GAPDH Products

Antibodies
GAPDH Antibody (1D4)
GAPDH Antibody (1D4)
NB300-221
Species: Hu, Mu, Rt, Po, Av, Bv, Ca, Ch, ChHa, Eq, Fe, Ha, Op, Pm, Rb, Sh, Ze
Applications: WB, Simple Western, ChIP, ICC/IF, IHC, IHC-Fr, IP, KD, Single Cell Western
Host: Mouse Monoclonal
GAPDH Antibody
GAPDH Antibody
NB100-56875
Species: Hu, Mu, Rt, Ca, Dr, Fe, Ha, Pm, Po, Ge
Applications: WB, Simple Western, IB
Host: Rabbit Polyclonal
Lysates
GAPDH Overexpression Lysate
GAPDH Overexpression Lysate
NBL1-10967
Species: Hu
Applications: WB
Proteins
Recombinant Human GAPDH Prote ...
Recombinant Human GAPDH Protein
H00002597-P02
Species: Hu
Applications: WB, ELISA, PA, PAGE, AP
Recombinant Human GAPDH Prote ...
Recombinant Human GAPDH Protein
NBP2-52615
Species: Hu
Applications: Func, PAGE, Bioactivity
Recombinant Mouse GAPDH Prote ...
Recombinant Mouse GAPDH Protein
NBP2-52622
Species: Mu
Applications: Func, PAGE, Bioactivity

Description

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis, converting glyceraldehyde-3-phosphate into 1,3 diphosphoglycerate. This constitutively expressed, homotetramer protein can be found in the nucleus and cytoplasm, and the monomer has a theoretical molecular weight of 36 kDa. Known as a housekeeping gene, GAPDH routinely serves as a control for real-time PCR (RT-PCR) and as a loading control for Western Blots (1-3). In addition to its role in glycolysis, GAPDH has multiple cellular functions including membrane trafficking, transcription activation, apoptosis, DNA repair, and has been implicated in various pathologies such as cancer and neurodegenerative diseases including Alzheimer's and Huntington's (4,5).

References
1) Barber RD, Harmer DW, Coleman RA, Clark BJ. (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics. 21(3):389-95. PMID: 15769908

2) Jia Y, Takimoto K. (2006) Mitogen-activated protein kinases control cardiac KChIP2 gene expression. Circ Res. 98(3):386-93. PMID: 16385079

3) Godsel LM, Hsieh SN, Amargo EV, Bass AE, Pascoe-McGillicuddy LT, Huen AC, Thorne ME, Gaudry CA, Park JK, Myung K, Goldman RD, Chew TL, Green KJ. (2005) Desmoplakin assembly dynamics in four dimensions: multiple phases differentially regulated by intermediate filaments and actin. J Cell Biol. 171(6):1045-59. PMID: 16365169

4) Sirover MA1. (1999) New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase. Biochim Biophys Acta. 1432(2): 159-84. PMID: 10407139

5) Tristan C, Shahani N, Sedlak TW, Sawa A. (2011) The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 23(2):317-23. PMID: 20727968



Bioinformatics

Entrez Human
Mouse
Rat
Bovine
Rabbit
Uniprot Human
Human
Human
Human
Human
Human
Human
Mouse
Rat
Porcine
Bovine
Product By Gene ID 2597
Alternate Names
  • Peptidyl-cysteine S-nitrosylase GAPDH
  • GAPD
  • EC 1.2.1
  • MGC88685
  • EC 2.6.99.-
  • G3PD
  • glyceraldehyde 3-phosphate dehydrogenase
  • EC 1.2.1.12
  • aging-associated gene 9 protein
  • glyceraldehyde-3-phosphate dehydrogenase

Bioinformatics Tool for GAPDH

Discover related pathways, diseases and genes to GAPDH. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
 
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Related GAPDH Blog Posts

Check out the latest blog posts on GAPDH.
Considerations for Quantitative Western blotting
By Jamshed Arslan, Pharm. D., PhD. Since its inception in 1979, Western blotting has undergone several developments. The use of radioactive probes was common throughout 1980s, but utilizing secondary antibodies labell...    Read more.
The use of Beta Actin (AC-15) as a loading control across multiple species
Actin is a fundamental component of the cytoskeleton, where it has the ability to create and break down actin filament formation in response to various cell needs.  Actin has six highly conserved isoforms, however beta and gamma actin are the two...    Read more.
Tips on choosing an ideal loading control antibody for Western Blotting
Western blotting is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. When p...    Read more.
GAPDH - A "housekeeping" gene with diverse functions in cellular homeostasis
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a well-known housekeeping gene with functions in glycolysis. Many biologists are familiar with the gene and use GAPDH antibodies for a loading control when performing western blots. However, this...    Read more.
A New Standard in Antibody Testing - Simple Western Certified Antibodies
The Western blot is one of the most commonly used antibody assay techniques in cell and molecular biology research since its development over three decades ago, and is considered the gold standard for protein detection and quantification. The tradi...    Read more.
GAPDH: More than a housekeeping gene
GAPDH is a 146kD tetramer glycolytic pathway metabolic enzyme composed of four 30-40 kDa subunits. It is responsible for reversibly phosphorylating its substrate glyceraldehyde 3-phosphate within the glycolytic pathway.  Apart from its role in gl...    Read more.
GAPDH (Glyceraldehyde 3-Phosphate Dehydrogenase)
GAPDH is a 146 kD tetramer glycolytic pathway metabolic enzyme responsible for reversibly phosphorylating glyceraldehyde 3-phosphate. It may have other possible functions in transcriptional activation. GAPDH is highly expressed due to this housekeepin...    Read more.
Time to Start Actin Like a Reliable 'Housekeeper'!
A growing body of data and studies using actin antibodies supports a view of the actin cytoskeleton of smooth muscle cells as a dynamic structure that plays an integral role in regulating the development of mechanical tension and the material properti...    Read more.
Beta Actin and GAPDH: The Importance of Loading Controls
Western blotting is an essential technique to probe protein expression in complex cell or tissue lysates. To accurately determine protein expression and interpret Western blot results, it is important to use loading controls. A loading control anti...    Read more.
The GAPDH Antibody in Western blot Assays
The loading controls on our antibody database are widely used in gel electrophoresis and Western blotting studies. Products like the GAPDH antibody detect "housekeeping" proteins which are abundantly distributed in cells. This makes them useful for ch...    Read more.
New Primers Available for ChIP Procedures
Novus has recently released six new primer sets designed for use in real-time PCR DNA amplification of housekeeping, silent, or heterochromatin associated proteins. Specifically, the primers are for GAPDH, RPL30, MyoD1, AFM, alpha Satellite, and Satel...    Read more.
The GAPDH Antibody: A ChIP Off the Old Block
GAPDH antibodies are commonly used as a loading control, due to GAPDH's widespread function as a house-keeping protein. As a leading scientific research antibody supplier, Novus Biologicals offer a wide range of GAPDH antibody reagents and support pro...    Read more.
The GAPDH Antibody: A Diverse Area of Research
The glyceraldehyde 3-phosphate dehydrogenase, or GAPDH enzyme plays an important role in the conversion of glucose for energy, catalyzing the sixth step of the glycolytic pathway. A common and widely expressed protein, GAPDH mRNA is often used as a st...    Read more.
Read more GAPDH related blogs.