FoxP3 Antibody [Biotin] Summary
A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Protein G purified
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- Western Blot
This FOXP3 antibody can be used for Immunoprecipitation, Immunohistochemistry paraffin embeded sections and Western blot analysis, where a band is seen ~48 kDa in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates. In IP transfected FOXP3-HA tagged proteins were used. In IHC-P, staining was observed in the nuclei of mouse spleen cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
This antibody shows reactivity against mouse CD4+CD25+T cells. There appears to be no reactivity against human Jurkat and mouse CD4+CD25-T cell lysates. Human reactivity reported in scientific literature (PMID: 23922187). Rat reactivity reported in scientific literature (PMID: 27758771)
Packaging, Storage & Formulations
Store at 4C in the dark.
0.05% Sodium Azide
Protein G purified
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for FoxP3 Antibody [Biotin]
- forkhead box P3
- Forkhead Box Protein P3
- immune dysregulation, polyendocrinopathy, enteropathy, X-linked
- Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked
- XPIDpolyendocrinopathy, enteropathy, X-linked
FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
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FAQs for FoxP3 Antibody (NB100-39002B). (Showing 1 - 3 of 3 FAQ).
Does FoxP3 antibodies comes in lyophilized form?
- FoxP3 antibodies, aka JM2 IPEX, and Scurfin antibodies, have the following products in lyophilized form: NBP1-18319, AF3240.
What the theoretical molecular weight for FoxP3 antibodies?
- The TMW of FoxP3 antibodies is approximately 47 - 52 kDa.
What is the immunogen sequence of this FoxP3 antibody?
- A sequence corresponding to amino acids 43 - 100 of Mouse FoxP3.
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Bioinformatics Tool for FoxP3 Antibody (NB100-39002B)
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Blogs on FoxP3.
Is has been established that the regulatory transcription factor FOXP3 (a member of the forkhead/winged-helix family of transcription factors) is imperative to immune system homeostasis through CD4+CD25+ regulatory T cell function. Distinctively,... Read full blog post.
FOXP3 is a Master Regulator of T Regulatory (Treg) Cells
FOXP3, a member of forkhead/winged-helix family of transcription factors acts as a "master" regulator for the development and suppressive function of regulatory T cells (Tregs). Its constitutive expression is necessary for the suppressive function of... Read full blog post.
FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom... Read full blog post.