Recombinant Human IL-2 Protein

Images

 
There are currently no images for IL-2 (202-IL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-2 Protein Summary

Additional Information
Version with mutation found in aldesleukin Available!
Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 2.1 x 104 IU/μg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).
Source
E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Reviewed Applications
Read 3 Reviews rated 5
using
202-IL in the following application:

Publications
Read Publications using
202-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid containing at least 0.1% human or bovine serum albumin.  Alternatively, reconstitute at 100 μg/mL in sterile deionized water and use within 24 hours, store at 2 to 8 °C.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

Customers Who Viewed This Item Also Viewed...

NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-49045
Species: Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Cell Depl, CyTOF-ready, InhibTFunc
NBP2-45041
Species: Hu, Po
Applications: Flow, ICC/IF, IF
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, Flow-IC
MAB342
Species: Hu
Applications: WB, AgAct
H00003669-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, S-ELISA
NBP1-88988
Species: Hu
Applications: WB, ICC/IF, KD
202-IL
Species: Hu
Applications: Bioactivity

Publications for IL-2 (202-IL)(314)

We have publications tested in 9 confirmed species: Human, Mouse, Bat - Pteropus alecto, Bovine, Canine, Chinchilla, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 9 applications: Bioassay, CAR-T, CAR-T (Bioassay), CAR-T (Differentiation), Cell Culture, ELISA (Standard), In Vivo, Luminex Development, Surface Plasmon Resonance.


Filter By Application
Bioassay
(278)
CAR-T
(1)
CAR-T (Bioassay)
(2)
CAR-T (Differentiation)
(1)
Cell Culture
(22)
ELISA (Standard)
(4)
In Vivo
(2)
Luminex Development
(1)
Surface Plasmon Resonance
(1)
All Applications
Filter By Species
Human
(266)
Mouse
(36)
Bat - Pteropus alecto
(1)
Bovine
(1)
Canine
(1)
Chinchilla
(1)
Feline
(1)
N/A
(4)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
All Species
Showing Publications 1 - 10 of 314. Show All 314 Publications.
Publications using 202-IL Applications Species
M Bachsais, S Salti, K Zaoui, GS Hassan, F Aoudjit, W Mourad CD154 inhibits death of T cells via a Cis interaction with the &amp;alpha5&amp;beta1 integrin PLoS ONE, 2020;15(8):e0235753. 2020 [PMID: 32745080] (Cell Culture, Human) Cell Culture Human
IJ Beccard, L Hofmann, JC Schroeder, S Ludwig, S Laban, C Brunner, R Lotfi, TK Hoffmann, EK Jackson, PJ Schuler, MN Theodoraki Immune Suppressive Effects of Plasma-Derived Exosome Populations in Head and Neck Cancer Cancers (Basel), 2020;12(7):. 2020 [PMID: 32708274] (Cell Culture, Human) Cell Culture Human
W Gao, S Xu, M Zhang, S Liu, SP Siu, H Peng, JC Ng, GS Tsao, AW Chan, VL Chow, JY Chan, TS Wong NADPH oxidase 5&amp;alpha promotes the formation of CD271 tumor-initiating cells in oral cancer Am J Cancer Res, 2020;10(6):1710-1727. 2020 [PMID: 32642285] (Cell Culture, Human) Cell Culture Human
Y Wan, C Zhang, Y Xu, M Wang, Q Rao, H Xing, Z Tian, K Tang, Y Mi, Y Wang, J Wang Hyperfunction of CD4 CD25 regulatory T cells in de novo acute myeloid leukemia BMC Cancer, 2020;20(1):472. 2020 [PMID: 32456622] (Cell Culture, Human) Cell Culture Human
K Froning, J Maguire, A Sereno, F Huang, S Chang, K Weichert, AJ Frommelt, J Dong, X Wu, H Austin, EM Conner, JR Fitchett, AR Heng, D Balasubram, MT Hilgers, B Kuhlman, SJ Demarest Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics Nat Commun, 2020;11(1):2330. 2020 [PMID: 32393818] (Bioassay, Human) Bioassay Human
Y Hu, H Liu, C Fang, C Li, F Xhyliu, H Dysert, J Bodo, G Habermehl, BE Russell, W Li, M Chappell, X Jiang, SL Ondrejka, ED Hsi, JP Maciejewsk, Q Yi, KC Anderson, NC Munshi, G Ao, JN Valent, J Lin, J Zhao Targeting of CD38 by the tumor suppressor miR-26a serves as a novel potential therapeutic agent in multiple myeloma Cancer Res., 2020;0(0):. 2020 [PMID: 32193289] (Cell Culture, Human) Cell Culture Human
T Chirkova, B Ha, BH Rimawi, AGP Oomens, TV Hartert, LJ Anderson In vitro model for the assessment of human immune responses to subunit RSV vaccines PLoS ONE, 2020;15(3):e0229660. 2020 [PMID: 32191728] (Cell Culture, Human) Cell Culture Human
AE Morelli, TL Sumpter, DM Rojas-Cana, M Bandyopadh, Z Chen, O Tkacheva, WJ Shufesky, CT Wallace, SC Watkins, A Berger, CJ Paige, LD Falo, AT Larregina Neurokinin-1 Receptor Signaling Is Required for Efficient Ca2+ Flux in T-Cell-Receptor-Activated T Cells Cell Rep, 2020;30(10):3448-3465.e8. 2020 [PMID: 32160549] (Bioassay, Mouse) Bioassay Mouse
S Zicari, AL Sharma, G Sahu, L Dubrovsky, L Sun, H Yue, T Jada, A Ochem, G Simon, M Bukrinsky, M Tyagi DNA dependent protein kinase (DNA-PK) enhances HIV transcription by promoting RNA polymerase II activity and recruitment of transcription machinery at HIV LTR Oncotarget, 2020;11(7):699-726. 2020 [PMID: 32133046] (Cell Culture, Human) Cell Culture Human
Y Gong, P Chowdhury, PKB Nagesh, MA Rahman, K Zhi, MM Yallapu, S Kumar Novel elvitegravir nanoformulation for drug delivery across the blood-brain barrier to achieve HIV-1 suppression in the CNS macrophages Sci Rep, 2020;10(1):3835. 2020 [PMID: 32123217] (Cell Culture, Human) Cell Culture Human
Show All 314 Publications.

