Recombinant Human IL-2 Protein

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1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
Source
E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
202-IL in the following application:

Publications
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202-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

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Publications for IL-2 (202-IL)(269)

We have publications tested in 9 confirmed species: Human, Mouse, Bat - Pteropus alecto, Bovine, Canine, Chinchilla, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 8 applications: Bioassay, CAR-T, CAR-T (Bioassay), CAR-T (Differentiation), ELISA (Standard), In Vivo, Luminex Development, Surface Plasmon Resonance.


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(2)
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(1)
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N/A
(4)
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Showing Publications 1 - 10 of 269. Show All 269 Publications.
Publications using 202-IL Applications Species
Y Kong, B Jia, C Zhao, DF Claxton, A Sharma, C Annageldiy, JS Fotos, H Zeng, RF Paulson, KS Prabhu, H Zheng Downregulation of CD73 associates with T cell exhaustion in AML patients J Hematol Oncol, 2019;12(1):40. 2019 [PMID: 31014364] (Bioassay, Human) Bioassay Human
W Yang, KW Lee, RM Srivastava, F Kuo, C Krishna, D Chowell, V Makarov, D Hoen, MG Dalin, L Wexler, R Ghossein, N Katabi, Z Nadeem, MA Cohen, SK Tian, N Robine, K Arora, H Geiger, P Agius, N Bouvier, K Huberman, K Vanness, JJ Havel, JS Sims, RM Samstein, R Mandal, J Tepe, I Ganly, AL Ho, N Riaz, RJ Wong, N Shukla, TA Chan, LGT Morris Immunogenic neoantigens derived from gene fusions stimulate T cell responses Nat. Med., 2019;0(0):. 2019 [PMID: 31011208] (Bioassay, Human) Bioassay Human
I Lorthois, M Simard, S Morin, R Pouliot Infiltration of T Cells into a Three-Dimensional Psoriatic Skin Model Mimics Pathological Key Features Int J Mol Sci, 2019;20(7):. 2019 [PMID: 30987186] (Bioassay, Human) Bioassay Human
S Fourati, SP Ribeiro, F Blasco Tav, A Talla, F Lefebvre, M Cameron, J Kaewkungwa, P Pitisuttit, S Nitayaphan, S Rerks-Ngar, JH Kim, R Thomas, PB Gilbert, GD Tomaras, RA Koup, NL Michael, MJ McElrath, R Gottardo, RP Sékaly Integrated systems approach defines the antiviral pathways conferring protection by the RV144 HIV vaccine Nat Commun, 2019;10(1):863. 2019 [PMID: 30787294] (Bioassay, Human) Bioassay Human
Y Luo, Y Xue, J Wang, J Dang, Q Fang, G Huang, N Olsen, SG Zheng Negligible Effect of Sodium Chloride on the Development and Function of TGF-?-Induced CD4+ Foxp3+ Regulatory T Cells Cell Rep, 2019;26(7):1869-1879.e3. 2019 [PMID: 30759396] (Bioassay, Human) Bioassay Human
L Herviou, M Jourdan, AM Martinez, G Cavalli, J Moreaux EZH2 is overexpressed in transitional preplasmablasts and is involved in human plasma cell differentiation Leukemia, 2019;0(0):. 2019 [PMID: 30755708] (Bioassay, Human) Bioassay Human
MH Abumaree, NA Alshehri, A Almotery, AM Al Subayyi, E Bahattab, FM Abomaray, T Khatlani, B Kalionis, D Jawdat, MF El-Muzaini, MA Al Jumah, AS AlAskar Preconditioning human natural killer cells with chorionic villous mesenchymal stem cells stimulates their expression of inflammatory and anti-tumor molecules Stem Cell Res Ther, 2019;10(1):50. 2019 [PMID: 30728068] (Bioassay, Human) Bioassay Human
N Zhang, Y Dou, L Liu, X Zhang, X Liu, Q Zeng, Y Liu, M Yin, X Liu, H Deng, D Song SA-49, a novel aloperine derivative, induces MITF-dependent lysosomal degradation of PD-L1 EBioMedicine, 2019;0(0):. 2019 [PMID: 30711516] (Bioassay, Human) Bioassay Human
C Schleiss, W Ilias, O Tahar, Y Güler, L Miguet, C Mayeur-Rou, L Mauvieux, LM Fornecker, E Toussaint, R Herbrecht, F Bertrand, M Maumy-Bert, T Martin, S Fournel, P Georgel, S Bahram, L Vallat BCR-associated factors driving chronic lymphocytic leukemia cells proliferation ex vivo Sci Rep, 2019;9(1):701. 2019 [PMID: 30679590] (Bioassay, Human) Bioassay Human
S Whelan, E Ophir, MF Kotturi, O Levy, S Ganguly, L Leung, I Vaknin, S Kumar, L Dassa, K Hansen, D Bernados, B Murter, A Soni, JM Taube, AN Fader, TL Wang, IM Shih, M White, DM Pardoll, SC Liang PVRIG and PVRL2 Are Induced in Cancer and Inhibit CD8+ T-cell Function Cancer Immunol Res, 2019;0(0):. 2019 [PMID: 30659054] (Bioassay, Human) Bioassay Human
Show All 269 Publications.

Reviews for IL-2 (202-IL) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 202-IL:
Filter by Applications
CellProlif
(2)
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reviewed by:
Anonymous
CellProlif 05/18/2019
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Summary

ApplicationCell Proliferation
LotAE6017121
 
reviewed by:
Anonymous
CellProlif 01/23/2019
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Summary

ApplicationCell Proliferation
Lot202

FAQs for IL-2 (202-IL) (0)

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Anonymous
05/18/2019
Application: CellProlif
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Anonymous
01/23/2019
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Bioinformatics

Gene Symbol IL2
Uniprot