Recombinant Human IL-2 Protein


1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.

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Recombinant Human IL-2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
13 kDa, reducing conditions
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202-IL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF


Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

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Publications for IL-2 (202-IL)(269)

We have publications tested in 9 confirmed species: Human, Mouse, Bat - Pteropus alecto, Bovine, Canine, Chinchilla, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 8 applications: Bioassay, CAR-T, CAR-T (Bioassay), CAR-T (Differentiation), ELISA (Standard), In Vivo, Luminex Development, Surface Plasmon Resonance.

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Bat - Pteropus alecto
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 269. Show All 269 Publications.
Publications using 202-IL Applications Species
Y Kong, B Jia, C Zhao, DF Claxton, A Sharma, C Annageldiy, JS Fotos, H Zeng, RF Paulson, KS Prabhu, H Zheng Downregulation of CD73 associates with T cell exhaustion in AML patients J Hematol Oncol, 2019;12(1):40. 2019 [PMID: 31014364] (Bioassay, Human) Bioassay Human
W Yang, KW Lee, RM Srivastava, F Kuo, C Krishna, D Chowell, V Makarov, D Hoen, MG Dalin, L Wexler, R Ghossein, N Katabi, Z Nadeem, MA Cohen, SK Tian, N Robine, K Arora, H Geiger, P Agius, N Bouvier, K Huberman, K Vanness, JJ Havel, JS Sims, RM Samstein, R Mandal, J Tepe, I Ganly, AL Ho, N Riaz, RJ Wong, N Shukla, TA Chan, LGT Morris Immunogenic neoantigens derived from gene fusions stimulate T cell responses Nat. Med., 2019;0(0):. 2019 [PMID: 31011208] (Bioassay, Human) Bioassay Human
I Lorthois, M Simard, S Morin, R Pouliot Infiltration of T Cells into a Three-Dimensional Psoriatic Skin Model Mimics Pathological Key Features Int J Mol Sci, 2019;20(7):. 2019 [PMID: 30987186] (Bioassay, Human) Bioassay Human
S Fourati, SP Ribeiro, F Blasco Tav, A Talla, F Lefebvre, M Cameron, J Kaewkungwa, P Pitisuttit, S Nitayaphan, S Rerks-Ngar, JH Kim, R Thomas, PB Gilbert, GD Tomaras, RA Koup, NL Michael, MJ McElrath, R Gottardo, RP Sékaly Integrated systems approach defines the antiviral pathways conferring protection by the RV144 HIV vaccine Nat Commun, 2019;10(1):863. 2019 [PMID: 30787294] (Bioassay, Human) Bioassay Human
Y Luo, Y Xue, J Wang, J Dang, Q Fang, G Huang, N Olsen, SG Zheng Negligible Effect of Sodium Chloride on the Development and Function of TGF-?-Induced CD4+ Foxp3+ Regulatory T Cells Cell Rep, 2019;26(7):1869-1879.e3. 2019 [PMID: 30759396] (Bioassay, Human) Bioassay Human
L Herviou, M Jourdan, AM Martinez, G Cavalli, J Moreaux EZH2 is overexpressed in transitional preplasmablasts and is involved in human plasma cell differentiation Leukemia, 2019;0(0):. 2019 [PMID: 30755708] (Bioassay, Human) Bioassay Human
MH Abumaree, NA Alshehri, A Almotery, AM Al Subayyi, E Bahattab, FM Abomaray, T Khatlani, B Kalionis, D Jawdat, MF El-Muzaini, MA Al Jumah, AS AlAskar Preconditioning human natural killer cells with chorionic villous mesenchymal stem cells stimulates their expression of inflammatory and anti-tumor molecules Stem Cell Res Ther, 2019;10(1):50. 2019 [PMID: 30728068] (Bioassay, Human) Bioassay Human
N Zhang, Y Dou, L Liu, X Zhang, X Liu, Q Zeng, Y Liu, M Yin, X Liu, H Deng, D Song SA-49, a novel aloperine derivative, induces MITF-dependent lysosomal degradation of PD-L1 EBioMedicine, 2019;0(0):. 2019 [PMID: 30711516] (Bioassay, Human) Bioassay Human
C Schleiss, W Ilias, O Tahar, Y Güler, L Miguet, C Mayeur-Rou, L Mauvieux, LM Fornecker, E Toussaint, R Herbrecht, F Bertrand, M Maumy-Bert, T Martin, S Fournel, P Georgel, S Bahram, L Vallat BCR-associated factors driving chronic lymphocytic leukemia cells proliferation ex vivo Sci Rep, 2019;9(1):701. 2019 [PMID: 30679590] (Bioassay, Human) Bioassay Human
S Whelan, E Ophir, MF Kotturi, O Levy, S Ganguly, L Leung, I Vaknin, S Kumar, L Dassa, K Hansen, D Bernados, B Murter, A Soni, JM Taube, AN Fader, TL Wang, IM Shih, M White, DM Pardoll, SC Liang PVRIG and PVRL2 Are Induced in Cancer and Inhibit CD8+ T-cell Function Cancer Immunol Res, 2019;0(0):. 2019 [PMID: 30659054] (Bioassay, Human) Bioassay Human
Show All 269 Publications.

Reviews for IL-2 (202-IL) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 202-IL:
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reviewed by:
CellProlif 05/18/2019


ApplicationCell Proliferation
reviewed by:
CellProlif 01/23/2019


ApplicationCell Proliferation

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Gene Symbol IL2