GITR/TNFRSF18 Antibody (110416) [Unconjugated]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, B/N, CyTOF-ready, ELISA(Cap)
Clone
110416
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GITR/TNFRSF18 Antibody (110416) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human GITR/TNFRSF18
Gln26-Glu161 (Thr43Ala)
Accession # Q9Y5U5
Specificity
Detects human GITR in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 4‑1BB, recombinant mouse
(rm) 4‑1BB, rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rhDR6, rhEDAR, rmEDAR, rhFas, rmFAS, rmGITR, rhHVEM, rhLymphotoxin R beta, rmLymphotoxin R beta, rhNGF R, rhOPG, rmOPG, rhRANK, rmRANK, rhTAJ, rhTNF RI, rmTNF RI, rhTNF RII, or rmTNF RII.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF18
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Blockade of Receptor-ligand Interaction 1-2 ug/mL
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Detection: Human GITR/TNFRSF18 Biotinylated Antibody (Catalog number BAF689)
Standard: Recombinant Human GITR/TNFRSF18 Fc Chimera (Catalog number 689-GR)
Publications
Read Publications using
MAB689 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GITR/TNFRSF18 Antibody (110416) [Unconjugated]

  • Activation-inducible TNFR family receptor
  • AITR
  • AITRTNF receptor superfamily activation-inducible protein
  • CD357 antigen
  • CD357
  • GITR
  • GITR-D
  • GITRtumor necrosis factor receptor superfamily member 18
  • Glucocorticoid-induced TNFR-related protein
  • TNFRSF18
  • tumor necrosis factor receptor superfamily, member 18

Background

GITR (glucocorticoid-induced tumor necrosis factor receptor, also named AITR, activation-inducible TNF R family member), is a 228 amino acid (aa) type I transmembrane protein belonging to the TNF R family and has been designated TNFRSF18. The GITR cytoplasmic domain has striking homology with the cytoplasmic domain of 4-1BB and CD27. Human GITR shares 55% homology with murine GITR. GITR is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen, and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is
up-regulated by antigen stimulation or by treatment with anti-CD3 plus anti-CD28, or PMA plus ionomycin. Human GITR ligand was identified from human umbilical vein endothelial cells and is a 177 aa polypeptide belonging to the TNF superfamily (TNFSF18). Ligation of GITR can activate NF-kappa B through TRAF2, and protect T cells from TCR activation-induced cell death. It has been proposed that GITR ligand and GITR may modulate T lymphocyte functions.

  1. Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
  2. Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
  3. Gurney, A.L. et al. (1999) Current Biology 9:215.
  4. Kwon, B. et al. (1999) Current Opinion in Immunology 11:340.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GITR/TNFRSF18 Antibody (MAB689)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, ICC, Neutralization.


Filter By Application
Flow Cytometry
(6)
ICC
(1)
Neutralization
(2)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB689 Applications Species
A Heine, SAE Held, J Schulte-Sc, JFA Wolff, K Klee, T Ulas, NA Schmacke, SN Daecke, K Riethausen, JL Schultze, P Brossart Generation and functional characterization of MDSC-like cells Oncoimmunology, 2017;6(4):e1295203. 2017 [PMID: 28507805] (ICC, Human) ICC Human
Lee F, Luevano M, Veys P, Yong K, Madrigal A, Shaw B, Saudemont A The effects of CAMPATH-1H on cell viability do not correlate to the CD52 density on the cell surface. PLoS ONE, 2014;9(7):e103254. 2014 [PMID: 25050704] (Flow Cytometry, Human) Flow Cytometry Human
Schmiedel, Benjamin, Werner, Antje, Steinbacher, Julia, Nuebling, Tina, Buechele, Corina, Grosse-Hovest, Ludger, Salih, Helmut R Generation and preclinical characterization of a Fc-optimized GITR-Ig fusion protein for induction of NK cell reactivity against leukemia. Mol Ther, 2013;21(4):877-86. 2013 [PMID: 23380816] (Neutralization, Human) Neutralization Human
Mandapathil M, Szczepanski M, Harasymczuk M, Ren J, Cheng D, Jackson E, Gorelik E, Johnson J, Lang S, Whiteside T CD26 expression and adenosine deaminase activity in regulatory T cells (Treg) and CD4(+) T effector cells in patients with head and neck squamous cell carcinoma. Oncoimmunology, 2012;1(5):659-669. 2012 [PMID: 22934258] (Flow Cytometry, Human) Flow Cytometry Human
Savage ND, De Boer T, Walburg KV, Joosten SA, van Meijgaarden K, Geluk A, Ottenhoff TH Human anti-inflammatory macrophages induce Foxp3+ GITR+ CD25+ regulatory T cells, which suppress via membrane-bound TGFbeta-1. J. Immunol., 2008;181(3):2220-6. 2008 [PMID: 18641362] (Flow Cytometry, Human) Flow Cytometry Human
Baltz KM, Krusch M, Bringmann A, Brossart P, Mayer F, Kloss M, Baessler T, Kumbier I, Peterfi A, Kupka S, Kroeber S, Menzel D, Radsak MP, Rammensee HG, Salih HR Cancer immunoediting by GITR (glucocorticoid-induced TNF-related protein) ligand in humans: NK cell/tumor cell interactions. FASEB J., 2007;21(10):2442-54. 2007 [PMID: 17360848] (Flow Cytometry, Human) Flow Cytometry Human
Birebent B, Lorho R, Lechartier H, de Guibert S, Alizadeh M, Vu N, Beauplet A, Robillard N, Semana G Suppressive properties of human CD4+CD25+ regulatory T cells are dependent on CTLA-4 expression. Eur. J. Immunol., 2004;34(12):3485-96. 2004 [PMID: 15484187] (Flow Cytometry, Human) Flow Cytometry Human
Godfrey WR, Spoden DJ, Ge YG, Baker SR, Liu B, Levine BL, June CH, Blazar BR, Porter SB Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood, 2005;105(2):750-8. 2005 [PMID: 15374887] (Neutralization, Human) Neutralization Human
Zheng SG, Wang JH, Gray JD, Soucier H, Horwitz DA Natural and induced CD4+CD25+ cells educate CD4+CD25- cells to develop suppressive activity: the role of IL-2, TGF-beta, and IL-10. J. Immunol., 2004;172(9):5213-21. 2004 [PMID: 15100259] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol TNFRSF18
Uniprot