Jurkat Whole Cell Lysate


Western Blot: Jurkat Whole Cell Lysate [NB800-PC2] - Western blot analysis of Actin (NB600-532) using Jurkat whole cell lysate using NB800-PC2.
SDS-Page: Jurkat Whole Cell Lysate [NB800-PC2] - Ponceau S staining of Jurkat whole cell lysate using NB800-PC2.

Product Details

Reactivity HuSpecies Glossary
Applications WB, PAGE
1.0 mg/ml

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Jurkat Whole Cell Lysate Summary

Jurkat whole cell lysate (Positive control)


  • SDS-Page
  • Western Blot reported in scientific literature
Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.
Read Publication using NB800-PC2.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
No Preservative
1.0 mg/ml

Lysate Details for Array

Blood Lymph
Subcellular Fraction


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Jurkat Lysate (NB800-PC2)(1)

We have publications tested in 1 application: WB.

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Video Protocols

WB Video Protocol

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Array NB800-PC2

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