Recombinant Human IL-2 Protein

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1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-2 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.05-0.25 ng/mL.
The specific activity of Recombinant Human IL-2 is approximately 9.1 x 106 IU/mg, which is calibrated against recombinant human IL-2 WHO International Standard (NIBSC code: 86/500).  Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human IL-2 protein
Ala21-Thr153, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL2
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
13 kDa, reducing conditions
Reviewed Applications
Read 4 Reviews rated 5
using
202-IL in the following application:

Publications
Read Publications using
202-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid containing at least 0.1% human or bovine serum albumin.  Alternatively, reconstitute at 100 μg/mL in sterile deionized water and use within 24 hours, store at 2 to 8 °C.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-2 Protein

  • Aldesleukin
  • IL2
  • IL-2
  • IL-2lymphokine
  • interleukin 2
  • interleukin-2
  • involved in regulation of T-cell clonal expansion
  • T cell growth factor
  • T-cell growth factor
  • TCGF

Background

Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils (1-3). Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit cross-species activity (4). The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes (5-7). The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta , which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma , which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to proliferate and induces IL-2 and IL-2 R alpha synthesis (1, 2). It contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes (8). IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells (9-11). Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (12, 13).

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
  3. Taniguchi, T. et al. (1983) Nature 302:305.
  4. Mosmann, T.R. et al. (1987) J. Immunol. 138:1813.
  5. Liparoto, S.F. et al. (2002) Biochemistry 41:2543.
  6. Wang, X. et al. (2005) Science 310:1159.
  7. Bodnar, A. et al. (2008) Immunol. Lett. 116:117.
  8. Jaleco, S. et al. (2003) J. Immunol. 171:61.
  9. Malek, T.R. (2003) J. Leukoc. Biol. 74:961.
  10. Laurence, A. et al. (2007) Immunity 26:371.
  11. Kryczek, I. et al. (2007) J. Immunol. 178:6730.
  12. Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32.
  13. Fehervari, Z. et al. (2006) Trends Immunol. 27:109.

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Publications for IL-2 (202-IL)(333)

We have publications tested in 9 confirmed species: Human, Mouse, Bat - Pteropus alecto, Bovine, Canine, Chinchilla, Feline, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 9 applications: Bioassay, CAR-T, CAR-T (Bioassay), CAR-T (Differentiation), Cell Culture, ELISA (Standard), In Vivo, Luminex Development, Surface Plasmon Resonance.


