CD8 Antibody (53-6.7)

Images

 
Immunocytochemistry/ Immunofluorescence: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room ...read more
Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
Immunocytochemistry/ Immunofluorescence: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.
Immunocytochemistry/ Immunofluorescence: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of splenocytes. Primary antibody was used at a dilution of 10 ug/mL and incubated for 3 hours at room temperature.
Immunohistochemistry-Paraffin: CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer review.
Immunohistochemistry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of frozen spleen sections. Blocking: 1 hour at 25C with serum at 5% concentration.
Immunohistochemistry-Frozen: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes were stained with biotinylated CD8 (clone 53-6.7) (filled histogram) or rat IgG2a, k isotype control (open histogram), followed by ...read more
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - C57BL/6 mouse splenocytes were stained with purified CD8 (clone 53-6.7) (filled histogram) or rat IgG2a, k isotype control (open histogram), followed by ...read more
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine splenocytes by multiple staining.
Flow Cytometry: CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blood leukocytes.

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, Func
Clone
53-6.7
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

CD8 Antibody (53-6.7) Summary

Description
This CD8 alpha antibody serves as an effective marker of cytotoxic T lymphocytes by binding to the CD8 co-receptor expressed on the cell surface of cytotoxic T cells, recognizing the topological domain of CD8 alpha. Because this CD8 antibody is made to the alpha chain it will recognize both the CD8 alpha - CD8 beta heterodimer, the most common form, as well as the CD8 alpha - CD8 alpha homodimer. The CD8A gene is also expressed in natural killer cells (NK cells), dendritic cells and cortical thymocytes making the CD8 alpha antibody a potential marker for these cells.
Immunogen
Mouse thymus or spleen.
Localization
Most thymocytes, T cell subset, some NK cells
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
CD8A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Cell depletion
  • CyTOF-ready
  • Inhibition of T Cell Function
Application Notes
Each lot of this CD8a antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is <0.25 ug/10^6 cells in 100 uL volume. It is recommended that the reagent be titrated for optimal performance for each application. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of both acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Use in ICC/IF was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24565643).
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-49045 in the following applications:

Publications
Read Publications using
NBP1-49045 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for CD8 Antibody (53-6.7)

  • 53-6.7 CD8 Alpha
  • 53-6.7 CD8 Aplha
  • 53-6.7 CD8
  • 53-6.7 Clone
  • 53-6.7
  • CD8 Antibody
  • CD8
  • CD8a molecule
  • CD8A
  • Cytotoxic T cell marker
  • Cytotoxic T lymphocyte marker
  • LEU2
  • p32
  • T cell marker

Background

CD8, also known as MAL, p32, Leu2 and T8-T cell antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a 34kD protein that belongs to the immunoglobulin family and consists of disulfide-linked alpha and beta chains that form the alpha (CD8a)/beta (CD8b) heterodimer and alpha/alpha homodimer. The CD8 alpha/beta heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR alpha/beta T cells. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. Multiple transcript variants encoding different isoforms have been found for this gene. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Studies from Cambridge with the CD8 alpha antibody have shown 2 different types of tolerance (permanent or reversible) are induced in peripheral T-cells depending on the type of antigen they are exposed to(1). Associated studies with the same subjects used the CD8 alpha antibody to show that a simple monoclonal CD4 and CD8 monoclonal antibody therapy can establish transplantation tolerance(2). Another study in Takahashi's lab involving CD4 and CD8 used the CD8 alpha antibody in order to map signal molecules implicated in mediating the effects of interleukin 2 on T-cells(3).
PMIDs:
1. 1702726
2. 1980112
3. 1608966

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD8 alpha Antibody (NBP1-49045)(12)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 5 applications: FLOW, Flow, ICC/IF, IHC, IHC-P.


