CD4 Antibody - BSA Free

Images

 
Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in ...read more
Immunohistochemistry: CD4 Antibody [NBP1-19371] - DBZ inhibits the accumulation of CD4+ T cells and Th2 differentiation in the AAAs. (B) The representation of immunohistochemical staining for CD4+ in abdominal aorta ...read more
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat ...read more
Immunocytochemistry/ Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).
Flow Cytometry: CD4 Antibody - BSA Free [NBP1-19371] - An intracellular stain was performed on U-251 MG cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed ...read more
Western Blot: CD4 Antibody [NBP1-19371] - Mouse spleen, 25ug total protein. Image from verified customer review.
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Staining of paraffin-embedded mouse brain tissue. Photo courtesy of product review by verified customer.
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE tissue section of mouse brain using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody ...read more
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a ...read more
Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.
Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Simple Western: CD4 Antibody [NBP1-19371] - Lane view shows a specific band for CD4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - IFNG mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow ...read more
Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - CCR5 mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ca, RbSpecies Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

CD4 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an C-terminal region of the human CD4 Antibody protein (within residues 400-458). [Swiss-Prot P01730]
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Dual RNAscope ISH-IHC
  • Flow Cytometry 5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Simple Western 1:100
  • Western Blot 1 ug/ml
Application Notes
This CD4 antibody is useful for ICC, Western Blot, and IHC-paraffin embedded sections. In WB, a band is seen ~45 kDa. In ICC/IF, membrane staining was observed in human brain cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Jurkat cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 9 Reviews rated 4.1
using
NBP1-19371 in the following applications:

Publications
Read Publications using
NBP1-19371 in the following applications:

Reactivity Notes

Rabbit reactivity reported in the scientific literature (PMID: 22127304). Customer feedback positive on canine. Use in Porcine reported in scientific literature (PMID:33839961)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD4 Antibody - BSA Free

  • CD_antigen: CD4
  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4

Background

CD4 (cluster of differentiation 4), also known as L3T4 or T4, is a 55 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 is predominantly expressed on most thymocytes, a subset of mature T lymphocytes, and weakly on monocytes, tissue macrophages, dendritic cells, and granulocytes. It is also expressed on neurons and glial cells in the brain (1). CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells (T helper cells) further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (2). The extracellular region of CD4 consists of 372 amino acids (aa) with four immunoglobin-like domains (D1-D4). The structures of D1 and D3 resemble variable (IgV) domains while D2 and D4 resemble constant (IgC) domains (3).

Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).

References

1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C., . . . Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377

2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001

3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030

4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0

5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037

6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693

7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J., . . . Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7

8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-19371
Species: Hu, Mu, Rt, Po, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Dual ISH-IHC

Publications for CD4 Antibody (NBP1-19371)(40)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Canine, Flow, Porcine, Rabbit.

We have publications tested in 6 applications: ICC/IF, IHC, IHC-Fr, IHC-P, Rabbit, WB.


Filter By Application
ICC/IF
(8)
IHC
(12)
IHC-Fr
(2)
IHC-P
(9)
Rabbit
(1)
WB
(4)
All Applications
Filter By Species
Human
(4)
Mouse
(20)
Rat
(6)
Canine
(1)
Flow
(1)
Porcine
(1)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 40. Show All 40 Publications.
Publications using NBP1-19371 Applications Species
Dhanyalayam, D;Thangavel, H;Lizardo, K;Oswal, N;Dolgov, E;Perlin, D;Nagajyothi, J; Sex Differences in Cardiac Pathology of SARS-CoV2 Infected and Trypanosoma cruzi Co-infected Mice Frontiers in Cardiovascular Medicine [PMID: 35369283] (WB, Mouse) WB Mouse
Vieyra-Garcia P Pathophysiology of cutaneous T-cell lymphoma: Understanding of neoplastic Tcells and non-malignant infiltrate in lesional skin Thesis 2016 (ICC/IF, Human) ICC/IF Human
Borges T, Abarzua P, Gassen R et al. T cell-attracting CCL18 chemokine is a dominant rejection signal during limb transplantation Cell Reports Medicine Mar 1 2022 [PMID: 35492875] (IHC-P, Human) IHC-P Human
Alganabi M, Biouss G, Ganji N et al. Remote ischemic conditioning causes CD4 T cells shift towards reduced cell-mediated inflammation Pediatric surgery international Mar 4 2022 [PMID: 35244771]
Ohyagi M, Nagata T, Ihara K et al. DNA/RNA heteroduplex oligonucleotide technology for regulating lymphocytes in vivo Nature communications Dec 22 2021 [PMID: 34937876] (IHC-P, Mouse) IHC-P Mouse
Kolloli A, Kumar R, Singh P et al. Aggregation state of Mycobacterium tuberculosis impacts host immunity and augments pulmonary disease pathology Commun Biol Nov 3 2021 [PMID: 34732811] (Rabbit, Flow)

Details:
Citation using the Alexa Fluor 647 format of this antibody.
Rabbit Flow
Zhang C Immune Response to Age-Related and Noise-Induced Pathogenesis in the Cochlea Thesis Jan 1 2021
Balzano T, Leone P, Ivaylova G Et al. Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage Biomedicines Aug 12 2021 [PMID: 34440206] (ICC/IF, Rat) ICC/IF Rat
Ji Y, Fan X, Zhang Y et al. Glycine regulates mucosal immunity and the intestinal microbial composition in weaned piglets Amino acids Apr 11 2021 [PMID: 33839961] (IHC, Porcine) IHC Porcine
Subburaman M, Edderkaoui B Evaluation of CCL21 role in post-knee injury inflammation and early cartilage degeneration PloS one Mar 2 2021 [PMID: 33651836] (IHC-P, Rat) IHC-P Rat
Show All 40 Publications.

