Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and ...read more
Aqueous buffer, may contain carrier protein/stabilizer (proprietary).
0.09% Sodium Azide
Protein A or G purified
Western Blot 1:100-1:2000
This 39D antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 ug/ml as a starting dilution for western blotting. Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 23691163).
Read Publication using NBP1-43299 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
The 39D monoclonal antibody reacts with mouse, rat and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for T-bet/TBX21 Antibody (NBP1-43299). (Showing 1 - 2 of 2 FAQ).
Do you make a Tbx21 ab for FACS for porcine?
We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.
Can your TBX21 antibody (Catalog number NBP1-43299) be used for mouse frozen section by IF? I also wonder whether "ICC/IF"means it can only used for cell sample by IF.
Our T-bet/TBX21 antibody with catalogue number NBP1-43299 has been validated in-house for Western blotting and for immunoprecipitation, while IHC and ICC/IF were validated based on citation of the antibody in the publication detailed in the "reviews and publications" section on our product page. For future reference, ICC/IF does indeed refer to immunocytochemistry / immunofluorescence with cells. IHC refers to the use of tissue samples, with IHC-P indicating paraffin-embedded tissue, and IHC-Fr indicating frozen samples.