Recombinant Human IL-2 Protein

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1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Catalog# & Formulation Size Price

Recombinant Human IL-2 Protein Summary

Additional Information
Version with mutation found in aldesleukin Available!
Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
Source
E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
202-IL in the following application:

Publications
Read Publications using
202-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

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Publications for IL-2 (202-IL)(300)

We have publications tested in 9 confirmed species: Human, Mouse, Bat - Pteropus alecto, Bovine, Canine, Chinchilla, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 9 applications: Bioassay, CAR-T, CAR-T (Bioassay), CAR-T (Differentiation), Cell Culture, ELISA (Standard), In Vivo, Luminex Development, Surface Plasmon Resonance.


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(275)
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(4)
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(2)
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(1)
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(1)
N/A
(4)
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Showing Publications 1 - 10 of 300. Show All 300 Publications.
Publications using 202-IL Applications Species
A Mauriello, C Manolio, B Cavalluzzo, A Avallone, M Borrelli, A Morabito, E Iovine, A Chambery, R Russo, ML Tornesello, FM Buonaguro, M Tagliamont, L Buonaguro Immunological effects of adjuvants in subsets of antigen presenting cells of cancer patients undergoing chemotherapy J Transl Med, 2020;18(1):34. 2020 [PMID: 31973714] (Cell Culture, Human) Cell Culture Human
JB McBrien, M Mavigner, L Franchitti, SA Smith, E White, GK Tharp, H Walum, K Busman-Suh, CR Aguilera-S, WO Thayer, RA Spagnuolo, M Kovarova, A Wahl, B Cervasi, DM Margolis, TH Vanderford, DG Carnathan, M Paiardini, JD Lifson, JH Lee, JT Safrit, SE Bosinger, JD Estes, CA Derdeyn, JV Garcia, DA Kulpa, A Chahroudi, G Silvestri Robust and persistent reactivation of SIV and HIV by N-803 and depletion of CD8+ cells Nature, 2020;0(0):. 2020 [PMID: 31969705] (Cell Culture, Mouse) Cell Culture Mouse
J Brzostek, N Gautam, X Zhao, EW Chen, M Mehta, DWH Tung, YL Chua, J Yap, SH Cho, S Sankaran, V Rybakin, G Fu, NRJ Gascoigne T cell receptor and cytokine signal integration in CD8+ T cells is mediated by the protein Themis Nat. Immunol., 2020;0(0):. 2020 [PMID: 31932808] (Cell Culture, Mouse) Cell Culture Mouse
D Gate, N Saligrama, O Leventhal, AC Yang, MS Unger, J Middeldorp, K Chen, B Lehallier, D Channappa, MB De Los San, A McBride, J Pluvinage, F Elahi, GK Tam, Y Kim, M Greicius, AD Wagner, L Aigner, DR Galasko, MM Davis, T Wyss-Coray Clonally expanded CD8 T cells patrol the cerebrospinal fluid in Alzheimer&#039;s disease Nature, 2020;577(7790):399-404. 2020 [PMID: 31915375] (Cell Culture, Human) Cell Culture Human
JJ Montoya, MA Turnidge, DH Wai, AR Patel, DW Lee, V Gokhale, LH Hurley, RJ Arceci, C Wetmore, DO Azorsa In vitro activity of a G-quadruplex-stabilizing small molecule that synergizes with Navitoclax to induce cytotoxicity in acute myeloid leukemia cells BMC Cancer, 2019;19(1):1251. 2019 [PMID: 31881855] (Bioassay, Human) Bioassay Human
N An, H Wang, W Jia, W Jing, C Liu, H Zhu, J Yu The prognostic role of circulating CD8+ T cell proliferation in patients with untreated extensive stage small cell lung cancer J Transl Med, 2019;17(1):402. 2019 [PMID: 31796037] (Bioassay, Human) Bioassay Human
A Koenig, CC Chen, A Marçais, T Barba, V Mathias, A Sicard, M Rabeyrin, M Racapé, JP Duong-Van-, P Bruneval, A Loupy, S Dussurgey, S Ducreux, V Meas-Yedid, JC Olivo-Mari, H Paidassi, R Guillemain, JL Taupin, J Callemeyn, E Morelon, A Nicoletti, B Charreau, V Dubois, M Naesens, T Walzer, T Defrance, O Thaunat Missing self triggers NK cell-mediated chronic vascular rejection of solid organ transplants Nat Commun, 2019;10(1):5350. 2019 [PMID: 31767837] (Cell Culture, Human) Cell Culture Human
M Yamagishi, M Hori, D Fujikawa, T Ohsugi, D Honma, N Adachi, H Katano, T Hishima, S Kobayashi, K Nakano, M Nakashima, M Iwanaga, A Utsunomiya, Y Tanaka, S Okada, K Tsukasaki, K Tobinai, K Araki, T Watanabe, K Uchimaru Targeting Excessive EZH1 and EZH2 Activities for Abnormal Histone Methylation and Transcription Network in Malignant Lymphomas Cell Rep, 2019;29(8):2321-2337.e7. 2019 [PMID: 31747604] (Cell Culture, Human) Cell Culture Human
X Huan, Z Zhuo, Z Xiao, EC Ren Crystal structure of suboptimal viral fragments of Epstein Barr Virus Rta peptide-HLA complex that stimulate CD8 T cell response Sci Rep, 2019;9(1):16660. 2019 [PMID: 31723204] (Cell Culture, Human) Cell Culture Human
F Caccuri, A Bugatti, S Corbellini, S Roversi, A Zani, P Mazzuca, S Marsico, A Caruso, C Giagulli The Synthetic Dipeptide Pidotimod Shows a Chemokine-Like Activity through CXC Chemokine Receptor 3 (CXCR3) Int J Mol Sci, 2019;20(21):. 2019 [PMID: 31653015] (Bioassay, Human) Bioassay Human
Show All 300 Publications.

Reviews for IL-2 (202-IL) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 202-IL:
Filter by Applications
CellProlif
(2)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
CellProlif 05/18/2019
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Summary

ApplicationCell Proliferation
LotAE6017121
 
reviewed by:
Anonymous
CellProlif 01/23/2019
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Summary

ApplicationCell Proliferation
Lot202

FAQs for IL-2 (202-IL). (Showing 1 - 1 of 1 FAQs).

  1. we bought the rHIL-2 protein ref 202-IL-500 and it says that the reconstitution has to be done in acetic acid, but I need to use the protein in PBS, is that possible to reconstitute it directly in PBS instead? would it be stable?
    • It will not be soluble in PBS alone so 100 mM acetic acid is required for it to go into solution. Then you can dilute into PBS only and that should not affect your experimentation.

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Recent Reviews

5
5
2
4
0
3
0
2
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1
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Anonymous
05/18/2019
Application: CellProlif
Species:

 
Anonymous
01/23/2019
Application: CellProlif
Species:

Bioinformatics

Gene Symbol IL2
Uniprot