Western Blot: Actin Gamma 1 Antibody [NB600-533] - Actin Antibody [NB600-533]-Detection of actin in 3T3 (20 ug) lysates. ECL detection 30 seconds. Lane 1 - 1:5,000 dilution Lane 2 - 1:10,000 dilution Lane 3 - ...read more
Immunocytochemistry/ Immunofluorescence: Actin Gamma 1 Antibody [NB600-533] - Detection of Actin Gamma 1 (Green) in Hela cells using NB600-533 at a 1:50 dilution. Nuclei (Blue) were counterstained using Hoechst 33258.
Western Blot: Actin Gamma 1 Antibody [NB600-533] - Whole cell lysate from HeLa (1, 2.5, 5, 15 and 50 ug) and mouse NIH3T3 cells (50 ug), probed with diluted at 0.04 ug/ml.
Simple Western: Actin Gamma 1 Antibody [NB600-533] - Simple Western lane view shows a specific band for Actin Gamma 1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
The epitope recognized by this antibody maps to the N-terminus of human Actin Gamma 1 [UniProt# P63261]. The N-terminus of Actin Gamma 1 is highly conserved with Beta Actin and preliminary indications are that NB600-533 also recognizes Beta Actin (GeneID 60).
Immunogen affinity purified
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This Actin Gamma 1 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunoprecipitation and Western Blot. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.