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CD3 Antibody (SP7)

Images

 
Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - FFPE human tonsil stained with CD3 antibody.
Immunohistochemistry-Frozen: CD3 Antibody (SP7) [NB600-1441] - T-cells and microglia in mouse spinal cord with acute EAE. Mouse was perfused with 4% paraformaldehyde. Spinal cord was post-fixed overnight, followed by ...read more
Genetic Strategies: Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Mouse lung tissue stained against C3aR normal conditions (left) and knockdown conditions (right). IHC-P image submitted by a ...read more

Product Details

Summary
Reactivity Hu, Mu, Po, CaSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, KD
Clone
SP7
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

CD3 Antibody (SP7) Summary

Immunogen
This CD3 antibody was developed against a synthetic peptide: KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon chain.
Epitope
aa 156-168 of epsilon chain of human CD3 protein (intracytoplasmic).
Localization
Type I membrane protein.
Specificity
The CD3 antigen is present on early thymocytes and mature T cells and is generally regarded as a pan-T cell marker. This antibody will help detect CD3 expression in normal and neoplastic tissues. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CD3E
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:25-1:50
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:25-1:50
  • Knockdown Validated
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30-60 min at RT. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 29037255). Use in FLOW reported in scientific literature (PMID: 31079916).
Theoretical MW
23.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Tonsil Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 3 Reviews rated 5
using
NB600-1441 in the following applications:

Publications
Read Publications using
NB600-1441 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35111697). Canine reactivity per customer review. Use in Porcine reported in scientific literature (PMID:33839961)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tissue culture supernatant
Preservative
0.05% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CD3 Antibody (SP7)

  • CD_antigen: CD3e
  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3e
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • IMD18
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

CD3 (cluster of differentiation marker 3) is a multi-subunit transmembrane protein that is expressed on the surface of T-cells and forms a complex with the T-cell receptor (TCR) (1-3). CD3 consists of four distinct membrane protein isoforms: CD3-delta (delta), CD3-epsilon (epsilon), CD3-gamma, and CD3-zeta (1-3). The CD3 subunits organize into a complex containing three sets of dimers: CD3-epsilondelta, CD3-epsilongamma, CD3-zetazeta. The CD3 complex binds to the TCR heterodimer (alphabeta or gammadelta) to form the transmembrane TCR-CD3 complex (2-3). Structurally, the CD3 protein chains have an extracellular region, a transmembrane domain, and a cytoplasmic trail region (2-3). The CD3-epsilondelta and CD3-epsilongamma heterodimers also contain an extracellular immunoglobulin (Ig)-like domain, classifying them as part of the immunoglobulin superfamily (2-3). The cytoplasmic tail of each CD3 chain also contains one (delta, epsilon, gamma) or three (zeta) immunoreceptor tyrosine-based activation motifs (ITAMs), for a total of 10 ITAMs in the whole CD3 complex (2-3). Following, TCR binding to peptide major histocompatibility complex (p-MHC), the CD3 ITAMs are phosphorylated by the Src kinase Lck and are important for recruiting ZAP70 and initiating TCR signaling cascade activation (2). While similar in structure, the CD3 chains vary in length and molecular weight (4). The CD3-delta is 171 amino acids (aa) in length with a theoretical molecular weight of 18.9 kDa (4, 5). The CD3-epsilon is 204 aa long and has a theoretical molecular weight of 23 kDa (4, 5). CD3-gamma is 182 aa long with a theoretical molecular weight of 20.4 kDa (4, 5). Finally, CD3-zeta is 164 aa in length with a theoretical molecular weight of 18.6 kDa (4, 5).

