FoxP3 Antibody

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - FFPE tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.

Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.

Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - FFPE tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary antibody and DAB reagent, and nuclei of cells were counterstained with hematoxylin. This FoxP3 antibody generated an expected and a specific nuclear staining of FOXP3 in a subset of cells which are potentially the Treg cells.

Flow Cytometry: FoxP3 Antibody [NB100-39002] - Transfectants were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of positively stained cells are shown.

Immunocytochemistry/Immunofluorescence: FoxP3 Antibody [NB100-39002] - Transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. Representative images (x100) are shown.

Western Blot: FoxP3 Antibody [NB100-39002] - Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and analyzed by Western blot with anti-Foxp3 antiserum (1:2000 dilution).

Western Blot: FoxP3 Antibody [NB100-39002] - Detection of immunoprecipitated FOXP3 from HA-tagged FOXP3 transfected Jurkat T antigen cells.

Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on SK-MEL-28 cells with FoxP3 Antibody NB100-39002 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IB, ICC/IF, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

FoxP3 Antibody Summary

• Immunogen: A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FOXP3
• Purity: Epitope affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
  • Flow Cytometry 1:8000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200 - 1:400
  • Immunohistochemistry-Paraffin 1:200 - 1:400
  • Immunoprecipitation
• Application Notes: Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in immunoblotting reported in scientific literature (PMID: 26485285).

Reactivity Notes

This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Epitope affinity purified

Alternate Names for FoxP3 Antibody

• AIID
• AIIDMGC141961
• anti-human foxp3
• anti-mouse foxp3
• DIETER
• forkhead box P3
• Forkhead Box Protein P3
• foxp3 ihc
• foxp3 paraffin
• FoxP3
• FOXP3delta7
• immune dysregulation, polyendocrinopathy, enteropathy, X-linked
• Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
• IPEX
• JM2
• MGC141961
• MGC141963
• PIDX
• PIDXMGC141963
• SCURFIN
• XPID
• XPIDpolyendocrinopathy, enteropathy, X-linked

Background

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxP3 Antibody (NB100-39002)(13)

Publications using NB100-39002

• Wang Z, Sun K, Xiao Y et al. Niraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models Sci Rep Feb 12 2019 [PMID: 30755715] (IHC-P, Human)
• Yu JW, Bhattacharya S, Yanamandra N et al. Tumor-immune profiling of murine syngeneic tumor models as a framework to guide mechanistic studies and predict therapy response in distinct tumor microenvironments. PLoS ONE. Nov 2 2018 [PMID: 30388137] (IHC-P, Mouse)
• Jiang Y, Cao Z, Ma H et al. Auricular Vagus Nerve Stimulation Exerts Antiinflammatory Effects and Immune Regulatory Function in a 6-OHDA Model of Parkinson's Disease. Neurochem. Res. Nov 1 2018 [PMID: 30311182] (ICC/IF, Rat)
• Schmidt T, Solimani F, Pollmann R et al. Th1/Th17 cell recognition of desmoglein 3 and bullous pemphigoid antigen 180 in lichen planus J. Allergy Clin. Immunol. 2018 Apr 04 [PMID: 29626572] (IHC-P, Human)
• Petrizzo A, Mauriello A, Luciano A, Rea D. Inhibition of tumor growth by cancer vaccine combined with metronomic chemotherapy and anti-PD-1 in a pre-clinical setting. Oncotarget. 2018 Jan 09 [PMID: 29423067] (IHC-P, Mouse)
• Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (Flow, Mouse)
  
  Details:
  This citation used the PE form of this antbody
• Sun J, Lian M, Ma H et al. Competing endogenous RNA network analysis of CD274, IL-10 and FOXP3 co-expression in laryngeal squamous cell carcinoma. Mol Med Rep 2017 Dec 19 [PMID: 29257349] (IHC)
• Busch C, Annamalai B, Abdusalamova K et al. Anaphylatoxins Activate Ca(2+), Akt/PI3-Kinase, and FOXO1/FoxP3 in the Retinal Pigment Epithelium. Front Immunol. 2017 Jun 30 [PMID: 28663750] (ICC/IF, Rat)
• Varga MG, Piazuelo MB, Romero-Gallo J et al. TLR9 activation suppresses inflammation in response to Helicobacter pylori infection. Am. J. Physiol. Gastrointest. Liver Physiol. Nov 1 2016 [PMID: 27758771] (IHC, Rat)
• Zhang MZ, Yao B, Wang Y et al. Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension. J Clin Invest. [PMID: 26485285]
• Kreiter S, Vormehr M, van de Roemer N et al. Mutant MHC class II epitopes drive therapeutic immune responses to cancer Nature 2015 Apr 30 [PMID: 25901682] (IHC-P, Human)
• Rajnai H, Heyning FH, Koens L et al. The density of CD8+ T-cell infiltration and expression of BCL2 predicts outcome of primary diffuse large B-cell lymphoma of bone. Virchows Arch 2013 Dec 4 [PMID: 24306958] (IHC-P, Human)
• Grant BW, Jung SH, Johnson JL et al. A phase 2 trial of extended induction epratuzumab and rituximab for previously untreated follicular lymphoma: CALGB 50701. Cancer 2013 Aug 6 [PMID: 23922187] (IHC, Human)

Review for FoxP3 Antibody (NB100-39002) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Anonymous IHC-P Mouse 10/06/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Spleen
• Species: Mouse
• Lot: D-4

FAQs for FoxP3 Antibody (NB100-39002). (Showing 1 - 5 of 5 FAQs).

1. Does FoxP3 antibodies comes in lyophilized form?
   • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
2. What the theoretical molecular weight for FoxP3 antibodies?
   • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
3. What is the dilution if it will be used in IHC? In other words, how many tests could obtained it used in paraffin based tissues?
   • The recommended dilution is 1:500 for IHC. It is difficult to predict how many tests can be made as the amount of antibody required will vary.
4. What can I use as positive controls for FOXP3 (NB100-39002) other than the mouse spleen tissue?
   • For FOXP3, the best tissues for detection of this protein would be appendix, lymph node, tonsil, spleen, or lung. You can see other tissue expression levels for this protein here: protein atlas site
5. What is the immunogen sequence of this FoxP3 antibody?
   • A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.

Bioinformatics

• Gene Symbol: FOXP3
• Entrez:
  • 20371(Mouse)
• Uniprot:
  • Q99JB6(Mouse)