Reviews for CD4 Antibody (NBP1-19371) (9) 4.19

Average Rating: 4.1
(Based on 9 reviews)
We have 9 reviews tested in 4 species: Human, Mouse, Other, Sprague-Dawley rat.

Reviews using NBP1-19371:
Filter by Applications
Immunohistochemistry-Paraffin
(4)
Immunohistochemistry-Frozen
(2)
Western Blot
(2)
Flow Cytometry
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(4)
Other
(1)
Sprague-Dawley rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD4 NBP1-19371
Enlarge
5
reviewed by:
Federica Gnudi
Immunohistochemistry-Paraffin Sprague-Dawley rat 01/04/2019
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSpleen tissue
SpeciesSprague-Dawley rat
LotG1

Comments

CommentsAlcohol-fixed paraffin-embedded rat spleen tissue. Dilution: 1:150
Immunohistochemistry-Frozen CD4 NBP1-19371
Enlarge
4
reviewed by:
Mozhdeh Sojoodi
Immunohistochemistry-Frozen Mouse 06/05/2018
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedLiver
SpeciesMouse
LotNBP1-19371SS
Immunohistochemistry-Frozen CD4 NBP1-19371
Enlarge
4
reviewed by:
Verified Customer
Immunohistochemistry-Frozen Mouse 03/16/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Spleen
SpeciesMouse
Immunohistochemistry-Paraffin CD4 NBP1-19371
Enlarge
3
reviewed by:
Emily Beirne
Immunohistochemistry-Paraffin Mouse 10/06/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Spleen
SpeciesMouse
LotB-3
Immunohistochemistry-Paraffin CD4 NBP1-19371
Enlarge
4
reviewed by:
Verified Customer
Immunohistochemistry-Paraffin 10/04/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
LotA-2
Western Blot CD4 NBP1-19371
Enlarge
4
reviewed by:
Jake VanBelzen
Western Blot Other 09/19/2014
View

Summary

ApplicationWestern Blot
Sample TestedDog Spleen Homogenate
SpeciesOther
Western Blot CD4 NBP1-19371
Enlarge
5
reviewed by:
Jake VanBelzen
Western Blot 08/28/2014
View

Summary

ApplicationWestern Blot
  4
reviewed by:
Verified Customer
Flow Cytometry Human 04/24/2010
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman peripheral blood cells
SpeciesHuman
Lot360985
  4
reviewed by:
Verified Customer
Immunohistochemistry-Paraffin Mouse 04/22/2010
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Brain Tissue
SpeciesMouse
Lot360985

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD4 Antibody (NBP1-19371). (Showing 1 - 4 of 4 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. Unmasking method used for detection of Mouse CD4 cells in FFPE tissue secitons with NBP1-19371. HIER with citrate buffer pH6 or enzyme digestion. Details would be very much appreciated. I have this Ab and need some information before I begin to use it on mouse FFPE tissues.
    • I would recommend antigen retrieval using citrate buffer at pH6. Please see this link for our antigen retrieval protocol.
  3. Has this antibody ever been tested on fixed tissue without using antigen retrieval?
    • I just had a look at our QC records and I do not see any mention on using this antibody without antigen retrieval. Moreover, the inclusion or exclusion of antigen retrieval step in protocol depends primarily on the time allowed for tissues fixation (if the tissues were left in fixative for longer time, say more than 14 hours, I would not suggest you to omit the antigen retrieval step). The IHC use of our above mentioned antibody has been cited in couple of recent peer reviewed publications (Immunol Lett. 2011 Aug 30;138:169-78; Cryobiology. 2012 Feb;64:27-32) and you may follow the same for comparison of your IHC outcome.
  4. What isoform of IgG is the following antibody: NBP1-19371AF405?
    • We have only designated that it is IgG and have not isotyped it further.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on CD4. Showing 1-10 of 22 blog posts - Show all blog posts.

Synthetic Biotic Medicine as Immunotherapy Against Cancer: Evidence From Arginine-Producing Engineered Bacteria
By Jamshed Arslan, Pharm D, PhDWhat do nuts, dairy and red meat have in common? In addition to the fact that they are all edible, one of the answers is L-arginine. This amino acid improves T cell’s respons...  Read full blog post.

Immune Cell Metabolic Flux Influences Type I Diabetes
By Hunter MartinezWhat is Immunometabolism?It is well established that abnormal metabolic environments can be a risk factor for disease development. One characteristic example is the role of dyslipidemia (high lev...  Read full blog post.

Early T cell response is associated with mild COVID-19 and rapid SARS-CoV-2 clearance
Jamshed Arslan, Pharm D, PhD SARS-CoV-2 induces both humoral and cellular immunity. A vaccine or natural infection invokes SARS-CoV-2-specific humoral components (antibodies from activated B cells) and cellular resp...  Read full blog post.


  Read full blog post.


  Read full blog post.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein ...  Read full blog post.

Flow Cytometry Basics for the Non-Expert
By Jody Bonnevier, PhD.Flow cytometry: A Challenging Interdisciplinary ApplicationFlow cytometry has long been the cell analysis method of choice for immunologists, however more and more disciplines have recognize...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

Showing 1-10 of 22 blog posts - Show all blog posts.
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Recent Reviews

4.1
5
2
4
6
3
1
2
0
1
0

Federica Gnudi
01/04/2019
Application: Immunohistochemistry-Paraffin
Species: Sprague-Dawley rat

Mozhdeh Sojoodi
06/05/2018
Application: Immunohistochemistry-Frozen
Species: Mouse

Verified Customer
03/16/2017
Application: Immunohistochemistry-Frozen
Species: Mouse

Bioinformatics

Gene Symbol CD4
Entrez
Uniprot