Triosephosphate isomerase Antibody

Images

 
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate ...read more
Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody [NBP1-31470] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NBP1-31470] - Paraffin-embedded SNU-16 xenograft, using antibody at 1:100 dilution.
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Sample(30 ug of whole cell lysate) A:A431 B:H1299 12% SDS PAGE, antibody diluted at 1:2000.
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell ...read more
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Mk, XpSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
     

Genetic Strategies

   

Order Details

Triosephosphate isomerase Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.
Predicted Species
Xenopus (92%), Bovine (100%), Monkey (100%), Canine (100%), Chicken (92%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
Flow usage reported in scientific literature (PMID: 22262862).
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31470 in the following applications:

Publications
Read Publications using NBP1-31470.

Reactivity Notes

Expected cross reactivity based on sequence homology: Yeast (83%), Chimpanzee (100%), Xenopus Tropicalis (92%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Triosephosphate isomerase Antibody

  • EC 5.3.1.1
  • MGC88108
  • TIM
  • TPI
  • triosephosphate isomerase 1
  • triosephosphate isomerase
  • Triose-phosphate isomerase

Background

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Triosephosphate isomerase Antibody (NBP1-31470)(2)

We have publications tested in 1 confirmed species: Virus.


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Review for Triosephosphate isomerase Antibody (NBP1-31470) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-31470:
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WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Western Blot Triosephosphate isomerase NBP1-31470
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reviewed by:
Anonymous
WB Other 09/21/2015
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Summary

ApplicationWestern Blot
Sample TestedImmortalised Pteropus alecto kidney cell lysate (PaKiT03)
SpeciesOther
FileView PDF

Blocking

Blocking Details5% Skim milk in tris buffered saline with 5% tween (TBST)

Details

Detection NotesChemiluminescence

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Triosephosphate isomerase Antibody (NBP1-31470) (0)

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Secondary Antibodies

 

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Bioinformatics Tool for Triosephosphate isomerase Antibody (NBP1-31470)

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We at Novus Biologicals are one of the leading antibody suppliers for diabetes research. An aging population, and the increasing incidence of type 2 diabetes, makes it an area of increasing interest - especially as there is often a close link to cance...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
09/21/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol TPI1
Entrez
Uniprot