CD3 proteins are expressed on the surface of thymocytes during thymocyte development, proliferation, and maturation to T-cells (4, 6, 7). During T-cell development CD4-CD8- double negative (DN) cells differentiate to CD4+CD8+ double positive (DP) cells before progressing to single positive (SP) CD4+ helper T-cells or CD8+ cytotoxic T-cells (4, 6, 7). As CD3 plays an important role in thymocyte development, it is understandable that CD3 defects and mutations in CD3 protein chains cause severe combined immunodeficiencies (SCIDs) (8). Additionally, a subset of CD3+ T-cells that co-express CD20 are described in a variety of diseases including rheumatoid arthritis, multiple sclerosis, CD20+ T-cell leukemia/lymphoma, and HIV (9). Clinical trials and animal models have shown that anti-CD3 monoclonal antibodies are a promising treatment modality for inflammatory disorders and autoimmune diseases, such as type I diabetes (10).

References

1. Chetty, R., & Gatter, K. (1994). CD3: structure, function, and role of immunostaining in clinical practice. The Journal of pathology. https://doi.org/10.1002/path.1711730404

2. Mariuzza, R. A., Agnihotri, P., & Orban, J. (2020). The structural basis of T-cell receptor (TCR) activation: An enduring enigma. The Journal of biological chemistry. https://doi.org/10.1074/jbc.REV119.009411

3. Kuhns, M. S., Davis, M. M., & Garcia, K. C. (2006). Deconstructing the form and function of the TCR/CD3 complex. Immunity. https://doi.org/10.1016/j.immuni.2006.01.006

4. Clevers, H., Alarcon, B., Wileman, T., & Terhorst, C. (1988). The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual review of immunology. https://doi.org/10.1146/annurev.iy.06.040188.003213

5. Uniprot: CD3-delta (P04234), CD3-epsilon (P07766), CD3-gamma (P09693), CD3-zeta (P20963)

6. D'Acquisto, F., & Crompton, T. (2011). CD3+CD4-CD8- (double negative) T cells: saviours or villains of the immune response?. Biochemical pharmacology. https://doi.org/10.1016/j.bcp.2011.05.019

7. Dave V. P. (2009). Hierarchical role of CD3 chains in thymocyte development. Immunological reviews. https://doi.org/10.1111/j.1600-065X.2009.00835.x

8. Fischer, A., de Saint Basile, G., & Le Deist, F. (2005). CD3 deficiencies. Current opinion in allergy and clinical immunology. https://doi.org/10.1097/01.all.0000191886.12645.79

9. Chen, Q., Yuan, S., Sun, H., & Peng, L. (2019). CD3+CD20+ T cells and their roles in human diseases. Human immunology. https://doi.org/10.1016/j.humimm.2019.01.001

10. Kuhn, C., & Weiner, H. L. (2016). Therapeutic anti-CD3 monoclonal antibodies: from bench to bedside. Immunotherapy. https://doi.org/10.2217/imt-2016-0049

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 Antibody (NB600-1441)(25)

We have publications tested in 3 confirmed species: Human, Mouse, Porcine.

We have publications tested in 5 applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P.


