Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate ...read more
Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody [NBP1-31470] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NBP1-31470] - Paraffin-embedded SNU-16 xenograft, using antibody at 1:100 dilution.
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Sample(30 ug of whole cell lysate) A:A431 B:H1299 12% SDS PAGE, antibody diluted at 1:2000.
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell ...read more
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract.
Flow usage reported in scientific literature (PMID: 22262862).
31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read 1 Review rated 5 using NBP1-31470 in the following applications:
Expected cross reactivity based on sequence homology: Yeast (83%), Chimpanzee (100%), Xenopus Tropicalis (92%).
Packaging, Storage & Formulations
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
PBS (pH 7.0) and 20% Glycerol
0.025% Proclin 300
Immunogen affinity purified
Alternate Names for Triosephosphate isomerase Antibody
triosephosphate isomerase 1
Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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