Albumin Antibody (188835) [Unconjugated] - Serum

Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Serum Albumin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human liver probed with ACD RNAScope® Probe (Catalog # 600941) and stained using ACD RNAscope® 2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.05 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems,Catalog # VC001) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue).

Heterochronic blood exchange effects on muscle regeneration and performance.One day after blood exchange mice were injured by intramuscular injections of CTX into TA. Five days after injury, TA muscles were isolated, cryo-sectioned and analysed. (a) TA muscles from young mice receiving young blood (YY), young mice receiving old blood (YO), old mice receiving young blood (OY) and old mice receiving old blood (OO) were analysed by haematoxylin and eosin (H&E) staining and immunofluorescence with anti-eMyHC antibody. Representative images show an injury site and nascent de-novo formed eMyHC+ myofibers which are smaller in size with central nuclei than uninjured myofibers. Scale bar, 50 μm for H&E panel and 25 μm for immunofluorescence panel. (b,c) Regeneration indices ±s.e.m. were quantified from H&E images (b) and eMyHC images (c) by counting the number of nascent de-novo formed myofibers and dividing by the total number of nuclei present at the injury/regeneration site. By H&E: *P<0.05 N=4 per group. Significant students t test differences exist between YO and OY (P=0.045), YY and OY (P=0.043), YY and OO (P=0.0004), YO and OO (P=0.0042) and between OY and OO (P=0.015). By eMyHC: *P<0.05, N=4 per group; OY and OO P=0.041, YY and OO P=0.00009, and YO to OO P=0.001. (d) Fibrotic/inflammatory indexes were quantified as total injury area minus regenerated myofiber area, per injury site, using the H&E images15. T-test **P<0.005, n=3–4 per group. Muscle from old to old isochronic exchange had diminished regenerative capacity and more fibrosis, as compared with muscle from young to young isochronic exchange. Heterochronic blood exchange significantly improved regeneration of old muscle after experimental injury and reduced fibrosis, but no significant decline in young muscle regeneration was seen. (e) A four-limb hanging test was conducted with isochronically and heterochronically transfused mice that were not injured, before and at 6 days after the blood exchange. Maximal hanging time was multiplied by body weight (hang index). T-test n=4–8, P=0.01 YY post transfusion compared with O training, and YO, OY, OO post-transfusion performance. Y to O training and YO, OY and OO were NS=not statistically different. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27874859), licensed under a CC-BY license. Not internally tested by R&D Systems.

Heterochronic blood exchange effects on hepatogenesis and liver fibrosis and adiposity.(a) Livers from YY, YO, OY and OO mice with and without experimental muscle injury as above were cryo-sectioned at 10 μm and immuno-stained for Ki67 (red), hepatocyte marker albumin (green) and Hoechst (blue). Representative images show YY livers with and without injury. Scale bar, 50 μm. (B&C. Quantification of hepatocyte proliferation was by counting the average number of Ki67+,abumin+,Hoechst+ cells per 10 μm section from multiple sections of each blood exchange cohort. (b) Old hepatocyte showed increased proliferation and young hepatocytes showed less proliferation with heterochronic blood as compared with isochronic blood exchanges in animals with injured muscle (t test P=0.00028). (c) This trend continues without muscle injury, but the total numbers of proliferating hepatocytes decline by twofold, (P=0.02411). *P<0.05; **P<0.005; n=3–5. (d) As previously published4, there were fibrotic clusters exclusively in the old livers of small Ki67+ve, albumin negative Ki67+ cells. Scale bar, 50 μm, × 40 magnification. (e,f) Fibrotic index was calculated as the average number of albumin negative proliferative cell clusters per four 10 μm sections. The fibrotic index diminished in old mice exchanged with young blood with muscle injury (e) (t test P=0.048 N=4, *P<0.05) or without (f) (t test P=0.00776. N=3; **P<0.005). (g) Liver adiposity was assayed by Oil Red in 10 μm cryosections. Shown are representative images acquired at × 20 magnification. (h). Liver adiposity (red) was quantified by Image J, dramatically increased with age and was attenuated by young blood in old mice (t test N=3, P=0.022), while adiposity remained unchanged in young mice that were transfused with the old blood (see Supplementary Figure 4). Shown are means±s.e.m. for all histograms. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27874859), licensed under a CC-BY license. Not internally tested by R&D Systems.

