Human CD25/IL-2R alpha Quantikine ELISA Kit



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Reactivity HuSpecies Glossary
Applications ELISA

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Human CD25/IL-2R alpha Quantikine ELISA Kit Summary

The Quantikine Human IL-2 sR alpha Immunoassay is a 3.5 hour solid phase ELISA designed to measure IL-2 sR alpha in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human IL-2 sR alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-2... sR alpha showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-2 sR alpha.
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Natural and recombinant human IL-2 sR alpha
Assay Type
Solid Phase Sandwich ELISA
See PDF Datasheet for details
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details


Application Notes
No significant interference observed with available related molecules.
Read Publications using DR2A00.

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human CD25/IL-2R alpha Quantikine ELISA Kit

  • CD25 antigen
  • CD25
  • IDDM10
  • IL-2 R alpha
  • IL-2 receptor subunit alpha
  • IL2R alpha
  • IL-2R subunit alpha
  • IL2R
  • IL2RA
  • IL-2Ra
  • IL-2-RA
  • IL2-RA
  • interleukin 2 receptor, alpha
  • interleukin-2 receptor subunit alpha
  • p55
  • TAC antigen


CD25/IL-2 receptor alpha is a transmembrane protein that associates with IL-2 R beta/CD122 and the Common gamma Chain/CD132 on activated T cells and regulatory T cells (Treg). The Common gamma chain is a shared subunit in the receptors for IL-4, -7, -9, -15, and -21. IL-2 R alpha is required for mediating IL-2 induced effects including T cell proliferation, activation induced cell death (AICD) of autoreactive naive T cells, and the development of CD4+CD25+ Treg which contribute to peripheral T cell homeostasis. A soluble form of IL-2 R alpha can be generated by proteolytic cleavage of the cell surface receptor.

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for CD25/IL-2R alpha (DR2A00)(31)

We have publications tested in 3 confirmed species: Human, Mouse, Primate - Papio cynocephalus (Yellow Baboon).

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Primate - Papio cynocephalus (Yellow Baboon)
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Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using DR2A00 Applications Species
M Herrera, C Vera, Y Keynan, ZV Rueda Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review J Immunol Res, 2020;2020(0):8074183. 2020 [PMID: 32377537] (Human) Human
T Gayden, FE Sepulveda, DA Khuong-Qua, J Pratt, ET Valera, A Garrigue, S Kelso, F Sicheri, LG Mikael, N Hamel, A Bajic, R Dali, S Deshmukh, D Dervovic, D Schramek, F Guerin, M Taipale, H Nikbakht, J Majewski, D Moshous, J Charlebois, S Abish, C Bole-Feyso, P Nitschke, B Bader-Meun, D Mitchell, C Thieblemon, M Battistell, S Gravel, VH Nguyen, R Conyers, JS Diana, C McCormack, HM Prince, M Besnard, S Blanche, PG Ekert, S Fraitag, WD Foulkes, A Fischer, B Neven, D Michonneau, G de Saint B, N Jabado Germline HAVCR2 mutations altering TIM-3 characterize subcutaneous panniculitis-like T cell lymphomas with hemophagocytic lymphohistiocytic syndrome Nat. Genet., 2018;0(0):. 2018 [PMID: 30374066] (Human) Human
B Ribba, C Boetsch, TK Nayak, HP Grimm, JM Charo, S Evers, C Klein, JJL Tessier, JE Charoin, A Phipps, P Pisa, V Teichgräbe Prediction of the optimal dosing regimen using a mathematical model of tumour uptake for immunocytokine-based cancer immunotherapy Clin. Cancer Res., 2018;0(0):. 2018 [PMID: 29463551] (Human) Human
LR de Armas, S Pallikkuth, V George, S Rinaldi, R Pahwa, KL Arheart, S Pahwa Reevaluation of immune activation in the era of cART and an aging HIV-infected population JCI Insight, 2017;2(20):. 2017 [PMID: 29046481] (Human) Human
Incidence and Risk Factors for Developing Dengue-Associated Hemophagocytic Lymphohistiocytosis in Puerto Rico, 2008 - 2013 PLoS Negl Trop Dis, 2016;10(8):e0004939. 2016 [PMID: 27556807] (Human) Human
Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions Cytokine, 2016;84(0):17-24. 2016 [PMID: 27208752] (Human) Human
J Koreth, HT Kim, KT Jones, PB Lange, CG Reynolds, MJ Chammas, K Dusenbury, J Whangbo, S Nikiforow, EP Alyea, P Armand, CS Cutler, VT Ho, YB Chen, D Avigan, BR Blazar, JH Antin, J Ritz, RJ Soiffer Efficacy, durability, and response predictors of low-dose interleukin-2 therapy for chronic graft vs. host disease Blood, 2016;0(0):. 2016 [PMID: 27073224] (Human) Human
Yu P, Petrus M, Ju W, Zhang M, Conlon K, Nakagawa M, Maeda M, Bamford R, Waldmann T Augmented efficacy with the combination of blockade of the Notch-1 pathway, bortezomib and romidepsin in a murine MT-1 adult T-cell leukemia model. Leukemia, 2015;29(3):556-66. 2015 [PMID: 25118879] (Human) Human
Marzec M, Halasa K, Liu X, Wang H, Cheng M, Baldwin D, Tobias J, Schuster S, Woetmann A, Zhang Q, Turner S, Odum N, Wasik M Malignant transformation of CD4+ T lymphocytes mediated by oncogenic kinase NPM/ALK recapitulates IL-2-induced cell signaling and gene expression reprogramming. J Immunol, 2013;191(12):6200-7. 2013 [PMID: 24218456] (Human) Human
Forsvoll J, Kristoffersen E, Oymar K Elevated levels of CXCL10 in the Periodic Fever, Aphthous stomatitis, Pharyngitis and cervical Adenitis syndrome (PFAPA) during and between febrile episodes; an indication of a persistent activation of the innate immune system. Pediatr Rheumatol Online J, 2013;11(1):38. 2013 [PMID: 24134207] (Human) Human
Show All 31 Publications.

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Product General Protocols

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FAQs for CD25/IL-2R alpha (DR2A00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Gene Symbol IL2RA