IL-7R alpha/CD127 Antibody (R34-34)

Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34

Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of R34-34-transfected Cos7 cells

Immunoprecipitation: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - R34-34 antigen from 125I-surface labelled Pre-Alp cells

• Reactivity: Hu, Ca
• Applications: Flow, IP, B/N
• Clone: R34-34
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IL-7R alpha/CD127 Antibody (R34-34) Summary

• Immunogen: IL7R Alpha/CD127 was used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL7R
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500
  • Block/Neutralize

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Dendritics and distributed by Novus Biologicals in North America and other select countries. To order in the following countries: Germany, Finland, United Kingdom, Norway, Denmark, Switzerland, Austria, Japan, and South Korea, please contact Dendritics for additional ordering information.

Alternate Names for IL-7R alpha/CD127 Antibody (R34-34)

• CD127 antigen
• CD127
• CD127ILRA
• CDW127
• IL-7 R alpha
• IL-7 receptor subunit alpha
• IL7R alpha
• IL-7R alpha
• IL-7R subunit alpha
• IL7R
• IL7RA
• IL-7Ra
• IL-7R-alpha
• interleukin 7 receptor alpha chain
• interleukin 7 receptor isoform H5-6
• interleukin 7 receptor
• interleukin-7 receptor subunit alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-7R alpha/CD127 Antibody (DDX0700P-100)(40)