Heterochronic blood exchange effects on hepatogenesis and liver fibrosis and adiposity.(a) Livers from YY, YO, OY and OO mice with and without experimental muscle injury as above were cryo-sectioned at 10 μm and immuno-stained for Ki67 (red), hepatocyte marker albumin (green) and Hoechst (blue). Representative images show YY livers with and without injury. Scale bar, 50 μm. (B&C. Quantification of hepatocyte proliferation was by counting the average number of Ki67+,abumin+,Hoechst+ cells per 10 μm section from multiple sections of each blood exchange cohort. (b) Old hepatocyte showed increased proliferation and young hepatocytes showed less proliferation with heterochronic blood as compared with isochronic blood exchanges in animals with injured muscle (t test P=0.00028). (c) This trend continues without muscle injury, but the total numbers of proliferating hepatocytes decline by twofold, (P=0.02411). *P<0.05; **P<0.005; n=3–5. (d) As previously published4, there were fibrotic clusters exclusively in the old livers of small Ki67+ve, albumin negative Ki67+ cells. Scale bar, 50 μm, × 40 magnification. (e,f) Fibrotic index was calculated as the average number of albumin negative proliferative cell clusters per four 10 μm sections. The fibrotic index diminished in old mice exchanged with young blood with muscle injury (e) (t test P=0.048 N=4, *P<0.05) or without (f) (t test P=0.00776. N=3; **P<0.005). (g) Liver adiposity was assayed by Oil Red in 10 μm cryosections. Shown are representative images acquired at × 20 magnification. (h). Liver adiposity (red) was quantified by Image J, dramatically increased with age and was attenuated by young blood in old mice (t test N=3, P=0.022), while adiposity remained unchanged in young mice that were transfused with the old blood (see Supplementary Figure 4). Shown are means±s.e.m. for all histograms. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27874859), licensed under a CC-BY license. Not internally tested by R&D Systems.

Differentiation of hESCs into HLCs.a The relative hepatocyte (ALB, AAT, CK18, and ASGPR1), cholangiocyte (SOX9), and hepatoblast (AFP) gene expression levels of day 19 differentiated cells with different treatments (groups A and B) were determined by qPCR. HGF (hepatocyte growth factor, 20 ng/mL); OSM (oncostatin M, 20 ng/mL); Dex (dexamethasone, 10 µM); SB431542 (TGF beta inhibitor, 2 µM); and RO4929097 (Notch inhibitor, 1 µM). b Immunofluorescence analysis of ALB, AAT, ASGPR1, and CK18 expression in group B-induced differentiated cells on day 19. c The expression levels of ALB and ASGPR1 in group B-induced differentiated cells were determined by flow cytometry on day 19. Isotype control antibodies were used as controls. d The relative hepatocyte (ALB, AAT, CK18, ASGPR1, and AFP) gene expression levels of differentiated HLCs (group B) compared with those of hESCs and primary human hepatocytes (PHHs) were determined by qPCR. e Albumin secretion of HLCs (black line) on days 12, 14, 16, 18, and 20 and PHHs (dotted line) on day 2 were determined by ELISA. *p < 0.05, **p < 0.01; data are represented as the mean ± SD. Scale bar, 50 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31601782), licensed under a CC-BY license. Not internally tested by R&D Systems.

NLRP3 inflammasome-dependent pyroptosis occurs in liver fibrosis. (A) IHC staining for GSDMD, IL-1 beta , and IL-18 in liver sections from liver fibrosis patients and HCs. Scale bar: 40 µm. (B–D) ELISA analyses of serum levels of GSDMD (B), IL-1 beta (C), and IL-18 (D) in liver fibrosis patients (n = 89) and HCs (n = 60). (E) Representative immunofluorescence images of NLRP3 (red) and albumin (hepatocyte marker) (top), F4/80 (KC marker) (middle) or alpha -SMA (HSC marker) (bottom) (green) from the human fibrotic liver tissues. Scale bar: 40 µm. (F) Schematic diagram of the study. Liver fibrosis was induced by CCl4 injection for 8 weeks. (G) Representative mouse liver histology of H&E, Sirius Red staining, and IHC staining for alpha -SMA, GSDMD, and IL-1 beta . Black scale bar: 100 µm; Red scale bar: 50 µm. (H–J) ELISA analyses for serum levels of GSDMD (H), IL-1 beta (I), and IL-18 (J) in CCl4 group mouse (n = 5) and vehicle group mouse (n = 5). (K) Representative immunofluorescence images of NLRP3 (red) and albumin (hepatocyte marker) (top), F4/80 (KC marker) (middle) or alpha -SMA (HSC marker) (bottom) (green) from the 8-week CCl4-treated mouse liver. The vehicle group mouse liver was used as a control. Scale bar: 40 µm. (L) The qRT-PCR analysis for mRNA levels of IL-1 beta in THP-1 macrophages treated with LPS to induce pyroptosis. (M) ELISA analysis for IL-1 beta expression in supernatants from THP-1. (N) Western blot analysis of COL1A1, alpha -SMA, and TGF-beta expression in LX-2 cells which were exposed to CM from LPS-treated THP-1 macrophages. The protein expression was quantified by densitometry and normalized to beta -actin and are shown as fold changes relative to the control group (right panel). ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36429008), licensed under a CC-BY license. Not internally tested by R&D Systems.

