A-431 Whole Cell Lysate

Images

 
Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stained SDS-PAGE of 40 ug of Human Derived A431 Whole Cell Lysate separated in a 4-20% gradient gel under non-reducing conditions.
Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stain of 0.02 ml of NB 800-PC12 separated in a 4-20% gradient gel under non-reducing conditions (lane 1). MW standard is shown in lane 2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Concentration
1 mg/ml

Order Details

A-431 Whole Cell Lysate Summary

Specificity
A431 whole cell extract lysate

Applications/Dilutions

Application Notes
Ready-to-use A431 Whole Cell Lysate are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOuT dissociating agents. Heat lysate - 95C for 5 minutes and rapidly cool. If dissociating conditions are desired, add reducing agent prior - heating. The recommended loading volume per lane is 10-20 ul depending on the size format of your gel.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X SDS-PAGE Sample Buffer [0.05 mM Tris HCl, 2% SDS, 10% Glycerol, 0.1% bromophenol blue, pH 6.8]
Concentration
1 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Skin
Subcellular Fraction
Whole

Background

This ready-to-use whole cell lysate is derived from a cell line or tissue using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot analysis using antibodies to key cell signaling components to confirm the presence of both high molecular weight and low molecular weight proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Additional A-431 Products

Array NB800-PC12

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