FoxP3 Antibody

Images

 
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Mouse spleen stained with FoxP3 antibody. IHC-P image submitted by a verified customer review.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Number of Granzyme B+ and FoxP3+ cells infiltrating tumors. Representative images of IHC staining of FoxP3+ and Granzyme B+ cells in the peri-tumoral areas ...read more
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - CCR10-expressing CD4+CD25+FOXP3+ Treg cells are enriched in the spinal cord after SCI. Double immunostaining of FOXP3 (red) and CCR10 (green) in the spinal cord ...read more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on SK-MEL-28 cells with NB100-39002 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized ...read more
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary ...read more
Immunohistochemistry: FoxP3 Antibody [NB100-39002] - Antibody was tested in mouse spleen using DAB with hematoxylin counterstain.
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - FFPE bovine spleen tissue. Deparaffinization and antigen retrieval was carried out with PTlink from Dako, citrate buffer pH 6. Anti-Foxp3 antibody was used ...read more
Western Blot: FoxP3 Antibody [NB100-39002] - Jurkat Tag cells were transfected with the expression vector (vector) or with the Foxp3 expression construct (Foxp3). 24 hours after transfection, cells were lysed and ...read more
Immunocytochemistry/ Immunofluorescence: FoxP3 Antibody [NB100-39002] - Transfectants were stained with anti-Foxp3 antibody and HRPO-conjugated (upper panel) or Cy3-conjugated (lower panels) secondary antibody. ...read more
Immunohistochemistry-Paraffin: FoxP3 Antibody [NB100-39002] - Tissue section of human tonsil using 1:200 dilution of rabbit anti-FoxP3 antibody. The staining was developed with HRP-labeled anti-rabbit IgG secondary ...read more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - Transfectants were permeabilized and stained with anti-Foxp3 antibody and FITC-conjugated secondary antibody. Cells were analyzed by flow cytometry. Percentage of ...read more
Flow Cytometry: FoxP3 Antibody [NB100-39002] - An intracellular stain was performed on MCF7 cells with FoxP3 Antibody NB100-39002PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD8A mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection ...read more
Dual RNAscope ISH-IHC: FoxP3 Antibody [NB100-39002] - CD4 mRNA (red) and FOXP3 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human breast cancer. ACD's Integrated Co-Detection ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, Flow, IB, ICC/IF, IHC, IHC-P, IP, Dual ISH-IHC, Single-Cell Western
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

FoxP3 Antibody Summary

Immunogen
A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FOXP3
Purity
Epitope affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Flow Cytometry 1:8000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
  • Immunoprecipitation
  • Single Cell Western 100 ug/mL
  • Western Blot 1:2000
Application Notes
Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Use in immunoblotting reported in scientific literature (PMID: 26485285). This FoxP3 antibody is validated for IHC-P from a verified customer review. SCW is validating using FoxP3-transfected HEK293T cells.
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-39002 in the following applications:

Publications
Read Publications using
NB100-39002 in the following applications:

  • 3 publications
  • 3 publications
  • IHC
    7 publications
  • 11 publications
  • WB
    1 publication

Reactivity Notes

This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771). Bovine reativity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Epitope affinity purified

Alternate Names for FoxP3 Antibody

  • AIID
  • AIIDMGC141961
  • anti-human foxp3
  • anti-mouse foxp3
  • DIETER
  • forkhead box P3
  • Forkhead Box Protein P3
  • foxp3 ihc
  • foxp3 paraffin
  • FoxP3
  • FOXP3delta7
  • immune dysregulation, polyendocrinopathy, enteropathy, X-linked
  • Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
  • IPEX
  • JM2
  • MGC141961
  • MGC141963
  • PIDX
  • PIDXMGC141963
  • SCURFIN
  • XPID
  • XPIDpolyendocrinopathy, enteropathy, X-linked

Background

FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FoxP3 Antibody (NB100-39002)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: Flow, ICC/IF, IHC, IHC-P, WB.