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FLOW
(1)
Flow
(1)
ICC/IF
(3)
IHC
(3)
IHC-P
(1)
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Mouse
(9)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NBP1-49045 Applications Species
Fazia T, Pastorino R, Notartomaso S et al. Acid Sensing Ion Channel 2: a new potential player in the pathophysiology of multiple sclerosis Eur. J. Neurosci. Dec 14 2018 [PMID: 30549327] (IHC, Mouse) IHC Mouse
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (Flow, Mouse)

Details:
This citation used the PE form of this antbody
Flow Mouse
Sierra RA, Trillo-Tinoco J, Mohamed E et al. Anti-Jagged immunotherapy inhibits MDSCs and overcomes tumor-induced tolerance Cancer Res. 2017 Sep 13 [PMID: 28904063] (ICC/IF, Mouse) ICC/IF Mouse
Ma S, Deng W, Liu J et al. Blockade of adenosine A2A receptor enhances CD8+ T cells response and decreases regulatory T cells in head and neck squamous cell carcinoma. Mol Cancer 2017 Jun 07 [PMID: 28592285] (IHC, Mouse) IHC Mouse
Cohen N, Shani O, Raz Y et al. Fibroblasts drive an immunosuppressive and growth-promoting microenvironment in breast cancer via secretion of Chitinase 3-like 1. Oncogene. Apr 3 2017 [PMID: 28368410] (ICC/IF, Mouse) ICC/IF Mouse
Li A, Wu Y, Tang F et al. In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice. Cancer Biother. Radiopharm. Nov 1 2016 [PMID: 27831762] (Mouse) Mouse
Fazio F, Zappulla C, Notartomaso S et al. Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice. Neuropharmacology 2014 Mar 3 [PMID: 24565643] (IHC-P, Mouse) IHC-P Mouse
KheradmT, Wang S, Gibly R et al. Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions Biomaterials 2011 Jul [PMID: 21458857] (IHC, ICC/IF, Mouse) IHC, ICC/IF Mouse
Bankoti J, Burnett A, Navarro S et al. Effects of TCDD on the fate of naive dendritic cells. Toxicol Sci. 2010 Jun. [PMID: 20211938]
McLellan AD, Kapp M, Eggert A et al. Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression. Blood. [PMID: 11877283] (FLOW, Mouse) FLOW Mouse
Show All 12 Publications.

Reviews for CD8 alpha Antibody (NBP1-49045) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-49045:
Filter by Applications
IHC-P
(2)
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Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD8 NBP1-49045
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reviewed by:
Anonymous
IHC-P Mouse 10/23/2017
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse Head and neck squamous cell carcinoma (HNSCC)
SpeciesMouse
LotNBP1-49045

Comments

Commentssections were incubated with antibody for CD8 alpha (Novus, 1:200, for mouse HNSCC samples) at 4 °C for 12 h.
Immunohistochemistry-Paraffin CD8 NBP1-49045
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reviewed by:
Naiche Adler
IHC-P Mouse 12/15/2014
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedMouse Spleen
SpeciesMouse

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FAQs for CD8 alpha Antibody (NBP1-49045) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-49045AF405
Alexa Fluor 488 Labeled NBP1-49045AF488
Alexa Fluor 647 Labeled NBP1-49045AF647
Alexa Fluor 700 Labeled NBP1-49045AF700
Allophycocyanin Labeled NBP1-49045APC
Biotin Labeled NBP1-49045B
DyLight 350 Labeled NBP1-49045UV
DyLight 405 Labeled NBP1-49045V
DyLight 488 Labeled NBP1-49045G
DyLight 550 Labeled NBP1-49045R
DyLight 650 Labeled NBP1-49045C
DyLight 680 Labeled NBP1-49045FR
DyLight 755 Labeled NBP1-49045IR
PE Labeled NBP1-49045PE
PerCP Labeled NBP1-49045PCP

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Blogs on CD8.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

The CD4 Antibody: More than Just a Cellular Marker
CD4 is a member of the cluster of differentiation family of proteins, mainly expressed on the surface of thymocytes and a specific subset of mature T-cells. CD4 antibody studies have also shown it expressed on monocytes, cortical cells, microglial cel...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
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Anonymous
10/23/2017
Application: IHC-P
Species: Mouse

Naiche Adler
12/15/2014
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol CD8A