NLRP3 inflammasome-dependent pyroptosis occurs in liver fibrosis. (A) IHC staining for GSDMD, IL-1 beta , and IL-18 in liver sections from liver fibrosis patients and HCs. Scale bar: 40 µm. (B–D) ELISA analyses of serum levels of GSDMD (B), IL-1 beta (C), and IL-18 (D) in liver fibrosis patients (n = 89) and HCs (n = 60). (E) Representative immunofluorescence images of NLRP3 (red) and albumin (hepatocyte marker) (top), F4/80 (KC marker) (middle) or alpha -SMA (HSC marker) (bottom) (green) from the human fibrotic liver tissues. Scale bar: 40 µm. (F) Schematic diagram of the study. Liver fibrosis was induced by CCl4 injection for 8 weeks. (G) Representative mouse liver histology of H&E, Sirius Red staining, and IHC staining for alpha -SMA, GSDMD, and IL-1 beta . Black scale bar: 100 µm; Red scale bar: 50 µm. (H–J) ELISA analyses for serum levels of GSDMD (H), IL-1 beta (I), and IL-18 (J) in CCl4 group mouse (n = 5) and vehicle group mouse (n = 5). (K) Representative immunofluorescence images of NLRP3 (red) and albumin (hepatocyte marker) (top), F4/80 (KC marker) (middle) or alpha -SMA (HSC marker) (bottom) (green) from the 8-week CCl4-treated mouse liver. The vehicle group mouse liver was used as a control. Scale bar: 40 µm. (L) The qRT-PCR analysis for mRNA levels of IL-1 beta in THP-1 macrophages treated with LPS to induce pyroptosis. (M) ELISA analysis for IL-1 beta expression in supernatants from THP-1. (N) Western blot analysis of COL1A1, alpha -SMA, and TGF-beta expression in LX-2 cells which were exposed to CM from LPS-treated THP-1 macrophages. The protein expression was quantified by densitometry and normalized to beta -actin and are shown as fold changes relative to the control group (right panel). ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36429008), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, CyTOF-ready, Dual ISH-IHC, Flow, ICC/IF
• Clone: 188835
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Albumin Antibody (188835) [Unconjugated] - Serum Summary

• Immunogen: Human Serum Albumin
• Specificity: Detects human Serum Albumin in direct ELISAs and Western blots. Does not detect mouse Serum Albumin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ALB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Dual RNAscope ISH-IHC 0.05-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 0.1-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells
  • Simple Western 1-10 ug/mL
  • Western Blot 0.2-1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Albumin Antibody (188835) [Unconjugated] - Serum

• ALB
• Albumin
• cell growth inhibiting protein 42
• DKFZp779N1935
• growth-inhibiting protein 20
• PRO0883
• PRO0903
• PRO1341
• serum albumin

Background

Albumins are a family of globular proteins, the most common of which are serum albumins. Albumins are commonly found in blood plasma, and are unique from other blood proteins in that they are not glycosylated. Albumin is a 65-70 kDa protein with serum albumin being the main protein of human blood plasma. It binds water, cations (such as Ca2+, Na+ and K+), fatty acids, hormones, bilirubin, thyroxine (T4) and pharmaceuticals (including barbiturates) - its main function is to regulate the colloidal osmotic pressure of blood. Albumin comprises three homologous domains that assemble to form a heart-shaped molecule. Each domain is a product of two subdomains that possess common structural motifs. The principal regions of ligand binding to human serum albumin are located in hydrophobic cavities in subdomains IIA and IIIA, which exhibit similar chemistry. Structurally, the serum albumins are similar, each domain containing five or six internal disulfide bonds.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Albumin Antibody (MAB1455)(57)

