Albumin Antibody (188835) [Unconjugated] - Serum

Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Serum Albumin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human liver probed with ACD RNAScope® Probe (Catalog # 600941) and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.05 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems,Catalog # VC001) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, CyTOF-ready, Dual ISH-IHC, Flow, ICC/IF
• Clone: 188835
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Albumin Antibody (188835) [Unconjugated] - Serum Summary

• Immunogen: Human Serum Albumin
• Specificity: Detects human Serum Albumin in direct ELISAs and Western blots. Does not detect mouse Serum Albumin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 0.05-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.1-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 1-10 ug/mL
  • Western Blot 0.2-1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Albumin Antibody (188835) [Unconjugated] - Serum

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Albumin Antibody (MAB1455)(28)

Publications using MAB1455

• M Ramirez-Ga, V Berge, A Lin?, A Llorente Potential of miRNAs in urinary extracellular vesicles for management of active surveillance in prostate cancer patients British Journal of Cancer, 2021;0(0):. 2021 [PMID: 34811506] (Western Blot, Human)
• F Michielin, GG Giobbe, C Luni, Q Hu, I Maroni, MR Orford, A Manfredi, L Di Filippo, AL David, D Cacchiarel, P De Coppi, S Eaton, N Elvassore The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs Cell Rep, 2020;33(9):108453. 2020 [PMID: 33264615] (IHC, Human)
• M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh Fecal host biomarkers predicting severity of Clostridioides difficile infection JCI Insight, 2020;0(0):. 2020 [PMID: 33232301] (Western Blot, Mouse)
• J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li Repair of acute liver damage with immune evasive hESC derived hepato-blasts Stem Cell Res, 2020;49(0):102010. 2020 [PMID: 33011360] (ICC, Human)
• G Sorrentino, S Rezakhani, E Yildiz, S Nuciforo, MH Heim, MP Lutolf, K Schoonjans Mechano-modulatory synthetic niches for liver organoid derivation Nat Commun, 2020;11(1):3416. 2020 [PMID: 32651372] (ICC, Human)
• N Mori, Y Akagi, Y Imai, Y Takayama, YS Kida Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue Sci Rep, 2020;10(1):5646. 2020 [PMID: 32286353] (IHC, Human)
• ES Famulari, V Navarro-Ta, MB Herrera Sa, G Bortolussi, M Gai, L Conti, L Silengo, E Tolosano, C Tetta, AF Muro, G Camussi, S Fagoonee, F Altruda Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice Sci Rep, 2020;10(1):887. 2020 [PMID: 31965023] (ICC, Human)
• F Tonon, GG Giobbe, A Zambon, C Luni, O Gagliano, A Floreani, G Grassi, N Elvassore In vitro metabolic zonation through oxygen gradient on a chip Sci Rep, 2019;9(1):13557. 2019 [PMID: 31537830] (ICC, Human)
• AC Brook, RH Jenkins, A Clayton, A Kift-Morga, AC Raby, AP Shephard, B Mariotti, SM Cuff, F Bazzoni, T Bowen, DJ Fraser, M Eberl Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis Sci Rep, 2019;9(1):10136. 2019 [PMID: 31300703] (ELISA Detection, Human)
• C Gu, P Li, W Liu, Y Zhou, WS Tan The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium J. Cell. Mol. Med., 2019;0(0):. 2019 [PMID: 30950200] (Western Blot, Human)
• H Okada, C Nakanishi, S Yoshida, M Shimojima, J Yokawa, M Mori, H Tada, T Yoshimuta, K Hayashi, T Yamano, R Hanayama, M Yamagishi, MA Kawashiri Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia Sci Rep, 2019;9(1):4695. 2019 [PMID: 30886174] (ICC, Human)
• A Fernández-, M Ortega-Rib, S Guixé-Munt, J Gracia-San 4 in 1: Antibody-free protocol for isolating the main hepatic cells from healthy and cirrhotic single rat livers J. Cell. Mol. Med., 2018;0(0):. 2018 [PMID: 30417530] (ICC, Rat)
