CD4 Antibody - BSA Free

Immunohistochemistry: CD4 Antibody [NBP1-19371] - Increased CD3+ and CD4+ T-cell occurrence in the brainstem of SHR-72 transgenic rat model for tauopathies. (A-D) Immunofluorescence staining showed CD4+ T-cells in SHR-72 transgenic animals. (E- H) Immunofluorescence staining showed more perivascular than brain parenchyma infiltrating CD4+ T-cells in SHR-72 transgenic animals. PLoS One. 2019 May 23;14(5):e0217216. doi: 10.1371/journal.pone.0217216.

Immunohistochemistry: CD4 Antibody [NBP1-19371] - DBZ inhibits the accumulation of CD4+ T cells and Th2 differentiation in the AAAs. (B) The representation of immunohistochemical staining for CD4+ in abdominal aorta from four groups (left). Bar graphs show the percentage of CD4+ positive cell areas (right; n=3 per group). Bar: 50 um.

Western Blot: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in the following cell lysates: 1) human spleen 2) human tonsil 3) human placenta 4) human kidney 5) human liver 6) mouse spleen 7) mouse placenta 8) rat placenta.

Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP1-19371] - CD4 antibody was tested in Jurkat cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).

Flow Cytometry: CD4 Antibody - BSA Free [NBP1-19371] - An intracellular stain was performed on U-251 MG cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Western Blot: CD4 Antibody [NBP1-19371] - Mouse spleen, 25ug total protein. Image from verified customer review.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Staining of paraffin-embedded mouse brain tissue. Photo courtesy of product review by verified customer.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE tissue section of mouse brain using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody primarily generated a strong signal in the membranes of a subset of cells in the tested section.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Analysis of a FFPE section of mouse spleen using CD4 antibody at 1:300 dilution with HRP-DAB detection and hematoxylin counterstaining. The antibody generated a strong signal in the membranes of a subset of cells in the spleen section and the staining showed punctate appearance in some cells.

Immunohistochemistry-Frozen: CD4 Antibody [NBP1-19371] - Staining in mouse liver tissue.

Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-19371] - Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution 1:150. Image from verified customer review.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP1-19371AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371AF594 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 594.

Flow Cytometry: CD4 Antibody [NBP1-19371] - An intracellular stain was performed on U937 cells with CD4 Antibody NBP1-19371G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.

Simple Western: CD4 Antibody [NBP1-19371] - Lane view shows a specific band for CD4 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - IFNG mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-IFNG and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin (Cat. No. DS9800), BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

Dual RNAscope ISH-IHC: CD4 Antibody [NBP1-19371] - CCR5 mRNA (red) and CD4 protein (green) were detected in formalin-fixed paraffin-embedded tissue sections of human lung cancer. ACD's Integrated Co-Detection Workflow was performed using ACD RNAScope Probe Hs-CCR5 and CD4 antibody at 1:100 dilution. Tissue was stained on Leica Bond RX using RNAscope (TM) 2.5 LS Reagent Kit-RED, BOND Polymer Refine Detection (DAB) and Hematoxylin, BOND Polymer Refine Red Detection and Hematoxylin and RNAscope (TM) 2.5 LS Green Accessory Pack. Tissue was counterstained with 50% hematoxylin (blue).

• Reactivity: Hu, Mu, Rt, Po, Ca, Rb
• Applications: WB, Simple Western, Flow, ICC/IF, IHC, Dual ISH-IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD4 Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an C-terminal region of the human CD4 Antibody protein (within residues 400-458). [Swiss-Prot P01730]
• Localization: Membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CD4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC
  • Flow Cytometry 5 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:500
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1:50-1:500
  • Simple Western 1:100
  • Western Blot 1 ug/ml
• Application Notes: This CD4 antibody is useful for ICC, Western Blot, and IHC-paraffin embedded sections. In WB, a band is seen ~45 kDa. In ICC/IF, membrane staining was observed in human brain cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. In ICC/IF, membrane staining was observed in Jurkat cells.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
• Control:

