FoxP3 Antibody - BSA Free

Images

 
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Number of Granzyme B+ and FoxP3+ cells infiltrating tumors. Representative images of IHC staining of FoxP3+ and Granzyme B+ cells in the peri-tumoral areas ...read more
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - CCR10-expressing CD4+CD25+FOXP3+ Treg cells are enriched in the spinal cord after SCI. Double immunostaining of FOXP3 (red) and CCR10 (green) in the spinal cord ...read more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on SK-MEL-28 cells with NB100-39002 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary ...read more
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - FFPE bovine spleen tissue. Deparaffinization and antigen retrieval was carried out with PTlink from Dako, citrate buffer pH 6. Anti-Foxp3 antibody was used ...read more
Western Blot: FoxP3 Antibody [NB100-39002] - Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and ...read more
Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody [NB100-39002] - Transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. ...read more
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary ...read more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - Transfectants were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of ...read more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD8A mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection ...read more
Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD4 mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection ...read more
Effects of anaphylatoxins on transcription factor phosphorylation in ARPE-19 cells.(D) Immunofluorescence staining of FoxP3. Upper panel: Lewis rat (albino) eye with experimental uveitis; lower panel: normal rat eye. ...read more
Number of Granzyme B+ and FoxP3+ cells infiltrating tumors. (B) Representative images of IHC staining of FoxP3+ and Granzyme B+ cells in the peri-tumoral areas in two mice characterized by low FoxP3+ and high Granzyme ...read more
CCR10-expressing CD4+CD25+FOXP3+ Treg cells are enriched in the spinal cord after SCI. (F) Double immunostaining of FOXP3 (red) and CCR10 (green) in the spinal cord sections after sham or SCI surgery. The merged images ...read more
FoxP3 was detected in immersion fixed MCF7 human breast cancer cell line using Rabbit anti-FoxP3 Affinity Purified Polyclonal Antibody conjugated to DyLight 488 (Catalog # NB100-39002G) (green) at 10 µg/mL overnight at ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP, WB, Dual ISH-IHC, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

FoxP3 Antibody - BSA Free Summary

Immunogen
A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXP3
Purity
Epitope affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Flow Cytometry 1:8000
  • Immunoblotting reported in scientific literature (PMID 26485285)
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400. Use reported by customer review
  • Immunoprecipitation
  • Single Cell Western 100 ug/mL
  • Western Blot 1:2000
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. SCW is validating using FoxP3-transfected HEK293T cells.
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-39002 in the following applications:

Publications
Read Publications using
NB100-39002 in the following applications:

Reactivity Notes

This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771). Bovine reativity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Epitope affinity purified

Alternate Names for FoxP3 Antibody - BSA Free

  • AIID
  • AIIDMGC141961
  • anti-human foxp3
  • anti-mouse foxp3
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • foxp3 ihc
  • foxp3 paraffin
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxP3 Antibody (NB100-39002)(53)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: FLOW, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, MI, WB.


Filter By Application
FLOW
(5)
ICC/IF
(5)
IF/IHC
(10)
IHC
(4)
IHC-Fr
(3)
IHC-P
(16)
MI
(1)
WB
(4)
All Applications
Filter By Species
Human
(12)
Mouse
(19)
Rat
(5)
All Species
Showing Publications 1 - 10 of 53. Show All 53 Publications.
Publications using NB100-39002 Applications Species
Zhou Y, Ju H, Hu Y et al. Tregs dysfunction aggravates postoperative cognitive impairment in aged mice Journal of neuroinflammation 2023-03-17 [PMID: 36932450] (IHC-Fr, Mouse) IHC-Fr Mouse
Yeong Song J, Guk Han M, Kim Y et al. Combination of local radiotherapy and anti-glucocorticoid-induced tumor necrosis factor receptor (GITR) therapy augments PD-L1 blockade-mediated anti-tumor effects in murine breast cancer model Radiotherapy and Oncology 2023-11-01 [PMID: 37925106] (IHC-P, Mouse) IHC-P Mouse
Pu S, Yang Z, Zhang X et al. Fermented cordyceps powder alleviates silica-induced pulmonary inflammation and fibrosis in rats by regulating the Th immune response Chinese medicine 2023-10-12 [PMID: 37828528] (IHC, Rat) IHC Rat
Doha ZO, Wang X, Calistri NL et al. MYC Deregulation and PTEN Loss Model Tumor and Stromal Heterogeneity of Aggressive Triple-Negative Breast Cancer Nat Commun 2023-09-13 [PMID: 37704631]
Shukla S, Saha T, Rama N et al. Ultra-high dose-rate proton FLASH improves tumor control Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology 2023-06-12 [PMID: 37315577]
Kagioka T, Itoh S, Hue M et al. Lithium carbonate accelerates the healing of apical periodontitis Research Square 2023-02-08 [PMID: 37193735] (IHC-Fr) IHC-Fr
Tsay TB, Chen PH, Li M et al. Monocyte Chemoattractant Protein-1-Supplemented Plasma Enhances Adiponectin and Adipogenesis-Related Gene Expression Stem cells and development 2023-01-02 [PMID: 36453206] (WB) WB
Sohn HS, Choi JW, Jhun J Et al. Tolerogenic nanoparticles induce type II collagen-specific regulatory T cells and ameliorate osteoarthritis Sci Adv 2022-11-25 [PMID: 36427299] (IF/IHC, Mouse)

Details:
Citation using the FITC version of this antibody.
IF/IHC Mouse
Itoh S, Kagioka T, Hue M et al. Lithium carbonate accelerates the healing of periapical periodontitis Research Square 2022-07-22 (IHC-Fr, Rat) IHC-Fr Rat
Show All 53 Publications.

Reviews for FoxP3 Antibody (NB100-39002) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Bovine, Goat, Sheep.

Reviews using NB100-39002:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Mouse
(1)
Bovine, Goat, Sheep
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin FoxP3 NB100-39002
Enlarge
4
reviewed by:
Verified Customer
IHC-P Bovine, Goat, Sheep 10/21/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFFPE spleen tissue
SpeciesBovine, Goat, Sheep
LotJ

Comments

CommentsEpitope retrieval: PTlink from Dako with pH6 citrate buffer.
Primary antibody dilution: 1:100
DAB staining and hematoxylin contrast.
Immunohistochemistry-Paraffin FoxP3 NB100-39002
Enlarge
4
reviewed by:
Verified Customer
IHC-P Mouse 10/06/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Spleen
SpeciesMouse
LotD-4

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxP3 Antibody (NB100-39002). (Showing 1 - 5 of 5 FAQs).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the dilution if it will be used in IHC? In other words, how many tests could obtained it used in paraffin based tissues?
    • The recommended dilution is 1:500 for IHC. It is difficult to predict how many tests can be made as the amount of antibody required will vary.
  4. What can I use as positive controls for FOXP3 (NB100-39002) other than the mouse spleen tissue?
    • For FOXP3, the best tissues for detection of this protein would be appendix, lymph node, tonsil, spleen, or lung. You can see other tissue expression levels for this protein here: protein atlas site
  5. What is the immunogen sequence of this FoxP3 antibody?
    • A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.

Secondary Antibodies

 

Isotype Controls

Additional FoxP3 Products

Research Areas for FoxP3 Antibody (NB100-39002)

Find related products by research area.

Blogs on FoxP3.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.

FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Verified Customer
10/21/2019
Application: IHC-P
Species: Bovine, Goat, Sheep

Verified Customer
10/06/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol FOXP3
Entrez
Uniprot