Recombinant Human IL-2 Protein

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Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED 50 for this effect is 0.05-0.25 ng/mL. The specific activity of Recombinant Human IL-2 is approximately 2.1 x 10 4 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
Source
E. coli-derived human IL-2 protein Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Publications
Read Publications using
202-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein

  • aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4 + and CD8 + T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4 + T cells but not activated CD4 + memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).
  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

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Publications for IL-2 (202-IL)(236)

We have publications tested in 8 confirmed species: Human, Mouse, Bat - Pteropus alecto, Bovine, Canine, Chinchilla, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 4 applications: Bioassay, ELISA Standard, In Vivo, Luminex Development.


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Bioassay
(228)
ELISA Standard
(4)
In Vivo
(2)
Luminex Development
(1)
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Human
(196)
Mouse
(29)
Bat - Pteropus alecto
(1)
Bovine
(1)
Canine
(1)
Chinchilla
(1)
N/A
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
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Showing Publications 1 - 10 of 236. Show All 236 Publications.
Publications using 202-IL Applications Species
CY Kuo, JD Long, B Campo-Fern, S de Oliveir, AR Cooper, Z Romero, MD Hoban, AV Joglekar, GR Lill, ML Kaufman, S Fitz-Gibbo, X Wang, RP Hollis, DB Kohn Site-Specific Gene Editing of Human Hematopoietic Stem Cells for X-Linked Hyper-IgM Syndrome Cell Rep, 2018;23(9):2606-2616. 2018 [PMID: 29847792] (Bioassay, Human) Bioassay Human
A Schmidt, F Marabita, NA Kiani, CC Gross, HJ Johansson, S Éliás, S Rautio, M Eriksson, SJ Fernandes, G Silberberg, U Ullah, U Bhatia, H Lähdesmäki, J Lehtiö, D Gomez-Cabr, H Wiendl, R Lahesmaa, J Tegnér Time-resolved transcriptome and proteome landscape of human regulatory T cell (Treg) differentiation reveals novel regulators of FOXP3 BMC Biol., 2018;16(1):47. 2018 [PMID: 29730990] (Bioassay, Human) Bioassay Human
Y He, Y Guo, J Chen, X Hu, X Li, Y Kong, X Zhang, X Zhou, L Liu, J Hou Multiple Antigen Stimulating Cellular Therapy (MASCT) For Hepatocellular Carcinoma After Curative Treatment: A Retrospective Study J Cancer, 2018;9(8):1385-1393. 2018 [PMID: 29721048] (Bioassay, Human) Bioassay Human
C Engdahl, A Bondt, U Harre, J Raufer, R Pfeifle, A Camponesch, M Wuhrer, M Seeling, IL Mårtensson, F Nimmerjahn, G Krönke, HU Scherer, H Forsblad-d, G Schett Estrogen induces St6gal1 expression and increases IgG sialylation in mice and patients with rheumatoid arthritis: a potential explanation for the increased risk of rheumatoid arthritis in postmenopausal women Arthritis Res. Ther., 2018;20(1):84. 2018 [PMID: 29720252] (Bioassay, Human) Bioassay Human
S Tanaka, C Pfleger, JF Lai, F Roan, SC Sun, SF Ziegler KAP1 Regulates Regulatory T Cell Function and Proliferation in Both Foxp3-Dependent and -Independent Manners Cell Rep, 2018;23(3):796-807. 2018 [PMID: 29669285] (Bioassay, Mouse) Bioassay Mouse
MH Abumaree, E Bahattab, A Alsadoun, A Al Dosaima, FM Abomaray, T Khatlani, B Kalionis, MF El-Muzaini, AO Alawad, AS AlAskar Characterization of the interaction between human decidua parietalis mesenchymal stem/stromal cells and natural killer cells Stem Cell Res Ther, 2018;9(1):102. 2018 [PMID: 29650045] (Bioassay, Human) Bioassay Human
H Pei, L He, M Shao, Z Yang, Y Ran, D Li, Y Zhou, M Tang, T Wang, Y Gong, X Chen, S Yang, M Xiang, L Chen Discovery of a highly selective JAK3 inhibitor for the treatment of rheumatoid arthritis Sci Rep, 2018;8(1):5273. 2018 [PMID: 29588471] (Bioassay, Human) Bioassay Human
S Nagel, C Pommerenke, C Meyer, M Kaufmann, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox gene HLX in Hodgkin lymphoma Oncotarget, 2018;9(18):14338-14353. 2018 [PMID: 29581848] (Bioassay, Human) Bioassay Human
X Chen, S Hao, Z Zhao, J Liu, Q Shao, F Wang, D Sun, Y He, W Gao, H Mao Interleukin 35: Inhibitory regulator in monocyte-derived dendritic cell maturation and activation Cytokine, 2018;108(0):43-52. 2018 [PMID: 29571039] (Bioassay, Human) Bioassay Human
PO Flores-Vil, M Ganachari, H Guio, JA Mejia, J Granados An Isolated TCR ?? Restricted by HLA-A*02:01/CT37 Peptide Redirecting CD8+T Cells To Kill and Secrete IFN-? in Response to Lung Adenocarcinoma Cell Lines J. Immunol., 2018;0(0):. 2018 [PMID: 29555781] (Bioassay, Human) Bioassay Human
Show All 236 Publications.

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Bioinformatics

Gene Symbol IL2
Entrez
Uniprot