  Mouse Kidney Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Rabbit reactivity reported in the scientific literature (PMID: 22127304). Customer feedback positive on canine. Use in Porcine reported in scientific literature (PMID:33839961)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CD4 Antibody - BSA Free

• CD_antigen: CD4
• CD4 antigen (p55)
• CD4 antigen
• CD4 molecule
• CD4 receptor
• CD4
• CD4mut
• T-cell surface antigen T4/Leu-3
• T-cell surface glycoprotein CD4

Background

CD4 (cluster of differentiation 4), also known as L3T4 or T4, is a 55 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 is predominantly expressed on most thymocytes, a subset of mature T lymphocytes, and weakly on monocytes, tissue macrophages, dendritic cells, and granulocytes. It is also expressed on neurons and glial cells in the brain (1). CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells (T helper cells) further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (2). The extracellular region of CD4 consists of 372 amino acids (aa) with four immunoglobin-like domains (D1-D4). The structures of D1 and D3 resemble variable (IgV) domains while D2 and D4 resemble constant (IgC) domains (3).

Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).

References

1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C., . . . Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377

2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001

3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030

4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0

5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037

6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693

7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J., . . . Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7

8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD4 Antibody (NBP1-19371)(45)

Publications using NBP1-19371

• Son S, Nam J, Kim AS et al. Induction of T-helper-17-cell-mediated anti-tumour immunity by pathogen-mimicking polymer nanoparticles Nature biomedical engineering 2022-12-23 [PMID: 36564626] (IHC-Fr, Mouse)
• Yuan M, Qian X, Huang Y et al. Th17 Activation and Th17/Treg Imbalance in Prolonged Anterior Intraocular Inflammation after Ocular Alkali Burn International journal of molecular sciences 2022-06-25 [PMID: 35806082]
• Borges T, Abarzua P, Gassen R et al. T cell-attracting CCL18 chemokine is a dominant rejection signal during limb transplantation Cell Reports Medicine 2022-03-01 [PMID: 35492875] (IHC-P, Human)
• Dhanyalayam, D;Thangavel, H;Lizardo, K;Oswal, N;Dolgov, E;Perlin, D;Nagajyothi, J; Sex Differences in Cardiac Pathology of SARS-CoV2 Infected and Trypanosoma cruzi Co-infected Mice Frontiers in Cardiovascular Medicine [PMID: 35369283] (WB, Mouse)
• Alganabi M, Biouss G, Ganji N et al. Remote ischemic conditioning causes CD4 T cells shift towards reduced cell-mediated inflammation Pediatric surgery international 2022-03-04 [PMID: 35244771]
• Ohyagi M, Nagata T, Ihara K et al. DNA/RNA heteroduplex oligonucleotide technology for regulating lymphocytes in vivo Nature communications 2021-12-22 [PMID: 34937876] (IHC-P, Mouse)
• Kolloli A, Kumar R, Singh P et al. Aggregation state of Mycobacterium tuberculosis impacts host immunity and augments pulmonary disease pathology Commun Biol 2021-11-03 [PMID: 34732811] (Rabbit, Flow)
  