Publications using MAB1455

• Guo, XL;Wang, YD;Liu, YJ;Chu, L;Zhu, H;Hu, Y;Wu, RY;Xie, HY;Yu, J;Li, SP;Xiong, ZY;Li, RY;Ke, F;Chen, L;Chen, GQ;Chen, L;Bai, F;Enver, T;Li, GH;Li, HF;Hong, DL; Fetal hepatocytes protect the HSPC genome via fetuin-A Nature 2024-12-04 [PMID: 39633051] (Immunohistochemistry, Human)
• Y Chen, L Chen, X Wu, Y Zhao, Y Wang, D Jiang, X Liu, T Zhou, S Li, Y Wei, Y Liu, C Hu, B Zhou, J Qin, H Ying, Q Ding Acute liver steatosis translationally controls the epigenetic regulator MIER1 to promote liver regeneration in a study with male mice Nature Communications, 2023-03-18;14(1):1521. 3/18/2023 [PMID: 36934083]
• Xiaoshan Wu, Dacheng Jiang, Yi Yang, Shuang Li, Qiurong Ding Modeling drug-induced liver injury and screening for anti-hepatofibrotic compounds using human PSC-derived organoids Cell Regeneration 3/3/2023 [PMID: 36864321]
• S Ghafoory, C Stengl, S Kopany, M Mayadag, N Mechtel, B Murphy, S Schattschn, N Wilhelmi, S Wölfl Oxygen Gradient Induced in Microfluidic Chips Can Be Used as a Model for Liver Zonation Cells, 2022-11-23;11(23):. 2022-11-23 [PMID: 36496994] (ICC/IF, Human)
• Rong Li, Yang Zhao, Jeffrey J. Yourick, Robert L. Sprando, Xiugong Gao Phenotypical, functional and transcriptomic comparison of two modified methods of hepatocyte differentiation from human induced pluripotent stem cells Biomedical Reports 3/23/2022 [PMID: 35371477]
• Satoshi Muraoka, Masayo Hirano, Junko Isoyama, Satoshi Nagayama, Takeshi Tomonaga, Jun Adachi Comprehensive proteomic profiling of plasma and serum phosphatidylserine-positive extracellular vesicles reveals tissue-specific proteins iScience 4/15/2022 [PMID: 35340435]
• MAS de Toledo, X Fu, F Kluge, K Götz, S Schmitz, P Wanek, HM Schüler, K Pannen, N Chatain, S Koschmiede, TH Brümmendor, M Zenke CRISPR/Cas9-engineered human ES cells harboring heterozygous and homozygous c-KIT knockout Stem Cell Research, 2022-03-01;60(0):102732. 2022-03-01 [PMID: 35279545] (ICC, Human)
• M Ramirez-Ga, V Berge, A Lin?, A Llorente Potential of miRNAs in urinary extracellular vesicles for management of active surveillance in prostate cancer patients British Journal of Cancer, 2021-11-22;0(0):. 2021-11-22 [PMID: 34811506] (Western Blot, Human)
• Alex P. Shephard, Peter Giles, Mariama Mbengue, Amr Alraies, Lisa K. Spary, Howard Kynaston et al. Stroma‐derived extracellular vesicle mRNA signatures inform histological nature of prostate cancer Journal of Extracellular Vesicles 10/1/2021 [PMID: 34596356]
• Baoyan Xu, Qiao Yang, Yingzi Tang, Zhaoxia Tan, Haiyan Fu, Jing Peng et al. SIX1/EYA1 are novel liver damage biomarkers in chronic hepatitis B and other liver diseases Annals of Translational Medicine 6/1/2021 [PMID: 34277792]
