Mouse IL-17 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Mouse IL-17 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il17a

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DY421 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-17 DuoSet ELISA, 15 Plate

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Mouse Interleukin 17 (IL-17; also known as IL-17A and CTLA-8) is a 21 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines containing a cysteine-knot fold (1-3). Its sequence was originally isolated from an activated hybridoma created from the fusion of a mouse cytotoxic and rat T cell lymphoma cell line (2-5). It is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 15 kDa, 133 aa mature segment (5). In both mouse and human, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 35-38 kDa homodimer, and a 45-48 kDa heterodimer with IL-17F (6, 7). Mature mouse IL-17A is 61% and 89% aa identical to human and rat IL-17A, respectively (4, 5, 8). While rodent and human mature sequences show modest aa sequence identity, human IL-17 is active on both mouse and rat cells (5, 9). Cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27-gamma δ T cells, CD1+NK1.1- iNKT cells and CD3- CD4+ LTi-like cells (3, 5, 6, 10-12). 
A high affinity receptor for mouse IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 13). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA (14, 15). Both receptors are needed for IL-17A and IL-17A/F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 16). 
IL-17 is best known for its participation in the recruitment and survival of neutrophils (3, 10, 17, 18). Its induction was initially described to be the result of antigen stimulation of dentritic cells, resulting in IL-23 secretion. In a TCR-independent event, IL-23 induces T cell production of IL-17 (3). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and SCF (membrane form), an effect that increases neutrophil differentiation and activation. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating neutrophil numbers (17). In the tissues, IL-17 seems to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-alpha, IL-1 beta and IL-6 production (19). TNF-alpha and IL-1 beta then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (20). IL-17 further contributes to neutrophil influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (3, 9). IL-17 effects are not limited to neutrophils. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (18).

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Publications for IL-17/IL-17A (DY421)(147)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: ELISA Standard.


Filter By Application
ELISA Standard
(1)
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Filter By Species
Mouse
(143)
Rat
(4)
All Species
Showing Publications 1 - 10 of 147. Show All 147 Publications.
Publications using DY421 Applications Species
KI Lee, JS Bae, EH Kim, JH Kim, L Lyu, YJ Chung, JH Mo Strain-Specific Differences in House Dust Mite (Dermatophagoides farinae)-Induced Mouse Models of Allergic Rhinitis Clin Exp Otorhinolaryngol, 2020;0(0):. 2020 [PMID: 32407614] (Mouse) Mouse
L Li, Z Fang, X Liu, W Hu, W Lu, YK Lee, J Zhao, H Zhang, W Chen Lactobacillus reuteri attenuated allergic inflammation induced by HDM in the mouse and modulated gut microbes PLoS ONE, 2020;15(4):e0231865. 2020 [PMID: 32315360] (Mouse) Mouse
SH Kim, JH Hong, WK Yang, JH Geum, HR Kim, SY Choi, YM Kang, HJ An, YC Lee Herbal Combinational Medication of Glycyrrhiza glabra, Agastache rugosa Containing Glycyrrhizic Acid, Tilianin Inhibits Neutrophilic Lung Inflammation by Affecting CXCL2, Interleukin-17/STAT3 Signal Pathways in a Murine Model of COPD Nutrients, 2020;12(4):. 2020 [PMID: 32230838] (Mouse) Mouse
LRC de Oliveir, LAN Mimura, TFC Fraga-Silv, LLW Ishikawa, AAH Fernandes, SFG Zorzella-P, A Sartori Calcitriol Prevents Neuroinflammation and Reduces Blood-Brain Barrier Disruption and Local Macrophage/Microglia Activation Front Pharmacol, 2020;11(0):161. 2020 [PMID: 32226379] (Mouse) Mouse
J Hrdý, J Alard, A Couturier-, O Boulard, D Boutillier, M Delacre, C Lapadatesc, A Cesaro, P Blanc, B Pot, B Ryffel, M Chamaillar, C Grangette Lactobacillus reuteri 5454 and Bifidobacterium animalis ssp. lactis 5764 improve colitis while differentially impacting dendritic cells maturation and antimicrobial responses Sci Rep, 2020;10(1):5345. 2020 [PMID: 32210304] (Mouse) Mouse
N Taghipour, N Mosaffa, HA Aghdaei, M Rostami-Ne, JV Weinstock, S Shahnavaz, MR Zali Immunomodulatory effect of Syphacia obvelata in treatment of experimental DSS-induced colitis in mouse model Sci Rep, 2019;9(1):19127. 2019 [PMID: 31836772] (Mouse) Mouse
A Suhail, ZA Rizvi, P Mujagond, SA Ali, P Gaur, M Singh, V Ahuja, A Awasthi, CV Srikanth DeSUMOylase SENP7-Mediated Epithelial Signaling Triggers Intestinal Inflammation via Expansion of Gamma-Delta T Cells Cell Rep, 2019;29(11):3522-3538.e7. 2019 [PMID: 31825833] (Mouse) Mouse
MC de Rezende, JMP Moreira, LLM Fernandes, VF Rodrigues, D Negrão-Cor Strongyloides venezuelensis-infection alters the profile of cytokines and liver inflammation in mice co-infected with Schistosoma mansoni Cytokine, 2019;127(0):154931. 2019 [PMID: 31783260] (Mouse) Mouse
JM O'Hara, NS Redhu, E Cheung, NG Robertson, I Patik, SE Sayed, CM Thompson, M Herd, KB Lucas, E Conaway, CC Morton, DL Farber, R Malley, BH Horwitz Generation of protective pneumococcal-specific nasal resident memory CD4+ T cells via parenteral immunization Mucosal Immunol, 2019;0(0):. 2019 [PMID: 31659300] (Mouse) Mouse
W Zhou, Y Yang, C Mei, P Dong, S Mu, H Wu, Y Zhou, Y Zheng, F Guo, JQ Yang Inhibition of Rho-Kinase Downregulates Th17 Cells and Ameliorates Hepatic Fibrosis by Schistosoma japonicum Infection Cells, 2019;8(10):. 2019 [PMID: 31623153] (Mouse) Mouse
Show All 147 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for IL-17/IL-17A (DY421). (Showing 1 - 3 of 3 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the following link to see the available fluors: http://www.novusbio.com/support/custom-antibody-labeling.html The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).
  3. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

Biotin DY421
Biotin DY421

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Bioinformatics

Gene Symbol Il17a