Mouse IL-17 DuoSet ELISA, 15 Plate

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications ELISA
Conjugate
Biotin

Order Details

Mouse IL-17 DuoSet ELISA, 15 Plate Summary

Source
N/A
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Il17a

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using
DY421 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-17 DuoSet ELISA, 15 Plate

  • CTLA8
  • CTLA-8
  • CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
  • Cytotoxic T-lymphocyte-associated antigen 8
  • IL17
  • IL-17
  • IL17A
  • IL-17A
  • IL-17Acytotoxic T-lymphocyte-associated protein 8
  • IL-17CTLA-8
  • IL17interleukin-17A
  • interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
  • interleukin 17A

Background

Mouse Interleukin 17 (IL-17; also known as IL-17A and CTLA-8) is a 21 kDa, variably glycosylated polypeptide that belongs to the IL-17 family of cytokines containing a cysteine-knot fold (1-3). Its sequence was originally isolated from an activated hybridoma created from the fusion of a mouse cytotoxic and rat T cell lymphoma cell line (2-5). It is synthesized as a 158 amino acid (aa) precursor that contains a 25 aa signal sequence and a 15 kDa, 133 aa mature segment (5). In both mouse and human, there is one conserved N-linked glycosylation site that likely contributes 5 kDa to its native molecular weight. IL-17A forms both a 35-38 kDa homodimer, and a 45-48 kDa heterodimer with IL-17F (6, 7). Mature mouse IL-17A is 61% and 89% aa identical to human and rat IL-17A, respectively (4, 5, 8). While rodent and human mature sequences show modest aa sequence identity, human IL-17 is active on both mouse and rat cells (5, 9). Cells known to produce IL-17 are the CD4+ Th17 T cells, Paneth cells, GR1+CD11b+ myeloid suppressor cells, CD27-gamma δ T cells, CD1+NK1.1- iNKT cells and CD3- CD4+ LTi-like cells (3, 5, 6, 10-12). 
A high affinity receptor for mouse IL-17 has been reported, and appears to be a heteromultimer of IL-17RA and IL-17RC, likely in a 2:1 ratio (1). IL-17RA is a 130 kDa, type I transmembrane glycoprotein that bears no resemblance to members of the cytokine, TNF or immunoglobulin receptor superfamily (2, 10, 13). IL-17RC is also a type I transmembrane protein, approximately 90-95 kDa in size, that shares less than 30% aa identity with IL-17RA (14, 15). Both receptors are needed for IL-17A and IL-17A/F activity. The two receptors appear to form a functional association following ligand binding to IL-17RA (1, 16). 
IL-17 is best known for its participation in the recruitment and survival of neutrophils (3, 10, 17, 18). Its induction was initially described to be the result of antigen stimulation of dentritic cells, resulting in IL-23 secretion. In a TCR-independent event, IL-23 induces T cell production of IL-17 (3). Once secreted, IL-17 in the bone marrow would seem to induce stromal/fibroblast expression of both G-CSF and SCF (membrane form), an effect that increases neutrophil differentiation and activation. IL-17 may complement this by directly blocking neutrophil apoptosis, promoting greater circulating neutrophil numbers (17). In the tissues, IL-17 seems to promote neutrophil extravasation, principally through its effects on macrophages and endothelial cells (EC). On macrophages, IL-17 induces TNF-alpha , IL-1 beta and IL-6 production (19). TNF-alpha and IL-1 beta then act on local ECs to induce G-CSF secretion, an effect that is potentiated by IL-17 (20). IL-17 further contributes to neutrophil influx by inducing EC CXC chemokine release and NO production, which may increase vascular permeability (3, 9). IL-17 effects are not limited to neutrophils. In synovial joints, IL-17 upregulates RANKL expression on osteoblasts. This provides a stimulus for osteoclast formation and subsequent bone resorption (18).

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Publications for IL-17/IL-17A (DY421)(166)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 1 application: ELISA Standard.