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Bioassay
(289)
CAR-T
(1)
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(2)
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(1)
Cell Culture
(30)
ELISA (Standard)
(4)
In Vivo
(2)
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(1)
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(1)
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(1)
N/A
(4)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 333. Show All 333 Publications.
Publications using 202-IL Applications Species
CH Kang, Y Kim, DY Lee, SU Choi, HK Lee, CH Park c-Met-Specific Chimeric Antigen Receptor T Cells Demonstrate Anti-Tumor Effect in c-Met Positive Gastric Cancer Cancers, 2021;13(22):. 2021 [PMID: 34830894] (Bioassay, Human) Bioassay Human
SA Saenz, A Local, T Carr, A Shakya, S Koul, H Hu, L Chourb, J Stedman, J Malley, LA D'Agostino, V Shanmugasu, J Malona, CE Schwartz, L Beebe, M Clements, G Rajaraman, J Cho, L Jiang, A Dubrovskiy, M Kreilein, R Shimanovic, LG Hamann, L Escoubet, JM Ellis Small molecule allosteric inhibitors of RORgammat block Th17-dependent inflammation and associated gene expression in vivo PLoS ONE, 2021;16(11):e0248034. 2021 [PMID: 34752458] (Bioassay, Human) Bioassay Human
P Prakhar, J Alvarez-De, H Keller, A Crossman, X Tai, YK Park, JH Park The small intestine epithelium exempts Foxp3+ Tregs from their IL-2 requirement for homeostasis and effector function JCI Insight, 2021;6(21):. 2021 [PMID: 34747370] (Bioassay, Mouse) Bioassay Mouse
MN Theodoraki, S Laban, EK Jackson, R Lotfi, PJ Schuler, C Brunner, TK Hoffmann, TL Whiteside, L Hofmann Changes in circulating exosome molecular profiles following surgery/(chemo)radiotherapy: early detection of response in head and neck cancer patients British Journal of Cancer, 2021;0(0):. 2021 [PMID: 34642463] (Bioassay, Human) Bioassay Human
S Yu, C Di, S Chen, M Guo, J Yan, Z Zhu, L Liu, R Feng, Y Xie, R Zhang, J Chen, M Wang, D Wei, H Fang, T Yin, J Huang, S Chen, H Lu, J Zhu, J Qu Distinct immune signatures discriminate between asymptomatic and presymptomatic SARS-CoV-2pos subjects Cell Research, 2021;0(0):. 2021 [PMID: 34561618] (Bioassay, Human) Bioassay Human
L Dai, Q Wang, X Lv, F Gao, Z Chen, Y Shen Elevated beta-secretase 1 expression mediates CD4+ T cell dysfunction via PGE2 signalling in Alzheimer&#039;s disease Brain, Behavior, and Immunity, 2021;98(0):337-348. 2021 [PMID: 34500034] (Bioassay, Mouse) Bioassay Mouse
KO Jang, YW Lee, H Kim, DK Chung Complement Inactivation Strategy of Staphylococcus aureus Using Decay-Accelerating Factor and the Response of Infected HaCaT Cells International Journal of Molecular Sciences, 2021;22(8):. 2021 [PMID: 33924622] (Cell Culture, Human) Cell Culture Human
AM Farhat, AC Weiner, C Posner, ZS Kim, B Orcutt-Jah, SM Carlson, AS Meyer Modeling cell-specific dynamics and regulation of the common gamma chain cytokines Cell Reports, 2021;35(4):109044. 2021 [PMID: 33910015] (Bioassay, Human) Bioassay Human
AT Tan, M Linster, CW Tan, N Le Bert, WN Chia, K Kunasegara, Y Zhuang, CYL Tham, A Chia, GJD Smith, B Young, S Kalimuddin, JGH Low, D Lye, LF Wang, A Bertoletti Early induction of functional SARS-CoV-2-specific T cells associates with rapid viral clearance and mild disease in COVID-19 patients Cell Reports, 2021;34(6):108728. 2021 [PMID: 33516277] (Bioassay, Human) Bioassay Human
JH Ruth, M Gurrea-Rub, KS Athukorala, SM Rasmussen, D Weber, PM Randon, RJ Gedert, ME Lind, MA Amin, PL Campbell, PS Tsou, Y Mao-Draaye, Q Wu, TM Lanigan, VG Keshamouni, NG Singer, F Lin, DA Fox CD6 is a target for cancer immunotherapy JCI Insight, 2021;0(0):. 2021 [PMID: 33497367] (Bioassay, Human) Bioassay Human
Show All 333 Publications.

Reviews for IL-2 (202-IL) (4) 54

Average Rating: 5
(Based on 4 reviews)

Reviews using 202-IL:
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CellProlif
(4)
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  5
reviewed by:
Verified Customer
CellProlif 05/16/2021
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Summary

ApplicationCell Proliferation
LotAE6420101
  5
reviewed by:
Verified Customer
CellProlif 03/31/2021
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Summary

ApplicationCell Proliferation
LotAE6419102
Commentshigh concentration.
  5
reviewed by:
Verified Customer
CellProlif 05/18/2019
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Summary

ApplicationCell Proliferation
LotAE6017121
  5
reviewed by:
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CellProlif 01/23/2019
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Summary

ApplicationCell Proliferation
Lot202

FAQs for IL-2 (202-IL). (Showing 1 - 1 of 1 FAQs).

  1. we bought the rHIL-2 protein ref 202-IL-500 and it says that the reconstitution has to be done in acetic acid, but I need to use the protein in PBS, is that possible to reconstitute it directly in PBS instead? would it be stable?
    • It will not be soluble in PBS alone so 100 mM acetic acid is required for it to go into solution. Then you can dilute into PBS only and that should not affect your experimentation.

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Recent Reviews

5
5
4
4
0
3
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2
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1
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Verified Customer
05/16/2021
Application: CellProlif
Species:

 
Verified Customer
03/31/2021
Application: CellProlif
Species:

 
Verified Customer
05/18/2019
Application: CellProlif
Species:

Bioinformatics

Gene Symbol IL2
Uniprot