CD8 Antibody (53-6.7)


Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of lymph nodes by multiple staining.
Immunohistochemistry-Paraffin: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - CD8 alpha expression in mouse spleen tissue using anti-CD8 alpha antibody. Image from verified customer more
Immunocytochemistry/ Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of bone marrow tissue by single and multiple staining.
Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Staining of peripheral blood leukocytes.
Immunocytochemistry/ Immunofluorescence: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of immersion fixed splenocytes. Primary antibody was used at a dilution of 10 ug/mL and more
Immunohistochemistry: CD8 Antibody (53-6.7) [NBP1-49045] - Histopathological analysis of spinal cord in EAE mice. Representative spinal cord sections from wild-type (WT), Asic1-/-, and Asic2-/- mice at 45 days after more
Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - CD8 alpha Antibody (53-6.7) [NBP1-49045] - Analysis of mosue spleen sections. CD8+ lymphocytes are marked by brown labeling of the cell surface.
Immunohistochemistry-Frozen: CD8 Antibody (53-6.7) [NBP1-49045] - Analysis of CD8+ T cells in allogeneic skin grafted onto a mouse.
Flow Cytometry: CD8 Antibody (53-6.7) [NBP1-49045] - Analysis of fixed murine splenocytes by multiple staining.

Product Details

Reactivity Mu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, Func
1 mg/ml

Order Details

CD8 Antibody (53-6.7) Summary

This CD8 alpha antibody serves as an effective marker of cytotoxic T lymphocytes by binding to the CD8 co-receptor expressed on the cell surface of cytotoxic T cells, recognizing the topological domain of CD8 alpha. Because this CD8 antibody is made to the alpha chain it will recognize both the CD8 alpha - CD8 beta heterodimer, the most common form, as well as the CD8 alpha - CD8 alpha homodimer. The CD8A gene is also expressed in natural killer cells (NK cells), dendritic cells and cortical thymocytes making the CD8 alpha antibody a potential marker for these cells.
CD8 Antibody (53-6.7) was developed against mouse thymus or spleen.
Most thymocytes, T cell subset, some NK cells
IgG2a Kappa
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10 - 1:500
  • Cell depletion
  • CyTOF-ready
  • Inhibition of T Cell Function
Application Notes
Each lot of this CD8a antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is <0.25 ug/10^6 cells in 100 uL volume. It is recommended that the reagent be titrated for optimal performance for each application. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of both acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Use in ICC/IF was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24565643).
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 2 Reviews rated 5
NBP1-49045 in the following applications:

Read Publications using
NBP1-49045 in the following applications:

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1 mg/ml
Protein A or G purified

Alternate Names for CD8 Antibody (53-6.7)

  • 53-6.7 CD8 Alpha
  • 53-6.7 CD8 Aplha
  • 53-6.7 CD8
  • 53-6.7 Clone
  • 53-6.7
  • CD_antigen: CD8a
  • CD8 Antibody
  • CD8 antigen, alpha polypeptide (p32)
  • CD8
  • CD8a molecule
  • CD8A
  • Cytotoxic T cell marker
  • Cytotoxic T lymphocyte marker
  • Leu2 T-lymphocyte antigen
  • LEU2
  • MAL
  • OKT8 T-cell antigen
  • p32
  • T cell co-receptor
  • T cell marker
  • T8 T-cell antigen
  • T-cell antigen Leu2
  • T-cell surface glycoprotein CD8 alpha chain
  • T-lymphocyte differentiation antigen T8/Leu-2


CD8, also known as Leu-2 or T8 in human and Lyt2 or Lyt3 in mouse, is a cell surface glycoprotein belonging to the immunoglobulin supergene family (1, 2). CD8 is expressed on cytotoxic T-lymphocytes (T-cells), most thymocytes, between 35-45% of peripheral blood lymphocytes, and a population of natural killer (NK) cells (1, 2). The CD8 molecule consists of disulfide-linked alpha (alpha) and beta (beta) chains that present on T-cells as either CD8alphaalpha homodimers or CD8alphabeta heterodimers (1, 3). Both alpha and beta chains consist of a signaling sequence, an extracellular Ig-like domain, a membrane proximal stalk region, a transmembrane domain, and a cytoplasmic tail (3). Human CD8alpha is processed as 235 amino acids (aa) in length with a theoretical molecular weight of ~26 kDa, while mouse CD8alpha is 247 aa and has a theoretical molecular weight of 27.5 kDa (4, 5). Functionally, CD8 acts as an antigen coreceptor on cytotoxic T-cells and interacts with the major histocompatibility complex (MHC) class I molecules on antigen presenting cells (APCs), mediating cell-cell interactions within the immune system. Conversely, CD4 molecules interact with antigens presented on MHC class II molecules and are activated to become helper T-cells (TH) (1,2). Interestingly, thymocytes can transiently express both CD4 and CD8 during the maturation process (2). Furthermore, the cytoplasmic tail of CD8 has a Lck (lymphocyte-specific protein tyrosine kinase) binding domain where Lck interacts with CD8, initiating a phosphorylation cascade that activates transcription factors and promotes T-cell activation (6). More specifically, CD8alphabeta functions as a T-cell co-receptor, while CD8alphaalpha promotes T-cell survival and differentiation (7).