  Details:
  Citation using the Alexa Fluor 647 format of this antibody.
• Balzano T, Leone P, Ivaylova G Et al. Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage Biomedicines 2021-08-12 [PMID: 34440206] (ICC/IF, Rat)
• Ji Y, Fan X, Zhang Y et al. Glycine regulates mucosal immunity and the intestinal microbial composition in weaned piglets Amino acids 2021-04-11 [PMID: 33839961] (IF/IHC, Porcine)
• Subburaman M, Edderkaoui B Evaluation of CCL21 role in post-knee injury inflammation and early cartilage degeneration PloS one 2021-03-02 [PMID: 33651836] (IHC-P, Rat)
• Mondal T, Shivange GN, Tihagam RG et al. Unexpected PD-L1 immune evasion mechanism in TNBC, ovarian, and other solid tumors by DR5 agonist antibodies EMBO molecular medicine 2021-02-15 [PMID: 33587338]
• Li H, Chen Y, Jin Q, et al. Noninvasive Radionuclide Molecular Imaging of the CD4-Positive T Lymphocytes in Acute Cardiac Rejection Molecular pharmaceutics 2021-01-28 [PMID: 33506680] (WB)
• Herath KHINM, Kim HJ, Kim A et al. The Role of Fucoidans Isolated from the Sporophylls of Undaria pinnatifida against Particulate-Matter-Induced Allergic Airway Inflammation: Evidence of the Attenuation of Oxidative Stress and Inflammatory Responses Molecules 2020-06-22 [PMID: 32580518] (IHC-P, Mouse)
• Zhang C, Frye M, Sun W et al. New insights on repeated acoustic injury: Augmentation of cochlear susceptibility and inflammatory reaction resultant of prior acoustic injury Hearing Research 2020-01-01 [PMID: 32534268] (IF/IHC, Mouse)
• Song KH, Jung SY, Park JI et al. Evaluation of Anti-Tumor Effects of Whole-Body Low-Dose Irradiation in Metastatic Mouse Models Cancers (Basel) 2020-04-30 [PMID: 32365904] (ICC/IF, Mouse)
• Partridge BR, O'Brien TJ, Lorenzo MF et al. High-Frequency Irreversible Electroporation for Treatment of Primary Liver Cancer: A Proof-of-Principle Study in Canine Hepatocellular Carcinoma J Vasc Interv Radiol 2020-01-16 [PMID: 31956003] (Canine)
• Wang W, Cohen JA, Wallrapp A et al. Age-Related Dopaminergic Innervation Augments T Helper 2-Type Allergic Inflammation in the Postnatal Lung Immunity 2019-11-18 [PMID: 31757673] (Mouse)
• Bishehsari F, Engen P A et al. Abnormal Eating Patterns Cause Circadian Disruption and Promote Alcohol-Associated Colon Carcinogenesis. Cell Mol Gastroenterol Hepatol 2019-02-11 [PMID: 31689559] (IF/IHC, Mouse)
• Hong SK, Choo EH, Ihm SH, Chang KY High fat diet downregulates regulatory T cells in the myocardium of spontaneous hypertensive rats Nutr Metab Cardiovasc Dis 2019-11-01 [PMID: 31585776] (IHC-Fr, Rat)
• Jung KJ, Lee GW, Park CH et al. Mesenchymal Stem Cells Decrease Oxidative Stress in the Bowels of Interleukin-10 Knockout Mice Gut Liver 2020-01-15 [PMID: 31158947] (WB, Mouse)
• Ji H, Zheng W et al. Sex-specific T-cell regulation of angiotensin II-dependent hypertension. Hypertension 2014-01-09 [PMID: 24935938] (IF/IHC, Mouse)
• Helsby Matthew A, Leader Paul M, Fenn Joe R et al. CiteAb: a searchable antibody database that ranks antibodies by the number of times they have been cited. BMC Cell Biol. 2014-02-14 [PMID: 24528853] (Human)
• Vieyra-Garcia P Pathophysiology of cutaneous T-cell lymphoma: Understanding of neoplastic Tcells and non-malignant infiltrate in lesional skin Thesis 2016-01-01 (ICC/IF, Human)
• Nedrelow DS, Rassi A, Ajeeb B et al. Regenerative Engineering of a Biphasic Patient-Fitted Temporomandibular Joint Condylar Prosthesis Tissue engineering. Part C, Methods 2023-06-19 [PMID: 37335050]
• Zhou X, Zhang X, Niu D et al. Gut Microbiota Induces Hepatic Steatosis by Modulating the T Cells Balance in High Fructose Diet Mice Research Square 2023-01-04 [PMID: 37095192]
• Oswal N, Thangavel H, Lizardo K et al. Diets Differently Regulate Pulmonary Pathogenesis and Immune Signaling in Mice during Acute and Chronic Mycobacterium tuberculosis Infection Life (Basel, Switzerland) 2023-01-13 [PMID: 36676177] (IHC-P, Mouse)
