Jurkat Whole Cell Lysate

Images

 
Western Blot: Jurkat Whole Cell Lysate [NB800-PC2] - Western blot analysis of Actin (NB600-532) using Jurkat whole cell lysate using NB800-PC2.
SDS-Page: Jurkat Whole Cell Lysate [NB800-PC2] - Ponceau S staining of Jurkat whole cell lysate using NB800-PC2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PAGE
Concentration
1.0 mg/ml

Order Details

Jurkat Whole Cell Lysate Summary

Specificity
Jurkat whole cell lysate (Positive control)

Applications/Dilutions

Application Notes
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane. Western Blot was reported in scientific literature.
Publications
Read Publication using NB800-PC2.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors.
Preservative
No Preservative
Concentration
1.0 mg/ml

Lysate Details for Array

Type
Cell
Tissue
Blood Lymph
Subcellular Fraction
Whole

Notes

Sample Buffer without denaturing agents. Please add 2-mercaptoethanol to 5% v/v before use.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Jurkat Lysate (NB800-PC2)(1)

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

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Additional Array Products

Array NB800-PC2

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Calculators

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