• Megan I. Mitchell, Iddo Z. Ben‐Dov, Christina Liu, Kenny Ye, Kar Chow, Yael Kramer et al. Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV‐CATCHER): A customizable purification assay designed for small‐RNA biomarker identification and evaluation of circulating small‐EVs Journal of Extracellular Vesicles 6/3/2021 [PMID: 34122779]
• F Michielin, GG Giobbe, C Luni, Q Hu, I Maroni, MR Orford, A Manfredi, L Di Filippo, AL David, D Cacchiarel, P De Coppi, S Eaton, N Elvassore The Microfluidic Environment Reveals a Hidden Role of Self-Organizing Extracellular Matrix in Hepatic Commitment and Organoid Formation of hiPSCs Cell Rep, 2020-12-01;33(9):108453. 2020-12-01 [PMID: 33264615] (ICC, IHC, Human)
• M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh Fecal host biomarkers predicting severity of Clostridioides difficile infection JCI Insight, 2021-01-11;0(0):. 2021-01-11 [PMID: 33232301] (Western Blot, Human)
• Cao J, O'Day DR, Pliner HA et al. A human cell atlas of fetal gene expression Science 11/13/2020 [PMID: 33184181]
• J Liu, T Pan, Y Chen, Y Liu, F Yang, Q Chen, N Abbas, M Zhong, Q Zhang, Y Xu, YX Li Repair of acute liver damage with immune evasive hESC derived hepato-blasts Stem Cell Res, 2020-09-26;49(0):102010. 2020-09-26 [PMID: 33011360] (ICC, IHC, Human, Mouse)
• Stefan D. Gentile, Andreas P. Kourouklis, Hyeon Ryoo, Gregory H. Underhill Integration of Hydrogel Microparticles With Three-Dimensional Liver Progenitor Cell Spheroids Frontiers in Bioengineering and Biotechnology 2020-07-21 [PMID: 32793571] (Immunocytochemistry, Mouse)
• G Sorrentino, S Rezakhani, E Yildiz, S Nuciforo, MH Heim, MP Lutolf, K Schoonjans Mechano-modulatory synthetic niches for liver organoid derivation Nat Commun, 2020-07-10;11(1):3416. 2020-07-10 [PMID: 32651372] (ICC, Human)
• PA Subramani, N Vartak-Sha, S Sreekumar, P Mathur, B Nayer, S Dakhore, SK Basavanna, DM Kalappa, RV Krishnamur, B Mukhi, P Mishra, N Yoshida, SK Ghosh, R Shandil, S Narayanan, B Campo, K Hasegawa, AR Anvikar, N Valecha, V Sundaramur Plasmodium vivax liver stage assay platforms using Indian clinical isolates Malar. J., 2020-06-22;19(1):214. 2020-06-22 [PMID: 32571333] (ICC, Human)
• M Mehdipour, C Skinner, N Wong, M Lieb, C Liu, J Etienne, C Kato, D Kiprov, MJ Conboy, IM Conboy Rejuvenation of three germ layers tissues by exchanging old blood plasma with saline-albumin Aging (Albany NY), 2020-05-30;12(10):8790-8819. 2020-05-30 [PMID: 32474458] (IHC, Western Blot, Human, Mouse)
• N Mori, Y Akagi, Y Imai, Y Takayama, YS Kida Fabrication of Perfusable Vascular Channels and Capillaries in 3D Liver-like Tissue Sci Rep, 2020-04-14;10(1):5646. 2020-04-14 [PMID: 32286353] (IHC, Human)
• Musante L, Bontha Sv, La Salvia S et Al. Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs Sci Rep 2/28/2020 [PMID: 32111925]
  