Filter By Application
Flow
(3)
ICC/IF
(3)
IHC
(7)
IHC-P
(11)
WB
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(12)
Rat
(3)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using NB100-39002 Applications Species
Garcia De Mattos Barbosa M, Lefferts Ar, Huynh D Et Al. TNFRSF13B genotypes control immune-mediated pathology by regulating the functions of innate B cells JCI insight Jul 20 2021 [PMID: 34283811] (IHC-P) IHC-P
Wang Z, Sun K, Xiao Y et al. Niraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models Sci Rep Feb 12 2019 [PMID: 30755715] (IHC-P, Human) IHC-P Human
Maisonneuve C, Tsang D, Foerster E Et Al. Nod1 Promotes Colorectal Carcinogenesis by Regulating the Immunosuppressive Functions of Tumor-Infiltrating Myeloid Cells SSRN Journal Jun 26 2020 (IHC, Mouse) IHC Mouse
Martin OCB, Bergonzini A, Lopez Chiloeches M et al. Influence of the microenvironment on modulation of the host response by typhoid toxin Cell reports Apr 6 2021 [PMID: 33826883] (IHC, Mouse) IHC Mouse
Matsumura-Kawashima M, Ogata K, Moriyama M et al. Secreted factors from dental pulp stem cells improve SjOgren’s syndrome via regulatory T cell-mediated immunosuppression Stem cell research & therapy Mar 16 2021 [PMID: 33726818] (IHC-P, Mouse) IHC-P Mouse
Gong Z, Jia H, Yu J et al. Nuclear FOXP3 inhibits tumor growth and induced apoptosis in hepatocellular carcinoma by targeting c-Myc Oncogenesis Oct 28 2020 [PMID: 33116119] (WB, IHC-P, Human) WB, IHC-P Human
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (Flow, Mouse)

Details:
Citation using the PE format of this antibody.
Flow Mouse
Radhakrishnan Rk, Thandi Rs, Tripathi D et Al. BCG vaccination reduces the mortality of Mycobacterium tuberculosis-infected type 2 diabetes mellitus mice JCI Insight Mar 12 2020 [PMID: 32161191] (Flow, Mouse) Flow Mouse
Steines L, Poth H, Schuster A et al. Anti-BAFF Treatment Interferes With Humoral Responses in a Model of Renal Transplantation in Rats Transplantation Jan 1 2020 [PMID: 31609901] (IHC-P) IHC-P
Wang P, Qi X, Xu G et al. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells Aging (Albany NY) 2019 Sep 26 [PMID: 31557129] (ICC/IF, Mouse) ICC/IF Mouse
Show All 26 Publications.

Reviews for FoxP3 Antibody (NB100-39002) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Bovine, Goat, Sheep
.

Reviews using NB100-39002:
Filter by Applications
IHC-P
(2)
All Applications
Filter by Species
Mouse
(1)
Bovine, Goat, Sheep
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-P Bovine, Goat, Sheep
 10/21/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedFFPE spleen tissue
SpeciesBovine, Goat, Sheep
LotJ

Comments

CommentsEpitope retrieval: PTlink from Dako with pH6 citrate buffer.
Primary antibody dilution: 1:100
DAB staining and hematoxylin contrast.
Immunohistochemistry-Paraffin FoxP3 NB100-39002
Enlarge
4
reviewed by:
Verified Customer
IHC-P Mouse 10/06/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Spleen
SpeciesMouse
LotD-4

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FoxP3 Antibody (NB100-39002). (Showing 1 - 5 of 5 FAQs).

  1. Does FoxP3 antibodies comes in lyophilized form?
    • FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
  2. What the theoretical molecular weight for FoxP3 antibodies?
    • The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
  3. What is the dilution if it will be used in IHC? In other words, how many tests could obtained it used in paraffin based tissues?
    • The recommended dilution is 1:500 for IHC. It is difficult to predict how many tests can be made as the amount of antibody required will vary.
  4. What can I use as positive controls for FOXP3 (NB100-39002) other than the mouse spleen tissue?
    • For FOXP3, the best tissues for detection of this protein would be appendix, lymph node, tonsil, spleen, or lung. You can see other tissue expression levels for this protein here: protein atlas site
  5. What is the immunogen sequence of this FoxP3 antibody?
    • A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-39002AF350
Alexa Fluor 405 NB100-39002AF405
Alexa Fluor 488 NB100-39002AF488
Alexa Fluor 594 NB100-39002AF594
Alexa Fluor 647 NB100-39002AF647
Alexa Fluor 700 NB100-39002AF700
Alexa Fluor 750 NB100-39002AF750
Biotin NB100-39002B
DyLight 488 NB100-39002G
DyLight 550 NB100-39002R
DyLight 594 NB100-39002DL594
DyLight 650 NB100-39002C
DyLight 755 NB100-39002IR
HRP NB100-39002H
Janelia Fluor 549 NB100-39002JF549
Janelia Fluor 646 NB100-39002JF646
PE NB100-39002PE

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Bioinformatics Tool for FoxP3 Antibody (NB100-39002)

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Blogs on FoxP3.

Meeting Report: 2nd International Antibody Validation Meeting
Bio-Techne brands Novus Biologicals® and R&D Systems® were proud to support the 2nd International Antibody Validation Meeting held at Bath University, on the 15-16 September, 2016. Almost 100 participants from around the world, including funde...  Read full blog post.

FOXP3
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function.  Distinctively, ...  Read full blog post.

FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of ...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Verified Customer
10/21/2019
Application: IHC-P
Species: Bovine, Goat, Sheep

Verified Customer
10/06/2015
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol FOXP3
Entrez
Uniprot