• Wu Y, Qin YH, Liu Y et al. Cardiac troponin I autoantibody induces myocardial dysfunction by PTEN signaling activation EBioMedicine 2019-08-29 [PMID: 31474552] (IHC-P, Human, Mouse)
• Zhang C Immune Response to Age-Related and Noise-Induced Pathogenesis in the Cochlea Int J Mol Sci 2019-06-21 [PMID: 31216722]
• De Biase D, Esposito F, De Martino M et al. Characterization of inflammatory infiltrate of ulcerative dermatitis in C57BL/6NCrl-Tg(HMGA1P6)1Pg mice Lab. Anim. 2018-12-06 [PMID: 30522404] (IF/IHC, Mouse)
• Lee MS, Lin HS, Wallace CG, Sheu JJ. Impact of Combined Adipose-Derived Mesenchymal Stem Cell (ADMSC) and Low-Energy of Extracorporeal Shock Wave Therapy on Protecting Brain Death-Induced Remote Organ Damage in Rat Neuropsychiatry (IF/IHC, ICC/IF, Rat)
• Majerova Petra, Michalicova Alena, Cente Martin et al. Trafficking of immune cells across the blood-brain barrier is modulated by neurofibrillary pathology in tauopathies. PloS One 2019-05-23 [PMID: 31120951] (IHC-Fr, Rat)
• Liu S, Pei F, Wang X et al. The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis. Oncotarget. 2017-09-12 [PMID: 29029533] (IF/IHC, Rabbit)
• Friedman O, Carmel N, Sela M et al. Immunological and inflammatory mapping of vascularized composite allograft rejection processes in a rat model PLoS ONE 2017-07-26 [PMID: 28746417] (IHC-P, Rat)
• Lin W, Wang W, Wang D, Ling W. Quercetin protects against atherosclerosis by inhibiting dendritic cell activation. Mol Nutr Food Res 2017-04-29 [PMID: 28457022] (Mouse)
• Kim So Young, Kang Dongxu, Choi Hye Jin et al. Prime-boost immunization by both DNA vaccine and oncolytic adenovirus expressing GM-CSF and shRNA of TGF-b2 induces anti-tumor immune activation. Oncotarget 2017-01-01 [PMID: 28178658] (IF/IHC, Mouse)
• Song N, Endo D, Song B et al. 5-aza-2'-deoxycytidine impairs mouse spermatogenesis at multiple stages through different usage of DNA methyltransferases Toxicology 2016-06-15 [PMID: 27396502] (ICC/IF, Mouse)
• Ren H, Li F, Tian C et al. Inhibition of Proteasome Activity by Low-dose Bortezomib Attenuates Angiotensin II-induced Abdominal Aortic Aneurysm in Apo E(-/-) Mice. Sci Rep. 2015-10-28 [PMID: 26508670] (IHC-P, Mouse)
• Gervais EM, Desantis KA, Pagendarm N et al. Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjogren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model. Anat Rec (Hoboken) 2015-09-01 [PMID: 26179322] (ICC/IF)
• Yang W, Vethanayagam RR, Dong Y et al. Activation of the antigen presentation function of mononuclear phagocyte populations associated with the basilar membrane of the cochlea after acoustic overstimulation. Neuroscience 2015-09-10 [PMID: 26102003] (WB)
• Tabibian JH, O'Hara SP, Trussoni CE et al. Absence of the intestinal microbiota exacerbates hepatobiliary disease in a murine model of primary sclerosing cholangitis. Hepatology. 2016-01-01 [PMID: 26044703] (ICC/IF, IF/IHC, Mouse)
• Zheng YH, Li FD, Tian C et al. Notch gamma-Secretase Inhibitor Dibenzazepine Attenuates Angiotensin II-Induced Abdominal Aortic Aneurysm in ApoE Knockout Mice by Multiple Mechanisms. PLoS One 2013-12-16 [PMID: 24358274] (IHC-P, Mouse)
• Migita K, Sho M, Shimada K et al. Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma. Cancer. 2013-11-18 [PMID: 24249528] (IF/IHC, Mouse)
• Yasuda S, Sho M, Yamato I et al. Simultaneous blockade of programmed death 1 and vascular endothelial growth factor receptor 2 (VEGFR2) induces synergistic anti-tumour effect in vivo. Clin Exp Immunol. 2013-06-01 [PMID: 23600839] (IHC-P, Mouse)
• Lu Y, Li B, Wang X et al. The effect of programmed cryopreservation on immunogenicity of bladder mucosa in New Zealand rabbits. Cryobiology. 2011-11-22 [PMID: 22127304] (IF/IHC, Rabbit)
• Hwang I, Ahn G, Park E et al. An acidic polysaccharide of Panax ginseng ameliorates experimental autoimmune encephalomyelitis and induces regulatory T cells Immunol Lett 2011-08-30 [PMID: 21524666] (ICC/IF, IF/IHC, Mouse)

