Albumin Antibody (188835) [Unconjugated] - Serum

Images

 
Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary ...read more
Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Serum Albumin ...read more
Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room ...read more
Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1 µg/mL for 1 hour at room temperature followed ...read more
Orthogonal Strategies: Serum Albumin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human liver probed with ACD RNAScope® Probe (Catalog # 600941) and stained using ACD ...read more
Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serum ...read more
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, CyTOF-ready, Dual ISH-IHC, Flow, ICC/IF
Clone
188835
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
       

Orthogonal Strategies

 

Order Details

Albumin Antibody (188835) [Unconjugated] - Serum Summary

Immunogen
Human Serum Albumin
Specificity
Detects human Serum Albumin in direct ELISAs and Western blots. Does not detect mouse Serum Albumin in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ALB
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 0.05-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.1-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 1-10 ug/mL
  • Western Blot 0.2-1 ug/mL
Publications
Read Publications using
MAB1455 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Albumin Antibody (188835) [Unconjugated] - Serum

  • ALB
  • Albumin
  • cell growth inhibiting protein 42
  • DKFZp779N1935
  • growth-inhibiting protein 20
  • PRO0883
  • PRO0903
  • PRO1341
  • serum albumin

Background

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Albumin Antibody (MAB1455)(28)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: ELISA Detection, Flow Cytometry, ICC, IHC, IHC-Fr, Western Blot.


Filter By Application
ELISA Detection
(1)
Flow Cytometry
(2)
ICC
(15)
IHC
(3)
IHC-Fr
(3)
Western Blot
(3)
All Applications
Filter By Species
Human
(19)
Mouse
(7)
Rat
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using MAB1455 Applications Species
M Ramirez-Ga, V Berge, A Lin?, A Llorente Potential of miRNAs in urinary extracellular vesicles for management of active surveillance in prostate cancer patients British Journal of Cancer, 2021;0(0):. 2021 [PMID: 34811506] (Western Blot, Human) Western Blot Human
F Michielin, GG Giobbe, C Luni, Q Hu, I Maroni, MR Orford, A Manfredi, L Di Filippo, AL David, D Cacchiarel, P De Coppi, S Eaton, N Elvassore The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs Cell Rep, 2020;33(9):108453. 2020 [PMID: 33264615] (IHC, Human) IHC Human
M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh Fecal host biomarkers predicting severity of Clostridioides difficile infection JCI Insight, 2020;0(0):. 2020 [PMID: 33232301] (Western Blot, Mouse) Western Blot Mouse
J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li Repair of acute liver damage with immune evasive hESC derived hepato-blasts Stem Cell Res, 2020;49(0):102010. 2020 [PMID: 33011360] (ICC, Human) ICC Human
G Sorrentino, S Rezakhani, E Yildiz, S Nuciforo, MH Heim, MP Lutolf, K Schoonjans Mechano-modulatory synthetic niches for liver organoid derivation Nat Commun, 2020;11(1):3416. 2020 [PMID: 32651372] (ICC, Human) ICC Human
N Mori, Y Akagi, Y Imai, Y Takayama, YS Kida Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue Sci Rep, 2020;10(1):5646. 2020 [PMID: 32286353] (IHC, Human) IHC Human
ES Famulari, V Navarro-Ta, MB Herrera Sa, G Bortolussi, M Gai, L Conti, L Silengo, E Tolosano, C Tetta, AF Muro, G Camussi, S Fagoonee, F Altruda Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice Sci Rep, 2020;10(1):887. 2020 [PMID: 31965023] (ICC, Human) ICC Human
F Tonon, GG Giobbe, A Zambon, C Luni, O Gagliano, A Floreani, G Grassi, N Elvassore In vitro metabolic zonation through oxygen gradient on a chip Sci Rep, 2019;9(1):13557. 2019 [PMID: 31537830] (ICC, Human) ICC Human
AC Brook, RH Jenkins, A Clayton, A Kift-Morga, AC Raby, AP Shephard, B Mariotti, SM Cuff, F Bazzoni, T Bowen, DJ Fraser, M Eberl Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis Sci Rep, 2019;9(1):10136. 2019 [PMID: 31300703] (ELISA Detection, Human) ELISA Detection Human
C Gu, P Li, W Liu, Y Zhou, WS Tan The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 30950200] (Western Blot, Human) Western Blot Human
Show All 28 Publications.

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Product General Protocols

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Secondary Antibodies

 

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Blogs on Albumin.

Albumin - a family of globular protein
Albumin is a soluble and globular monomeric protein encoded by chromosome 4 that comprises about half of the protein found in blood serum. It functions as a carrier protein for steroids, fatty acids, and thyroid hormones as well as stabilizing extrace...  Read full blog post.

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Bioinformatics

Gene Symbol ALB