FoxP3 Antibody


Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - FFPE tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary more
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - FFPE tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - Transfectants were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of more
Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody [NB100-39002] - Transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. more
Western Blot: FoxP3 Antibody [NB100-39002] - Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and more
Western Blot: FoxP3 Antibody [NB100-39002] - Detection of immunoprecipitated FOXP3 from HA-tagged FOXP3 transfected Jurkat T antigen cells.
Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on SK-MEL-28 cells with FoxP3 Antibody NB100-39002 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then more

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
1.0 mg/ml

FoxP3 Antibody Summary

A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Epitope affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:2000
  • Flow Cytometry 1:8000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200 - 1:400
  • Immunohistochemistry-Paraffin 1:200 - 1:400
  • Immunoprecipitation
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 1 Review rated 4
NB100-39002 in the following applications:

Read Publications using
NB100-39002 in the following applications:

Reactivity Notes

This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771)

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.05% Sodium Azide
1.0 mg/ml
Epitope affinity purified

Alternate Names for FoxP3 Antibody

  • AIID
  • AIIDMGC141961
  • anti-human foxp3
  • anti-mouse foxp3
  • forkhead box P3
  • Forkhead Box Protein P3
  • foxp3 ihc
  • foxp3 paraffin
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked


FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
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Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu, Ca
Applications: Flow, IP, B/N
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Applications: WB, ICC/IF, IHC

Publications for FoxP3 Antibody (NB100-39002)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: Flow, ICC/IF, IHC, IHC-P.

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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB100-39002 Applications Species
Wang Z, Sun K, Xiao Y et al. Niraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models Sci Rep Feb 12 2019 [PMID: 30755715] (IHC-P, Human) IHC-P Human
Yu JW, Bhattacharya S, Yanamandra N et al. Tumor-immune profiling of murine syngeneic tumor models as a framework to guide mechanistic studies and predict therapy response in distinct tumor microenvironments. PLoS ONE. Nov 2 2018 [PMID: 30388137] (IHC-P, Mouse) IHC-P Mouse
Jiang Y, Cao Z, Ma H et al. Auricular Vagus Nerve Stimulation Exerts Antiinflammatory Effects and Immune Regulatory Function in a 6-OHDA Model of Parkinson's Disease. Neurochem. Res. Nov 1 2018 [PMID: 30311182] (ICC/IF, Rat) ICC/IF Rat
Schmidt T, Solimani F, Pollmann R et al. Th1/Th17 cell recognition of desmoglein 3 and bullous pemphigoid antigen 180 in lichen planus J. Allergy Clin. Immunol. 2018 Apr 04 [PMID: 29626572] (IHC-P, Human) IHC-P Human
Petrizzo A, Mauriello A, Luciano A, Rea D. Inhibition of tumor growth by cancer vaccine combined with metronomic chemotherapy and anti-PD-1 in a pre-clinical setting. Oncotarget. 2018 Jan 09 [PMID: 29423067] (IHC-P, Mouse) IHC-P Mouse
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (Flow, Mouse)

This citation used the PE form of this antbody
Flow Mouse
Sun J, Lian M, Ma H et al. Competing endogenous RNA network analysis of CD274, IL-10 and FOXP3 co-expression in laryngeal squamous cell carcinoma. Mol Med Rep 2017 Dec 19 [PMID: 29257349] (IHC) IHC
Busch C, Annamalai B, Abdusalamova K et al. Anaphylatoxins Activate Ca(2+), Akt/PI3-Kinase, and FOXO1/FoxP3 in the Retinal Pigment Epithelium. Front Immunol. 2017 Jun 30 [PMID: 28663750] (ICC/IF, Rat) ICC/IF Rat
Varga MG, Piazuelo MB, Romero-Gallo J et al. TLR9 activation suppresses inflammation in response to Helicobacter pylori infection. Am. J. Physiol. Gastrointest. Liver Physiol. Nov 1 2016 [PMID: 27758771] (IHC, Rat) IHC Rat
Zhang MZ, Yao B, Wang Y et al. Inhibition of cyclooxygenase-2 in hematopoietic cells results in salt-sensitive hypertension. J Clin Invest. [PMID: 26485285]
Show All 13 Publications.

Review for FoxP3 Antibody (NB100-39002) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-39002:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin FoxP3 NB100-39002
reviewed by:
IHC-P Mouse 10/06/2015


Sample TestedMouse Spleen

Product General Protocols

View specific protocols for FoxP3 Antibody (NB100-39002): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxP3 Antibody (NB100-39002). (Showing 1 - 5 of 5 FAQs).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the dilution if it will be used in IHC? In other words, how many tests could obtained it used in paraffin based tissues?
    • The recommended dilution is 1:500 for IHC. It is difficult to predict how many tests can be made as the amount of antibody required will vary.
  4. What can I use as positive controls for FOXP3 (NB100-39002) other than the mouse spleen tissue?
    • For FOXP3, the best tissues for detection of this protein would be appendix, lymph node, tonsil, spleen, or lung. You can see other tissue expression levels for this protein here: protein atlas site
  5. What is the immunogen sequence of this FoxP3 antibody?
    • A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-39002AF405
Alexa Fluor 488 Labeled NB100-39002AF488
Alexa Fluor 647 Labeled NB100-39002AF647
Alexa Fluor 700 Labeled NB100-39002AF700
Biotin Labeled NB100-39002B
DyLight 488 Labeled NB100-39002G
DyLight 550 Labeled NB100-39002R
DyLight 650 Labeled NB100-39002C
HRP Labeled NB100-39002H
PE Labeled NB100-39002PE

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Bioinformatics Tool for FoxP3 Antibody (NB100-39002)

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Research Areas for FoxP3 Antibody (NB100-39002)

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Blogs on FoxP3.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively,...  Read full blog post.

FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

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Recent Reviews


Application: IHC-P
Species: Mouse


Gene Symbol FOXP3