CD4 Antibody

Images

 
Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: Lane 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) ...read more
Immunocytochemistry/ Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin ...read more
Western Blot: CD4 Antibody [NBP1-19371] - Mouse spleen, 25ug total protein. Image from verified customer review.
Western Blot: CD4 Antibody [NBP1-19371] - Dog spleen homogenate. Probed with anti-CD4 and anti-CD8. Image from verified customer review.
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Staining of paraffin-embedded mouse brain tissue. Photo courtesy of product review by verified customer.
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse brain using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin ...read more
Simple Western: CD4 Antibody [NBP1-19371] - Simple Western lane view shows a specific band for CD4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, RbSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD4 Antibody Summary

Immunogen
A synthetic peptide made to an C-terminal region of the human CD4 protein (within residues 400-458). [Swiss-Prot P01730]
Localization
Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:500
  • Immunohistochemistry-Paraffin 1:50-1:500
Application Notes
This CD4 antibody is useful for ICC, Western Blot, and IHC-paraffin embedded sections. In WB, a band is seen ~45 kDa. In ICC/IF, membrane staining was observed in human brain cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Jurkat cells.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
Kidney Lysate (NB820-59661)
Reviewed Applications
Read 6 Reviews rated 4
using
NBP1-19371 in the following applications:

Publications
Read Publications using
NBP1-19371 in the following applications:

Reactivity Notes

Rabbit reactivity reported in the scientific literature (PMID: 2212730). Customer feedback positive on canine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD4 Antibody

  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4

Background

T-cell surface glycoprotein CD4 (CD4) encodes a membrane glycoprotein found on T lymphocytes. CD4 interacts with major histocompatibility complex (MHC) class II anti-genes and is also a receptor for the human immunodeficiency virus (HIV). Once the CD4 protein binds to the cell membrane of HIV-1 gp120 and P4HB/PDI it becomes part of P4HB/PDI-CD4-CXCR4-gp120 complex. The CD4 protein interacts with HIV-1 envelope protein, Vpu, human herpes virus 7 capsid proteins, and PTK2. In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage. CD4 will also initiate or augment early phase T-cell activation. The CD4 gene is expressed in T lymphocytes, B cells, macrophages, granulocytes, and specific regions of the brain. There are three identified splice variants that encode different isoforms for CD4; one with a predicted transmembrane (TM) region and two with predicted signal peptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD4 Antibody (NBP1-19371)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rabbit.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(4)
IHC
(5)
IHC-P
(3)
WB
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(8)
Rabbit
(2)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP1-19371 Applications Species
Liu S, Pei F, Wang X et al. The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis. Oncotarget. 2017 Sep 12 [PMID: 29029533] (IHC, Rabbit) IHC Rabbit
Lin W, Wang W, Wang D, Ling W. Quercetin protects against atherosclerosis by inhibiting dendritic cell activation. Mol Nutr Food Res Apr 29 2017 [PMID: 28457022] (Mouse) Mouse
Song N, Endo D, Song B et al. 5-aza-2'-deoxycytidine impairs mouse spermatogenesis at multiple stages through different usage of DNA methyltransferases Toxicology 2016 Jun 15 [PMID: 27396502] (ICC/IF, Mouse) ICC/IF Mouse
Ren H, Li F, Tian C et al. Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice. Sci Rep. 2015 Oct 28 [PMID: 26508670] (IHC-P, Mouse) IHC-P Mouse
Gervais EM, Desantis KA, Pagendarm N et al. Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjogren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model. Anat Rec (Hoboken) 2015 Sep [PMID: 26179322] (ICC/IF) ICC/IF
Yang W, Vethanayagam RR, Dong Y et al. Activation of the antigen presentation function of mononuclear phagocyte populations associated with the basilar membrane of the cochlea after acoustic overstimulation. Neuroscience 2015 Sep 10 [PMID: 26102003] (WB) WB
Tabibian JH, O'Hara SP, Trussoni CE et al. Absence of the intestinal microbiota exacerbates hepatobiliary disease in a murine model of primary sclerosing cholangitis. Hepatology. Jan 1 2016 [PMID: 26044703] (ICC/IF, IHC, Mouse) ICC/IF, IHC Mouse
Helsby Matthew A, Leader Paul M, Fenn Joe R et al. CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited. BMC Cell Biol. 2014 Feb 14 [PMID: 24528853] (Human) Human
Zheng YH, Li FD, Tian C et al. Notch gamma-Secretase Inhibitor Dibenzazepine Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm in ApoE Knockout Mice by Multiple Mechanisms. PLoS One 2013 Dec 16 [PMID: 24358274] (IHC-P, Mouse) IHC-P Mouse
Migita K, Sho M, Shimada K et al. Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma. Cancer. 2013 Nov 18 [PMID: 24249528] (IHC, Mouse) IHC Mouse
Show All 13 Publications.

Reviews for CD4 Antibody (NBP1-19371) (6) 46

Average Rating: 4
(Based on 6 reviews)
We have 6 reviews tested in 2 species: Mouse, Other.
We have 6 reviews tested in 3 application: IHC-Fr, IHC-P, WB.

