Glucose 6 Phosphate Dehydrogenase Antibody

Images

 
Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Immunoblot analyses of protein expression for Nrf2 and antioxidant enzymes. Representative immunoblots of cytosolic extracts from the hearts of ...read more
Biological Strategies: Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse ...read more
Immunoprecipitation: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of mouse G6PD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IB, IHC, IHC-Fr, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Glucose 6 Phosphate Dehydrogenase Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Glucose-6-Phosphate Dehydrogenase using the numbering given in entry NP_000393.2 (GeneID 2539).
Marker
Cytosol Marker
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
G6PD
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2500
  • Immunoblotting
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 2-10 ug/mg of lysate
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID: 17693254). Use in Immunoblotting reported in scientific literature (PMID 28515695).
Control
Glucose 6 Phosphate Dehydrogenase Overexpression Lysate
Publications
Read Publications using
NB100-236 in the following applications:

  • IB
    1 publication
  • 1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glucose 6 Phosphate Dehydrogenase Antibody

  • G6PD
  • G6PD1EC 1.1.1.49
  • G6PDH
  • glucose-6-phosphate dehydrogenase

Background

Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme that catalyzes the conversion of glucose-6-phosphate into 6-phosphgluconolactone and maintains the levels of the electron donor, NADPH. G6PD is an x-linked housekeeping gene whose deficiency can result in neonatal jaundice, acute hemolysis, or chronic non-spherocytic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-236
Species: Hu, Mu
Applications: WB, IB, IHC, IHC-Fr, IP

Publications for Glucose 6 Phosphate Dehydrogenase Antibody (NB100-236)(17)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IB, IHC-Fr, WB.


Filter By Application
IB
(1)
IHC-Fr
(1)
WB
(2)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-236 Applications Species
Shanmugam G, Narasimhan M, Conley RL et al. Chronic Endurance Exercise Impairs Cardiac Structure and Function in Middle-Aged Mice with Impaired Nrf2 Signaling. Front Physiol May 18 2017 [PMID: 28515695] (IB, Mouse) IB Mouse
Shanmugam G, Narasimhan M, Sakthivel R et al. A biphasic effect of TNF-a in regulation of the Keap1/Nrf2 pathway in cardiomyocytes Redox Biol 2016 Jun 27 [PMID: 27423013]
Gounder SS, Kannan S, Devadoss D et al. Impaired Transcriptional Activity of Nrf2 in Age-Related Myocardial Oxidative Stress Is Reversible by Moderate Exercise Training PLoS One 2012 [PMID: 23029187] (WB, Mouse) WB Mouse
Huang Y, Bell LN, Okamura J et al. Phospho-deltaNp63alpha/SREBF1 protein interactions: Bridging cell metabolism and cisplatin chemoresistance Cell Cycle 2012 Sep 5 [PMID: 22951905] (WB, Human) WB Human
Jeon SM, Chandel NS, Hay N et al. AMPK regulates NADPH homeostasis to promote tumour cell survival during energy stress. Nature 2012 May [PMID: 22660331]
Spencer NY, Yan Z, Boudreau RL et al. Control of hepatic nuclear superoxide production by glucose 6-phosphate dehydrogenase and NADPH oxidase-4. J Biol Chem 2011 Mar [PMID: 21212270]
Zhang Z, Liew CW, Handy DE et al. High glucose inhibits glucose-6-phosphate dehydrogenase, leading to increased oxidative stress and beta-cell apoptosis. FASEB J 2010 May [PMID: 20032314]
Pan S, World CJ, Kovacs CJ et al. Glucose 6-phosphate dehydrogenase is regulated through c-Src-mediated tyrosine phosphorylation in endothelial cells. Arterioscler Thromb Vasc Biol 2009 Jun [PMID: 19359662]
Maloyan A, Osinska H, Lammerding J et al. Biochemical Mechanical Dysfunction in a Mouse Model of Desmin-Related Myopathy. CIRCRESAHA.108.193516. 2009 [PMID: 19299643]
Orr AL, Li S, Wang C-E et al. N-Terminal Mutant Huntingtin Associates with Mitochondria Impairs Mitochondrial Trafficking. J Neurosci;28(11):2783-92. March 12 2008. [PMID: 18337408]
Show All 17 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Glucose 6 Phosphate Dehydrogenase Antibody (NB100-236) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-236AF350
Alexa Fluor 405 NB100-236AF405
Alexa Fluor 488 NB100-236AF488
Alexa Fluor 532 NB100-236AF532
Alexa Fluor 594 NB100-236AF594
Alexa Fluor 647 NB100-236AF647
Alexa Fluor 700 NB100-236AF700
Alexa Fluor 750 NB100-236AF750
Biotin NB100-236B
DyLight 350 NB100-236UV
DyLight 405 NB100-236V
DyLight 488 NB100-236G
DyLight 550 NB100-236R
DyLight 594 NB100-236DL594
DyLight 650 NB100-236C
DyLight 680 NB100-236FR
DyLight 755 NB100-236IR
FITC NB100-236F
HRP NB100-236H
Janelia Fluor 549 NB100-236JF549
Janelia Fluor 646 NB100-236JF646

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Bioinformatics

Gene Symbol G6PD