Reviews for IL-2 (202-IL) (3) 53

Average Rating: 5
(Based on 3 reviews)

Reviews using 202-IL:
Filter by Applications
CellProlif
(3)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
CellProlif 03/31/2021
View

Summary

ApplicationCell Proliferation
LotAE6419102
Commentshigh concentration.
  5
reviewed by:
Anonymous
CellProlif 05/18/2019
View

Summary

ApplicationCell Proliferation
LotAE6017121
  5
reviewed by:
Anonymous
CellProlif 01/23/2019
View

Summary

ApplicationCell Proliferation
Lot202

FAQs for IL-2 (202-IL). (Showing 1 - 1 of 1 FAQs).

  1. we bought the rHIL-2 protein ref 202-IL-500 and it says that the reconstitution has to be done in acetic acid, but I need to use the protein in PBS, is that possible to reconstitute it directly in PBS instead? would it be stable?
    • It will not be soluble in PBS alone so 100 mM acetic acid is required for it to go into solution. Then you can dilute into PBS only and that should not affect your experimentation.

Other Available Formats

Additional IL-2 Products

Bioinformatics Tool for IL-2 (202-IL)

Discover related pathways, diseases and genes to IL-2 (202-IL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-2 (202-IL)

Discover more about diseases related to IL-2 (202-IL).
 

Pathways for IL-2 (202-IL)

View related products by pathway.

PTMs for IL-2 (202-IL)

Learn more about PTMs related to IL-2 (202-IL).

Blogs on IL-2.

The role of HIF-2 alpha in the progression and therapy of clear cell renal cell carcinoma
HIF-2 alpha, also known as hypoxia-inducible factor 2, endothelial PAS domain protein-1, and member of PAS superfamily 2 is part of the HIF family of proteins.  The HIF family is composed of HIF-1, HIF-2 and HIF-3, where HIF-2 is a dimeric protein...  Read full blog post.

iNOS: The Nitric Oxide Boss
Nitric oxide (NO) is an inorganic, gaseous, and reactive free radical that acts as a biologic mediator in processes such as neurotransmission, vasorelaxation, and cytotoxicity. In addition, it has antimicrobial and anti-tumoral activities. NO producti...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
3
4
0
3
0
2
0
1
0

 
Anonymous
03/31/2021
Application: CellProlif
Species:

 
Anonymous
05/18/2019
Application: CellProlif
Species:

 
Anonymous
01/23/2019
Application: CellProlif
Species:

Bioinformatics

Gene Symbol IL2
Uniprot