CD3 Antibody (SP7)

Images

 
Genetic Strategies: Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Mouse lung tissue stained against C3aR normal conditions (left) and knockdown conditions (right). This image was submitted via ...read more
Immunohistochemistry-Paraffin: CD3 Antibody (SP7) [NB600-1441] - Formalin fixed paraffin embedded human tonsil stained with CD3 antibody.
Immunohistochemistry-Frozen: CD3 Antibody (SP7) [NB600-1441] - T-cells and microglia in mouse spinal cord with acute EAE. Mouse was perfused with 4% paraformaldehyde. Spinal cord was post-fixed overnight, followed by ...read more

Product Details

Summary
Reactivity Hu, Mu, CaSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clone
SP7
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
     

Genetic Strategies

   

Order Details

CD3 Antibody (SP7) Summary

Immunogen
Synthetic peptide: KAKAKPVTRGAGA, corresponding to amino acids 156-168 of Human CD3 epsilon chain.
Epitope
aa 156-168 of epsilon chain of human CD3 protein (intracytoplasmic).
Localization
Type I membrane protein.
Specificity
The CD3 antigen is present on early thymocytes and mature T cells and is generally regarded as a pan-T cell marker. This antibody will help detect CD3 expression in normal and neoplastic tissues. This antibody reacts with the intracytoplasmic portion of the CD3 antigen expressed by T cells. It stains human T cells in both the cortex and medulla of the thymus and in peripheral lymphoid tissues. This antibody is suitable for staining normal and neoplastic T cells in formalin-fixed, paraffin-embedded tissues.
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CD3E
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
IHC-P: Staining of formalin-fixed tissues is required by boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 29037255).
Control
Tonsil Lysate (NB820-59272)
Reviewed Applications
Read 3 Reviews rated 5
using
NB600-1441 in the following applications:

Publications
Read Publications using
NB600-1441 in the following applications:

Reactivity Notes

Canine reactivity per customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tissue culture supernatant
Preservative
0.1% Sodium Azide
Purity
Tissue culture supernatant

Alternate Names for CD3 Antibody (SP7)

  • CD3 antigen, delta subunit
  • CD3
  • CD3d antigen
  • CD3d antigen, delta polypeptide (TiT3 complex)
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3-DELTA
  • CD3e antigen
  • CD3e antigen, epsilon polypeptide (TiT3 complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • CD3-epsilon
  • CD3g antigen
  • CD3g antigen, gamma polypeptide (TiT3 complex)
  • CD3g molecule, epsilon (CD3-TCR complex)
  • CD3g molecule, gamma (CD3-TCR complex)
  • CD3G
  • CD3-GAMMA
  • FLJ17620
  • FLJ17664
  • FLJ18683
  • FLJ79544
  • FLJ94613
  • MGC138597
  • T3DOKT3, delta chain
  • T3E
  • T-cell antigen receptor complex, epsilon subunit of T3
  • T-cell receptor T3 delta chain
  • T-cell surface antigen T3/Leu-4 epsilon chain
  • T-cell surface glycoprotein CD3 delta chain
  • T-cell surface glycoprotein CD3 epsilon chain
  • TCRE

Background

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD3 Antibody (NB600-1441)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(2)
IHC
(1)
IHC-Fr
(1)
IHC-P
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
All Species

Reviews for CD3 Antibody (NB600-1441) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 3 species: Human, Mouse, Human and Canine.

Reviews using NB600-1441:
Filter by Applications
IHC-Fr
(2)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
Human and Canine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD3 NB600-1441
Enlarge
reviewed by:
Tyler Crowe
IHC-Fr Human 08/20/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman
LotJ275

Comments

CommentsHuman skin section fixed with PFA, and permeablized with 0.5% Triton. Sections were blocked with 5% BSA, before anti-CD3 ab was applied at 1:100 for 1hr at 37C. anti-rb-A568 secondary was used, and counterstained with DAPI
Immunohistochemistry-Frozen CD3 NB600-1441
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reviewed by:
Xuenong Bo
IHC-Fr Mouse 02/21/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedmouse spinal cord cryo-sections
SpeciesMouse
LotH024

Comments

CommentsMouse was perfused with 4% paraformaldehyde. Spinal cord was post-fixed overnight, followed by cryoprotection with 30% sucrose for 24h. Spinal cord sections were treated with antigen-retrieval buffer (pH 6.0) at 60C for 10 min, then cooled down to room temperature (this step should not be omitted). Anti-CD3 antibody was diluted at 1:200. Incubation: room temperature overnight.
Immunohistochemistry-Paraffin CD3 NB600-1441
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reviewed by:
Hans Snyder
IHC-P Human and Canine 08/25/2017
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman Tonsil & Canine lymph paraffin slides
SpeciesHuman and Canine
LotH024

Comments

CommentsWorks great in both Human and canine tissues.
Sodium citrate pH 6.0
Concentration 1:100

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for CD3 Antibody (NB600-1441). (Showing 1 - 1 of 1 FAQs).

  1. What the theoretical molecular weight for CD3 antibodies?
    • The TMW of CD3 antibodies is approximately 20 - 25 kDa.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for CD3 Antibody (NB600-1441)

Discover related pathways, diseases and genes to CD3 Antibody (NB600-1441). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for CD3 Antibody (NB600-1441)

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Pathways for CD3 Antibody (NB600-1441)

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Research Areas for CD3 Antibody (NB600-1441)

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Blogs on CD3.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

The CD4 Antibody: More than Just a Cellular Marker
CD4 is a member of the cluster of differentiation family of proteins, mainly expressed on the surface of thymocytes and a specific subset of mature T-cells. CD4 antibody studies have also shown it expressed on monocytes, cortical cells, microglial cel...  Read full blog post.

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Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

Tyler Crowe
08/20/2018
Application: IHC-Fr
Species: Human

Xuenong Bo
02/21/2018
Application: IHC-Fr
Species: Mouse

Hans Snyder
08/25/2017
Application: IHC-P
Species: Human and Canine

Bioinformatics

Gene Symbol CD3E
Entrez