Triosephosphate isomerase Antibody

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Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Immunodetection of glycolytic enzymes within bat and human cells. Immunodetection of Eno1, Tpi1, Pgam1 and beta2-Tub (load control) in Poly I:C transfected ...read more
Immunocytochemistry/ Immunofluorescence: Triosephosphate isomerase Antibody [NBP1-31470] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase stained by Triosephosphate ...read more
Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NBP1-31470] - Paraffin-embedded SNU-16 xenograft, using antibody at 1:100 dilution.
Genetic Strategies: Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was ...read more
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody diluted by 1:1000. ...read more
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody diluted by 1:1000. ...read more
Western Blot: Triosephosphate isomerase Antibody [NBP1-31470] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [C2C3], C-term ...read more
Mainly CK14-positive, but not CK8-positive, mammary epithelial cells are HIF-2 alpha positive at day 14 post weaning.Left panels 20x and right 40x objective. Size bars 50 μm. a, A, b and B. Double IHC for HIF-2 alpha ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

Triosephosphate isomerase Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.
Predicted Species
Chimpanzee (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPI1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA -Assay dependent
  • Flow Cytometry -Reported in scientific literature (PMID: 22262862)
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31470 in the following applications:

Publications
Read Publications using
NBP1-31470 in the following applications:

  • WB
    4 publications

Reactivity Notes

Bovine reactivity reported in scientific literature (PMID: 29892502).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for Triosephosphate isomerase Antibody

  • EC 5.3.1.1
  • MGC88108
  • TIM
  • TPI
  • triosephosphate isomerase 1
  • triosephosphate isomerase
  • Triose-phosphate isomerase

Background

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Triosephosphate isomerase Antibody (NBP1-31470)(11)

We have publications tested in 2 confirmed species: Bovine, Bt.

We have publications tested in 1 application: WB.


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WB
(4)
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Bovine
(5)
Bt
(2)
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Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using NBP1-31470 Applications Species
Duan Y, Li J, Wang F et al. Protein modifications throughout the lung cancer proteome unravel the cancer-specific regulation of glycolysis Cell reports 2021-12-21 [PMID: 34936872]
Gagaoua M, Bonnet M, Picard B Protein Array-Based Approach to Evaluate Biomarkers of Beef Tenderness and Marbling in Cows: Understanding of the Underlying Mechanisms and Prediction Foods (Basel, Switzerland) 2020-08-26 [PMID: 32858893] (Bovine) Bovine
Bonnet M, Soulat J, Bons J et al. Quantification of biomarkers for beef meat qualities using a combination of Parallel Reaction Monitoring- and antibody-based proteomics Food Chem 2020-02-10 [PMID: 32078991] (WB, Bovine) WB Bovine
Picard B, Gagaoua M, Al Jammas M, Bonnet M et al. Beef tenderness and intramuscular fat proteomic biomarkers: Effect of gender and rearing practices. J Proteomics. 2019-03-17 [PMID: 30894324] (WB, Bovine) WB Bovine
Gagaoua M, Bonnet M, De Koning L et al. Reverse Phase Protein array for the quantification and validation of protein biomarkers of beef qualities: The case of meat color from Charolais breed. Meat Sci 2018-07-05 [PMID: 30015160] (Bovine) Bovine
Picard B, Gagaoua M, Al-Jammas M et al. Beef tenderness and intramuscular fat proteomic biomarkers: muscle type effect. PeerJ 2018-06-07 [PMID: 29892502] (Bovine) Bovine
Gagaoua M, Bonnet M, Ellies-Oury MP et al. Reverse phase protein arrays for the identification/validation of biomarkers of beef texture and their use for early classification of carcasses. Food Chem 2018-01-10 [PMID: 29412918]
Mok L, Wynne JW, Ford K et al. Proteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C. Proteome Sci 2015 Nov 2 [PMID: 26535029] (WB, Bt)

Details:
Pteropus alecto (Black fruit bat)
WB Bt
Mok L, Wynne JW, Ford K et al. Proteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C Proteome Science 2015-12-01 [PMID: 26535029]
Mok L, Wynne JW, Ford K et al. Proteomic analysis of Pteropus alecto kidney cells in response to the viral mimic, Poly I:C Proteome Sci 2015 Nov 2 [PMID: 26535029] (WB, Bt)

Details:
Pteropus alecto (Black fruit bat)
WB Bt
Show All 11 Publications.

Review for Triosephosphate isomerase Antibody (NBP1-31470) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Other.

Reviews using NBP1-31470:
Filter by Applications
WB
(1)
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Other
(1)
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Images Ratings Applications Species Date Details
Western Blot Triosephosphate isomerase NBP1-31470
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5
reviewed by:
Verified Customer
WB Other 09/21/2015
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Summary

ApplicationWestern Blot
Sample TestedImmortalised Pteropus alecto kidney cell lysate (PaKiT03)
SpeciesOther

Product General Protocols

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Secondary Antibodies

 

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Verified Customer
09/21/2015
Application: WB
Species: Other

Bioinformatics

Gene Symbol TPI1
Entrez
Uniprot