Reviews for CD4 Antibody (NBP1-19371) (9)

Average Rating: 4.1

(Based on 9 reviews)

We have 9 reviews tested in 4 species: Human, Mouse, Other, Sprague-Dawley rat.

reviewed by: Federica Gnudi IHC-P Sprague-Dawley rat 01/04/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Spleen tissue
• Species: Sprague-Dawley rat
• Lot: G1

Comments

• Comments: Alcohol-fixed paraffin-embedded rat spleen tissue. Dilution: 1:150

reviewed by: Mozhdeh Sojoodi IHC-Fr Mouse 06/05/2018

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Liver
• Species: Mouse
• Lot: NBP1-19371SS

reviewed by: Verified Customer IHC-Fr Mouse 03/16/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse Spleen
• Species: Mouse

reviewed by: Emily Beirne IHC-P Mouse 10/06/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Spleen
• Species: Mouse
• Lot: B-3

reviewed by: Verified Customer IHC-P 10/04/2014

Summary

• Application: Immunohistochemistry-Paraffin
• Lot: A-2

reviewed by: Jake VanBelzen WB Other 09/19/2014

Summary

• Application: Western Blot
• Sample Tested: Dog Spleen Homogenate
• Species: Other

reviewed by: Jake VanBelzen WB 08/28/2014

Summary

• Application: Western Blot

reviewed by: Verified Customer Flow Human 04/24/2010

Summary

• Application: Flow Cytometry
• Sample Tested: Human peripheral blood cells
• Species: Human
• Lot: 360985

reviewed by: Verified Customer IHC-P Mouse 04/22/2010

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Mouse Brain Tissue
• Species: Mouse
• Lot: 360985

FAQs for CD4 Antibody (NBP1-19371). (Showing 1 - 4 of 4 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. Unmasking method used for detection of Mouse CD4 cells in FFPE tissue secitons with NBP1-19371. HIER with citrate buffer pH6 or enzyme digestion. Details would be very much appreciated. I have this Ab and need some information before I begin to use it on mouse FFPE tissues.
   • I would recommend antigen retrieval using citrate buffer at pH6. Please see this link for our antigen retrieval protocol.
3. Has this antibody ever been tested on fixed tissue without using antigen retrieval?
   • I just had a look at our QC records and I do not see any mention on using this antibody without antigen retrieval. Moreover, the inclusion or exclusion of antigen retrieval step in protocol depends primarily on the time allowed for tissues fixation (if the tissues were left in fixative for longer time, say more than 14 hours, I would not suggest you to omit the antigen retrieval step). The IHC use of our above mentioned antibody has been cited in couple of recent peer reviewed publications (Immunol Lett. 2011 Aug 30;138:169-78; Cryobiology. 2012 Feb;64:27-32) and you may follow the same for comparison of your IHC outcome.
4. What isoform of IgG is the following antibody: NBP1-19371AF405?
   • We have only designated that it is IgG and have not isotyped it further.

Bioinformatics

• Gene Symbol: CD4
• Entrez:
  • 920(Human)
  • 12504(Mouse)
• Uniprot:
  • P01730()
  • P06332()