GAPDH Products

Antibodies
GAPDH Antibody (1D4)
GAPDH Antibody (1D4)
Species: Hu, Mu, Rt, Po, Av, Bv, Ch, Eq, Ha, Op, Pm, Sh, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IP, ChIP
Host: Mouse Monoclonal
GAPDH Antibody
GAPDH Antibody
NB100-56875
Species: Hu, Mu, Rt, Po, Dr, Fe, Pm
Applications: WB, Simple Western
Host: Rabbit Polyclonal
Lysates
GAPDH Overexpression Lysate ( ...
GAPDH Overexpression Lysate (Denat...
H00002597-T01
Species: Hu
Applications: WB
GAPDH Overexpression Lysate ( ...
GAPDH Overexpression Lysate (Native)
NBL1-10967
Applications: WB
Proteins
GAPDH Recombinant Protein
GAPDH Recombinant Protein
H00002597-P02
Species: Hu
Applications: WB, ELISA, PA
GAPDH Recombinant Protein
GAPDH Recombinant Protein
H00002597-P01
Species: Hu
Applications: WB, ELISA, PA
GAPDH Partial Recombinant Pro ...
GAPDH Partial Recombinant Protein
H00002597-Q01
Species: Hu
Applications: WB, ELISA, PA
RNAi
GAPDH RNAi
GAPDH RNAi
H00002597-R01
Species: Hu
Applications: RNAi, RNAi SP
GAPDH RNAi
GAPDH RNAi
R0016
Species: Hu
Applications: RNAi, RNAi SP

Description

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is is a 146 kDa tetramer metabolic enzyme primarily responsible for catalyzing the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate in the glycolytic pathway. Apart from a role in glycolysis, GAPDH may have other roles such as in the activation of transcription.

GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein (mutations in which cause some forms of Alzheimer's disease) and the polyglutamine tracts of Huntingtin (the protein product, aberrant forms of which are causative of Huntington's disease). Associations with actin and tubulin have also been reported. GAPDH protein may also have a role in the regulation of apoptosis, and interestingly migrates from the cytoplasm into the nucleus when cells become apoptotic.

GAPDH is considered a "house keeping gene" because it is expressed in large amounts and is required at all times for an array of important cell maintainece functions. Therefore, GAPDH mRNA levels are often used as standards in studies of mRNA expression. Additionally, scientists may use specific antibodies to GAPDH as loading controls for western blot experiments.

Bioinformatics

Entrez Human
Mouse
Rat
Bovine
Rabbit
Uniprot Human
Human
Human
Human
Human
Human
Human
Mouse
Rat
Porcine
Bovine
Product By Gene ID 2597
Alternate Names
  • EC 1.2.1
  • MGC88685
  • EC 2.6.99.-
  • G3PD
  • glyceraldehyde 3-phosphate dehydrogenase
  • EC 1.2.1.12
  • aging-associated gene 9 protein
  • GAPDPeptidyl-cysteine S-nitrosylase GAPDH
  • glyceraldehyde-3-phosphate dehydrogenase

Bioinformatics Tool for GAPDH

Discover related pathways, diseases and genes to GAPDH. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
 
Vizit™, under license from BioVista Inc.

Related GAPDH Blog Posts

Check out the latest blog posts on GAPDH.
Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...    Read more.
GAPDH - A "housekeeping" gene with diverse functions in cellular homeostasis
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing west blots. However, this pri...    Read more.
A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...    Read more.
GAPDH: More than a housekeeping gene
GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway.  Apart from its role in gl...    Read more.
GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase)
GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin...    Read more.
Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...    Read more.
Beta Actin and GAPDH: The Importance of Loading Controls
Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. A loading control anti...    Read more.
The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...    Read more.
New Primers Available for ChIP Procedures
Novus has recently released six new primer sets designed for use in real-time PCR DNA amplification of housekeeping, silent, or heterochromatin associated proteins. Specifically, the primers are for GAPDH, RPL30, MyoD1, AFM, alpha Satellite, and Satel...    Read more.
The GAPDH Antibody: A ChIP Off the Old Block
GAPDH antibodies are commonly used as a loading control, due to GAPDH's widespread function as a house-keeping protein. As a leading scientific research antibody supplier, Novus Biologicals offer a wide range of GAPDH antibody reagents and support pro...    Read more.
The GAPDH Antibody: A Diverse Area of Research
The glyceraldehyde 3-phosphate dehydrogenase, or GAPDH enzyme plays an important role in the conversion of glucose for energy, catalyzing the sixth step of the glycolytic pathway. A common and widely expressed protein, GAPDH mRNA is often used as a st...    Read more.
Read more GAPDH related blogs.