Filter By Application
Flow
(1)
ICC/IF
(9)
IHC
(7)
IHC-Fr
(2)
IHC-P
(3)
All Applications
Filter By Species
Human
(11)
Mouse
(9)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using NB600-1441 Applications Species
Pilling D, Sahlberg K, Karhadkar T et al. The sialidase NEU3 promotes pulmonary fibrosis in mice bioRxiv Feb 14 2022 (ICC/IF, Mouse) ICC/IF Mouse
Glennon E, Tongogara T, Primavera V et al. Elucidating Spatially-Resolved Changes in Host Signaling During Plasmodium Liver-Stage Infection Frontiers in Cellular and Infection Microbiology Jan 17 2022 [PMID: 35111697] (ICC/IF, Mouse) ICC/IF Mouse
Lu BY, Gupta R, Aguirre-Ducler A Et al. Spatially resolved analysis of the T cell immune contexture in lung cancer-associated brain metastases Journal for immunotherapy of cancer Oct 1 2021 [PMID: 34670827]
Miao T, Wang T, Feng T Et al. Activated invariant natural killer T cells infiltrate aortic tissue as key participants in abdominal aortic aneurysm pathology Immunology Aug 11 2021 [PMID: 34379797] (ICC/IF, Human) ICC/IF Human
Fujimoto N, He Y, D'Addio M et al. Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes PLoS Biol. Apr 1 2020 [PMID: 32251437] (IHC, Mouse) IHC Mouse
Yotis DY DM Differential Effects of KIM-1 in Subcutaneous and Orthotopic Renca Models of Kidney Cancer Thesis Jan 1 2021
Maisonneuve C, Tsang D, Foerster E Et Al. Nod1 Promotes Colorectal Carcinogenesis by Regulating the Immunosuppressive Functions of Tumor-Infiltrating Myeloid Cells SSRN Journal Jun 26 2020
Ji Y, Fan X, Zhang Y et al. Glycine regulates mucosal immunity and the intestinal microbial composition in weaned piglets Amino acids Apr 11 2021 [PMID: 33839961] (IHC, Porcine) IHC Porcine
Ingelfinger F, Krishnarajah S, Kramer M et al. Single-cell profiling of myasthenia gravis identifies a pathogenic T cell signature Acta neuropathologica Mar 28 2021 [PMID: 33774709] (IHC-Fr, Human) IHC-Fr Human
Martinez-Morilla S, Zugazagoitia J, Wong PF, et al. Quantitative analysis of CMTM6 expression in tumor microenvironment in metastatic melanoma and association with outcome on immunotherapy Oncoimmunology Dec 29 2020 [PMID: 33457084] (IHC-P, Human) IHC-P Human
Show All 25 Publications.

Reviews for CD3 Antibody (NB600-1441) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Canine and Human.

Reviews using NB600-1441:
Filter by Applications
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Canine and Human
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD3 NB600-1441
Enlarge
5
reviewed by:
Tyler Crowe
IHC-Fr Human 08/20/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman
LotJ275

Comments

CommentsHuman skin section fixed with PFA, and permeablized with 0.5% Triton. Sections were blocked with 5% BSA, before anti-CD3 ab was applied at 1:100 for 1hr at 37C. anti-rb-A568 secondary was used, and counterstained with DAPI
Immunohistochemistry-Frozen CD3 NB600-1441
Enlarge
5
reviewed by:
Xuenong Bo
IHC-Fr Mouse 02/21/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse spinal cord cryo-sections
SpeciesMouse
LotH024

Comments

CommentsMouse was perfused with 4% paraformaldehyde. Spinal cord was post-fixed overnight, followed by cryoprotection with 30% sucrose for 24h. Spinal cord sections were treated with antigen-retrieval buffer (pH 6.0) at 60C for 10 min, then cooled down to room temperature (this step should not be omitted). Anti-CD3 antibody was diluted at 1:200. Incubation: room temperature overnight.
Immunohistochemistry-Paraffin CD3 NB600-1441
Enlarge
5
reviewed by:
Hans Snyder
IHC-P Canine and Human 08/25/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Tonsil & Canine lymph paraffin slides
SpeciesCanine and Human
LotH024

Comments

CommentsWorks great in both Human and canine tissues.
Sodium citrate pH 6.0
Concentration 1:100

Product General Protocols

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Video Protocols

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FAQs for CD3 Antibody (NB600-1441) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on CD3.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

The CD4 Antibody: More than Just a Cellular Marker
CD4 is a member of the cluster of differentiation family of proteins, mainly expressed on the surface of thymocytes and a specific subset of mature T-cells. CD4 antibody studies have also shown it expressed on monocytes, cortical cells, microglial cel...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Tyler Crowe
08/20/2018
Application: IHC-Fr
Species: Human

Xuenong Bo
02/21/2018
Application: IHC-Fr
Species: Mouse

Hans Snyder
08/25/2017
Application: IHC-P
Species: Canine and Human

Bioinformatics

Gene Symbol CD3E
Entrez