Publications using DDX0700P-100

• Faller EM et al. IL-7 receptor recovery on CD8 T-cells isolated from HIV+ patients is inhibited by the HIV Tat protein. PLoS One 2014 Jul 17 [PMID: 25033393]
• Larbi et al. Generation of multipotent early lymphoid progenitors from human embryonic stem cells. Stem Cells Dev 2014 Dec 15 [PMID: 24955741]
• Munneke JM et al. Activated innate lymphoid cells are associated with a reduced susceptibility to graft-versus-host disease. Blood 2014 Jul 31 [PMID: 24855210]
• Forsberg A et al. GATA binding protein 3+ group 2 innate lymphoid cells are present in cord blood and in higher proportions in male than in female neonates. J Allergy Clin Immunol 2014 Jul [PMID: 24636083]
• Peguillet I et al. High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer. Cancer Res 2014 Apr 15 [PMID: 24535711]
• Sereti I et al. Decreases in Colonic and Systemic Inflammation inChronic HIV Infection after IL-7 Administration. PLoS Pathog 2014 Jan 30 [PMID: 24497828]
• Compeer EB et al. Tubulation of endosomal structures in human dendritic cells by Toll-like receptor ligation and lymphocyte contact accompanies antigen cross-presentation. J Biol Chem 2014 Jan 3 [PMID: 24235148]
• Yu X et al. TNF superfamily member TL1A elicits type 2 innate lymphoid cells at mucosal barriers. Mucosal Immunol 2014 May [PMID: 24220298]
• Lozza L et al. Crosstalk between human DC subsets promotes antibacterial activity and CD8+ T-cell stimulation in response to bacille Calmette-Guerin. Eur J Immunol 2014 Jan [PMID: 24114554]
• Ponchel F et al. An immunological biomarker to predict MTX response in early RA. Ann Rheum Dis 2014 Nov [PMID: 23989988]
• Bernink JH et al. Human type 1 innate lymphoid cells accumulate in inflamed mucosal tissues. Nat Immunol 2013 Mar [PMID: 23334791]
• Luckashenak N et al. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol 2013 Jan 1 [PMID: 23209325]
• Ghazawi FM et al. IL-7 downregulates IL-7Ralpha expression in human CD8 T cells by two independent mechanisms. Immunol Cell Biol 2013 Feb [PMID: 23207282]
• Yatim A et al. NOTCH1 nuclear interactome reveals key regulators of its transcriptional activity and oncogenic function. Mol Cell 2012 Nov 9 [PMID: 23022380]
• Petrovas C et al. CD4 T follicular helper cell dynamics during SIV infection. J Clin Invest 2012 Sep [PMID: 22922258]
• Noakes PS et al. Increased intake of oily fish in pregnancy: effects on neonatal immune responses and on clinical outcomes in infants at 6 mo. Am J Clin Nutr 2012 Feb [PMID: 22218160]
• Young CD et al. HIV infection of thymocytes inhibits IL-7 activity without altering CD127 expression. Retrovirology 2011 Sep 16 [PMID: 21920046]
• Mjosberg JM et al. Human IL-25- and IL-33-responsive type 2 innate lymphoid cells are defined by expression of CRTH2 and CD161. Nat Immunol 2011 Sep 11 [PMID: 21909091]
• Pankewycz O et al. Low-dose rabbit antithymocyte globulin induction therapy results in prolonged selective lymphocyte depletion irrespective of maintenance immunosuppression. Transplant Proc 2011 Mar [PMID: 21440734]
• van Roon J et al. Numbers of CD25+Foxp3+ T cells that lack the IL-7 receptor are increased intra-articularly and have impaired suppressive function in RA patients. Rheumatology (Oxford) 2010 Nov [PMID: 20693259]
• Engram J et al. Lineage-specific T-cell reconstitution following in vivo CD4+ and CD8+ lymphocyte depletion in nonhuman primates. Blood 2010 Aug 5 [PMID: 20484087]
• Crawley AM et al. Soluble IL-7R alpha (sCD127) inhibits IL-7 activity and is increased in HIV infection. J Immunol 2010 May 1 [PMID: 20304824]
• Presicce P et al. Association of two clones allows for optimal detection of human FOXP3. Cytometry A 2010 Jun [PMID: 20162533]
• Valencia J et al. Transient beta-catenin stabilization modifies lineage output from human thymic CD34+CD1a- progenitors. J Leukoc Biol 2010 Mar [PMID: 19952356]
• Portevin D et al. Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy. J Transl Med 2009 Sep 24 [PMID: 19778420]
• Vudattu NK et al. Increased numbers of IL-7 receptor molecules on CD4+CD25-CD107a+ T-cells in patients with autoimmune diseases affecting the central nervous system. PLoS One 2009 Aug 6 [PMID: 19657390]
• Sauce D et al. Upregulation of interleukin 7 receptor alpha and programmed death 1 marks an epitope-specific CD8+ T-cell response that disappears following primary Epstein-Barr virus infection. J Virol 2009 Sep [PMID: 19605492]
• Luczynski W et al. Diminished expression of ICOS, GITR and CTLA-4 at the mRNA level in T regulatory cells of children with newly diagnosed type 1 diabetes. Acta Biochim Pol 2009 [PMID: 19547759]
• Faller EM et al. IL-7 and the HIV Tat protein act synergistically to down-regulate CD127 expression on CD8 T cells. Int Immunol 2009 Mar [PMID: 19147839]
• Sharma TS et al. CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children. PLoS One 2008 [PMID: 19096522]
• Mason RD et al. Low pre-infection levels and loss of central memory CD4+ T cells may predict rapid progression in SIV-infected pigtail macaques. Virology 2008 Nov 10 [PMID: 18835006]
• Johnson SE et al. IL-7 activates the phosphatidylinositol 3-kinase/AKT pathway in normal human thymocytes but not normal human B cell precursors. J Immunol 2008 Jun 15 [PMID: 18523275]
• Zonios DI et al. Idiopathic CD4+ lymphocytopenia: natural history and prognostic factors. Blood 2008 Jul 15 [PMID: 18456875]
• Esquerre M et al. Human regulatory T cells inhibit polarization of T helper cells toward antigen-presenting cells via a TGF-beta-dependent mechanism. Proc Natl Acad Sci U S A 2008 Feb 19 [PMID: 18268354]
• Esendagli G et al. Malignant and non-malignant lung tissue areas are differentially populated by natural killer cells and regulatory T cells in non-small cell lung cancer. Lung Cancer 2008 Jan [PMID: 17825949]
• Scupoli MT et al. Interleukin 7 requirement for survival of T-cell acute lymphoblastic leukemia and human thymocytes on bone marrow stroma. Haematologica 2007 Feb [PMID: 17296584]
• Hartigan-O'Connor DJ et al. Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells. J Immunol Methods 2007 Jan 30 [PMID: 17173927]
• Higgins J et al. Effects of lymphocyte isolation and timing of processing on detection of CD127 expression on T cells in human immunodeficiency virus-infected patients. Clin Diagn Lab Immunol 2005 Jan [PMID: 15643013]
• Ito T et al. Differential regulation of human blood dendritic cell subsets by IFNs. J Immunol 2001 Mar 1 [PMID: 11207245]
• Pandrau-Garcia D et al. Growth inhibitory and agonistic signals of interleukin-7 (IL-7) can be mediated through the CDw127 IL-7 receptor Blood 1994 Jun 15 [PMID: 8204885]

Bioinformatics

• Gene Symbol: IL7R