  Details:
  Antibody was used in Western Blot analysis to verify that subcellular contaminants were not interfering with detecting target bio-markers.
• Cao-Sang Truong, Eunhui Seo, Hee-Sook Jun Psoralea corylifolia L. Seed Extract Attenuates Methylglyoxal-Induced Insulin Resistance by Inhibition of Advanced Glycation End Product Formation Oxidative Medicine and Cellular Longevity 12/30/2019 [PMID: 31976027]
• Cao-Sang Truong, Eunhui Seo, Hee-Sook Jun Psoralea corylifolia L. Seed Extract Attenuates Methylglyoxal-Induced Insulin Resistance by Inhibition of Advanced Glycation End Product Formation Oxidative Medicine and Cellular Longevity 2019-12-30 [PMID: 31976027] (Western Blot, Mouse)
• ES Famulari, V Navarro-Ta, MB Herrera Sa, G Bortolussi, M Gai, L Conti, L Silengo, E Tolosano, C Tetta, AF Muro, G Camussi, S Fagoonee, F Altruda Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice Sci Rep, 2020-01-21;10(1):887. 2020-01-21 [PMID: 31965023] (ICC, Human)
• Kang Wang, Fuhua Xu, Shawn P. Campbell, Kyle D. Hart, Tyler Durham, James Maylie et al. Rapid actions of anti‐Müllerian hormone in regulating synaptic transmission and long‐term synaptic plasticity in the hippocampus The FASEB Journal 1/1/2020 [PMID: 31914642]
• C Lipps, P Northe, R Figueiredo, M Rohde, A Brahmer, EM Krämer-Alb, C Liebetrau, CB Wiedenroth, E Mayer, SD Kriechbaum, O Dörr, H Nef, CW Hamm, T Keller, C Troidl Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA Biomolecules, 2019-10-29;9(11):. 10/29/2019 [PMID: 31671920]
• F Tonon, GG Giobbe, A Zambon, C Luni, O Gagliano, A Floreani, G Grassi, N Elvassore In vitro metabolic zonation through oxygen gradient on a chip Sci Rep, 2019-09-19;9(1):13557. 2019-09-19 [PMID: 31537830] (ICC, Human)
• Melod Mehdipour, Jessy Etienne, Chia-Chien Chen, Ranveer Gathwala, Maryam Rehman, Cameron Kato et al. Rejuvenation of brain, liver and muscle by simultaneous pharmacological modulation of two signaling determinants, that change in opposite directions with age Aging (Albany NY) 8/15/2019 [PMID: 31422380]
• AC Brook, RH Jenkins, A Clayton, A Kift-Morga, AC Raby, AP Shephard, B Mariotti, SM Cuff, F Bazzoni, T Bowen, DJ Fraser, M Eberl Neutrophil-derived miR-223 as local biomarker of bacterial peritonitis Sci Rep, 2019-07-12;9(1):10136. 2019-07-12 [PMID: 31300703] (ELISA Detection, Human)
• C Gu, P Li, W Liu, Y Zhou, WS Tan The role of insulin in transdifferentiated hepatocyte proliferation and function in serum-free medium J. Cell. Mol. Med., 2019-04-04;0(0):. 2019-04-04 [PMID: 30950200] (Western Blot, Human)
• H Okada, C Nakanishi, S Yoshida, M Shimojima, J Yokawa, M Mori, H Tada, T Yoshimuta, K Hayashi, T Yamano, R Hanayama, M Yamagishi, MA Kawashiri Function and Immunogenicity of Gene-corrected iPSC-derived Hepatocyte-Like Cells in Restoring Low Density Lipoprotein Uptake in Homozygous Familial Hypercholesterolemia Sci Rep, 2019-03-18;9(1):4695. 2019-03-18 [PMID: 30886174] (ICC, Human)
• Marcus Grohmann, Florian Wiede, Garron T. Dodd, Esteban N. Gurzov, Geraldine J. Ooi, Tariq Butt et al. Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC Cell 11/15/2018 [PMID: 30454647]
• A Fernández-, M Ortega-Rib, S Guixé-Munt, J Gracia-San 4 in 1: Antibody-free protocol for isolating the main hepatic cells from healthy and cirrhotic single rat livers J. Cell. Mol. Med., 2018-11-12;0(0):. 2018-11-12 [PMID: 30417530] (ICC, Rat)
• A Skrzypczyk, S Kehr, I Krystel, SH Bernhart, S Giri, A Bader, PF Stadler Noncoding RNA Transcripts during Differentiation of Induced Pluripotent Stem Cells into Hepatocytes Stem Cells Int, 2018-08-19;2018(0):5692840. 2018-08-19 [PMID: 30210551] (ICC, Human)
• 何琼, Qiong He, 王惠荟, 程涛, 袁卫平, 马钰波 et al. Genetic Correction and Hepatic Differentiation of Hemophilia B-specific Human Induced Pluripotent Stem Cells Chinese Medical Sciences Journal 9/27/2017 [PMID: 28956740]
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Reviews for Albumin Antibody (MAB1455) (3)

Average Rating: 4

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Mouse, Pig.

reviewed by: Verified Customer ICC Pig 12/05/2016

Summary

• Application: Immunocytochemistry
• Sample Tested: pig trabecular meshwork cells
• Species: Pig

reviewed by: Verified Customer Flow Mouse 02/10/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 23072626
• Species: Mouse

reviewed by: Verified Customer Flow Human 02/10/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID 22829291
• Species: Human

Bioinformatics

• Gene Symbol: ALB