FoxP3 Antibody

Images

 
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was ...read more
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - FOXP3 antibody was tested in mouse spleen using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - IHC analysis of a formalin fixed paraffin embedded tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was ...read more
Western Blot: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - Detection of immunoprecipitated FOXP3 from HA-tagged FOXP3 transfected Jurkat T antigen cells.
Flow Cytometry: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - Transfectants from A were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by ...read more
Western Blot: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after ...read more
Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody [NB100-39002] - FOXP3 Antibody [NB100-39002] - The same transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
     

Genetic Strategies

   

Expression of Tagged Proteins

FoxP3 Antibody Summary

Immunogen
A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXP3
Purity
Epitope affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000
  • Flow Cytometry 1:8000
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:200 - 1:400
  • Immunohistochemistry-Paraffin 1:200 - 1:400
  • Immunoprecipitation
Application Notes
This FOXP3 antibody can be used for Immunoprecipitation, Immunohistochemistry paraffin embeded sections and Western blot analysis, where a band is seen ~48 kDa in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates. In IP transfected FOXP3-HA tagged proteins were used. In IHC-P, staining was observed in the nuclei of mouse spleen cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reviewed Applications
Read 1 Review rated 4
using
NB100-39002 in the following applications:

Publications
Read Publications using
NB100-39002 in the following applications:

Reactivity Notes

This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771)

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Epitope affinity purified

Alternate Names for FoxP3 Antibody

  • AIID
  • AIIDMGC141961
  • anti-human foxp3
  • anti-mouse foxp3
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • foxp3 ihc
  • foxp3 paraffin
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxP3 Antibody (NB100-39002)(6)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


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ICC/IF
(1)
IHC
(2)
IHC-P
(2)
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Human
(3)
Rat
(2)
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Review for FoxP3 Antibody (NB100-39002) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC-P.

Reviews using NB100-39002:
Filter by Applications
IHC-P
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin FoxP3 NB100-39002
Enlarge
reviewed by:
Anonymous
IHC-P Mouse 10/06/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Spleen
SpeciesMouse
LotD-4

Product General Protocols

View specific protocols for FoxP3 Antibody (NB100-39002): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxP3 Antibody (NB100-39002). (Showing 1 - 5 of 5 FAQs).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the dilution if it will be used in IHC? In other words, how many tests could obtained it used in paraffin based tissues?
    • The recommended dilution is 1:500 for IHC. It is difficult to predict how many tests can be made as the amount of antibody required will vary.
  4. What can I use as positive controls for FOXP3 (NB100-39002) other than the mouse spleen tissue?
    • For FOXP3, the best tissues for detection of this protein would be appendix, lymph node, tonsil, spleen, or lung. You can see other tissue expression levels for this protein here: protein atlas site
  5. What is the immunogen sequence of this FoxP3 antibody?
    • A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-39002AF405
Alexa Fluor 488 Labeled NB100-39002AF488
Alexa Fluor 647 Labeled NB100-39002AF647
Alexa Fluor 700 Labeled NB100-39002AF700
Biotin Labeled NB100-39002B
DyLight 488 Labeled NB100-39002G
DyLight 550 Labeled NB100-39002R
DyLight 650 Labeled NB100-39002C
HRP Labeled NB100-39002H
PE Labeled NB100-39002PE

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Bioinformatics Tool for FoxP3 Antibody (NB100-39002)

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Research Areas for FoxP3 Antibody (NB100-39002)

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Blogs on FoxP3.

FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively,...  Read full blog post.

FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

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Recent Reviews

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5
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4
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1
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Anonymous
10/06/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol FOXP3
Entrez
Uniprot