IL-7R alpha/CD127 Antibody (R34-34)

Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of PBL with R34-34

Flow Cytometry: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - Facs staining of R34-34-transfected Cos7 cells

Immunoprecipitation: IL-7 R alpha/CD127 Antibody (R34-34) [DDX0700P-100] - R34-34 antigen from 125I-surface labelled Pre-Alp cells

• Reactivity: Hu, Ca
• Applications: Flow, IP, B/N
• Clone: R34-34
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IL-7R alpha/CD127 Antibody (R34-34) Summary

• Immunogen: IL7R Alpha/CD127 was used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL7R
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry 1:10-1:1000
  • Immunoprecipitation 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Dendritics and distributed by Novus Biologicals in North America and other select countries. To order in the following countries: Germany, Finland, United Kingdom, Norway, Denmark, Switzerland, Austria, Japan, and South Korea, please contact Dendritics for additional ordering information.

Alternate Names for IL-7R alpha/CD127 Antibody (R34-34)

• CD127 antigen
• CD127
• CD127ILRA
• CDW127
• IL-7 R alpha
• IL-7 receptor subunit alpha
• IL7R alpha
• IL-7R alpha
• IL-7R subunit alpha
• IL7R
• IL7RA
• IL-7Ra
• IL-7R-alpha
• interleukin 7 receptor alpha chain
• interleukin 7 receptor isoform H5-6
• interleukin 7 receptor
• interleukin-7 receptor subunit alpha

Background

Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. The human and mouse sequence is nearly identical. In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-7R alpha/CD127 Antibody (DDX0700P-100)(40)