• A Skrzypczyk, S Kehr, I Krystel, SH Bernhart, S Giri, A Bader, PF Stadler Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes Stem Cells Int, 2018;2018(0):5692840. 2018 [PMID: 30210551] (ICC, Human)
• MB Herrera Sa, S Previdi, S Bruno, V Fonsato, MC Deregibus, S Kholia, S Petrillo, E Tolosano, R Critelli, M Spada, R Romagnoli, M Salizzoni, C Tetta, G Camussi Extracellular vesicles from human liver stem cells restore argininosuccinate synthase deficiency Stem Cell Res Ther, 2017;8(1):176. 2017 [PMID: 28750687] (Flow Cytometry, Human)
• CI Real, M Werner, A Paul, G Gerken, JF Schlaak, A Vaillant, R Broering Nucleic acid-based polymers effective against hepatitis B Virus infection in patients don't harbor immunostimulatory properties in primary isolated liver cells Sci Rep, 2017;7(0):43838. 2017 [PMID: 28272460] (ICC, Human)
• A single heterochronic blood exchange reveals rapid inhibition of multiple tissues by old blood Nat Commun, 2016;7(0):13363. 2016 [PMID: 27874859] (IHC-Fr, Mouse)
• Effect of Secreted Molecules of Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells on Acute Hepatic Failure Model Stem Cells Dev, 2016;0(0):. 2016 [PMID: 27676103] (IHC-Fr, Mouse)
• Park H, Choi Y, Kim J, Chun H, Im I, Yoon S, Han Y, Song C, Kim H Differences in the Epigenetic Regulation of Cytochrome P450 Genes between Human Embryonic Stem Cell-Derived Hepatocytes and Primary Hepatocytes. PLoS ONE, 2015;10(7):e0132992. 2015 [PMID: 26177506] (Flow Cytometry, Human)
• Elmes M, Kaczocha M, Berger W, Leung K, Ralph B, Wang L, Sweeney J, Miyauchi J, Tsirka S, Ojima I, Deutsch D Fatty acid-binding proteins (FABPs) are intracellular carriers for Delta9-tetrahydrocannabinol (THC) and cannabidiol (CBD). J Biol Chem, 2015;290(14):8711-21. 2015 [PMID: 25666611] (IHC, Mouse)
• Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M Identification of transcription factors for lineage-specific ESC differentiation. Stem Cell Reports, 2013;1(6):545-59. 2013 [PMID: 24371809]
• Hannan , Nicholas, Segeritz , Charis-P, Touboul , Thomas, Vallier , Ludovic Production of hepatocyte-like cells from human pluripotent stem cells. Nat Protoc, 2013;8(2):430-7. 2013 [PMID: 23370178] (ICC, Human)
• Byun K, Bayarsaikhan E, Kim D, Kim CY, Mook-Jung I, Paek SH, Kim SU, Yamamoto T, Won MH, Song BJ, Park YM, Lee B Induction of neuronal death by microglial AGE-albumin: implications for Alzheimer's disease. PLoS ONE, 2012;7(5):e37917. 2012 [PMID: 22662249] (ICC, Human)
• Hoang S, Liauw J, Choi M Netrin-4 enhances angiogenesis and neurologic outcome after cerebral ischemia. J. Cereb. Blood Flow Metab., 2009;29(2):385-97. 2009 [PMID: 18985053] (IHC-Fr, Mouse)
• Yang L, Jung Y, Omenetti A, Witek RP, Choi S, Vandongen HM, Huang J, Alpini GD, Diehl AM Fate-mapping evidence that hepatic stellate cells are epithelial progenitors in adult mouse livers. Stem Cells, 2008;26(8):2104-13. 2008 [PMID: 18511600] (ICC, Mouse)
• Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition. J. Biol. Chem., 2007;282(32):23337-47. 2007 [PMID: 17562716] (ICC, Mouse)
• Ilancheran S, Michalska A, Peh G, Wallace EM, Pera M, Manuelpillai U Stem cells derived from human fetal membranes display multilineage differentiation potential. Biol. Reprod., 2007;77(3):577-88. 2007 [PMID: 17494917] (ICC, Human)
• Herrera MB, Bruno S, Buttiglieri S, Tetta C, Gatti S, Deregibus MC, Bussolati B, Camussi G Isolation and characterization of a stem cell population from adult human liver. Stem Cells, 2006;24(12):2840-50. 2006 [PMID: 16945998] (ICC, Human)
• Baharvand H, Hashemi SM, Kazemi Ashtiani S, Farrokhi A Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro. Int. J. Dev. Biol., 2006;50(7):645-52. 2006 [PMID: 16892178] (ICC, Human)

Bioinformatics

• Gene Symbol: ALB