Reviews using NBP1-19371:
Filter by Applications
IHC-Fr
(1)
IHC-P
(3)
WB
(2)
All Applications
Filter by Species
Mouse
(3)
Other
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen CD4 NBP1-19371
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reviewed by:
Manuel Flores
IHC-Fr Mouse 03/16/2017
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedMouse Spleen
SpeciesMouse
Immunohistochemistry-Paraffin CD4 NBP1-19371
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reviewed by:
Anonymous
IHC-P Mouse 10/06/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Spleen
SpeciesMouse
LotB-3
Immunohistochemistry-Paraffin CD4 NBP1-19371
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reviewed by:
Merissa Olmer
IHC-P 10/04/2014
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Summary

ApplicationImmunohistochemistry-Paraffin
LotA-2
CommentsThese tissue samples were fixed with zinc buffered formalin.
Western Blot CD4 NBP1-19371
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reviewed by:
Anonymous
WB Other 09/19/2014
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Summary

ApplicationWestern Blot
Sample TestedDog Spleen Homogenate
SpeciesOther
CommentsMembrane was dual stained with primary antibodies to CD4 (detailed here) and CD8 alpha chain (also raised in rabbit, not detailed here). I included a long term exposure time to show non-specific bands. Bands of interest are present at ~54 kDa (CD4) and ~26kDa (CD8 alpha chain).

Blocking

Blocking DetailsTBS, 0.1% Tween, 5% Milk; 1 hour at 4C

Primary Anitbody

Dilution Ratio0.1ug/ul in TBS, 0.1% Tween, 5% Milk; 15 hours at 4C

Secondary Antibody

Secondary Descriptiondonkey anti-rabbit IgG-HRP
Secondary Manufacturer Cat#sc-2077
Secondary Concentration1:5000

Details

Detection NotesECL; exposed for 5min and 15 hours

Comments

CommentsMembrane was dual stained with primary antibodies to CD4 (detailed here) and CD8 alpha chain (also raised in rabbit, not detailed here). I included a long term exposure time to show non-specific bands. Bands of interest are present at ~54 kDa (CD4) and ~26kDa (CD8 alpha chain).
Western Blot CD4 NBP1-19371
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reviewed by:
Anonymous
WB 08/28/2014
View

Summary

ApplicationWestern Blot
CommentsThis was my first time using this antibody - nothing like it working right off the bat! However, I used 0.5ug/mL primary with 25ug spleen protein, and only had to expose my film for 30 seconds. I'm going to try 0.1, possibly 0.01 ug/mL next time.
Immunohistochemistry-Paraffin CD4 NBP1-19371
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reviewed by:
Rahul Mittal
IHC-P Mouse 04/22/2010
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Brain Tissue
SpeciesMouse
Lot360985

Details

Detection NotesAntigen Retrieval: High Ph Antigen retrieval solution, 20 minutes at 95 degrees Celsius
Fixation DetailsSample Preparation: Paraffin embedded

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD4 Antibody (NBP1-19371). (Showing 1 - 3 of 3 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
  2. Unmasking method used for detection of Mouse CD4 cells in FFPE tissue secitons with NBP1-19371. HIER with citrate buffer pH6 or enzyme digestion. Details would be very much appreciated. I have this Ab and need some information before I begin to use it on mouse FFPE tissues.
    • I would recommend antigen retrieval using citrate buffer at pH6. Please see this link for our antigen retrieval protocol.
  3. Has this antibody ever been tested on fixed tissue without using antigen retrieval?
    • I just had a look at our QC records and I do not see any mention on using this antibody without antigen retrieval. Moreover, the inclusion or exclusion of antigen retrieval step in protocol depends primarily on the time allowed for tissues fixation (if the tissues were left in fixative for longer time, say more than 14 hours, I would not suggest you to omit the antigen retrieval step). The IHC use of our above mentioned antibody has been cited in couple of recent peer reviewed publications (Immunol Lett. 2011 Aug 30;138:169-78; Cryobiology. 2012 Feb;64:27-32) and you may follow the same for comparison of your IHC outcome.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on CD4. Showing 1-10 of 14 blog posts - Show all blog posts.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Lymphocyte activation gene 3 (LAG3): No lag time in immune response
The LAG3 protein belongs to the Ig superfamily and contains 4 extracellular Ig-like domains (D1-D4). This molecule plays an key role in the immune response through negative regulation of T-cell proliferation, function, and homeostasis. It is required...  Read full blog post.

CD4 - An Important Co-receptor Assisting TCRs
The cluster of differentiation 4 (CD4) protein is a surface, type I membrane-embedded glycoprotein that is found on a wide range of cells: T-lymphocytes, B-cells, macrophages, granulocytes, and developmentally-dependent regions specific to the brain....  Read full blog post.

CD4, HIV and T Cell Signaling
CD4, also known as Cluster of Differentiation 4, interacts with major histocompatibility complex class II antigens, acts as a receptor for the human immunodeficiency virus and induces the aggregation of lipid rafts. It is expressed in T lymphocytes, B...  Read full blog post.

Showing 1-10 of 14 blog posts - Show all blog posts.

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Recent Reviews

4
5
1
4
4
3
1
2
0
1
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Manuel Flores
03/16/2017
Application: IHC-Fr
Species: Mouse

Anonymous
10/06/2015
Application: IHC-P
Species: Mouse

Merissa Olmer
10/04/2014
Application: IHC-P
Species:

Bioinformatics

Gene Symbol CD4
Entrez
Uniprot