FoxP3 Antibody


Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Number of Granzyme B+ and FoxP3+ cells infiltrating tumors. Representative images of IHC staining of FoxP3+ and Granzyme B+ cells in the peri-tumoral areas more
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - CCR10-expressing CD4+CD25+FOXP3+ Treg cells are enriched in the spinal cord after SCI. Double immunostaining of FOXP3 (red) and CCR10 (green) in the spinal cord more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on SK-MEL-28 cells with NB100-39002 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized more
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary more
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - FFPE bovine spleen tissue. Deparaffinization and antigen retrieval was carried out with PTlink from Dako, citrate buffer pH 6. Anti-Foxp3 antibody was used more
Western Blot: FoxP3 Antibody [NB100-39002] - Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and more
Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody [NB100-39002] - Transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. more
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - Transfectants were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then more
Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD8A mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection more
Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD4 mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection more

Product Details

Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-P, IP, Dual ISH-IHC, Single-Cell Western
1.0 mg/ml

FoxP3 Antibody Summary

A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Epitope affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Dual RNAscope ISH-IHC
  • Flow Cytometry 1:8000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation
  • Single Cell Western 100 ug/mL
  • Western Blot 1:2000
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in immunoblotting reported in scientific literature (PMID: 26485285). This FoxP3 antibody is validated for IHC-P from a verified customer review. SCW is validating using FoxP3-transfected HEK293T cells.
Reviewed Applications
Read 2 Reviews rated 4
NB100-39002 in the following applications:

Read Publications using
NB100-39002 in the following applications:

  • 3 publications
  • 3 publications
  • IHC
    7 publications
  • 11 publications
  • WB
    1 publication

Reactivity Notes

This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771). Bovine reativity reported from a verified customer review.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Epitope affinity purified

Alternate Names for FoxP3 Antibody

  • AIID
  • AIIDMGC141961
  • anti-human foxp3
  • anti-mouse foxp3
  • forkhead box P3
  • Forkhead Box Protein P3
  • foxp3 ihc
  • foxp3 paraffin
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked


FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: ELISA, ICC/IF, S-ELISA, WB
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Mu, Rt
Applications: Cell Depl, CyTOF-ready, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, InhibTFunc
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu, Po
Applications: Flow, ICC/IF, IF
Species: Mu
Applications: ELISA
Species: Hu
Applications: ELISA
Species: Hu
Applications: Block, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB
Species: Hu
Applications: AgAct, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, WB
Species: Ca, Hu
Applications: B/N, Flow, IP
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB

Publications for FoxP3 Antibody (NB100-39002)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow, ICC/IF, IHC, IHC-P, WB.

Filter By Application
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB100-39002 Applications Species
Garcia De Mattos Barbosa M, Lefferts Ar, Huynh D Et Al. TNFRSF13B genotypes control immune-mediated pathology by regulating the functions of innate B cells JCI insight Jul 20 2021 [PMID: 34283811] (IHC-P) IHC-P
Wang Z, Sun K, Xiao Y et al. Niraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models Sci Rep Feb 12 2019 [PMID: 30755715] (IHC-P, Human) IHC-P Human
Maisonneuve C, Tsang D, Foerster E Et Al. Nod1 Promotes Colorectal Carcinogenesis by Regulating the Immunosuppressive Functions of Tumor-Infiltrating Myeloid Cells SSRN Journal Jun 26 2020 (IHC, Mouse) IHC Mouse
Martin OCB, Bergonzini A, Lopez Chiloeches M et al. Influence of the microenvironment on modulation of the host response by typhoid toxin Cell reports Apr 6 2021 [PMID: 33826883] (IHC, Mouse) IHC Mouse
Matsumura-Kawashima M, Ogata K, Moriyama M et al. Secreted factors from dental pulp stem cells improve SjOgren’s syndrome via regulatory T cell-mediated immunosuppression Stem cell research & therapy Mar 16 2021 [PMID: 33726818] (IHC-P, Mouse) IHC-P Mouse
Gong Z, Jia H, Yu J et al. Nuclear FOXP3 inhibits tumor growth and induced apoptosis in hepatocellular carcinoma by targeting c-Myc Oncogenesis Oct 28 2020 [PMID: 33116119] (WB, IHC-P, Human) WB, IHC-P Human
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (Flow, Mouse)

