Glucose 6 Phosphate Dehydrogenase Antibody

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Immunoblot analyses of protein expression for Nrf2 and antioxidant enzymes. Representative immunoblots of cytosolic extracts from the hearts of young and old mice under basal conditions and following EES. Protein blots were probed with anti-HO1, NQO1, GCLM, GCLC, Catalase, SOD1, SOD2, GSR, G6PD, GPX1 and GAPDH. Individual lanes indicate a single animal. Densitometry analysis of respective protein signals was performed using Image-J and expressed as relative intensity units calculated as mean values of young and old, *p<0.05. Individual lanes indicate each animal (n?=?6). #p<0.05-between basal and EES. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0045697), licensed under a CC-BY license.

Western Blot: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of Human and Mouse G6PD by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G6PD antibody NB100-236 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: Glucose 6 Phosphate Dehydrogenase Antibody [NB100-236] - Detection of mouse G6PD by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-G6PD antibody NB100-236 (lot 2) used for IP at 6 ug per reaction. G6PD was also immunoprecipitated by a previous lot of this antibody (lot 1). For blotting immunoprecipitated G6PD, NB100-236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Glucose 6 Phosphate Dehydrogenase Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 50 and 100 of human Glucose-6-Phosphate Dehydrogenase using the numbering given in entry NP_000393.2 (GeneID 2539).
• Marker: Cytosol Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: G6PD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 2-10 ug/mg of lysate
  • Western Blot 1:500-1:2500
• Application Notes: Use in IHC-Frozen reported in scientific literature (PMID: 17693254). Use in Immunoblotting reported in scientific literature (PMID 28515695).
• Control:

  Glucose 6 Phosphate Dehydrogenase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Glucose 6 Phosphate Dehydrogenase Antibody

• G6PD
• G6PD1EC 1.1.1.49
• G6PDH
• glucose-6-phosphate dehydrogenase

Background

Glucose-6-phosphate dehydrogenase (G6PD) is a cytosolic enzyme that catalyzes the conversion of glucose-6-phosphate into 6-phosphgluconolactone and maintains the levels of the electron donor, NADPH. G6PD is an x-linked housekeeping gene whose deficiency can result in neonatal jaundice, acute hemolysis, or chronic non-spherocytic hemolytic anemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glucose 6 Phosphate Dehydrogenase Antibody (NB100-236)(17)

Publications using NB100-236

• Shanmugam G, Narasimhan M, Conley RL et al. Chronic Endurance Exercise Impairs Cardiac Structure and Function in Middle-Aged Mice with Impaired Nrf2 Signaling. Front Physiol 2017-05-18 [PMID: 28515695] (IB, Mouse)
• Shanmugam G, Narasimhan M, Sakthivel R et al. A biphasic effect of TNF-a in regulation of the Keap1/Nrf2 pathway in cardiomyocytes Redox Biol 2016-06-27 [PMID: 27423013]
• Gounder SS, Kannan S, Devadoss D et al. Impaired Transcriptional Activity of Nrf2 in Age-Related Myocardial Oxidative Stress Is Reversible by Moderate Exercise Training PLoS One 2012-01-01 [PMID: 23029187] (WB, Mouse)
• Huang Y, Bell LN, Okamura J et al. Phospho-deltaNp63alpha/SREBF1 protein interactions: Bridging cell metabolism and cisplatin chemoresistance Cell Cycle 2012-09-05 [PMID: 22951905] (WB, Human)
• Jeon SM, Chandel NS, Hay N et al. AMPK regulates NADPH homeostasis to promote tumour cell survival during energy stress. Nature 2012-05-01 [PMID: 22660331]
• Spencer NY, Yan Z, Boudreau RL et al. Control of hepatic nuclear superoxide production by glucose 6-phosphate dehydrogenase and NADPH oxidase-4. J Biol Chem 2011-03-01 [PMID: 21212270]
• Zhang Z, Liew CW, Handy DE et al. High glucose inhibits glucose-6-phosphate dehydrogenase, leading to increased oxidative stress and beta-cell apoptosis. FASEB J 2010-05-01 [PMID: 20032314]
• Pan S, World CJ, Kovacs CJ et al. Glucose 6-phosphate dehydrogenase is regulated through c-Src-mediated tyrosine phosphorylation in endothelial cells. Arterioscler Thromb Vasc Biol 2009-06-01 [PMID: 19359662]
• Maloyan A, Osinska H, Lammerding J et al. Biochemical Mechanical Dysfunction in a Mouse Model of Desmin-Related Myopathy. Circ Res;104(8):1021-1028. 2009-01-01 [PMID: 19299643]
• Orr AL, Li S, Wang CE et al. N-terminal mutant huntingtin associates with mitochondria and impairs mitochondrial trafficking. J Neurosci 2008-03-12 [PMID: 18337408]
• Iwashima F, Yoshimoto T, Minami I et al. Aldosterone induces superoxide generation via Rac1 activation in endothelial cells. Endocrinology 2008-03-01 [PMID: 18079208]
• Rajasekaran NS, Connell P, Christians ES et al. Human alpha B-crystallin mutation causes oxido-reductive stress and protein aggregation cardiomyopathy in mice. Cell 2007-08-10 [PMID: 17693254] (IHC-Fr, Mouse)
• Funes JM, Quintero M, Henderson S et al. Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production. Proc Natl Acad Sci U S A 2007-04-01 [PMID: 17384149]
• Matsui R, Xu S, MaitlKA et al. Glucose-6-phosphate dehydrogenase deficiency decreases vascular superoxide and atherosclerotic lesions in apolipoprotein E(-/-) mice. Arterioscler Thromb Vasc Biol 2006-04-01 [PMID: 16439706]
• Matsui R, Xu S, MaitlKA et al. Glucose-6 phosphate dehydrogenase deficiency decreases the vascular response to angiotensin II. Circulation 2005-07-01 [PMID: 15998684]
• Xu Y, Osborne BW, Stanton RC et al. Diabetes causes inhibition of glucose-6-phosphate dehydrogenase via activation of PKA, which contributes to oxidative stress in rat kidney cortex. Am J Physiol Renal Physiol 2005-11-01 [PMID: 15956780]
• Jain M, Cui L, Brenner DA et al. Increased myocardial dysfunction after ischemia-reperfusion in mice lacking glucose-6-phosphate dehydrogenase. Circulation 2004-02-01 [PMID: 14757696]

Bioinformatics

• Gene Symbol: G6PD