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CAT
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1-15 ug/mL
  • Knockout Validated
  • Simple Western 5 ug/mL
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 3 Reviews rated 5
using
AF3398 in the following applications:

Publications
Read Publications using
AF3398 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Catalase Antibody [Unconjugated]

  • Cas1
  • CAT
  • Catalase
  • Cs-1
  • EC 1.11.1.6
  • MGC138422
  • MGC138424

Background

Cells have evolved complex mechanisms to maintain redox balance and defend against oxidative stress. Catalase is a tetrameric enzyme comprised of four 60 kDa subunits. Catalase is typically localized in the peroxisome where it functions as an antioxidant, protecting cells from damage due to oxidative stress. Catalase converts reactive oxygen species, such as H2O2, into water and O2. Human Catalase shares 89% homology to mouse and rat Catalase. The cells redox environment can serve as an important signaling switch or trigger to initiate a number of cellular processes, including gene expression, differentiation, proliferation and apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, KO

Publications for Catalase Antibody (AF3398)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Rat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF3398 Applications Species
MA Farelo, D Korrou-Kar, KF Brooks, TA Russell, K Maringer, PU Mayerhofer Dengue and Zika Virus Capsid Proteins Contain a Common PEX19-Binding Motif Viruses, 2022;14(2):. 2022 [PMID: 35215846] (ICC, Human) ICC Human
A Yammine, A Zarrouk, T Nury, A Vejux, N Latruffe, D Vervandier, M Samadi, JJ Mackrill, H Greige-Ger, L Auezova, G Lizard Prevention by Dietary Polyphenols (Resveratrol, Quercetin, Apigenin) Against 7-Ketocholesterol-Induced Oxiapoptophagy in Neuronal N2a Cells: Potential Interest for the Treatment of Neurodegenerative and Age-Related Diseases Cells, 2020;9(11):. 2020 [PMID: 33114025] (Western Blot, Mouse) Western Blot Mouse
P Kouka, F Tekos, Z Papoutsaki, P Stathopoul, M Halabalaki, M Tsantarlio, I Zervos, C Nepka, J Liesivuori, VN Rakitskii, A Tsatsakis, AS Veskoukis, D Kouretas Olive oil with high polyphenolic content induces both beneficial and harmful alterations on rat redox status depending on the tissue Toxicol Rep, 2020;7(0):421-432. 2020 [PMID: 32140426] (Western Blot, Rat) Western Blot Rat
M Dall, SAJ Trammell, M Asping, AS Hassing, M Agerholm, SG Vienberg, MP Gillum, S Larsen, JT Treebak Mitochondrial function in liver cells is resistant to perturbations in NAD+ salvage capacity J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31320478] (Western Blot, Mouse) Western Blot Mouse
Ginet , Vanessa, Puyal , Julien, Rummel , Coralie, Aubry , Dominiqu, Breton , Caroline, Cloux , Anne-Jul, Majjigapu , Somi R, Sordat , Bernard, Vogel , Pierre, Bruzzone , Santina, Nencioni , Alessio, Duchosal , Michel A, Nahimana , Aimable A critical role of autophagy in antileukemia/lymphoma effects of APO866, an inhibitor of NAD biosynthesis. Autophagy, 2014;10(4):603-17. 2014 [PMID: 24487122] (Western Blot, Human) Western Blot Human
Kwon IK, Wang R, Thangaraju M PKG inhibits TCF signaling in colon cancer cells by blocking beta-catenin expression and activating FOXO4 Oncogene, 2010;29(0):3423. 2010 [PMID: 20348951] (Western Blot, Human) Western Blot Human

Reviews for Catalase Antibody (AF3398) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Human and Mouse.

Reviews using AF3398:
Filter by Applications
WB
(2)
ICC
(1)
All Applications
Filter by Species
Human
(2)
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Catalase AF3398
Enlarge
5
reviewed by:
Maksim Baranov
WB Human and Mouse 10/01/2019
View

Summary

ApplicationWestern Blot
Sample TestedHek 293T cell lysate,RAW264.7 whole cell lysate
SpeciesHuman and Mouse
LotWVM0314101
Western Blot Catalase AF3398
Enlarge
5
reviewed by:
Maksim Baranov
WB Human 10/01/2019
View

Summary

ApplicationWestern Blot
Sample TestedHEK 293 cell line,HEK 293 cell lysate,Hek 293T cell lysate,Monocyte-derived dendritic cells,Monocyte-derived immature dendritic cells,RAW264.7 whole cell lysate
SpeciesHuman
LotWVM0314101

Comments

CommentsThe blot was restained with the goat-anti-Catalase AB (R&D Systems, cat AF3398, Lot WVM0314101) - 1:50 - 2 days 4C (anti-FLAG-bands are from previous staining)
2nd AB - Donkey-anti-Goat-800 (1:5000) 30 min at RT
The blot shows clear expression of Catalase by HEKs and human moDC (65kDa band)
Immunocytochemistry Catalase AF3398
Enlarge
5
reviewed by:
Maksim Baranov
ICC Human 10/01/2019
View

Summary

ApplicationImmunocytochemistry
Sample TestedMonocyte-derived immature dendritic cells
SpeciesHuman
LotWVM0314101

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Catalase Antibody (AF3398) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Catalase Antibody (AF3398)

Discover related pathways, diseases and genes to Catalase Antibody (AF3398). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for Catalase Antibody (AF3398)

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Pathways for Catalase Antibody (AF3398)

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Blogs on Catalase

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Contact Information

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Calculators

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Maksim Baranov
10/01/2019
Application: WB
Species: Human and Mouse

Maksim Baranov
10/01/2019
Application: WB
Species: Human

Maksim Baranov
10/01/2019
Application: ICC
Species: Human

Bioinformatics

Gene Symbol CAT
Uniprot