Given its role in the immune system, CD8-deficiency in T-cells is a hallmark of many diseases and pathologies (8-10). Specifically, CD8+ T-cell deficiency is prevalent in chronic autoimmune diseases including multiple sclerosis, rheumatoid arthritis, ulcerative colitis, Crohn's disease, type 1 diabetes mellitus, and Graves' disease (8). Furthermore, cancers or chronic infection can lead to CD8 T-cell exhaustion as the continual antigen presentation and inflammatory signals eventually cause the CD8+ T-cells to lose functionality (9, 10). However, animal models and clinical studies have suggested that T-cells are capable of being reinvigorated using inhibitory receptor blockade resulting in better disease outcomes and these exhausted T-cells may be a potential therapeutic target (9, 10).

Alternative names for CD8 includes CD antigen: CD8a, CD8 antigen, alpha polypeptide (p32), CD8a molecule, CD8A, Leu2 T-lymphocyte antigen, LEU2, MAL, OKT8 T-cell antigen, p32, T cell co-receptor, T8 T-cell antigen, T-cell antigen Leu2, T-cell surface glycoprotein CD8 alpha chain, and T-lymphocyte differentiation antigen T8/Leu-2.


1. Littman D. R. (1987). The structure of the CD4 and CD8 genes. Annual review of immunology.

2. Naeim F. (2008). Chapter 2- Principles of Immunophenotyping. Hematopathology.

3. Gao, G. F., & Jakobsen, B. K. (2000). Molecular interactions of coreceptor CD8 and MHC class I: the molecular basis for functional coordination with the T-cell receptor. Immunology today.

4. UniProt (P01732)

5. UniProt (P01731)

6. Kappes D. J. (2007). CD4 and CD8: hogging all the Lck. Immunity.

7. Gangadharan, D., & Cheroutre, H. (2004). The CD8 isoform CD8alphaalpha is not a functional homologue of the TCR co-receptor CD8alphabeta. Current opinion in immunology.

8. Pender M. P. (2012). CD8+ T-Cell Deficiency, Epstein-Barr Virus Infection, Vitamin D Deficiency, and Steps to Autoimmunity: A Unifying Hypothesis. Autoimmune diseases.

9. Kurachi M. (2019). CD8+ T cell exhaustion. Seminars in immunopathology.

10. Hashimoto, M., Kamphorst, A. O., Im, S. J., Kissick, H. T., Pillai, R. N., Ramalingam, S. S., Araki, K., & Ahmed, R. (2018). CD8 T Cell Exhaustion in Chronic Infection and Cancer: Opportunities for Interventions. Annual review of medicine.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD8 Antibody (NBP1-49045)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: FLOW, Flow, ICC/IF, IHC, IHC-P.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP1-49045 Applications Species
Block MS, Dietz AB, Gustafson MP et al. Th17-inducing autologous dendritic cell vaccination promotes antigen-specific cellular and humoral immunity in ovarian cancer patients Nat Commun Oct 14 2020 [PMID: 33057068] (IHC, Human) IHC Human
Hoover AA, Hufnagel DH, Harris W et al. Increased canonical NF-kappaB signaling specifically in macrophages is sufficient to limit tumor progression in syngeneic murine models of ovarian cancer BMC Cancer Oct 7 2020 [PMID: 33028251] (IHC-P, Mouse) IHC-P Mouse
Samanta D, Huang T, Shah R et al. BIRC2 Expression Impairs Anti-Cancer Immunity and Immunotherapy Efficacy Cell Rep [PMID: 32846130] (Flow, Mouse)

