GAPDH Products

Antibodies
GAPDH Antibody (1D4)
GAPDH Antibody (1D4)
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Dr, Eq, Fe, Ha, Ma-Op, Pm, Rb, Sh, Ze
Applications: WB, Simple Western, ICC/IF, IHC, IP, ChIP, KD, Single-Cell Western
Host: Mouse Monoclonal
GAPDH Antibody
GAPDH Antibody
Species: Hu, Mu, Rt, Po, Ca, Dr, Fe, Ha, Pm, Ma
Applications: WB, Simple Western, ICC/IF, IHC
Host: Rabbit Polyclonal
GAPDH Antibody [Unconjugated] ...
GAPDH Antibody [Unconjugated]
2275-PC-100
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IP
Host: Rabbit Polyclonal
ELISA Kits
Human/Mouse/Rat Total GAPDH D ...
Human/Mouse/Rat Total GAPDH DuoSet...
DYC5718-2
Species: Hu, Mu, Rt
Applications: ELISA
Conjugate Catalog # Availability Size Price
Formulation Catalog # Availability Price  
Lysates
GAPDH Overexpression Lysate
GAPDH Overexpression Lysate
NBL1-10967
Species: Hu
Applications: WB
Proteins
Recombinant Human GAPDH GST ( ...
Recombinant Human GAPDH GST (N-Ter...
H00002597-P02
Species: Hu
Applications: WB, ELISA, MA, PAGE, AP
Recombinant Human GAPDH Prote ...
Recombinant Human GAPDH Protein
NBP2-52615
Species: Hu
Applications: PAGE, Enzyme Activity
Recombinant Mouse GAPDH His P ...
Recombinant Mouse GAPDH His Protein
NBP2-52622
Species: Mu
Applications: PAGE, Enzyme Activity

Description

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References

1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968



Bioinformatics

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Alternate Names
  • aging-associated gene 9 protein
  • EC 1.2.1
  • EC 1.2.1.12
  • EC 2.6.99.-
  • G3PD
  • GAPD
  • glyceraldehyde 3-phosphate dehydrogenase
  • glyceraldehyde-3-phosphate dehydrogenase
  • MGC88685
  • Peptidyl-cysteine S-nitrosylase GAPDH