MCF-7 Whole Cell Lysate

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

MCF-7 Whole Cell Lysate Summary

Specificity
Total Protein - Human Tumor Cell Line: MCF 7

Applications/Dilutions

Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Breast
Protein State
Native
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for MCF-7 Lysate (NB820-59465) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for MCF-7 Lysate (NB820-59465). (Showing 1 - 1 of 1 FAQ).

  1. We would like to check toxicity of following products. For the following antibodies, could you inform us the concentration of sodium azide? Synaptojanin 1 Antibody (AC1) (cat# NB300-428), Ly-6C Antibody (HK1.4) [Biotin] (cat# NBP1-28048), and Cripto1 Antibody (cat# NB100-1598). For your MCF-7 Whole Cell Lysate (cat# NB820-59465), could you inform us the concentration of EDTA, Glycerol and NP-40?
    • NB300-428 is supplied in 0.02% Sodium Azide NBP1-28048 contains 0.1% Sodium Azide and NB100-1598 has 0.05% sodium azide. With regards to your second question, unfortunately the formula for the lysis buffer is proprietary thus the component concentration is not suppose to be disclosed due to Novus policy.

Other Available Formats

NB820-59465

Additional MCF-7 Products

Array NB820-59465

Blogs on MCF-7

There are no specific blogs for MCF-7, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

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