T-bet/TBX21 Antibody (39D) - BSA Free

Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IP
• Clone: 39D
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

T-bet/TBX21 Antibody (39D) - BSA Free Summary

• Immunogen: The immunogen for this antibody was raised against TBX21.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TBX21
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: This 39D antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 ug/ml as a starting dilution for western blotting. Use in Immunohistochemistry and Immunocytochemistry/Immunofluorescence was reported in a scientific publication (PMID: 23691163).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.2)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for T-bet/TBX21 Antibody (39D) - BSA Free

• Tbet
• T-bet
• TBETT-PET
• TBLYM
• TBLYMT-cell-specific T-box transcription factor T-bet
• T-box 21
• T-box expressed in T cells
• T-box protein 21
• T-box transcription factor TBX21
• TBX21
• T-PET
• Transcription factor TBLYM

Background

The 39D monoclonal antibody reacts with mouse, rat and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for T-bet/TBX21 Antibody (NBP1-43299)(3)

Publications using NBP1-43299

• Cho H, Park NJ, Ko J et al. Pellino-1 expression is associated with epidermal proliferation and enhanced Th17 cell infiltration in psoriatic lesions Experimental dermatology 2023-06-09 [PMID: 37291939]
• Bagnoli E, FitzGerald U. Mitral cells and the glucagon-like peptide 1 receptor: the sweet smell of success Eur. J. Neurosci. 2018-08-18 [PMID: 30120857] (IF/IHC, Mouse)
• Ma J, Dankulich-Nagrudny L, Lowe G. Cholecystokinin: An Excitatory Modulator of Mitral/Tufted Cells in the Mouse Olfactory Bulb. PLoS One 2013-05-15 [PMID: 23691163] (ICC/IF, IF/IHC, Mouse)

Review for T-bet/TBX21 Antibody (NBP1-43299) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer ICC Rat 11/03/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Rat olfactory bulb, mouse olfactory bulb
• Species: Rat
• Lot: 4340665

Comments

• Comments: Tissue has been fixed in formalin, blocked in Normal Goat Serum(NGS). Primary antibody was incubated for 48 hours at 4 degrees in 2.5% NGS + 0.1 % Triton X-100 in PBS (1:250 dilution for T-bet/Tbx21)

FAQs for T-bet/TBX21 Antibody (NBP1-43299). (Showing 1 - 2 of 2 FAQs).

1. Do you make a Tbx21 ab for FACS for porcine?
   • We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.
2. Can your TBX21 antibody (Catalog number NBP1-43299) be used for mouse frozen section by IF? I also wonder whether "ICC/IF"means it can only used for cell sample by IF.
   • Our T-bet/TBX21 antibody with catalogue number NBP1-43299 has been validated in-house for Western blotting and for immunoprecipitation, while IHC and ICC/IF were validated based on citation of the antibody in the publication detailed in the "reviews and publications" section on our product page. For future reference, ICC/IF does indeed refer to immunocytochemistry / immunofluorescence with cells. IHC refers to the use of tissue samples, with IHC-P indicating paraffin-embedded tissue, and IHC-Fr indicating frozen samples.

Bioinformatics

• Gene Symbol: TBX21