Citation using the PE format of this antibody.
Flow Mouse
Radhakrishnan Rk, Thandi Rs, Tripathi D et Al. BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice JCI Insight Mar 12 2020 [PMID: 32161191] (Flow, Mouse) Flow Mouse
Steines L, Poth H, Schuster A et al. Anti-BAFF Treatment Interferes With Humoral Responses in a Model of Renal Transplantation in Rats Transplantation Jan 1 2020 [PMID: 31609901] (IHC-P) IHC-P
Wang P, Qi X, Xu G et al. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY) 2019 Sep 26 [PMID: 31557129] (ICC/IF, Mouse) ICC/IF Mouse
Show All 26 Publications.

Reviews for FoxP3 Antibody (NB100-39002) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Bovine, Goat, Sheep

Reviews using NB100-39002:
Filter by Applications
All Applications
Filter by Species
Bovine, Goat, Sheep
All Species
Images Ratings Applications Species Date Details
reviewed by:
Verified Customer
IHC-P Bovine, Goat, Sheep


Sample TestedFFPE spleen tissue
SpeciesBovine, Goat, Sheep


CommentsEpitope retrieval: PTlink from Dako with pH6 citrate buffer.
Primary antibody dilution: 1:100
DAB staining and hematoxylin contrast.
Immunohistochemistry-Paraffin FoxP3 NB100-39002
reviewed by:
Verified Customer
IHC-P Mouse 10/06/2015


Sample TestedMouse Spleen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxP3 Antibody (NB100-39002). (Showing 1 - 5 of 5 FAQs).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the dilution if it will be used in IHC? In other words, how many tests could obtained it used in paraffin based tissues?
    • The recommended dilution is 1:500 for IHC. It is difficult to predict how many tests can be made as the amount of antibody required will vary.
  4. What can I use as positive controls for FOXP3 (NB100-39002) other than the mouse spleen tissue?
    • For FOXP3, the best tissues for detection of this protein would be appendix, lymph node, tonsil, spleen, or lung. You can see other tissue expression levels for this protein here: protein atlas site
  5. What is the immunogen sequence of this FoxP3 antibody?
    • A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-39002AF350
Alexa Fluor 405 NB100-39002AF405
Alexa Fluor 488 NB100-39002AF488
Alexa Fluor 594 NB100-39002AF594
Alexa Fluor 647 NB100-39002AF647
Alexa Fluor 700 NB100-39002AF700
Alexa Fluor 750 NB100-39002AF750
Biotin NB100-39002B
DyLight 488 NB100-39002G
DyLight 550 NB100-39002R
DyLight 594 NB100-39002DL594
DyLight 650 NB100-39002C
DyLight 755 NB100-39002IR
HRP NB100-39002H
Janelia Fluor 549 NB100-39002JF549
Janelia Fluor 646 NB100-39002JF646
PE NB100-39002PE

Additional FoxP3 Products

Bioinformatics Tool for FoxP3 Antibody (NB100-39002)

Discover related pathways, diseases and genes to FoxP3 Antibody (NB100-39002). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for FoxP3 Antibody (NB100-39002)

Discover more about diseases related to FoxP3 Antibody (NB100-39002).

Pathways for FoxP3 Antibody (NB100-39002)

View related products by pathway.

PTMs for FoxP3 Antibody (NB100-39002)

Learn more about PTMs related to FoxP3 Antibody (NB100-39002).

Research Areas for FoxP3 Antibody (NB100-39002)

Find related products by research area.

Blogs on FoxP3.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.

FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Recent Reviews


Verified Customer
Application: IHC-P
Species: Bovine, Goat, Sheep

Verified Customer
Application: IHC-P
Species: Mouse


Gene Symbol FOXP3