Publications using DDX0700P-100

• Faller EM et al. IL-7 receptor recovery on CD8 T-cells isolated from HIV+ patients is inhibited by the HIV Tat protein. PLoS One 2014 Jul 17 [PMID: 25033393]
• Larbi et al. Generation of multipotent early lymphoid progenitors from human embryonic stem cells. Stem Cells Dev 2014 Dec 15 [PMID: 24955741]
• Munneke JM et al. Activated innate lymphoid cells are associated with a reduced susceptibility to graft-versus-host disease. Blood 2014 Jul 31 [PMID: 24855210]
• Forsberg A et al. GATA binding protein 3+ group 2 innate lymphoid cells are present in cord blood and in higher proportions in male than in female neonates. J Allergy Clin Immunol 2014 Jul [PMID: 24636083]
• Peguillet I et al. High numbers of differentiated effector CD4 T cells are found in patients with cancer and correlate with clinical response after neoadjuvant therapy of breast cancer. Cancer Res 2014 Apr 15 [PMID: 24535711]
• Sereti I et al. Decreases in Colonic and Systemic Inflammation inChronic HIV Infection after IL-7 Administration. PLoS Pathog 2014 Jan 30 [PMID: 24497828]
• Compeer EB et al. Tubulation of endosomal structures in human dendritic cells by Toll-like receptor ligation and lymphocyte contact accompanies antigen cross-presentation. J Biol Chem 2014 Jan 3 [PMID: 24235148]
• Yu X et al. TNF superfamily member TL1A elicits type 2 innate lymphoid cells at mucosal barriers. Mucosal Immunol 2014 May [PMID: 24220298]
• Lozza L et al. Crosstalk between human DC subsets promotes antibacterial activity and CD8+ T-cell stimulation in response to bacille Calmette-Guerin. Eur J Immunol 2014 Jan [PMID: 24114554]
• Ponchel F et al. An immunological biomarker to predict MTX response in early RA. Ann Rheum Dis 2014 Nov [PMID: 23989988]
• Bernink JH et al. Human type 1 innate lymphoid cells accumulate in inflamed mucosal tissues. Nat Immunol 2013 Mar [PMID: 23334791]
• Luckashenak N et al. Rho-family GTPase Cdc42 controls migration of Langerhans cells in vivo. J Immunol 2013 Jan 1 [PMID: 23209325]
• Ghazawi FM et al. IL-7 downregulates IL-7Ralpha expression in human CD8 T cells by two independent mechanisms. Immunol Cell Biol 2013 Feb [PMID: 23207282]
• Yatim A et al. NOTCH1 nuclear interactome reveals key regulators of its transcriptional activity and oncogenic function. Mol Cell 2012 Nov 9 [PMID: 23022380]
• Petrovas C et al. CD4 T follicular helper cell dynamics during SIV infection. J Clin Invest 2012 Sep [PMID: 22922258]
• Noakes PS et al. Increased intake of oily fish in pregnancy: effects on neonatal immune responses and on clinical outcomes in infants at 6 mo. Am J Clin Nutr 2012 Feb [PMID: 22218160]
• Young CD et al. HIV infection of thymocytes inhibits IL-7 activity without altering CD127 expression. Retrovirology 2011 Sep 16 [PMID: 21920046]
• Mjosberg JM et al. Human IL-25- and IL-33-responsive type 2 innate lymphoid cells are defined by expression of CRTH2 and CD161. Nat Immunol 2011 Sep 11 [PMID: 21909091]
• Pankewycz O et al. Low-dose rabbit antithymocyte globulin induction therapy results in prolonged selective lymphocyte depletion irrespective of maintenance immunosuppression. Transplant Proc 2011 Mar [PMID: 21440734]
• van Roon J et al. Numbers of CD25+Foxp3+ T cells that lack the IL-7 receptor are increased intra-articularly and have impaired suppressive function in RA patients. Rheumatology (Oxford) 2010 Nov [PMID: 20693259]
• Engram J et al. Lineage-specific T-cell reconstitution following in vivo CD4+ and CD8+ lymphocyte depletion in nonhuman primates. Blood 2010 Aug 5 [PMID: 20484087]
• Crawley AM et al. Soluble IL-7R alpha (sCD127) inhibits IL-7 activity and is increased in HIV infection. J Immunol 2010 May 1 [PMID: 20304824]
• Presicce P et al. Association of two clones allows for optimal detection of human FOXP3. Cytometry A 2010 Jun [PMID: 20162533]
• Valencia J et al. Transient beta-catenin stabilization modifies lineage output from human thymic CD34+CD1a- progenitors. J Leukoc Biol 2010 Mar [PMID: 19952356]
• Portevin D et al. Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy. J Transl Med 2009 Sep 24 [PMID: 19778420]
• Vudattu NK et al. Increased numbers of IL-7 receptor molecules on CD4+CD25-CD107a+ T-cells in patients with autoimmune diseases affecting the central nervous system. PLoS One 2009 Aug 6 [PMID: 19657390]
• Sauce D et al. Upregulation of interleukin 7 receptor alpha and programmed death 1 marks an epitope-specific CD8+ T-cell response that disappears following primary Epstein-Barr virus infection. J Virol 2009 Sep [PMID: 19605492]
• Luczynski W et al. Diminished expression of ICOS, GITR and CTLA-4 at the mRNA level in T regulatory cells of children with newly diagnosed type 1 diabetes. Acta Biochim Pol 2009 [PMID: 19547759]
• Faller EM et al. IL-7 and the HIV Tat protein act synergistically to down-regulate CD127 expression on CD8 T cells. Int Immunol 2009 Mar [PMID: 19147839]
• Sharma TS et al. CD8+ T-cell interleukin-7 receptor alpha expression as a potential indicator of disease status in HIV-infected children. PLoS One 2008 [PMID: 19096522]
• Mason RD et al. Low pre-infection levels and loss of central memory CD4+ T cells may predict rapid progression in SIV-infected pigtail macaques. Virology 2008 Nov 10 [PMID: 18835006]
• Johnson SE et al. IL-7 activates the phosphatidylinositol 3-kinase/AKT pathway in normal human thymocytes but not normal human B cell precursors. J Immunol 2008 Jun 15 [PMID: 18523275]
• Zonios DI et al. Idiopathic CD4+ lymphocytopenia: natural history and prognostic factors. Blood 2008 Jul 15 [PMID: 18456875]
• Esquerre M et al. Human regulatory T cells inhibit polarization of T helper cells toward antigen-presenting cells via a TGF-beta-dependent mechanism. Proc Natl Acad Sci U S A 2008 Feb 19 [PMID: 18268354]
• Esendagli G et al. Malignant and non-malignant lung tissue areas are differentially populated by natural killer cells and regulatory T cells in non-small cell lung cancer. Lung Cancer 2008 Jan [PMID: 17825949]
• Scupoli MT et al. Interleukin 7 requirement for survival of T-cell acute lymphoblastic leukemia and human thymocytes on bone marrow stroma. Haematologica 2007 Feb [PMID: 17296584]
• Hartigan-O'Connor DJ et al. Human CD4+ regulatory T cells express lower levels of the IL-7 receptor alpha chain (CD127), allowing consistent identification and sorting of live cells. J Immunol Methods 2007 Jan 30 [PMID: 17173927]
• Higgins J et al. Effects of lymphocyte isolation and timing of processing on detection of CD127 expression on T cells in human immunodeficiency virus-infected patients. Clin Diagn Lab Immunol 2005 Jan [PMID: 15643013]
• Ito T et al. Differential regulation of human blood dendritic cell subsets by IFNs. J Immunol 2001 Mar 1 [PMID: 11207245]
• Pandrau-Garcia D et al. Growth inhibitory and agonistic signals of interleukin-7 (IL-7) can be mediated through the CDw127 IL-7 receptor Blood 1994 Jun 15 [PMID: 8204885]

Bioinformatics

• Gene Symbol: IL7R