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ELISA Standard
(1)
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Mouse
(162)
Rat
(4)
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Showing Publications 1 - 10 of 166. Show All 166 Publications.
Publications using DY421 Applications Species
M Di Filippo, A Mancini, L Bellingacc, L Gaetani, P Mazzocchet, T Zelante, L La Barbera, A De Luca, M Tantucci, A Tozzi, V Durante, M Sciaccalug, A Megaro, D Chiasserin, N Salvadori, V Lisetti, E Portaccio, C Costa, P Sarchielli, MP Amato, L Parnetti, MT Viscomi, L Romani, P Calabresi Interleukin-17 affects synaptic plasticity and cognition in an experimental model of multiple sclerosis Cell Reports, 2021;37(10):110094. 2021 [PMID: 34879272] (Mouse) Mouse
M Kokoszynsk, ND Ubags, JJ Bivona, S Ventrone, LF Reed, AE Dixon, MJ Wargo, ME Poynter, BT Suratt Storage conditions of high-fat diets affect pulmonary inflammation Physiological Reports, 2021;9(22):e15116. 2021 [PMID: 34822216] (Mouse) Mouse
J Hu, C Wang, X Huang, S Yi, S Pan, Y Zhang, G Yuan, Q Cao, X Ye, H Li Gut microbiota-mediated secondary bile acids regulate dendritic cells to attenuate autoimmune uveitis through TGR5 signaling Cell Reports, 2021;36(12):109726. 2021 [PMID: 34551302] (Mouse) Mouse
HJ Jung, YK Ko, WS Shim, HJ Kim, DY Kim, CS Rhee, MK Park, DH Han Diesel exhaust particles increase nasal symptoms and IL-17A in house dust mite-induced allergic mice Scientific Reports, 2021;11(1):16300. 2021 [PMID: 34381060] (Mouse) Mouse
A Chaffey, G Hamonic, D Chand, GK Mutwiri, HL Wilson The Adjuvants Polyphosphazene (PCEP) and a Combination of Curdlan Plus Leptin Promote a Th17-Type Immune Response to an Intramuscular Vaccine in Mice Vaccines, 2021;9(5):. 2021 [PMID: 34069081] (Mouse) Mouse
AM Antonson, AD Kenney, HJ Chen, KN Corps, JS Yount, TL Gur Moderately pathogenic maternal influenza A virus infection disrupts placental integrity but spares the fetal brain Brain, Behavior, and Immunity, 2021;0(0):. 2021 [PMID: 33989741] (Mouse) Mouse
D Arsenijevi, B Stojanovic, J Milovanovi, A Arsenijevi, M Simic, M Pergal, I Kodranov, O Cvetkovic, D Vojvodic, E Ristanovic, D Manojlovic, M Milovanovi, N Arsenijevi Hepatoprotective Effect of Mixture of Dipropyl Polysulfides in Concanavalin A-Induced Hepatitis Nutrients, 2021;13(3):. 2021 [PMID: 33809904] (Mouse) Mouse
B Wang, Q Kong, S Cui, X Li, Z Gu, J Zhao, H Zhang, W Chen, G Wang Bifidobacterium adolescentis Isolated from Different Hosts Modifies the Intestinal Microbiota and Displays Differential Metabolic and Immunomodulatory Properties in Mice Fed a High-Fat Diet Nutrients, 2021;13(3):. 2021 [PMID: 33801119] (Mouse) Mouse
SJ Gurczynski, NL Pereira, SM Hrycaj, C Wilke, RL Zemans, BB Moore Stem cell transplantation uncovers TDO-AHR regulation of lung dendritic cells in herpesvirus-induced pathology JCI Insight, 2021;6(2):. 2021 [PMID: 33491663] (Mouse) Mouse
A Fajstova, N Galanova, S Coufal, J Malkova, M Kostovcik, M Cermakova, H Pelantova, M Kuzma, B Sediva, T Hudcovic, T Hrncir, H Tlaskalova, M Kverka, K Kostovciko Diet Rich in Simple Sugars Promotes Pro-Inflammatory Response via Gut Microbiota Alteration and TLR4 Signaling Cells, 2020;9(12):. 2020 [PMID: 33339337] (Mouse) Mouse
Show All 166 Publications.

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FAQs for IL-17/IL-17A (DY421). (Showing 1 - 2 of 2 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
    • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the following link to see the available fluors: http://www.novusbio.com/support/custom-antibody-labeling.html The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

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Bioinformatics

Gene Symbol Il17a