Citation using the PE format of this antibody.
Flow Mouse
Westrich JA, Vermeer DW, Silva A et al. CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8+ T-cell responses by upregulating MHC-I expression Oncogene Aug 15 2019 [PMID: 31417179] (ICC/IF, Mouse) ICC/IF Mouse
Fazia T, Pastorino R, Notartomaso S et al. Acid Sensing Ion Channel 2: a new potential player in the pathophysiology of multiple sclerosis Eur. J. Neurosci. Dec 14 2018 [PMID: 30549327] (IHC, Mouse) IHC Mouse
Samanta D, Park Y, Ni X et al. Chemotherapy induces enrichment of CD47+/CD73+/PDL1+ immune evasive triple-negative breast cancer cells Proc. Natl. Acad. Sci. U.S.A. 2018 Jan 24 [PMID: 29367423] (Flow, Mouse)

This citation used the PE form of this antbody
Flow Mouse
Sierra RA, Trillo-Tinoco J, Mohamed E et al. Anti-Jagged immunotherapy inhibits MDSCs and overcomes tumor-induced tolerance Cancer Res. 2017 Sep 13 [PMID: 28904063] (ICC/IF, Mouse) ICC/IF Mouse
Ma S, Deng W, Liu J et al. Blockade of adenosine A2A receptor enhances CD8+ T cells response and decreases regulatory T cells in head and neck squamous cell carcinoma. Mol Cancer 2017 Jun 07 [PMID: 28592285] (IHC, Mouse) IHC Mouse
Cohen N, Shani O, Raz Y et al. Fibroblasts drive an immunosuppressive and growth-promoting microenvironment in breast cancer via secretion of Chitinase 3-like 1. Oncogene. Apr 3 2017 [PMID: 28368410] (ICC/IF, Mouse) ICC/IF Mouse
Li A, Wu Y, Tang F et al. In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice. Cancer Biother. Radiopharm. Nov 1 2016 [PMID: 27831762] (Mouse) Mouse
Show All 16 Publications.

Reviews for CD8 Antibody (NBP1-49045) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using NBP1-49045:
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin CD8 NBP1-49045
reviewed by:
IHC-P Mouse 10/23/2017


Sample Testedmouse Head and neck squamous cell carcinoma (HNSCC)


Commentssections were incubated with antibody for CD8 alpha (Novus, 1:200, for mouse HNSCC samples) at 4 °C for 12 h.
Immunohistochemistry-Paraffin CD8 NBP1-49045
reviewed by:
Naiche Adler
IHC-P Mouse 12/15/2014


Sample TestedMouse Spleen

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for CD8 Antibody (NBP1-49045). (Showing 1 - 1 of 1 FAQs).

  1. Could you provide me with any suggestions for CD8 antibodies that work well with a CD4 antibody?
    • NBP1-28254, NBP1-28336, NBP1-28238, NBP1-49045 and NBP2-80658 have images of mostly Flow Cytometry analyses for CD4 and CD8. NB200-578 induces transplantation tolerance when used in conjunction with CD4 antibodies. I would suggest taking a look at these products to determine if they would be suitable for your experiments.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-49045AF350
Alexa Fluor 405 NBP1-49045AF405
Alexa Fluor 488 NBP1-49045AF488
Alexa Fluor 532 NBP1-49045AF532
Alexa Fluor 594 NBP1-49045AF594
Alexa Fluor 647 NBP1-49045AF647
Alexa Fluor 700 NBP1-49045AF700
Alexa Fluor 750 NBP1-49045AF750
Allophycocyanin NBP1-49045APC
Allophycocyanin/Cy7 NBP1-49045APCCY7
Biotin NBP1-49045B
DyLight 350 NBP1-49045UV
DyLight 405 NBP1-49045V
DyLight 488 NBP1-49045G
DyLight 550 NBP1-49045R
DyLight 650 NBP1-49045C
DyLight 680 NBP1-49045FR
DyLight 755 NBP1-49045IR
Janelia Fluor 549 NBP1-49045JF549
Janelia Fluor 646 NBP1-49045JF646
PE NBP1-49045PE
PE/Atto594 NBP1-49045PEATT594
PE/Cy5.5 NBP1-49045PECY55
PE/Cy7 NBP1-49045PECY7
PerCP NBP1-49045PCP

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Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

The CD4 Antibody: More than Just a Cellular Marker
CD4 is a member of the cluster of differentiation family of proteins, mainly expressed on the surface of thymocytes and a specific subset of mature T-cells. CD4 antibody studies have also shown it expressed on monocytes, cortical cells, microglial cel...  Read full blog post.

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Recent Reviews


Application: IHC-P
Species: Mouse

Naiche Adler
Application: IHC-P
Species: Mouse


Gene Symbol CD8A