CD3 Antibody (SP7)

Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Mouse lung tissue stained against C3aR normal conditions (left) and knockdown conditions (right). This image was submitted via customer review.

Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Formalin fixed paraffin embedded human tonsil stained with CD3 antibody.

Immunohistochemistry-Frozen: CD3 Antibody (SP7) [NB600-1441] - T-cells and microglia in mouse spinal cord with acute EAE. Mouse was perfused with 4% paraformaldehyde. Spinal cord was post-fixed overnight, followed by cryoprotection with 30% sucrose for 24h. Spinal cord sections were treated with antigen-retrieval buffer (pH 6.0) at 60C for 10 min, then cooled down to room temperature (this step should not be omitted). Anti-CD3 antibody was diluted at 1:200. Incubation: room temperature overnight. This image was submitted via customer review.

• Reactivity: Hu, Mu, Ca
• Applications: ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: SP7
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD3 Antibody (SP7) Summary

• Immunogen: Synthetic peptide: KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon chain.
• Epitope: aa 156-168 of epsilon chain of human CD3 protein (intracytoplasmic).
• Localization: Type I membrane protein.
• Specificity: The CD3 antigen is present on early thymocytes and mature T cells and is generally regarded as a pan-T cell marker. This antibody will help detect CD3 expression in normal and neoplastic tissues. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD3E
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC-P: Staining of formalin-fixed tissues is required by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 29037255).
• Control:

  Tonsil Lysate (NB820-59272)

Reactivity Notes

Canine reactivity per customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: 0.1% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for CD3 Antibody (SP7)

• CD3 antigen, delta subunit
• CD3
• CD3d antigen
• CD3d antigen, delta polypeptide (TiT3 complex)
• CD3d molecule, delta (CD3-TCR complex)
• CD3-DELTA
• CD3e antigen
• CD3e antigen, epsilon polypeptide (TiT3 complex)
• CD3e molecule, epsilon (CD3-TCR complex)
• CD3-epsilon
• CD3g antigen
• CD3g antigen, gamma polypeptide (TiT3 complex)
• CD3g molecule, epsilon (CD3-TCR complex)
• CD3g molecule, gamma (CD3-TCR complex)
• CD3G
• CD3-GAMMA
• FLJ17620
• FLJ17664
• FLJ18683
• FLJ79544
• FLJ94613
• MGC138597
• T3DOKT3, delta chain
• T3E
• T-cell antigen receptor complex, epsilon subunit of T3
• T-cell receptor T3 delta chain
• T-cell surface antigen T3/Leu-4 epsilon chain
• T-cell surface glycoprotein CD3 delta chain
• T-cell surface glycoprotein CD3 epsilon chain
• TCRE

Background

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD3 Antibody (NB600-1441)(7)

Publications using NB600-1441

• Hartwig T, Zwicky P, Schreiner B et al. Regulatory T Cells Restrain Pathogenic T Helper Cells during Skin Inflammation Cell Rep Dec 26 2018 [PMID: 30590032] (ICC/IF, Human)
• Toki MI, Mani N, Smithy JW et al. Immune marker profiling and PD-L1 expression across Non-Small Cell Lung Cancer mutations. J Thorac Oncol. Sep 26 2018 [PMID: 30267840] (ICC/IF, Human)
• Gushchina S, Pryce G, Yip PK et al. Increased expression of colony-stimulating factor-1 in mouse spinal cord with experimental autoimmune encephalomyelitis correlates with microglial activation and neuronal loss Glia Aug 25 2018 [PMID: 30144320] (IHC-Fr, Mouse)
• Teillaud JL, Regard L, Martin C et al. Exploring the Role of Tertiary Lymphoid Structures Using a Mouse Model of Bacteria-Infected Lungs Methods Mol. Biol. Aug 25 2018 [PMID: 30141016] (IHC-P, Mouse)
• Gettinger SN, Choi J, Mani N et al. A dormant TIL phenotype defines non-small cell lung carcinomas sensitive to immune checkpoint blockers Nat Commun Aug 10 2018 [PMID: 30097571] (IHC-P, Human)
• Carvajal-Hausdorf DE, Mani N, Velcheti V et al. Objective measurement and clinical significance of IDO1 protein in hormone receptor-positive breast cancer. J Immunother Cancer. 2017 Oct 17 [PMID: 29037255] (ICC/IF, Human)
• Brown J, Wimberly H, Lannin DR et al. Multiplexed Quantitative Analysis of CD3, CD8, and CD20 Predicts Response to Neoadjuvant Chemotherapy in Breast Cancer. Clin Cancer Res. 2014 Sep 25 [PMID: 25255793] (IHC, Human)

Reviews for CD3 Antibody (NB600-1441) (3)

Average Rating: 5

(Based on 3 reviews)

We have 3 reviews tested in 3 species: Human, Mouse, Human and Canine.

reviewed by: Tyler Crowe IHC-Fr Human 08/20/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen human skin sections
• Species: Human
• Lot: J275

Comments

• Comments: Human skin section fixed with PFA, and permeablized with 0.5% Triton. Sections were blocked with 5% BSA, before anti-CD3 ab was applied at 1:100 for 1hr at 37C. anti-rb-A568 secondary was used, and counterstained with DAPI

reviewed by: Xuenong Bo IHC-Fr Mouse 02/21/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse spinal cord cryo-sections
• Species: Mouse
• Lot: H024

Comments

• Comments: Mouse was perfused with 4% paraformaldehyde. Spinal cord was post-fixed overnight, followed by cryoprotection with 30% sucrose for 24h. Spinal cord sections were treated with antigen-retrieval buffer (pH 6.0) at 60C for 10 min, then cooled down to room temperature (this step should not be omitted). Anti-CD3 antibody was diluted at 1:200. Incubation: room temperature overnight.

reviewed by: Hans Snyder IHC-P Human and Canine 08/25/2017

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human Tonsil & Canine lymph paraffin slides
• Species: Human and Canine
• Lot: H024

Comments

• Comments: Works great in both Human and canine tissues.
  Sodium citrate pH 6.0
  Concentration 1:100

FAQs for CD3 Antibody (NB600-1441). (Showing 1 - 1 of 1 FAQs).

1. What the theoretical molecular weight for CD3 antibodies?
   • The TMW of CD3 antibodies is approximately 20 - 25 kDa.

Bioinformatics

• Gene Symbol: CD3E
• Entrez:
  • 916(Human)
  • 915(Human)
  • 917(Human)