GAPDH-2 Antibody (2E3-2E10)

Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.

Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.

• Reactivity: Hu, Ec
• Applications: WB, ELISA, IHC, IHC-P, S-ELISA
• Clone: 2E3-2E10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GAPDH-2 Antibody (2E3-2E10) Summary

• Immunogen: GAPDS (AAH36373, 1 a.a. ~ 408 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSKRDIVLTNVTVVQLLRQPCPVTRAPPPPEPKAEVEPQPQPEPTPVREEIKPPPPPLPPHPATPPPKMVSVARELTVGINGFGRIGRLVLRACMEKGVKVVAVNDPFIDPEYMVYMFKYDSTHGRYKGSVEFRNGQLVVDNHEISVYQCKEPKQIPWRAVGSPYVVESTGVYLSIQAASDHISAGAQRVVISAPSPDAPMFVMGVNENDYNPGSMNIVSNASCTTNCLAPLAKVIHERFGIVEGLMTTVHSYTATQKTVDGPSRKAWRDGRGAHQNIIPASTGAAKAVTKVIPELKGKLTGMAFRVPTPDVSVVDLTCRLAQPAPYSAIKEAVKAAAKGPMAGILAYTEDEVVSTDFLGDTHSSIFDAKAGIALNDNFVKLISWYDNEYGYSHRVVDLLRYMFSRDK
• Specificity: GAPDHS - glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GAPDHS
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GAPDH-2 Antibody (2E3-2E10)

• EC 1.2.1
• EC 1.2.1.12
• GAPD2
• GAPD2HSD-35
• GAPDH2
• GAPDH-2
• GAPDH-2glyceraldehyde-3-phosphate dehydrogenase, testis-specific
• GAPDHS
• GAPDS
• glyceraldehyde-3-phosphate dehydrogenase, spermatogenic
• HSD-35
• Spermatogenic cell-specific glyceraldehyde 3-phosphate dehydrogenase 2
• Spermatogenic glyceraldehyde-3-phosphate dehydrogenase

Background

This gene encodes a protein belonging to the glyceraldehyde-3-phosphate dehydrogenase family of enzymes that play an important role in carbohydrate metabolism. Like its somatic cell counterpart, this sperm-specific enzyme functions in a nicotinamide adenine dinucleotide-dependent manner to remove hydrogen and add phosphate to glyceraldehyde 3-phosphate to form 1,3-diphosphoglycerate. During spermiogenesis, this enzyme may play an important role in regulating the switch between different energy-producing pathways, and it is required for sperm motility and male fertility.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GAPDH-2 Antibody (H00026330-M01)(11)

Publications using H00026330-M01

• Kim S, Hata A, Kang H. Down-Regulation of miR-96 by Bone Morphogenetic Protein Signaling is Critical for Vascular Smooth Muscle Cell Phenotype Modulation. J Cell Biochem. 2013-12-04 [PMID: 24375867]
• Kang H, Louie J, Weisman A et al. Inhibition of microRNA-302 (miR-302) by bone morphogenetic protein 4 (BMP4) facilitates the BMP signaling pathway. J Biol Chem. 2012-09-17 [PMID: 22988237]
• Kuravsky ML, Schmalhausen EV, Pozdnyakova NV, Muronetz VI. Isolation of antibodies against different protein conformations using immunoaffinity chromatography. Anal Biochem. 2012-04-03 [PMID: 22484035]
• Kang H, Davis-Dusenbery BN, Nguyen PH et al. Bone morphogenetic protein 4 promotes vascular smooth muscle contractility by activating miR-21, which downregulates expression of the family of Dedicator of Cytokinesis (DOCK) proteins. J Biol Chem. 2011-12-09 [PMID: 22158624]
• Davis-Dusenbery BN, Chan MC, Reno KE et al. Down-regulation of KLF4 by MIR-143/145 is critical for modulation of vascular smooth muscle cell phenotype by TGF-{beta} and BMP. J Biol Chem. 2011-06-13 [PMID: 21673106]
• Chan MC, Weisman AS, Kang H et al. The amiloride derivative phenamil attenuates pulmonary vascular remodeling by activating NFAT and the BMP signaling pathway. Mol Cell Biol. 2010-12-06 [PMID: 21135135]
• Davis BN, Hilyard AC, Nguyen PH et al. Smad proteins bind a conserved RNA sequence to promote microRNA maturation by Drosha. Mol Cell. 2010-08-13 [PMID: 20705240]
• Chan MC, Hilyard AC, Wu C et al. Molecular basis for antagonism between PDGF and the TGFbeta family of signalling pathways by control of miR-24 expression. EMBO J 29(3):559-73. 2010-02-03 [PMID: 20019669]
• Davis BN, Hilyard AC, Nguyen PN et al. Induction of microrna-221 by platelet-derived growth factor signaling is critical for modulation of vascular smooth muscle phenotype. J Biol Chem. 2008-12-16 [PMID: 19088079]
• Davis BN, Hilyard AC, Lagna G et al. SMAD proteins control DROSHA-mediated microRNA maturation. Nature;454(7200):56-61. 2008-07-03 [PMID: 18548003]
• Shchutskaya YY, Elkina YL, Kuravsky ML et al. Investigation of glyceraldehyde-3-phosphate dehydrogenase from human sperms. Biochemistry (Mosc);73(2):185-91. 2008-02-01 [PMID: 18298375]

Bioinformatics

• Gene Symbol: GAPDHS
• Entrez:
  • 26330(Human)
• OMIM:
  • 609169(Human)
• Uniprot:
  • AAH36373(Human)