T-bet/TBX21 Antibody (39D)

Images

 
Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clone
39D
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

T-bet/TBX21 Antibody (39D) Summary

Immunogen
The immunogen for this antibody was raised against TBX21.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TBX21
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This 39D antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 ug/ml as a starting dilution for western blotting. Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 23691163).
Reviewed Applications
Read 1 Review rated 4
using
NBP1-43299 in the following applications:

Publications
Read Publication using
NBP1-43299 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS, pH 7.2
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for T-bet/TBX21 Antibody (39D)

  • Tbet
  • T-bet
  • TBETT-PET
  • TBLYM
  • TBLYMT-cell-specific T-box transcription factor T-bet
  • T-box 21
  • T-box expressed in T cells
  • T-box protein 21
  • T-box transcription factor TBX21
  • TBX21
  • T-PET
  • Transcription factor TBLYM

Background

The 39D monoclonal antibody reacts with mouse, rat and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for T-bet/TBX21 Antibody (NBP1-43299)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: ICC/IF, IHC.


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IHC
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Review for T-bet/TBX21 Antibody (NBP1-43299) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-43299:
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ICC
(1)
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Rat
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry T-bet/TBX21 NBP1-43299
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reviewed by:
Anonymous
ICC Rat 11/03/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedRat olfactory bulb, mouse olfactory bulb
SpeciesRat
Lot4340665

Comments

CommentsTissue has been fixed in formalin, blocked in Normal Goat Serum(NGS). Primary antibody was incubated for 48 hours at 4 degrees in 2.5% NGS + 0.1 % Triton X-100 in PBS (1:250 dilution for T-bet/Tbx21)

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for T-bet/TBX21 Antibody (NBP1-43299). (Showing 1 - 2 of 2 FAQ).

  1. Do you make a Tbx21 ab for FACS for porcine?
    • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.
  2. Can your TBX21 antibody (Catalog number NBP1-43299) be used for mouse frozen section by IF? I also wonder whether "ICC/IF"means it can only used for cell sample by IF.
    • Our T-bet/TBX21 antibody with catalogue number NBP1-43299 has been validated in-house for Western blotting and for immunoprecipitation, while IHC and ICC/IF were validated based on citation of the antibody in the publication detailed in the "reviews and publications" section on our product page. For future reference, ICC/IF does indeed refer to immunocytochemistry / immunofluorescence with cells. IHC refers to the use of tissue samples, with IHC-P indicating paraffin-embedded tissue, and IHC-Fr indicating frozen samples.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
11/03/2017
Application: ICC
Species: Rat

Bioinformatics

Gene Symbol TBX21
Entrez